Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmnc27j04
(439 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 194 4e-51
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 100 8e-23
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 88 6e-19
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 81 7e-17
SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha... 75 6e-15
SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyce... 57 1e-09
SPAC18B11.04 |ncs1||related to neuronal calcium sensor Ncs1|Schi... 41 1e-04
SPBC1778.06c |fim1||fimbrin|Schizosaccharomyces pombe|chr 2|||Ma... 36 0.004
SPAC1687.14c |||EF hand family protein, unknown role|Schizosacch... 35 0.006
SPCC1183.11 ||SPCC31H12.01|MS ion channel protein 1|Schizosaccha... 28 0.55
SPAC694.06c |mrc1||mediator of replication checkpoint 1 |Schizos... 28 0.55
SPAC1327.01c ||SPAC1783.09c, SPAC18G6.16c|transcription factor, ... 27 1.7
SPCC320.04c |||GTPase Gem1 |Schizosaccharomyces pombe|chr 3|||Ma... 26 2.2
SPBC609.01 |||ribonuclease II |Schizosaccharomyces pombe|chr 2||... 26 2.2
SPAC4D7.13 |usp104|prp40|U1 snRNP-associated protein Usp104|Schi... 25 5.1
SPAC3C7.04 |||transcription factor |Schizosaccharomyces pombe|ch... 25 6.7
SPAPB21F2.02 |||Dopey family protein|Schizosaccharomyces pombe|c... 25 6.7
SPAC31A2.06 |||conserved fungal protein|Schizosaccharomyces pomb... 24 8.9
SPAPB1E7.02c |mcl1|slr3|DNA polymerase alpha accessory factor Mc... 24 8.9
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 24 8.9
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 194 bits (474), Expect = 4e-51
Identities = 90/111 (81%), Positives = 102/111 (91%)
Frame = +1
Query: 106 LTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTI 285
LT+EQIAEF+EAFSLFD+D DG IT+ ELG VMRSLGQ+PT AELQDMINEVDADGNGTI
Sbjct: 6 LTDEQIAEFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGTI 65
Query: 286 DFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGFISAAELRHVMTNLGE 438
DF EFLTMMARKMKDTD+EEE+REAF+VFDKDGNG+I+ EL HV+T+LGE
Sbjct: 66 DFTEFLTMMARKMKDTDNEEEVREAFKVFDKDGNGYITVEELTHVLTSLGE 116
Score = 70.1 bits (164), Expect = 1e-13
Identities = 34/76 (44%), Positives = 51/76 (67%)
Frame = +1
Query: 94 MADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADG 273
M D EE++ +EAF +FDKDG+G IT +EL V+ SLG+ ++ E+ DMI E D DG
Sbjct: 78 MKDTDNEEEV---REAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEVADMIREADTDG 134
Query: 274 NGTIDFPEFLTMMARK 321
+G I++ EF +++ K
Sbjct: 135 DGVINYEEFSRVISSK 150
Score = 57.2 bits (132), Expect = 1e-09
Identities = 34/103 (33%), Positives = 54/103 (52%), Gaps = 1/103 (0%)
Frame = +1
Query: 124 AEFKEAFSLFDKDGDGTITTKELGTVM-RSLGQNPTEAELQDMINEVDADGNGTIDFPEF 300
AE ++ + D DG+GTI E T+M R + E E+++ D DGNG I E
Sbjct: 48 AELQDMINEVDADGNGTIDFTEFLTMMARKMKDTDNEEEVREAFKVFDKDGNGYITVEE- 106
Query: 301 LTMMARKMKDTDSEEEIREAFRVFDKDGNGFISAAELRHVMTN 429
LT + + + S+EE+ + R D DG+G I+ E V+++
Sbjct: 107 LTHVLTSLGERLSQEEVADMIREADTDGDGVINYEEFSRVISS 149
>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 143
Score = 100 bits (240), Expect = 8e-23
Identities = 51/110 (46%), Positives = 73/110 (66%)
Frame = +1
Query: 109 TEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTID 288
++EQ E KEAF L+D D DG I T +G+V+RSLG N T+AEL + NE+ ID
