Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmnc27i10
(499 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC1B3.14 |vma3||V-type ATPase subunit c|Schizosaccharomyces po... 174 5e-45
SPAC732.01 |vma11||V-type ATPase proteolipid subunit|Schizosacch... 154 8e-39
SPAC2C4.13 |vma16||V-type ATPase subunit c''|Schizosaccharomyces... 70 2e-13
SPAC212.04c |||S. pombe specific DUF999 family protein 1|Schizos... 31 0.096
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 31 0.13
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 28 0.68
SPAC227.06 |||Rab GTPase binding |Schizosaccharomyces pombe|chr ... 27 1.2
SPBC19G7.05c |bgs1|cps1, drc1|1,3-beta-glucan synthase catalytic... 27 1.6
SPBC17G9.05 |rct1|cyp6|RRM-containing cyclophilin regulating tra... 27 2.1
SPBC25D12.03c |mcm7||MCM complex subunit Mcm7|Schizosaccharomyce... 26 2.7
SPBC691.04 |||mitochondrial ATP-dependent RNA helicase Mss116 |S... 25 4.8
SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1... 25 4.8
SPBC651.08c |rpc1||DNA-directed RNA polymerase III complex large... 25 6.3
SPBC15C4.06c ||SPBC21H7.01c|ubiquitin-protein ligase E3 |Schizos... 25 8.4
>SPAC1B3.14 |vma3||V-type ATPase subunit c|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 161
Score = 174 bits (424), Expect = 5e-45
Identities = 84/117 (71%), Positives = 102/117 (87%)
Frame = +3
Query: 147 PIYGPFFGVMGAASAIIFSALGAAYGTAKSGTGIAAMSVMRPELIMKSIIPVVMAGIIAI 326
P+Y PFFGVMG +AI+F++ GAAYGTAK+G GI+AM V+RP+LI+K+ IPVVMAGIIAI
Sbjct: 7 PVYAPFFGVMGCTAAIVFASFGAAYGTAKAGVGISAMGVLRPDLIVKNTIPVVMAGIIAI 66
Query: 327 YGLVVAVLIAGALQEPANYPLYKGFIHLGAGLAVGFSGLAAGFAIGIVGDAGVRGTA 497
YGLVV+VLI+G L++ + LY GFI LGAGL+VG +GLAAGFAIGIVGDAGVRGTA
Sbjct: 67 YGLVVSVLISGNLKQILS--LYSGFIQLGAGLSVGLAGLAAGFAIGIVGDAGVRGTA 121
>SPAC732.01 |vma11||V-type ATPase proteolipid
subunit|Schizosaccharomyces pombe|chr 1|||Manual
Length = 162
Score = 154 bits (373), Expect = 8e-39
Identities = 69/114 (60%), Positives = 90/114 (78%)
Frame = +3
Query: 147 PIYGPFFGVMGAASAIIFSALGAAYGTAKSGTGIAAMSVMRPELIMKSIIPVVMAGIIAI 326
PIY FFG G ++++FS LGA YGTA +G GIAA+ RPE++MKS+IPVVM+GII +
Sbjct: 7 PIYSSFFGFAGVCASMVFSCLGAGYGTALAGRGIAAVGAFRPEIVMKSLIPVVMSGIIGV 66
Query: 327 YGLVVAVLIAGALQEPANYPLYKGFIHLGAGLAVGFSGLAAGFAIGIVGDAGVR 488
YGLV++VLIAG + +Y L+ GFIHL AGLAVG +G+AAG+AIG+VGD GV+
Sbjct: 67 YGLVMSVLIAGDMSPDNDYSLFSGFIHLSAGLAVGLTGVAAGYAIGVVGDRGVQ 120
>SPAC2C4.13 |vma16||V-type ATPase subunit c''|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 199
Score = 69.7 bits (163), Expect = 2e-13
Identities = 35/117 (29%), Positives = 60/117 (51%), Gaps = 6/117 (5%)
Frame = +3
Query: 165 FGVMGAASAIIFSALGAAYGTAKSGTGIAAMSVMRPELIMKSIIPVVMAGIIAIYGLVVA 344
+G++G AS + F +GAA+G GT I +V P + K++I ++ ++AIY L++A
Sbjct: 45 WGLLGIASCVAFGIIGAAWGIFICGTSILGGAVKAPRIKTKNLISIIFCEVVAIYSLIIA 104
Query: 345 VLIAGALQE--PANY----PLYKGFIHLGAGLAVGFSGLAAGFAIGIVGDAGVRGTA 497
++ + + + PA + Y GF G+ VG L G +GI G + A
Sbjct: 105 IVFSAKINDINPAGFYTKSHYYTGFALFWGGITVGLCNLICGVCVGITGSSAALADA 161
>SPAC212.04c |||S. pombe specific DUF999 family protein
1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 288
Score = 31.1 bits (67), Expect = 0.096
Identities = 21/68 (30%), Positives = 30/68 (44%)
Frame = +3
Query: 291 IIPVVMAGIIAIYGLVVAVLIAGALQEPANYPLYKGFIHLGAGLAVGFSGLAAGFAIGIV 470
II +AG+IA + +++ IAG + G ++ G L LA GF I
Sbjct: 193 IITATIAGVIAAFSVIITATIAGVIAAMV------GILYFGHWLVYKILILAFGFKIVTS 246
Query: 471 GDAGVRGT 494
GD V T
Sbjct: 247 GDVCVSNT 254
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 30.7 bits (66), Expect = 0.13
Identities = 14/34 (41%), Positives = 18/34 (52%)
Frame = -1
Query: 496 AVPRTPASPTMPMAKPAARPENPTAKPAPKWMNP 395
+VP P++P MP P+A P A AP NP
Sbjct: 1713 SVPPPPSAPPMPAGPPSAPPPPLPASSAPSVPNP 1746
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 28.3 bits (60), Expect = 0.68
