Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= bmnc27f03
         (565 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC20G4.06c |adf1|cof1|cofilin|Schizosaccharomyces pombe|chr 1|...   100   3e-22
SPCC126.06 |twf1||twinfilin|Schizosaccharomyces pombe|chr 3|||Ma...    38   0.001
SPAC17H9.11 |||cofilin/tropomyosin family protein|Schizosaccharo...    29   0.36 
SPBP4H10.18c |||sequence orphan|Schizosaccharomyces pombe|chr 2|...    29   0.62 
SPCC1450.04 |tef5||translation elongation factor EF-1 beta subun...    27   1.9  
SPBP35G2.03c |sgo1||shugoshin Sgo1|Schizosaccharomyces pombe|chr...    26   3.3  
SPAC1D4.11c |lkh1|kic1|dual specificity protein kinase Lkh1|Schi...    26   4.4  
SPAC29A4.10 |rrn5||RNA polymerase I upstream activation factor c...    25   5.8  
SPCC1742.01 ||SPCC1795.13, SPCPB16A4.07c|sequence orphan|Schizos...    25   5.8  
SPBC3H7.05c |||sequence orphan|Schizosaccharomyces pombe|chr 2||...    25   5.8  
SPAC9.07c |||GTPase Rbg1 |Schizosaccharomyces pombe|chr 1|||Manual     25   7.7  
SPBP26C9.03c |||iron ion transporter |Schizosaccharomyces pombe|...    25   7.7  

>SPAC20G4.06c |adf1|cof1|cofilin|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 137

 Score = 99.5 bits (237), Expect = 3e-22
 Identities = 55/140 (39%), Positives = 81/140 (57%)
 Frame = +1

Query: 64  SGVTVSDACKTTYEEIKKDKKHRYVVFYIRDEKQIDVETVGERNAEYEQFLEDLQKGGTG 243
           SGV VS  C   ++E+K  K  RYVVF + D K   V      + +++ FL DL +    
Sbjct: 4   SGVKVSPECLEAFQELKLGKSLRYVVFKMNDTKTEIVVEKKSTDKDFDTFLGDLPEK--- 60

Query: 244 ECRYGLFDFEYTHQCQGTSEASKKQKLFLMSWCPDTAKVKKKMLYSSSFDALKKSLVGVQ 423
           +CRY ++DFE+ +  +G      + K+  +SW PD A +K KM+YSSS D L+++  G+ 
Sbjct: 61  DCRYAIYDFEF-NLGEGV-----RNKIIFISWSPDVAPIKSKMVYSSSKDTLRRAFTGIG 114

Query: 424 KYIQATDLSEASQEAVEEKL 483
             IQATD SE + E V EK+
Sbjct: 115 TDIQATDFSEVAYETVLEKV 134


>SPCC126.06 |twf1||twinfilin|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 328

 Score = 37.9 bits (84), Expect = 0.001
 Identities = 28/86 (32%), Positives = 44/86 (51%)
 Frame = +1

Query: 130 RYVVFYIRDEKQIDVETVGERNAEYEQFLEDLQKGGTGECRYGLFDFEYTHQCQGTSEAS 309
           R  +  I +E   DV+T+ E++   E    D +K    EC  G  +  +        + S
Sbjct: 26  RAAIISISNENSFDVKTMVEKSESIES---DFKK--VRECLLGSEEPAFVL----VYDDS 76

Query: 310 KKQKLFLMSWCPDTAKVKKKMLYSSS 387
           KK  L L+S+ P+ A V++KMLY+SS
Sbjct: 77  KKNLLQLISYVPENANVRRKMLYASS 102



 Score = 28.7 bits (61), Expect = 0.62
 Identities = 16/56 (28%), Positives = 32/56 (57%), Gaps = 5/56 (8%)
 Frame = +1

