BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmnc25k12
(706 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC28E12.05 |esf2|SPBC3H7.17c|U3 snoRNP-associated protein Esf2... 28 1.5
SPAC4F10.03c |||2'-O-ribose methyltransferase|Schizosaccharomyce... 27 2.0
SPBC29A3.09c |||AAA family ATPase Gcn20 |Schizosaccharomyces pom... 27 2.6
SPAC29B12.01 |ino80|SPAC3G6.12|SNF2 family helicase Ino80|Schizo... 27 2.6
SPAC1687.11 |spb1||rRNA methyltransferase Spb1 |Schizosaccharomy... 27 3.5
SPBC577.15c |||NASP family histone binding protein|Schizosacchar... 27 3.5
SPCC4B3.03c |||DUF21 domain protein|Schizosaccharomyces pombe|ch... 26 4.6
SPAC1805.09c |fmt1||methionyl-tRNA formyltransferase Fmt1 |Schiz... 26 4.6
SPCC622.10c |||exocyst complex subunit Sec5 |Schizosaccharomyces... 26 4.6
SPAC26A3.12c |dhp1||5'-3' exoribonuclease Dhp1 |Schizosaccharomy... 26 4.6
SPAC11E3.02c |||C2 domain protein|Schizosaccharomyces pombe|chr ... 26 6.0
SPAP27G11.05c |vps41||vacuolar protein sorting-associated protei... 26 6.0
SPAC1093.03 |||inositol polyphosphate phosphatase |Schizosacchar... 26 6.0
>SPBC28E12.05 |esf2|SPBC3H7.17c|U3 snoRNP-associated protein Esf2
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 334
Score = 27.9 bits (59), Expect = 1.5
Identities = 15/55 (27%), Positives = 29/55 (52%)
Frame = -1
Query: 229 IFKIGFLKHCSKRVVKRCVVGHIYNQFPPR*RTVLWRHFDFVAIQYLPSYRFPHI 65
+++ G+++ SKRV K V + N + W H D ++YLP +++ H+
Sbjct: 174 MYEEGWIEFESKRVAKS--VAELLNTNQIGGKKSSWYHDDIWNMKYLPKFKWHHL 226
>SPAC4F10.03c |||2'-O-ribose methyltransferase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 285
Score = 27.5 bits (58), Expect = 2.0
Identities = 11/35 (31%), Positives = 21/35 (60%)
Frame = +3
Query: 555 RPRCWRKLLEIDKKFHVCRHVDTFLDLCGGPGEFA 659
R R KLL+++++F++ +DLC PG ++
Sbjct: 22 RARSAFKLLQLNEQFNLFEGAKRVVDLCAAPGSWS 56
>SPBC29A3.09c |||AAA family ATPase Gcn20 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 736
Score = 27.1 bits (57), Expect = 2.6
Identities = 10/28 (35%), Positives = 17/28 (60%)
Frame = +3
Query: 549 TRRPRCWRKLLEIDKKFHVCRHVDTFLD 632
TR RC +L E +K+ +H+ +F+D
Sbjct: 442 TREERCKNQLREYEKQMEYRKHLQSFID 469
>SPAC29B12.01 |ino80|SPAC3G6.12|SNF2 family helicase
Ino80|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1604
Score = 27.1 bits (57), Expect = 2.6
Identities = 14/57 (24%), Positives = 26/57 (45%), Gaps = 6/57 (10%)
Frame = +2
Query: 71 WESIRWQILNGDEIEVSPEHRSLAWRELIINVANN------TPLDNTFRTMFQKADF 223
++S++WQ + DE + S W+ L+ N TP+ NT + ++ F
Sbjct: 966 FQSVKWQYMILDEAQAIKSSSSSRWKSLLAFKCRNRLLLTGTPIQNTMQELWALLHF 1022
>SPAC1687.11 |spb1||rRNA methyltransferase Spb1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 802
Score = 26.6 bits (56), Expect = 3.5
Identities = 10/32 (31%), Positives = 17/32 (53%)
Frame = +3
Query: 555 RPRCWRKLLEIDKKFHVCRHVDTFLDLCGGPG 650
R R KL+++++K+ +DLC PG
Sbjct: 26 RSRAAFKLVQLNQKYSFLEKAKVIIDLCAAPG 57
>SPBC577.15c |||NASP family histone binding
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 396
Score = 26.6 bits (56), Expect = 3.5
Identities = 11/32 (34%), Positives = 17/32 (53%)
Frame = +2