Sbjct: 4 SKEQTDEMKEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNEL----GDAID 59
Query: 289 FPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGFISAAELRHVMTNLGE 438
+F++ ++ K+++T+SEEE +AFRVFDKD +G+I A+ M LGE
Sbjct: 60 EKKFMSFVSNKLRETESEEEYIKAFRVFDKDNSGYIETAKFADYMKTLGE 109
Score = 52.4 bits (120), Expect = 3e-08
Identities = 26/79 (32%), Positives = 47/79 (59%), Gaps = 1/79 (1%)
Frame = +1
Query: 88 STMADQLTE-EQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVD 264
S ++++L E E E+ +AF +FDKD G I T + M++LG+ ++ E+Q M+ E D
Sbjct: 65 SFVSNKLRETESEEEYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQLMVQEAD 124
Query: 265 ADGNGTIDFPEFLTMMARK 321
+G+ D+ +F+ + K
Sbjct: 125 PTNSGSFDYYDFVQRIMAK 143
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 87.8 bits (208), Expect = 6e-19
Identities = 44/112 (39%), Positives = 62/112 (55%)
Frame = +1
Query: 103 QLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGT 282
++TEEQ + EAF LFD D D I EL MR+LG N ++E+ ++ + D G G
Sbjct: 30 EITEEQRQDINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGKGY 89
Query: 283 IDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGFISAAELRHVMTNLGE 438
+ +F+ +M K+ + D EEI+ AF +FD D G IS LR V L E
Sbjct: 90 LQMEDFVRVMTEKIVERDPLEEIKRAFELFDDDETGKISLRNLRRVAKELNE 141
>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 141
Score = 81.0 bits (191), Expect = 7e-17
Identities = 43/105 (40%), Positives = 62/105 (59%), Gaps = 2/105 (1%)
Frame = +1
Query: 130 FKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTM 309
+K+AFSLFD+ G G I +G ++R+ GQNPT AE I E+++ +D +FL +
Sbjct: 8 YKQAFSLFDRHGTGRIPKTSIGDLLRACGQNPTLAE----ITEIESTLPAEVDMEQFLQV 63
Query: 310 MARK--MKDTDSEEEIREAFRVFDKDGNGFISAAELRHVMTNLGE 438
+ R EE + F+VFDKD G I ELR+V+T+LGE
Sbjct: 64 LNRPNGFDMPGDPEEFVKGFQVFDKDATGMIGVGELRYVLTSLGE 108
Score = 41.9 bits (94), Expect = 4e-05
Identities = 20/62 (32%), Positives = 36/62 (58%)
Frame = +1
Query: 127 EFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLT 306
EF + F +FDKD G I EL V+ SLG+ + E+ +++ V +G +++ +F+
Sbjct: 78 EFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELLKGVPVK-DGMVNYHDFVQ 136
Query: 307 MM 312
M+
Sbjct: 137 MI 138
>SPAC926.03 |rlc1||myosin II regulatory light chain
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 184
Score = 74.5 bits (175), Expect = 6e-15
Identities = 41/112 (36%), Positives = 62/112 (55%)
Frame = +1
Query: 103 QLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGT 282
QLT QI E KEAF+L DKDGDG I +++ T++ SL Q+ +E D IN + N
Sbjct: 41 QLTSSQIQELKEAFALLDKDGDGNIGREDVKTMLTSLNQDASE----DSINHMFESINPP 96
Query: 283 IDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGFISAAELRHVMTNLGE 438
I+ FLT M + ++ EAF FD +G I + +R ++++G+
Sbjct: 97 INLAAFLTAMGSMLCRISPRNDLLEAFSTFDDTQSGKIPISTMRDALSSMGD 148
Score = 25.8 bits (54), Expect = 2.9
Identities = 12/46 (26%), Positives = 20/46 (43%)
Frame = +1
Query: 136 EAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADG 273
EAFS FD G I + + S+G E++ ++ + G
Sbjct: 121 EAFSTFDDTQSGKIPISTMRDALSSMGDRMDPQEVESILRSYTSHG 166
>SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 174
Score = 57.2 bits (132), Expect = 1e-09
Identities = 32/105 (30%), Positives = 57/105 (54%)
Frame = +1
Query: 109 TEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTID 288
+ E+I ++ F D + G+I E ++ S+ NP + L + VD DG G +D