Query: 319 KLFLMSWCPDTAKVKKKMLYSSS----FDALKKSL-VGVQKYIQATDLSEASQEAV 471
           K+  +  CP  A VK +M+YSSS     D++K  L + +   I++ D ++ +++ +
Sbjct: 244 KILFIYICPMQATVKHRMVYSSSKLGLLDSIKAELGIVIDGKIESNDAADITEKEI 299


>SPAC17H9.11 |||cofilin/tropomyosin family
           protein|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 141

 Score = 29.5 bits (63), Expect = 0.36
 Identities = 17/63 (26%), Positives = 30/63 (47%)
 Frame = +1

Query: 295 TSEASKKQKLFLMSWCPDTAKVKKKMLYSSSFDALKKSLVGVQKYIQATDLSEASQEAVE 474
           T++      LF++ W P        M+Y+S+     + +  V K  +A D  + + EAV+
Sbjct: 79  TTDGRLSTPLFMIYWRPSATPNDLSMIYASA-KVWFQDVSQVHKVFEARDSEDITSEAVD 137

Query: 475 EKL 483
           E L
Sbjct: 138 EFL 140


>SPBP4H10.18c |||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 314

 Score = 28.7 bits (61), Expect = 0.62
 Identities = 37/174 (21%), Positives = 67/174 (38%), Gaps = 3/174 (1%)
 Frame = +1

Query: 37  LRE*HQKMASGVTVSDACKTTYEEIKKDKKHRYVVFYIRDEKQIDVETVGE-RNAEYEQF 213
           LR    ++  G    D+  ++ +E  ++KK +   F  RD++++ ++     R       
Sbjct: 41  LRSEGSELIFGGRSFDSLLSSIKETMEEKKKKSSSFEKRDKRRVQLKEKSPLRTPRNRTQ 100

Query: 214 LEDLQ--KGGTGECRYGLFDFEYTHQCQGTSEASKKQKLFLMSWCPDTAKVKKKMLYSSS 387
           + D +  +  T        +F+   Q   T  A+K     +    P T    K  + ++ 
Sbjct: 101 IIDARSTRKDTENESLRKNNFKRRTQFAFTGRATKSNAGVMDVQSPSTMSTSKNNVRNAE 160

Query: 388 FDALKKSLVGVQKYIQATDLSEASQEAVEEKLRATDRQ*TAFTHELATKPNPLS 549
             A KK + G +KY    + S    +  E K R  DR  + FT         LS
Sbjct: 161 RPASKKPVFGSKKYFDVINDSNVENKE-ETKDRNLDRALSKFTQSREKNEQNLS 213


>SPCC1450.04 |tef5||translation elongation factor EF-1 beta subunit
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 214

 Score = 27.1 bits (57), Expect = 1.9
 Identities = 10/26 (38%), Positives = 19/26 (73%)
 Frame = +1

Query: 385 SFDALKKSLVGVQKYIQATDLSEASQ 462
           S +AL++ L G + Y+Q+TD++  S+
Sbjct: 188 SLEALQEELEGFEDYVQSTDIAAMSK 213


>SPBP35G2.03c |sgo1||shugoshin Sgo1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 319

 Score = 26.2 bits (55), Expect = 3.3
 Identities = 26/136 (19%), Positives = 60/136 (44%), Gaps = 1/136 (0%)
 Frame = +1

Query: 91  KTTYEEIKKDKKHRYVVFYIRDEKQIDVETVGERNAEYEQFLEDLQKGGTGECRYGLFDF 270
           K + + ++ + K ++     +D K+ D + +  +  EY  F ++L+   TG+  Y   DF
Sbjct: 108 KCSSDFLEANVKSQFTTCENKDSKE-DYQILHNKRLEYVSFNDELKSLETGQPLYCFQDF 166

Query: 271 EYTHQCQGTSEASKKQKLFLMSWCPDTAKVKKKMLYSSSFDALKKSLVGVQKYIQATDLS 450
           +   +  G    S+K    ++      A V K      ++   +K++    K ++  +  
Sbjct: 167 Q--KKVHGPPALSEKPGKCILK-DKTNAHVNKIPQDEVNYSLPQKNITIFSKELKENEFE 223

