Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmnc26p03
(677 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC227.05 |||prefoldin subunit 4|Schizosaccharomyces pombe|chr ... 53 4e-08
SPAC1687.10 |mcp1||sequence orphan|Schizosaccharomyces pombe|chr... 33 0.038
SPCC162.08c |nup211||nuclear pore complex associated protein|Sch... 33 0.038
SPCC285.16c |msh6||MutS protein homolog|Schizosaccharomyces pomb... 31 0.12
SPAC1556.01c |rad50|SPAP4C9.01c|DNA repair protein Rad50|Schizos... 31 0.20
SPAC6G9.10c |sen1||ATP-dependent 5' to 3' DNA/RNA helicase Sen1|... 30 0.35
SPAC27D7.02c |||GRIP domain protein|Schizosaccharomyces pombe|ch... 29 0.47
SPAC1F3.06c |spo15||sporulation protein Spo15|Schizosaccharomyce... 29 0.47
SPAC6G9.06c |pcp1||pericentrin Pcp1|Schizosaccharomyces pombe|ch... 29 0.62
SPBC29A10.13 |atp7||F0-ATPase subunit D|Schizosaccharomyces pomb... 29 0.82
SPAC7D4.04 |taf1||Taz1 interacting factor 1|Schizosaccharomyces ... 28 1.4
SPCC63.11 |prp28||U5 snRNP-associated protein Prp28 |Schizosacch... 28 1.4
SPAC144.14 |klp8||kinesin-like protein Klp8|Schizosaccharomyces ... 28 1.4
SPBC365.05c |slu7||splicing factor Slu7|Schizosaccharomyces pomb... 27 1.9
SPAC15A10.16 |bud6|aip3, fat1, SPAC15E1.01|actin interacting pro... 27 1.9
SPBC21D10.10 |||bromodomain protein|Schizosaccharomyces pombe|ch... 27 2.5
SPCC970.10c |brl2|rfp1|ubiquitin-protein ligase E3 Brl2|Schizosa... 26 4.4
SPBC1289.01c |chr4|cfh3, SPBC1539.11c|chitin synthase regulatory... 26 4.4
SPAC27F1.02c |cdc8|fus4|tropomyosin|Schizosaccharomyces pombe|ch... 26 5.8
SPBC29A3.13 |||PWWP domain protein|Schizosaccharomyces pombe|chr... 26 5.8
SPBC543.09 |||mitochondrial m-AAA protease|Schizosaccharomyces p... 25 7.6
SPBC18E5.03c |sim4||kinetochore protein Sim4 |Schizosaccharomyce... 25 7.6
>SPAC227.05 |||prefoldin subunit 4|Schizosaccharomyces pombe|chr
1|||Manual
Length = 123
Score = 52.8 bits (121), Expect = 4e-08
Identities = 31/117 (26%), Positives = 65/117 (55%), Gaps = 3/117 (2%)
Frame = +2
Query: 116 VHISYEDQQKINKFARLNAKVDDYKDELKVKQNDVKNLEEAVEELSL--ADDSEKIPYL- 286
V + EDQ+ +N+F+ L+++ + ++K + ++++ +A E L DD + IP L
Sbjct: 4 VPVLAEDQRNLNEFSVLHSRKAIQELDIKNLKTQIEDIVDAKNECELLDEDDGDDIPALK 63
Query: 287 IGEIFICQNLEITLKNLEEAKAKKIHEIHDLEEKCEELKSQMSDLKAHLYGKFGSHI 457
+G+ F +L + L LE+++ ++ L E+ ++++ +LK+ LY KF I
Sbjct: 64 VGDAFFQVSLPVLLDQLEQSEESLEKQVDVLRSSMEKDETRIQELKSMLYSKFHDQI 120
>SPAC1687.10 |mcp1||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 661
Score = 33.1 bits (72), Expect = 0.038
Identities = 23/79 (29%), Positives = 46/79 (58%), Gaps = 3/79 (3%)
Frame = +2
Query: 206 KQNDVKNLEEAVEELS--LADDSEKIPYLIGEIFICQNLEITLKNLEEAKAKKIHEIHDL 379