Identities = 26/65 (40%), Positives = 30/65 (46%), Gaps = 2/65 (3%)
Frame = -1
Query: 496 AVPRTPAS--PTMPMAKPAARPENPTAKPAPKWMNPL*RG*LAGSWRAPAIRTATTRP*M 323
A P P S P++PM PAA P P+A AP PL AG AP + A P
Sbjct: 427 APPSLPPSAPPSLPMGAPAAPPLPPSAPIAP----PL----PAGMPAAPPLPPAAPAPPP 478
Query: 322 AIIPA 308
A PA
Sbjct: 479 APAPA 483
>SPAC227.06 |||Rab GTPase binding |Schizosaccharomyces pombe|chr
1|||Manual
Length = 249
Score = 27.5 bits (58), Expect = 1.2
Identities = 22/72 (30%), Positives = 32/72 (44%)
Frame = +3
Query: 150 IYGPFFGVMGAASAIIFSALGAAYGTAKSGTGIAAMSVMRPELIMKSIIPVVMAGIIAIY 329
+YGPF+ A+ FS Y +G G + S+ K +I A II Y
Sbjct: 87 LYGPFWITTTVIQALFFSNSITEYARYATGHGTSGYSI-------KKLISA--ASIIYGY 137
Query: 330 GLVVAVLIAGAL 365
++AVL+ G L
Sbjct: 138 TTIIAVLLWGIL 149
>SPBC19G7.05c |bgs1|cps1, drc1|1,3-beta-glucan synthase catalytic
subunit Bgs1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1729
Score = 27.1 bits (57), Expect = 1.6
Identities = 21/95 (22%), Positives = 47/95 (49%), Gaps = 6/95 (6%)
Frame = +3
Query: 126 NKMAENNP---IYGPFFG-VMGAASAIIFSALGAAYGTAKSGTGIAAMSVMR-PELIMKS 290
+K+A ++P I F+G ++G + F+ + + ++ G S LI+ S
Sbjct: 1424 DKIAMDSPRARITTMFYGEILGPLGTLFFTCIPFLFINSQPGNDDETQSTNAFIRLIIMS 1483
Query: 291 IIPVVMAGIIAIYGLVVAVLIAGALQEPA-NYPLY 392
+ P+V++ IIA + + +++ L + + Y +Y
Sbjct: 1484 VAPLVLSAIIAFFFFCLGIMLRPILGDRSKTYGVY 1518
>SPBC17G9.05 |rct1|cyp6|RRM-containing cyclophilin regulating
transcription Rct1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 432
Score = 26.6 bits (56), Expect = 2.1
Identities = 10/23 (43%), Positives = 14/23 (60%)
Frame = -2
Query: 105 TGPKNCDDYLHTLTPRNYFCTDK 37
TG KNCDD+L +P + D+
Sbjct: 402 TGNKNCDDHLRDKSPERRYRYDR 424
>SPBC25D12.03c |mcm7||MCM complex subunit Mcm7|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 760
Score = 26.2 bits (55), Expect = 2.7
Identities = 14/38 (36%), Positives = 22/38 (57%)
Frame = -3
Query: 404 DEPFVKGVVGWLLEGTSNQDSHDQTVDGNNTRHDDRND 291
DEPF+K +L+ SN++S+ VD N+ D +D
Sbjct: 48 DEPFLKSKYMDILQKISNRESNVINVDLNDLYEFDPSD 85
>SPBC691.04 |||mitochondrial ATP-dependent RNA helicase Mss116
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 535
Score = 25.4 bits (53), Expect = 4.8
Identities = 8/13 (61%), Positives = 10/13 (76%)
Frame = -2
Query: 105 TGPKNCDDYLHTL 67
TGP N DDY+H +
Sbjct: 369 TGPSNTDDYIHRI 381
>SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 857
Score = 25.4 bits (53), Expect = 4.8
Identities = 13/34 (38%), Positives = 19/34 (55%), Gaps = 4/34 (11%)
Frame = -1
Query: 496 AVPRTPASPTMPMA----KPAARPENPTAKPAPK 407
+VP+ PA+P +P A +P A P P AP+
Sbjct: 513 SVPQPPAAPVVPEAPSVHQPPAAPVAPEVPSAPQ 546
>SPBC651.08c |rpc1||DNA-directed RNA polymerase III complex large
subunit Rpc1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1405
Score = 25.0 bits (52), Expect = 6.3
Identities = 8/19 (42%), Positives = 13/19 (68%)
Frame = -2
Query: 471 PRCLWRNRQPDQRILQPNQ 415
PRCLW +Q +++PN+
Sbjct: 582 PRCLWTGKQVFTVLMKPNR 600
>SPBC15C4.06c ||SPBC21H7.01c|ubiquitin-protein ligase E3
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 556
Score = 24.6 bits (51), Expect = 8.4
Identities = 13/54 (24%), Positives = 26/54 (48%)
Frame = +3
Query: 204 ALGAAYGTAKSGTGIAAMSVMRPELIMKSIIPVVMAGIIAIYGLVVAVLIAGAL 365
AL +G + + + P+L+ I V+ G+I + GL + + +GA+
Sbjct: 354 ALVEYFGNIDATARLGVIISKEPKLLGLYIFIGVLLGLIGVIGLFICLHFSGAM 407
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,308,809
Number of Sequences: 5004
Number of extensions: 48718
Number of successful extensions: 185
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 176
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 182
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 196153982
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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