Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmnc25j03
(670 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC11G7.03 |idh1|glu3|isocitrate dehydrogenase |Schizosaccharom... 133 2e-32
SPBC902.05c |idh2|glu2|isocitrate dehydrogenase |Schizosaccharom... 111 1e-25
SPAC31G5.04 |||homoisocitrate dehydrogenase|Schizosaccharomyces ... 42 6e-05
SPBC1A4.02c |leu1|SPBC1E8.07c|3-isopropylmalate dehydrogenase Le... 30 0.26
SPAC19G12.02c |pms1||MutL family mismatch-repair protein Pms1|Sc... 29 0.61
SPBPJ4664.06 |gpt1||UDP-glucose-glycoprotein glucosyltransferase... 28 1.1
SPBC2G5.05 |||transketolase |Schizosaccharomyces pombe|chr 2|||M... 28 1.1
SPBC3E7.15c |mug83|SPBC4F6.02c|sphingosine N-acyltransferase Lac... 27 1.8
SPAC1F7.01c |spt6|SPAC694.07c|transcription elongation factor Sp... 27 3.2
SPCC622.13c |||conserved eukaryotic protein|Schizosaccharomyces ... 26 4.3
SPBP19A11.04c |mor2|cps12|morphogenesis protein Mor2|Schizosacch... 26 5.6
SPAC1071.02 |||TFIIH regulator |Schizosaccharomyces pombe|chr 1|... 25 9.9
SPCC550.15c |||ribosome biogenesis protein |Schizosaccharomyces ... 25 9.9
>SPAC11G7.03 |idh1|glu3|isocitrate dehydrogenase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 356
Score = 133 bits (322), Expect = 2e-32
Identities = 69/144 (47%), Positives = 94/144 (65%), Gaps = 3/144 (2%)
Frame = +2
Query: 248 GRIKCTLIPGDGVGPELVYAVQEVFKAASIPVDFESFFFSEV---NPTLSAPLEDVVNSI 418
G+ TLIPGDG+G E AV E+FK A++P++FE + + N + L + + S+
Sbjct: 19 GKYTVTLIPGDGIGRETSNAVTEIFKTANVPIEFEEIDVTGMEKNNKSSGDALHEAIQSL 78
Query: 419 AVNKICIKGILATPDFSHTGELQTLNMKLRNALDLYANVVHVKSLPNVKCRHQDVDCIII 598
NK+ +KGIL TP F G + N+ LR LD+YA++V +K++P K RH +VD II
Sbjct: 79 KRNKVGLKGILFTP-FEKGGHT-SFNVALRKELDIYASLVLIKNIPGFKTRHDNVDFAII 136
Query: 599 REQTEGEYSALEHESVPGVVECLK 670
RE TEGEYS LEH+SVPGVVE LK
Sbjct: 137 RENTEGEYSGLEHQSVPGVVESLK 160
>SPBC902.05c |idh2|glu2|isocitrate dehydrogenase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 378
Score = 111 bits (266), Expect = 1e-25
Identities = 59/156 (37%), Positives = 91/156 (58%), Gaps = 2/156 (1%)
Frame = +2
Query: 209 APFGALQPRATKEGRIKCTLIPGDGVGPELVYAVQEVFKAASIPVDFESFFFSEV--NPT 382
A G + G T+I GDG+GPE+ +V+ +FKAA +P+++E + N T
Sbjct: 31 AAAGTYEGVKNANGNYTVTMIAGDGIGPEIAQSVERIFKAAKVPIEWERVKVYPILKNGT 90
Query: 383 LSAPLEDVVNSIAVNKICIKGILATPDFSHTGELQTLNMKLRNALDLYANVVHVKSLPNV 562
+ P +D S+ NK+ +KG LATP G + ++N+ LR L+ANV S+
Sbjct: 91 TTIP-DDAKESVRKNKVALKGPLATP--IGKGHV-SMNLTLRRTFGLFANVRPCVSITGY 146
Query: 563 KCRHQDVDCIIIREQTEGEYSALEHESVPGVVECLK 670
K + +V+ ++IRE TEGEYS +EHE +PGVV+ +K