K+N+ KN+ + +E LS L + +++ ++ G+ +I L+ L AK ++H+I+ L
Sbjct: 106 KKNEAKNMYKEIERLSVILGESVDEVNFVNGDFEDIS--DIKLEQL-SAKLNELHQIYRL 162
Query: 380 -EEKCEELKSQMSDLKAHL 433
+EK E+K ++ L+ L
Sbjct: 163 RKEKNIEIKEELKKLQDSL 181
>SPCC162.08c |nup211||nuclear pore complex associated
protein|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1837
Score = 33.1 bits (72), Expect = 0.038
Identities = 26/102 (25%), Positives = 50/102 (49%), Gaps = 3/102 (2%)
Frame = +2
Query: 137 QQKINKFARLN-AKVDDYKDELKVKQNDVKNLEEAVEELSLADDS--EKIPYLIGEIFIC 307
Q +NK+ R++ ++++ K + + + + LE ++E + D+ +++ L E+
Sbjct: 1340 QSVLNKYERVDPTQLEELKKNCEALEKEKQELETKLQETAKETDTFKQQVNSLNEEV--- 1396
Query: 308 QNLEITLKNLEEAKAKKIHEIHDLEEKCEELKSQMSDLKAHL 433
+NL+ K +E+A K EKCE LK AHL
Sbjct: 1397 ENLK---KEVEQANTKNTRLAAAWNEKCENLKKSSLTRFAHL 1435
Score = 26.2 bits (55), Expect = 4.4
Identities = 11/31 (35%), Positives = 18/31 (58%)
Frame = +2
Query: 161 RLNAKVDDYKDELKVKQNDVKNLEEAVEELS 253
R AK+ Y+ + + QN + LEE +E L+
Sbjct: 1673 RSKAKISVYEKKTRDLQNKITQLEETIENLN 1703
>SPCC285.16c |msh6||MutS protein homolog|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1254
Score = 31.5 bits (68), Expect = 0.12
Identities = 25/112 (22%), Positives = 52/112 (46%), Gaps = 2/112 (1%)
Frame = +2
Query: 89 KGTFQPDSDVHISYEDQQKINKFAR--LNAKVDDYKDELKVKQNDVKNLEEAVEELSLAD 262
+G F+P+ Y+ QK + L A ++ YK +L+ + KN+ + V ++ +
Sbjct: 827 EGVFEPEIGFEAEYDTSQKYQSELKNELYALLEQYKKQLRCSSLNFKNIGKEVYQVEVPS 886
Query: 263 DSEKIPYLIGEIFICQNLEITLKNLEEAKAKKIHEIHDLEEKCEELKSQMSD 418
D K+P ++ + T + + KKI ++ + EE + S+M +
Sbjct: 887 D-VKVPVNWCKM---SGTKKTNRYYNDELRKKIKKLLEAEELHLAIMSRMQE 934
>SPAC1556.01c |rad50|SPAP4C9.01c|DNA repair protein
Rad50|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1290
Score = 30.7 bits (66), Expect = 0.20
Identities = 34/125 (27%), Positives = 58/125 (46%), Gaps = 4/125 (3%)
Frame = +2
Query: 62 LIVNMSTTAKGTFQPDSDVHISYEDQQKINKFARLNAKVDDYKDELKVKQNDVKNLEEAV 241
++ N T K +F S+V +SYE + N L+ V+D + +L+ K+ +L
Sbjct: 540 ILANQLMTLKSSF---SEV-MSYELKDDDNYNEELDKLVEDVRKKLQEKEEAESSLRSVR 595
Query: 242 EELSLADDSEKIPYLIGEI-FICQNLEI---TLKNLEEAKAKKIHEIHDLEEKCEELKSQ 409
E L + +I + I + +N +I TLK+ A I EI LE + EE +
Sbjct: 596 ERLEI-----RISLSVQSINDLTENKKIKTKTLKSYSGTFASMISEIKALESEIEENRKT 650
Query: 410 MSDLK 424
+ L+
Sbjct: 651 LHSLQ 655
>SPAC6G9.10c |sen1||ATP-dependent 5' to 3' DNA/RNA helicase
Sen1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1687
Score = 29.9 bits (64), Expect = 0.35
Identities = 28/94 (29%), Positives = 48/94 (51%), Gaps = 4/94 (4%)
Frame = +2
Query: 140 QKINKFAR-LNAKVDDYKDELKVKQN--DVKNLEEAVE-ELSLADDSEKIPYLIGEIFIC 307
+ IN R L+ + K L+V Q D+ +L+E + D +KI L +I +
Sbjct: 1234 ETINVSVRDLSLEYQTEKQLLEVNQGAIDLGSLQELTRWRDTFYDCIQKIEELEKQIDVA 1293