Query: 134 SLAWRELIINVANNTPLDNTFRTMFQKADFEN 229
+L W+E + NV+NNT L + +F N
Sbjct: 225 ALEWKEKVYNVSNNTLLSEAHYKLALALEFTN 256
>SPCC4B3.03c |||DUF21 domain protein|Schizosaccharomyces pombe|chr
3|||Manual
Length = 679
Score = 26.2 bits (55), Expect = 4.6
Identities = 11/25 (44%), Positives = 16/25 (64%)
Frame = -1
Query: 688 QSGFNDMV*LANSPGPPHRSKNVST 614
Q G + M+ ++NSPG PH + V T
Sbjct: 347 QEGKSHMILISNSPGEPHGAIGVIT 371
>SPAC1805.09c |fmt1||methionyl-tRNA formyltransferase Fmt1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 340
Score = 26.2 bits (55), Expect = 4.6
Identities = 13/35 (37%), Positives = 22/35 (62%)
Frame = -2
Query: 384 IQIKNKNT*AILTLVV*SLNRAGLLRAALILSLYI 280
+++ K T +LT ++ SL AG+L L+ SLY+
Sbjct: 155 LKLNGKETYTLLTKIL-SLGAAGMLEHVLLQSLYL 188
>SPCC622.10c |||exocyst complex subunit Sec5 |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 815
Score = 26.2 bits (55), Expect = 4.6
Identities = 14/32 (43%), Positives = 20/32 (62%), Gaps = 1/32 (3%)
Frame = +2
Query: 269 KTLTMYNERIRAALNRPARFNDQTTN-VNIAY 361
KT+ M E I AL + FN ++TN V+I+Y
Sbjct: 178 KTMDMCEESISGALQQLQLFNTESTNEVDISY 209
>SPAC26A3.12c |dhp1||5'-3' exoribonuclease Dhp1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 991
Score = 26.2 bits (55), Expect = 4.6
Identities = 12/50 (24%), Positives = 27/50 (54%), Gaps = 1/50 (2%)
Frame = +2
Query: 149 ELIIN-VANNTPLDNTFRTMFQKADFENFDYNTPIVYNLKTKTLTMYNER 295
+L+ N N PL+++ + D ++ + +TP+V+ + TK + +R
Sbjct: 520 QLVSNETLQNVPLEDSIASSSAYEDTDSIESSTPVVHPIDTKVSNVGQKR 569
>SPAC11E3.02c |||C2 domain protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1237
Score = 25.8 bits (54), Expect = 6.0
Identities = 10/19 (52%), Positives = 14/19 (73%)
Frame = -1
Query: 571 RQHLGLRVGRLSNNFERAQ 515
R+H+G VGR ++ ERAQ
Sbjct: 924 REHIGFYVGRTYHDLERAQ 942
>SPAP27G11.05c |vps41||vacuolar protein sorting-associated protein
Vps41|Schizosaccharomyces pombe|chr 1|||Manual
Length = 886
Score = 25.8 bits (54), Expect = 6.0
Identities = 18/66 (27%), Positives = 29/66 (43%)
Frame = +2
Query: 74 ESIRWQILNGDEIEVSPEHRSLAWRELIINVANNTPLDNTFRTMFQKADFENFDYNTPIV 253
ESI I ++ + E R I + N LDN F + + N+ P++
Sbjct: 771 ESISMAIKYREQEQSGLEFLVKGKRSKNIFLTNLVKLDNPFNSFGDEQLDYNYRKRIPMI 830
Query: 254 YNLKTK 271
Y+L+TK
Sbjct: 831 YDLRTK 836
>SPAC1093.03 |||inositol polyphosphate phosphatase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 832
Score = 25.8 bits (54), Expect = 6.0
Identities = 16/58 (27%), Positives = 30/58 (51%), Gaps = 3/58 (5%)
Frame = +2
Query: 185 DN-TFRTMFQKADFENF-DYNTPIVYNLKTKTLTMYNERIRAALN-RPARFNDQTTNV 349
DN T + +A + N+ DY+ + +K K L YN+ +A++ P +D++ V
Sbjct: 762 DNYTLLSKHDRAMYNNYADYSPDKIKEIKEKELNTYNDYFNSAISENPTLKSDRSEKV 819
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,969,273
Number of Sequences: 5004
Number of extensions: 60598
Number of successful extensions: 197
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 191
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 197
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 327172622
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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