Sbjct: 147 KTPYDNVNTVLIRENTEGEYSGIEHEVIPGVVQSIK 182
>SPAC31G5.04 |||homoisocitrate dehydrogenase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 362
Score = 42.3 bits (95), Expect = 6e-05
Identities = 44/145 (30%), Positives = 67/145 (46%), Gaps = 11/145 (7%)
Frame = +2
Query: 251 RIKCTLIPGDGVGPELVYAVQEVFKAASIPVDFE-SFFFSEVNP-------TLSAPLEDV 406
RI LIP DG+G E+V A + + + ++P + F F +++ T A E
Sbjct: 6 RIVLGLIPADGIGKEVVPAARRLME--NLPAKHKLKFDFIDLDAGWGTFERTGKALPERT 63
Query: 407 VNSIAVN-KICIKGILATPDFSHTGELQTLNMKLRNALDLYANVVHVKSLPNVKCRHQDV 583
V + + G + +P G + + LR + LYANV VKSL K + V
Sbjct: 64 VERLKTECNAALFGAVQSPTHKVAGYSSPI-VALRKKMGLYANVRPVKSLDGAK--GKPV 120
Query: 584 DCIIIREQTEGEYSALEH--ESVPG 652
D +I+RE TE Y E ++ PG
Sbjct: 121 DLVIVRENTECLYVKEERMVQNTPG 145
>SPBC1A4.02c |leu1|SPBC1E8.07c|3-isopropylmalate dehydrogenase
Leu1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 371
Score = 30.3 bits (65), Expect = 0.26
Identities = 24/101 (23%), Positives = 47/101 (46%), Gaps = 8/101 (7%)
Frame = +2
Query: 257 KCTLIPGDGVGPELVYAVQEVFKAA-----SIPVDFESFFFSEVN-PTLSAPLED-VVNS 415
K ++PGD +GPE+V + EV K + ++FE + PL D V +
Sbjct: 5 KIVVLPGDHIGPEIVASALEVLKVVEKKRPELKLEFEEHKIGGASIDAYGTPLTDETVKA 64
Query: 416 IAVNKICIKGILATPDFSHTG-ELQTLNMKLRNALDLYANV 535
+ G + P++++ + +KLR ++ ++AN+
Sbjct: 65 CLEADGVLLGAVGGPEWTNPNCRPEQGLLKLRKSMGVWANL 105
>SPAC19G12.02c |pms1||MutL family mismatch-repair protein
Pms1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 794
Score = 29.1 bits (62), Expect = 0.61
Identities = 25/83 (30%), Positives = 39/83 (46%)
Frame = +2
Query: 290 PELVYAVQEVFKAASIPVDFESFFFSEVNPTLSAPLEDVVNSIAVNKICIKGILATPDFS 469
P A+Q++ S P+D + F ++N +LS +D+V S A+ K K I D S
Sbjct: 533 PSKTAALQKLKFFQSRPLDGLNKFSKKINISLSGVQKDIVRSDALLKFSNK-IGVVHDIS 591
Query: 470 HTGELQTLNMKLRNALDLYANVV 538
+ LN+ + A L VV
Sbjct: 592 DENQEDHLNLTVHKADFLRMRVV 614
>SPBPJ4664.06 |gpt1||UDP-glucose-glycoprotein glucosyltransferase
Gpt1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1448
Score = 28.3 bits (60), Expect = 1.1
Identities = 16/70 (22%), Positives = 31/70 (44%)
Frame = +2
Query: 338 PVDFESFFFSEVNPTLSAPLEDVVNSIAVNKICIKGILATPDFSHTGELQTLNMKLRNAL 517
P F S N L D + ++ +KI I +LAT + + ++ T +L
Sbjct: 245 PASFTSSRNKRSNERLFGMTSDSLQTVTPDKIAILDLLATQSIASSTDMLTAFRELTQDF 304
Query: 518 DLYANVVHVK 547
+YA+ + ++
Sbjct: 305 PIYAHYLSIQ 314
>SPBC2G5.05 |||transketolase |Schizosaccharomyces pombe|chr
2|||Manual
Length = 685
Score = 28.3 bits (60), Expect = 1.1
Identities = 11/19 (57%), Positives = 13/19 (68%)
Frame = -3
Query: 221 LQMVHNIHFSPLLPNLCVH 165
LQ H++H SPL P CVH
Sbjct: 261 LQGTHSVHGSPLKPEDCVH 279
