BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmnc25m20
(305 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC1620.01c |lsm2||U6 snRNP-associated protein core protein|Sch... 86 1e-18
SPBC30D10.06 |lsm4||U6 snRNP-associated protein Lsm4|Schizosacch... 36 0.002
SPAC22F3.12c |rgs1||regulator of G-protein signaling Rgs1|Schizo... 26 1.1
SPAC22H10.05c |||mRNA cleavage and polyadenylation specificity f... 25 1.9
SPBC20F10.09 |lsm5||U6 snRNP-associated protein Lsm5|Schizosacch... 25 2.5
SPBC3E7.14 |smf1|SPBC4F6.01|Sm snRNP core protein Smf1|Schizosac... 25 3.3
SPAC23C4.12 |hhp2||serine/threonine protein kinase Hhp2 |Schizos... 24 5.8
SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces... 23 7.7
SPBC9B6.05c |lsm3||U6 snRNP-associated protein Lsm3|Schizosaccha... 23 7.7
SPCC5E4.07 |rpl2802|rpl28-2|60S ribosomal protein L27a.2/L28A|Sc... 23 7.7
SPCC1235.05c |fft2||fun thirty related protein Fft2|Schizosaccha... 23 7.7
SPBC776.11 |rpl2801|rpl28-1|60S ribosomal protein Ll28B 27a|Schi... 23 7.7
>SPCC1620.01c |lsm2||U6 snRNP-associated protein core
protein|Schizosaccharomyces pombe|chr 3|||Manual
Length = 96
Score = 85.8 bits (203), Expect = 1e-18
Identities = 41/71 (57%), Positives = 53/71 (74%)
Frame = +2
Query: 2 ICGTLHSVDQYLNIKLSDISVIDSEKYPHMLSVKNCFIRGSVVRYVQLPADEVDTQLLQD 181
I G L SVDQ+LN+KL +ISV+D+ KYPHM +VK+ FIRGSVVRYV + + VDT LL D
Sbjct: 25 IRGILKSVDQFLNVKLENISVVDASKYPHMAAVKDLFIRGSVVRYVHMSSAYVDTILLAD 84
Query: 182 AARKEATVSTR 214
A R++ + R
Sbjct: 85 ACRRDLANNKR 95
>SPBC30D10.06 |lsm4||U6 snRNP-associated protein
Lsm4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 121
Score = 35.5 bits (78), Expect = 0.002
Identities = 19/71 (26%), Positives = 42/71 (59%), Gaps = 2/71 (2%)
Frame = +2
Query: 8 GTLHSVDQYLNIKLSDI--SVIDSEKYPHMLSVKNCFIRGSVVRYVQLPADEVDTQLLQD 181
G L + D Y+N+ L ++ ++ D +K+ + C+IRG+ ++Y+++ DEV +Q+ +
Sbjct: 27 GHLENCDNYMNLTLREVIRTMPDGDKF---FRLPECYIRGNNIKYLRI-QDEVLSQVAKQ 82
Query: 182 AARKEATVSTR 214
A++ +R
Sbjct: 83 QAQQRENRGSR 93
>SPAC22F3.12c |rgs1||regulator of G-protein signaling
Rgs1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 481
Score = 26.2 bits (55), Expect = 1.1
Identities = 9/27 (33%), Positives = 14/27 (51%)
Frame = +2
Query: 74 EKYPHMLSVKNCFIRGSVVRYVQLPAD 154
E + H + NCF+ + V LP+D
Sbjct: 398 ESFAHACGIYNCFLSSNAPNAVNLPSD 424
>SPAC22H10.05c |||mRNA cleavage and polyadenylation specificity
factor complex subunit |Schizosaccharomyces pombe|chr
1|||Manual
Length = 456
Score = 25.4 bits (53), Expect = 1.9
Identities = 16/36 (44%), Positives = 19/36 (52%)
Frame = -3
Query: 183 ASWRSWVSTSSAGSCTYRTTEPLIKQFFTDNI*GYF 76
A+W S V SS+G C R E I+QF I YF
Sbjct: 280 ATWLSVVKVSSSGGCIDR-EEEWIQQFQARCIKQYF 314
>SPBC20F10.09 |lsm5||U6 snRNP-associated protein
Lsm5|Schizosaccharomyces pombe|chr 2|||Manual
Length = 80
Score = 25.0 bits (52), Expect = 2.5