Query: 308 QNLEITLKNLEEAKAKKIHEIHDLEEKCEELKSQ 409
+++ K+L + KI+E + E+K EEL+SQ
Sbjct: 1294 RDVAEDTKSLGKELQNKINEKNLAEQKVEELQSQ 1327
>SPAC27D7.02c |||GRIP domain protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 750
Score = 29.5 bits (63), Expect = 0.47
Identities = 24/107 (22%), Positives = 45/107 (42%)
Frame = +2
Query: 98 FQPDSDVHISYEDQQKINKFARLNAKVDDYKDELKVKQNDVKNLEEAVEELSLADDSEKI 277
FQ + + + + K+++ +D +L QN+V ++ ++E + I
Sbjct: 27 FQEEDQLRRNNKSSNKLSQNEEDAKNMDSVVQKLNELQNNVVAFQKLLQEKT---PLSSI 83
Query: 278 PYLIGEIFICQNLEITLKNLEEAKAKKIHEIHDLEEKCEELKSQMSD 418
L G +NLE + + HE++DL+ E LK Q D
Sbjct: 84 QDLEGFREFMENLEHRYEMTVSEVRRLSHEVNDLQTDRENLKHQFED 130
Score = 25.4 bits (53), Expect = 7.6
Identities = 16/51 (31%), Positives = 26/51 (50%), Gaps = 2/51 (3%)
Frame = +2
Query: 128 YEDQ-QKIN-KFARLNAKVDDYKDELKVKQNDVKNLEEAVEELSLADDSEK 274
+EDQ +K+N + + N+ + KDEL+ LEE + L A E+
Sbjct: 128 FEDQIEKLNSEISNQNSLILQKKDELEKSIQRCSELEEKINSLESAQSIEQ 178
>SPAC1F3.06c |spo15||sporulation protein Spo15|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1957
Score = 29.5 bits (63), Expect = 0.47
Identities = 23/96 (23%), Positives = 45/96 (46%)
Frame = +2
Query: 146 INKFARLNAKVDDYKDELKVKQNDVKNLEEAVEELSLADDSEKIPYLIGEIFICQNLEIT 325
++ F ++N K + + L + + +E+L D +LI + + L+ +
Sbjct: 1294 LDAFNQVNFKKMELDNRLTTTDAEFTKVVADLEKLQHEHDD----WLIQRGDLEKALKDS 1349
Query: 326 LKNLEEAKAKKIHEIHDLEEKCEELKSQMSDLKAHL 433
KN +A+ IH LEE EE K ++++L + L
Sbjct: 1350 EKNFLRKEAEMTENIHSLEEGKEETKKEIAELSSRL 1385
>SPAC6G9.06c |pcp1||pericentrin Pcp1|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1208
Score = 29.1 bits (62), Expect = 0.62
Identities = 13/47 (27%), Positives = 27/47 (57%)
Frame = +2
Query: 107 DSDVHISYEDQQKINKFARLNAKVDDYKDELKVKQNDVKNLEEAVEE 247
+ +V YE +Q N+ L+ ++D +D L K++++ L+ +EE
Sbjct: 278 NKEVDYEYEIRQLQNRLDELSEELDVAQDLLTEKEDEIATLKRQIEE 324
>SPBC29A10.13 |atp7||F0-ATPase subunit D|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 175
Score = 28.7 bits (61), Expect = 0.82
Identities = 12/32 (37%), Positives = 21/32 (65%)
Frame = +2
Query: 308 QNLEITLKNLEEAKAKKIHEIHDLEEKCEELK 403
QNL TLKN+E+A+ + I D+++ E++
Sbjct: 117 QNLSATLKNIEQARPTEEITIEDMKQAVPEIE 148
>SPAC7D4.04 |taf1||Taz1 interacting factor 1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 926
Score = 27.9 bits (59), Expect = 1.4
Identities = 19/95 (20%), Positives = 46/95 (48%), Gaps = 4/95 (4%)
Frame = +2
Query: 146 INKFARLNAKVDDYKDELKVKQNDVKNLEEAVEELSLADDSEKIPYLIGEIFICQNLEIT 325
+N ++ KVD ++ + + +L++ E +LA D+E + ++I E +
Sbjct: 641 LNLYSVFEGKVDSLQELYNAFKLQLTSLKQKGEYATLAKDAEAVQHIIEERDYALAEKAD 700
Query: 326 LKNLEEAKAKK----IHEIHDLEEKCEELKSQMSD 418
