Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmnc27j22
(357 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC1250.05 |rpl3002|rpl30-2, rpl30|60S ribosomal protein L30|Sc... 156 8e-40
SPAC9G1.03c |rpl3001|rpl30-1, rpl30|60S ribosomal protein L30|Sc... 153 1e-38
SPAC1834.01 |sup45||translation release factor eRF1|Schizosaccha... 29 0.16
SPAC977.14c |||aldo/keto reductase, unknown biological role|Schi... 28 0.49
SPAC1782.10c |nhp2||RNA-binding protein Nhp2 |Schizosaccharomyce... 26 1.5
SPBC244.02c |||U3 snoRNP-associated protein Utp6 |Schizosaccharo... 25 3.5
SPBPB21E7.09 ||SPAPB21E7.09, SPAPB21E7.09|L-asparaginase |Schizo... 24 6.1
SPAC14C4.13 |rad17||RFC related checkpoint protein Rad17|Schizos... 24 6.1
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 24 8.0
SPAC186.03 |||L-asparaginase|Schizosaccharomyces pombe|chr 1|||M... 24 8.0
>SPAC1250.05 |rpl3002|rpl30-2, rpl30|60S ribosomal protein
L30|Schizosaccharomyces pombe|chr 1|||Manual
Length = 117
Score = 156 bits (379), Expect = 8e-40
Identities = 72/104 (69%), Positives = 86/104 (82%)
Frame = +2
Query: 44 APKMVAAKKQKKTIESINSRLALVMKSGKYCLGYKQTLKTLRQGKAKLVIIAKNAPPLRK 223
AP +KK KK+ ++INS+LAL MKSGKY LGYK TLKTLR GKAKL++IA NAPPLRK
Sbjct: 8 APVAAVSKKGKKSGDTINSKLALTMKSGKYVLGYKSTLKTLRSGKAKLILIAANAPPLRK 67
Query: 224 SEIEYYALLAKTGVHHYSGNNIELGTACGKYYRVCTLALTDPGD 355
SE+EYYA+L++ VHHYSGNNI+LGTACGK +RV LA+ D GD
Sbjct: 68 SELEYYAMLSRCSVHHYSGNNIDLGTACGKLFRVGVLAVIDAGD 111
>SPAC9G1.03c |rpl3001|rpl30-1, rpl30|60S ribosomal protein
L30|Schizosaccharomyces pombe|chr 1|||Manual
Length = 109
Score = 153 bits (370), Expect = 1e-38
Identities = 69/100 (69%), Positives = 83/100 (83%)
Frame = +2
Query: 56 VAAKKQKKTIESINSRLALVMKSGKYCLGYKQTLKTLRQGKAKLVIIAKNAPPLRKSEIE 235
V KK KK+ ++IN++LAL MKSGKY LGYK TLKTLR GKAKL++IA N PPLRKSE+E
Sbjct: 4 VVTKKSKKSGDTINAKLALTMKSGKYVLGYKSTLKTLRSGKAKLILIAGNCPPLRKSELE 63
Query: 236 YYALLAKTGVHHYSGNNIELGTACGKYYRVCTLALTDPGD 355
YYA+L+K VHHY+G NI+LGTACGK +RV LA+TD GD
Sbjct: 64 YYAMLSKANVHHYAGTNIDLGTACGKLFRVGVLAITDAGD 103
>SPAC1834.01 |sup45||translation release factor
eRF1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 433
Score = 29.5 bits (63), Expect = 0.16
Identities = 13/42 (30%), Positives = 24/42 (57%)
Frame = +2
Query: 68 KQKKTIESINSRLALVMKSGKYCLGYKQTLKTLRQGKAKLVI 193
++KK I+ ++L SGKYC G T+ L++G + ++
Sbjct: 279 QEKKLIQRFFDEISL--DSGKYCFGVVDTMNALQEGAVETLL 318
>SPAC977.14c |||aldo/keto reductase, unknown biological
role|Schizosaccharomyces pombe|chr 1|||Manual
Length = 351
Score = 27.9 bits (59), Expect = 0.49
Identities = 15/45 (33%), Positives = 26/45 (57%)
Frame = +2
Query: 176 KAKLVIIAKNAPPLRKSEIEYYALLAKTGVHHYSGNNIELGTACG 310
++ +VI++K P+RK I+ + L+ GVH ++ EL CG
Sbjct: 87 RSSIVILSKCFFPVRKDLIKIFGDLSSRGVHFL--DSPELANQCG 129
>SPAC1782.10c |nhp2||RNA-binding protein Nhp2 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 154
Score = 26.2 bits (55), Expect = 1.5
Identities = 17/58 (29%), Positives = 32/58 (55%)
Frame = +2
Query: 44 APKMVAAKKQKKTIESINSRLALVMKSGKYCLGYKQTLKTLRQGKAKLVIIAKNAPPL 217
APK + KK KT++ + + ++ G K+ +K +R+G+ LVI+A + P+
Sbjct: 33 APKKLN-KKMMKTVKKASKQKHILR-------GVKEVVKAVRKGEKGLVILAGDISPM 82
>SPBC244.02c |||U3 snoRNP-associated protein Utp6
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 488
Score = 25.0 bits (52), Expect = 3.5
Identities = 10/22 (45%), Positives = 15/22 (68%)
Frame = -3
Query: 67 LCCNHFGSVNANESSPRAQMEK 2
+ C+H S+NAN S+ RA M +
Sbjct: 146 VACDHEFSINANVSAARALMNR 167
>SPBPB21E7.09 ||SPAPB21E7.09, SPAPB21E7.09|L-asparaginase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 360
Score = 24.2 bits (50), Expect = 6.1
Identities = 11/29 (37%), Positives = 19/29 (65%)
Frame = -1
Query: 261 PVLARRA*YSISDFLSGGAFLAMITSFAL 175
P+L ++ ++SDF+S A L +T FA+
Sbjct: 18 PLLFQKRSSNVSDFISFNASLPNVTIFAM 46
>SPAC14C4.13 |rad17||RFC related checkpoint protein
Rad17|Schizosaccharomyces pombe|chr 1|||Manual
Length = 606
Score = 24.2 bits (50), Expect = 6.1
Identities = 10/25 (40%), Positives = 15/25 (60%)
Frame = +3
Query: 33 LAFTLPKWLQQRNRKRPSSQLTPAL 107
L ++L +WL N K PS+Q + L
Sbjct: 129 LGYSLIEWLNPMNLKEPSNQESDTL 153
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 23.8 bits (49), Expect = 8.0
Identities = 11/32 (34%), Positives = 16/32 (50%)
Frame = -3
Query: 127 SRFHHQSKAGVN*LDGLFLFLCCNHFGSVNAN 32
+R ++ GV + LF CC FG+V N
Sbjct: 1143 NRINNSKPLGVFRIHDPSLFACCYQFGAVFVN 1174
>SPAC186.03 |||L-asparaginase|Schizosaccharomyces pombe|chr
1|||Manual
Length = 360
Score = 23.8 bits (49), Expect = 8.0
Identities = 12/29 (41%), Positives = 18/29 (62%)
Frame = -1
Query: 261 PVLARRA*YSISDFLSGGAFLAMITSFAL 175
P L ++ +ISDF+S A L +T FA+
Sbjct: 18 PFLFQKRSSNISDFISFNASLPNVTIFAM 46
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,497,142
Number of Sequences: 5004
Number of extensions: 26828
Number of successful extensions: 59
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 58
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 59
length of database: 2,362,478
effective HSP length: 65
effective length of database: 2,037,218
effective search space used: 107972554
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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