Sbjct: 19 SNEEIERIRKRFIKIDANQSGSIDRNEFLSIP-SVASNPLASRL---FSVVDEDGGGDVD 74
Query: 289 FPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGFISAAELRHVM 423
F EF+ ++ + EE+++ AF+++D D +G+IS EL V+
Sbjct: 75 FQEFINSLSVFSVHGNKEEKLKFAFKIYDIDRDGYISNGELYLVL 119
>SPAC18B11.04 |ncs1||related to neuronal calcium sensor
Ncs1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 190
Score = 40.7 bits (91), Expect = 1e-04
Identities = 20/59 (33%), Positives = 35/59 (59%)
Frame = +1
Query: 247 MINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGFISAAELRHVM 423
+ N DAD NG IDF EF+ ++ + + +++ AF+++D D NG IS E+ ++
Sbjct: 68 VFNVFDADKNGYIDFKEFICALSVTSRG-ELNDKLIWAFQLYDLDNNGLISYDEMLRIV 125
>SPBC1778.06c |fim1||fimbrin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 614
Score = 35.5 bits (78), Expect = 0.004
Identities = 19/51 (37%), Positives = 28/51 (54%)
Frame = +1
Query: 238 LQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNG 390
L D N++D DG G +D P T+ A + S +E+REA R + D +G
Sbjct: 21 LTDQFNKLDVDGKGYLDQP--TTIKAFEDSKKGSYDEVREAIREVNVDSSG 69
>SPAC1687.14c |||EF hand family protein, unknown
role|Schizosaccharomyces pombe|chr 1|||Manual
Length = 76
Score = 34.7 bits (76), Expect = 0.006
Identities = 23/62 (37%), Positives = 32/62 (51%)
Frame = +1
Query: 115 EQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFP 294
E E +EAF LFD G I ++L LG+N T+ +LQ M++ A NG +
Sbjct: 9 EMDEEAEEAFDLFDVTHKGYIDFEDLRRSCAQLGENLTKEQLQLMLDL--AGTNGKVSRE 66
Query: 295 EF 300
EF
Sbjct: 67 EF 68
Score = 32.3 bits (70), Expect = 0.034
Identities = 16/43 (37%), Positives = 22/43 (51%)
Frame = +1
Query: 310 MARKMKDTDSEEEIREAFRVFDKDGNGFISAAELRHVMTNLGE 438
M+ K + +EE EAF +FD G+I +LR LGE
Sbjct: 1 MSVSTKRLEMDEEAEEAFDLFDVTHKGYIDFEDLRRSCAQLGE 43
>SPCC1183.11 ||SPCC31H12.01|MS ion channel protein
1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1011
Score = 28.3 bits (60), Expect = 0.55
Identities = 15/56 (26%), Positives = 29/56 (51%), Gaps = 2/56 (3%)
Frame = +1
Query: 100 DQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLG--QNPTEAELQDMINEV 261
D+ E A + +S+FD+D +G IT +E+ +G + A L+D+ + +
Sbjct: 538 DETGEVDNATLEACYSIFDRDLNGDITCEEIELACVEIGKERKSISASLRDLNDSI 593
>SPAC694.06c |mrc1||mediator of replication checkpoint 1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1019
Score = 28.3 bits (60), Expect = 0.55
Identities = 12/43 (27%), Positives = 24/43 (55%)
Frame = +1
Query: 250 INEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDK 378
I+E D + NG + F + + +KD+ EE+ ++F + D+
Sbjct: 545 ISESDDESNGDMIRDSFDRLSSESIKDSQKTEELHDSFGINDE 587
>SPAC1327.01c ||SPAC1783.09c, SPAC18G6.16c|transcription factor,
zf-fungal binuclear cluster type |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 977
Score = 26.6 bits (56), Expect = 1.7
Identities = 9/27 (33%), Positives = 15/27 (55%)
Frame = -1
Query: 244 LEVRLLWGSVLATSSRCPALWS*WCHR 164
LE++ WGS+ A + +W+ W R
Sbjct: 850 LEMKNYWGSISALCDKMSEIWADWVQR 876
>SPCC320.04c |||GTPase Gem1 |Schizosaccharomyces pombe|chr
3|||Manual
Length = 630
Score = 26.2 bits (55), Expect = 2.2
Identities = 19/85 (22%), Positives = 31/85 (36%)
Frame = +1
Query: 136 EAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMA 315
+ F FD+D DG + +EL + R P N + +G + + +L +
Sbjct: 311 DLFYQFDRDNDGALNNEELSALFRHTPGLPEIWVSSQFPNSTVLNEHGYVTYNGWLAQWS 370