L L E + ++ +++ + +C EL+S + +
Sbjct: 701 LLKLSENRKEQCKILTQKLYTIVFRCNELQSVLRE 735
>SPCC63.11 |prp28||U5 snRNP-associated protein Prp28
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 27.9 bits (59), Expect = 1.4
Identities = 16/61 (26%), Positives = 34/61 (55%), Gaps = 1/61 (1%)
Frame = +2
Query: 209 QNDVKNLEEAVEELSLADDSEKIPYLIGEIFICQ-NLEITLKNLEEAKAKKIHEIHDLEE 385
Q+ + +LE+ V++ + ++ P + + + LE K +EEAKAK+ ++ DL +
Sbjct: 4 QDSIPSLEQLVQQKRVKEEKAARPKFLSKAERARLALERRQKEVEEAKAKQNDKLLDLRK 63
Query: 386 K 388
+
Sbjct: 64 R 64
>SPAC144.14 |klp8||kinesin-like protein Klp8|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 511
Score = 27.9 bits (59), Expect = 1.4
Identities = 16/28 (57%), Positives = 19/28 (67%), Gaps = 1/28 (3%)
Frame = +2
Query: 353 KKIHEI-HDLEEKCEELKSQMSDLKAHL 433
K I EI +D EE+ L+SQ S LKAHL
Sbjct: 402 KFIEEIRNDYEERIHALESQNSALKAHL 429
>SPBC365.05c |slu7||splicing factor Slu7|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 379
Score = 27.5 bits (58), Expect = 1.9
Identities = 15/34 (44%), Positives = 20/34 (58%), Gaps = 1/34 (2%)
Frame = +2
Query: 176 VDDYKDEL-KVKQNDVKNLEEAVEELSLADDSEK 274
+D Y D KVK KN E+ V +L++ D SEK
Sbjct: 325 LDRYGDGTSKVKDKKAKNNEKEVPDLAILDKSEK 358
>SPAC15A10.16 |bud6|aip3, fat1, SPAC15E1.01|actin interacting protein
3 homolog Bud6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1385
Score = 27.5 bits (58), Expect = 1.9
Identities = 18/73 (24%), Positives = 38/73 (52%), Gaps = 5/73 (6%)
Frame = +2
Query: 101 QPDSDVHISYEDQQKINKFARLNAKVDDYKDELKV-----KQNDVKNLEEAVEELSLADD 265
+PDS+ + ++ ++ + RL +VD++ E+K K N + EEA ++ D
Sbjct: 1166 KPDSEARLQAIERSELLQKKRLLQRVDEFSKEVKTFVENEKLNKIGGAEEADRIRTIQD- 1224
Query: 266 SEKIPYLIGEIFI 304
EK+ ++ + F+
Sbjct: 1225 -EKVRKILWDDFV 1236
>SPBC21D10.10 |||bromodomain protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 299
Score = 27.1 bits (57), Expect = 2.5
Identities = 23/82 (28%), Positives = 40/82 (48%), Gaps = 2/82 (2%)
Frame = +2
Query: 179 DDYKDELKVKQNDV-KNLEEAVEELSLADDSEKIPYLIGEIFICQN-LEITLKNLEEAKA 352
D K + K+ N V +N +A + L ++ + L EI C+ LE + + +AKA
Sbjct: 56 DSNKSDSKLLSNQVSQNDNDARKNLRKRLRNDVLKDLAEEIQGCEKKLESLYEEVAKAKA 115
Query: 353 KKIHEIHDLEEKCEELKSQMSD 418
K + + LEE +E K ++
Sbjct: 116 KAVEDQLALEEADKEAKKAKTE 137
>SPCC970.10c |brl2|rfp1|ubiquitin-protein ligase E3
Brl2|Schizosaccharomyces pombe|chr 3|||Manual
Length = 680
Score = 26.2 bits (55), Expect = 4.4
Identities = 11/28 (39%), Positives = 17/28 (60%)
Frame = +2
Query: 176 VDDYKDELKVKQNDVKNLEEAVEELSLA 259
++D ++ V QND K + A ELSL+
Sbjct: 343 INDLDHQISVMQNDEKRINNAKTELSLS 370
>SPBC1289.01c |chr4|cfh3, SPBC1539.11c|chitin synthase regulatory
factor |Schizosaccharomyces pombe|chr 2|||Manual