Query: 451 EASQ-EAVEEKLRATD 495
             ++ E  EEK + ++
Sbjct: 224 SINEGETEEEKAKTSN 239


>SPAC1D4.11c |lkh1|kic1|dual specificity protein kinase
           Lkh1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 690

 Score = 25.8 bits (54), Expect = 4.4
 Identities = 12/44 (27%), Positives = 23/44 (52%), Gaps = 5/44 (11%)
 Frame = -3

Query: 338 HDIRKSFCFLLASDVPWHWCVYSKSNRPY-----LHSPVPPFCR 222
           HD+     +++ S   +H C  ++S  P+     LH+P+P  C+
Sbjct: 107 HDVASHPSYMVQSPTSYHACSNNQSPFPHSHHPPLHNPLPVSCQ 150


>SPAC29A4.10 |rrn5||RNA polymerase I upstream activation factor
           complex subunit Rrn5|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 556

 Score = 25.4 bits (53), Expect = 5.8
 Identities = 22/92 (23%), Positives = 39/92 (42%), Gaps = 4/92 (4%)
 Frame = -3

Query: 554 VSESGFGFVASSCVNAVYWRSVARSFSSTAS*DASERSVAWMYF*TPTR---DFFRASNE 384
           + E  +G ++ SCV+  YW +  +     A     +R +  + +  P++      R  N 
Sbjct: 56  LEEEQWGVLSGSCVDGTYWSAEEKELFFQAVARNGKRDLDLIAYSIPSKSAVQIERYINA 115

Query: 383 LEYNIFFLTLAVSGHHDIR-KSFCFLLASDVP 291
           LE  + +L      H D   +S C L   D+P
Sbjct: 116 LENELRWL----RNHVDASVRSQCLLKYEDIP 143


>SPCC1742.01 ||SPCC1795.13, SPCPB16A4.07c|sequence
           orphan|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 1563

 Score = 25.4 bits (53), Expect = 5.8
 Identities = 12/21 (57%), Positives = 12/21 (57%)
 Frame = -1

Query: 358 PWRCLGTTTSGRASVSC*PPT 296
           P   L  TTSG  S SC PPT
Sbjct: 172 PATVLIVTTSGSTSTSCPPPT 192


>SPBC3H7.05c |||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 357

 Score = 25.4 bits (53), Expect = 5.8
 Identities = 13/46 (28%), Positives = 20/46 (43%), Gaps = 2/46 (4%)
 Frame = -3

Query: 290 WHWCVYSKSNRPY--LHSPVPPFCRSSRNCSYSALRSPTVSTSICF 159
           W++  Y+ S R Y      VPP+CR         +R+     + CF
Sbjct: 122 WNFVDYALSLRKYGWTDDVVPPYCRKVLTYKSLGIRTAVYVGAFCF 167


>SPAC9.07c |||GTPase Rbg1 |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 366

 Score = 25.0 bits (52), Expect = 7.7
 Identities = 10/22 (45%), Positives = 11/22 (50%)
 Frame = +1

Query: 73  TVSDACKTTYEEIKKDKKHRYV 138
           TV D C   +  IK   KH YV
Sbjct: 316 TVEDFCNNIHSSIKSQFKHAYV 337


>SPBP26C9.03c |||iron ion transporter |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 584

 Score = 25.0 bits (52), Expect = 7.7
 Identities = 14/35 (40%), Positives = 19/35 (54%)
 Frame = +1

Query: 70  VTVSDACKTTYEEIKKDKKHRYVVFYIRDEKQIDV 174
           VT  D  KT  +  +K  KH+ V  YIR  K +D+
Sbjct: 113 VTNDDGTKTKVDSDEKKHKHKNVRDYIR-FKHVDI 146


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,043,346
Number of Sequences: 5004
Number of extensions: 37962
Number of successful extensions: 132
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 127
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 129
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 238029836
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)