Query: 316 RKMKDTDSEEEIREAFRVFDKDGNG 390
A+ FD DG G
Sbjct: 371 MITLFDYKTTLAYLAYLGFDTDGRG 395
>SPBC609.01 |||ribonuclease II |Schizosaccharomyces pombe|chr
2|||Manual
Length = 1157
Score = 26.2 bits (55), Expect = 2.2
Identities = 16/70 (22%), Positives = 34/70 (48%), Gaps = 1/70 (1%)
Frame = +1
Query: 172 TITTKELGTVMRSLGQ-NPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEE 348
T + GT+ S+G + E + ++++ T DFPE L+ +++ EE
Sbjct: 545 TTSLHPFGTLYESIGTIGDPKVEYKAILHDFSCH---TYDFPESLSHCVKRLPTPIPAEE 601
Query: 349 IREAFRVFDK 378
+++ + + DK
Sbjct: 602 LQKRYNLRDK 611
>SPAC4D7.13 |usp104|prp40|U1 snRNP-associated protein
Usp104|Schizosaccharomyces pombe|chr 1|||Manual
Length = 695
Score = 25.0 bits (52), Expect = 5.1
Identities = 9/38 (23%), Positives = 19/38 (50%)
Frame = +1
Query: 244 DMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIRE 357
D + + + T + PE + ++ K+KD + E + E
Sbjct: 479 DCLEVLQISVDDTSNIPEIIARLSEKLKDREESEAVTE 516
>SPAC3C7.04 |||transcription factor |Schizosaccharomyces pombe|chr
1|||Manual
Length = 783
Score = 24.6 bits (51), Expect = 6.7
Identities = 20/63 (31%), Positives = 28/63 (44%), Gaps = 2/63 (3%)
Frame = +1
Query: 229 EAELQDMINEVDADGNGTIDFPEFLT--MMARKMKDTDSEEEIREAFRVFDKDGNGFISA 402
E L+ +N + + N ++D P FL MM + + EIR A FD G F
Sbjct: 281 ETLLRYRLNLIMSFQNPSVD-PGFLCLLMMIFALGSMFAHMEIRSASNPFDNPGKQFFDT 339
Query: 403 AEL 411
A L
Sbjct: 340 ARL 342
>SPAPB21F2.02 |||Dopey family protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1687
Score = 24.6 bits (51), Expect = 6.7
Identities = 10/37 (27%), Positives = 18/37 (48%)
Frame = -2
Query: 159 FVEQ*ECLLKLGDLFFRQLIRHCRLTWIIWSYTKKSQ 49
F E EC+ L ++ + C+ W I+S+ S+
Sbjct: 1113 FCEPLECVETLVQIYLENNVECCQCIWDIFSFLSDSE 1149
>SPAC31A2.06 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 542
Score = 24.2 bits (50), Expect = 8.9
Identities = 9/33 (27%), Positives = 18/33 (54%)
Frame = +1
Query: 142 FSLFDKDGDGTITTKELGTVMRSLGQNPTEAEL 240
F KD +TTK++ T+ ++ +PT+ +
Sbjct: 321 FERLRKDSSLQLTTKDINTLFSTIALSPTKLSM 353
>SPAPB1E7.02c |mcl1|slr3|DNA polymerase alpha accessory factor
Mcl1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 815
Score = 24.2 bits (50), Expect = 8.9
Identities = 20/57 (35%), Positives = 26/57 (45%)
Frame = +1
Query: 73 DNPS*STMADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQ 243
+NP ST L E Q+ L D GDG +T E ++ R L N +A LQ
Sbjct: 707 NNPDASTSVPVLEELQLRNKLFLTLLEDSIGDGDVTEDEKISIAR-LEANIDKALLQ 762
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 24.2 bits (50), Expect = 8.9
Identities = 11/20 (55%), Positives = 13/20 (65%)
Frame = -1
Query: 343 PRYPCPSSCAPSLSRTRESL 284
P P PSS APS+S R S+
Sbjct: 1237 PPVPAPSSEAPSVSTPRSSV 1256
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.316 0.134 0.372
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,445,235
Number of Sequences: 5004
Number of extensions: 25815
Number of successful extensions: 120
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 99
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 109
length of database: 2,362,478
effective HSP length: 67
effective length of database: 2,027,210
effective search space used: 158122380
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.6 bits)
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