Length = 633
Score = 26.2 bits (55), Expect = 4.4
Identities = 24/106 (22%), Positives = 43/106 (40%)
Frame = +2
Query: 77 STTAKGTFQPDSDVHISYEDQQKINKFARLNAKVDDYKDELKVKQNDVKNLEEAVEELSL 256
S A P + H S + + N V D L V + + A E+L
Sbjct: 160 SNEAADVVFPHEEAHFSNHNPKPNNGSPLQKQVVADLPFPLPVSDEEQLDWIRANEDLVH 219
Query: 257 ADDSEKIPYLIGEIFICQNLEITLKNLEEAKAKKIHEIHDLEEKCE 394
+ D ++ L ++ + + L L+ +++ +HEI+ LE CE
Sbjct: 220 SQDIDEA--LEWAEYVLRFTQSHLPYLQTYESENLHEINYLESMCE 263
>SPAC27F1.02c |cdc8|fus4|tropomyosin|Schizosaccharomyces pombe|chr
1|||Manual
Length = 161
Score = 25.8 bits (54), Expect = 5.8
Identities = 26/104 (25%), Positives = 48/104 (46%), Gaps = 3/104 (2%)
Frame = +2
Query: 131 EDQQKINKFARLNAKVDDY-KDELKVKQNDVKNLEEAVE--ELSLADDSEKIPYLIGEIF 301
E+ ++ K RL A +D K E + V+ LEE +E + L + +EK+
Sbjct: 58 EELEEETKQLRLKADNEDIQKTEAEQLSRKVELLEEELETNDKLLRETTEKM-------- 109
Query: 302 ICQNLEITLKNLEEAKAKKIHEIHDLEEKCEELKSQMSDLKAHL 433
+ ++ ++ E E D+E+K EE+ + + +KA L
Sbjct: 110 --RQTDVKAEHFERRVQSLERERDDMEQKLEEMTDKYTKVKAEL 151
>SPBC29A3.13 |||PWWP domain protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 359
Score = 25.8 bits (54), Expect = 5.8
Identities = 12/42 (28%), Positives = 22/42 (52%)
Frame = +2
Query: 275 IPYLIGEIFICQNLEITLKNLEEAKAKKIHEIHDLEEKCEEL 400
IP+L E+ L LK + + + E++D+ +KC+ L
Sbjct: 301 IPFLNYELITSTKLAKVLKRIAFLEHLENDELYDIRQKCKNL 342
>SPBC543.09 |||mitochondrial m-AAA protease|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 773
Score = 25.4 bits (53), Expect = 7.6
Identities = 10/27 (37%), Positives = 20/27 (74%)
Frame = +2
Query: 170 AKVDDYKDELKVKQNDVKNLEEAVEEL 250
AK+ +++ ++K+K DV ++EA EE+
Sbjct: 282 AKMFNHETDIKIKFADVAGVDEAKEEI 308
>SPBC18E5.03c |sim4||kinetochore protein Sim4 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 277
Score = 25.4 bits (53), Expect = 7.6
Identities = 25/101 (24%), Positives = 43/101 (42%), Gaps = 6/101 (5%)
Frame = +2
Query: 134 DQQKINKFARLNAKVDDYKDELKVKQNDVKNLEEAVEELSLADDSEKIPYLIGEIFICQN 313
DQ + K + KV + + K ++ ++ LE E + L P +IG+ I N
Sbjct: 40 DQLLVEKIEKWEKKVSKFTENSKSEKRKLQ-LELFQEIIDLKKCIAFKP-IIGKNAIDNN 97
Query: 314 LEITLKNLEEAKA------KKIHEIHDLEEKCEELKSQMSD 418
+ KNL K +++H+I + LKS M +
Sbjct: 98 ISDLKKNLHSNKKLEAVLKEELHQIKKFSSDLQSLKSSMGE 138
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,242,599
Number of Sequences: 5004
Number of extensions: 39318
Number of successful extensions: 161
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 154
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 161
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 311890690
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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