Identities = 14/34 (41%), Positives = 19/34 (55%), Gaps = 5/34 (14%)
Frame = +2
Query: 8 GTLHSVDQYLNIKLSDISVID-----SEKYPHML 94
GTL D Y+NI L D++ D +EK+ ML
Sbjct: 31 GTLVGFDDYVNIVLKDVTEYDTVTGVTEKHSEML 64
>SPBC3E7.14 |smf1|SPBC4F6.01|Sm snRNP core protein
Smf1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 78
Score = 24.6 bits (51), Expect = 3.3
Identities = 9/14 (64%), Positives = 12/14 (85%)
Frame = +2
Query: 8 GTLHSVDQYLNIKL 49
GTL SVD Y+N++L
Sbjct: 32 GTLQSVDSYMNLQL 45
>SPAC23C4.12 |hhp2||serine/threonine protein kinase Hhp2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 400
Score = 23.8 bits (49), Expect = 5.8
Identities = 8/16 (50%), Positives = 14/16 (87%)
Frame = -2
Query: 67 NHAYIRKLYIEILIHR 20
N+AY+RKL+ ++LI +
Sbjct: 266 NYAYLRKLFRDLLIRK 281
>SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1485
Score = 23.4 bits (48), Expect = 7.7
Identities = 9/19 (47%), Positives = 11/19 (57%)
Frame = +1
Query: 1 DMWDVAFCGSISQYKAFGY 57
D WDVAF S Q+K +
Sbjct: 341 DRWDVAFAVSDGQFKQVSF 359
>SPBC9B6.05c |lsm3||U6 snRNP-associated protein
Lsm3|Schizosaccharomyces pombe|chr 2|||Manual
Length = 93
Score = 23.4 bits (48), Expect = 7.7
Identities = 10/28 (35%), Positives = 18/28 (64%), Gaps = 4/28 (14%)
Frame = +2
Query: 8 GTLHSVDQYLNIKLSD----ISVIDSEK 79
G LH+ D++LN+ L D +++ D E+
Sbjct: 34 GRLHAYDEHLNMVLGDAEEIVTIFDDEE 61
>SPCC5E4.07 |rpl2802|rpl28-2|60S ribosomal protein
L27a.2/L28A|Schizosaccharomyces pombe|chr 3|||Manual
Length = 148
Score = 23.4 bits (48), Expect = 7.7
Identities = 11/28 (39%), Positives = 15/28 (53%)
Frame = +1
Query: 115 KRLRGSIRTTAG**GRHPASPGCR*KGG 198
++LRG + G G+H PG R K G
Sbjct: 9 RKLRGHVSAGHGRIGKHRKHPGGRGKAG 36
>SPCC1235.05c |fft2||fun thirty related protein
Fft2|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1284
Score = 23.4 bits (48), Expect = 7.7
Identities = 8/28 (28%), Positives = 14/28 (50%)
Frame = +2
Query: 131 RYVQLPADEVDTQLLQDAARKEATVSTR 214
+Y+Q+P D L+ RK + S +
Sbjct: 345 KYIQMPNDSTQASLMPSYTRKTSNASKK 372
>SPBC776.11 |rpl2801|rpl28-1|60S ribosomal protein Ll28B
27a|Schizosaccharomyces pombe|chr 2|||Manual
Length = 148
Score = 23.4 bits (48), Expect = 7.7
Identities = 11/28 (39%), Positives = 15/28 (53%)
Frame = +1
Query: 115 KRLRGSIRTTAG**GRHPASPGCR*KGG 198
++LRG + G G+H PG R K G
Sbjct: 9 RKLRGHVSAGHGRIGKHRKHPGGRGKAG 36
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,195,128
Number of Sequences: 5004
Number of extensions: 20717
Number of successful extensions: 49
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 47
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 49
length of database: 2,362,478
effective HSP length: 63
effective length of database: 2,047,226
effective search space used: 77794588
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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