>SPBC3E7.15c |mug83|SPBC4F6.02c|sphingosine N-acyltransferase
Lac1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 384
Score = 27.5 bits (58), Expect = 1.8
Identities = 12/32 (37%), Positives = 16/32 (50%)
Frame = -1
Query: 433 NLVNSDRVYDVFEWCTKCWIHFRKEKGFKVHW 338
N VN+ VY +F WI+ R FK+ W
Sbjct: 269 NYVNTVIVYPIFVIFVFVWIYMRHYLNFKIMW 300
>SPAC1F7.01c |spt6|SPAC694.07c|transcription elongation factor
Spt6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1365
Score = 26.6 bits (56), Expect = 3.2
Identities = 11/37 (29%), Positives = 24/37 (64%), Gaps = 1/37 (2%)
Frame = -1
Query: 436 TNLVNSDRVYDVFEWCTKCW-IHFRKEKGFKVHWNAG 329
T L++ + ++++F CTK W +H +K+ K++ + G
Sbjct: 355 TPLLSQNDLWNIFFLCTKFWSLHSKKQDILKLYSDLG 391
>SPCC622.13c |||conserved eukaryotic protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1098
Score = 26.2 bits (55), Expect = 4.3
Identities = 16/54 (29%), Positives = 24/54 (44%)
Frame = +2
Query: 365 SEVNPTLSAPLEDVVNSIAVNKICIKGILATPDFSHTGELQTLNMKLRNALDLY 526
S++N A LED N +C++ +A S LQ +K R+ L Y
Sbjct: 541 SQLNVFTKASLEDKQKKEKYNLLCVRSCIAIDSISWISSLQ--GVKFRSKLMAY 592
>SPBP19A11.04c |mor2|cps12|morphogenesis protein
Mor2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 2196
Score = 25.8 bits (54), Expect = 5.6
Identities = 14/44 (31%), Positives = 25/44 (56%)
Frame = -1
Query: 247 FLCCPRLQGSKWCITYIFLRCYRTCVYTLSNMLRSLHNSSKNIP 116
FL P +Q + Y+ LRCY + + ++++LH SS+ +P
Sbjct: 1000 FLSTPNVQADE---KYLKLRCYFSQLLEKVLLVQNLHPSSEVLP 1040
>SPAC1071.02 |||TFIIH regulator |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1018
Score = 25.0 bits (52), Expect = 9.9
Identities = 14/47 (29%), Positives = 24/47 (51%)
Frame = +2
Query: 377 PTLSAPLEDVVNSIAVNKICIKGILATPDFSHTGELQTLNMKLRNAL 517
P+L A +D+ N V + +K + + P+F+ T LQ K+ L
Sbjct: 948 PSLLAIPKDMNNPTVVRLLALKCLGSLPEFTPTTNLQLFRDKVIRGL 994
>SPCC550.15c |||ribosome biogenesis protein |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 463
Score = 25.0 bits (52), Expect = 9.9
Identities = 7/20 (35%), Positives = 11/20 (55%)
Frame = -1
Query: 391 CTKCWIHFRKEKGFKVHWNA 332
CT C + F + K+HW +
Sbjct: 7 CTTCTVAFNNAESQKIHWKS 26
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,850,059
Number of Sequences: 5004
Number of extensions: 61444
Number of successful extensions: 184
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 177
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 179
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 305854096
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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