Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmnc25f14
(671 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC2F12.04 |rpl1701|rpl17, rpl17-1|60S ribosomal protein L17|Sc... 198 8e-52
SPCC364.03 |rpl1702|rpl17-2, rpl17|60S ribosomal protein L17|Sch... 196 2e-51
SPAC630.06c |||conserved fungal protein|Schizosaccharomyces pomb... 27 1.9
SPAC13F5.01c |msh1|SPAC23C11.18c|MutS protein homolog 1|Schizosa... 27 1.9
SPBC23G7.04c |nif1||SEL1 repear protein Nif1|Schizosaccharomyces... 27 2.5
SPBC19C7.02 |ubr1|SPBC32F12.14|N-end-recognizing protein Ubr1|Sc... 27 3.3
SPCC970.10c |brl2|rfp1|ubiquitin-protein ligase E3 Brl2|Schizosa... 25 9.9
SPBC32H8.02c |nep2|mug120|nedd8 protease Nep2|Schizosaccharomyce... 25 9.9
SPAC17C9.11c |||zinc finger protein, zf-C2H2 type/UBA domain pro... 25 9.9
>SPBC2F12.04 |rpl1701|rpl17, rpl17-1|60S ribosomal protein
L17|Schizosaccharomyces pombe|chr 2|||Manual
Length = 187
Score = 198 bits (482), Expect = 8e-52
Identities = 92/163 (56%), Positives = 115/163 (70%)
Frame = +2
Query: 104 MGRYSREPDNPAKSCKARGSNLRVHFKNTYETAMAIRKMPLRRAVRYLKNVIEKKECIPF 283
M RYS P K KARG+ LR HFKN+ E A I M L++A +L NV E K+ +PF
Sbjct: 1 MVRYSASPALETKCAKARGAYLRTHFKNSREVAFTINGMSLKKAFIFLDNVKEHKQAVPF 60
Query: 284 RRFNGGVGRCAQAKQFGTTQGRWPKKSAEFLLQLLRNAESNADNKTLDVDRLVIDHIQVN 463
RRFNGGVGR AQ K+FG TQ RWP KS +F LL+NAE+NA+ K LD+D+L+I H+QVN
Sbjct: 61 RRFNGGVGRTAQGKEFGVTQARWPVKSVKFFYDLLKNAEANAEAKGLDMDKLIIKHVQVN 120
Query: 464 RAPCLRRRTYRAHGRINPYMSSPCHIEVCLSEREDAVARVAPT 592
AP RRRTYRAHGR+ Y+SSP HIE+ ++E E+AV + T
Sbjct: 121 AAPKQRRRTYRAHGRVTAYLSSPSHIEIIVAEEEEAVPKANDT 163
>SPCC364.03 |rpl1702|rpl17-2, rpl17|60S ribosomal protein
L17|Schizosaccharomyces pombe|chr 3|||Manual
Length = 187
Score = 196 bits (479), Expect = 2e-51
Identities = 92/163 (56%), Positives = 114/163 (69%)
Frame = +2
Query: 104 MGRYSREPDNPAKSCKARGSNLRVHFKNTYETAMAIRKMPLRRAVRYLKNVIEKKECIPF 283
M RYS P K KARG+ LR HFKN+ E A I M L++A +L NV E K+ +PF
Sbjct: 1 MVRYSAAPALETKCAKARGAYLRTHFKNSREVAFTINGMNLKKAFIFLDNVKEHKQAVPF 60
Query: 284 RRFNGGVGRCAQAKQFGTTQGRWPKKSAEFLLQLLRNAESNADNKTLDVDRLVIDHIQVN 463
RRFNGGVGR AQ K+FG TQ RWP KS F LL+NAE+NA+ K LD+D+L+I H+QVN
Sbjct: 61 RRFNGGVGRTAQGKEFGVTQARWPVKSVNFFYDLLKNAEANAEAKGLDMDKLIIKHVQVN 120
Query: 464 RAPCLRRRTYRAHGRINPYMSSPCHIEVCLSEREDAVARVAPT 592
AP RRRTYRAHGR+ Y+SSP HIE+ ++E E+AV + T
Sbjct: 121 AAPKQRRRTYRAHGRVTAYLSSPSHIEIIVAEEEEAVPKANDT 163
>SPAC630.06c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 188
Score = 27.5 bits (58), Expect = 1.9
Identities = 23/82 (28%), Positives = 36/82 (43%)
Frame = +2
Query: 8 FSYFLLRVRISAVYPRCFCVYLVLSVKWHEIIMGRYSREPDNPAKSCKARGSNLRVHFKN 187
F+Y L +P C ++ V+S+K E S+ P+K + R LR +
Sbjct: 109 FTYDQLMKAKEEKWPACQKLHKVVSIKKEE------SKHRRRPSKKRRIRMKLLREKEEQ 162
Query: 188 TYETAMAIRKMPLRRAVRYLKN 253
AMA ++ R+ VR L N
Sbjct: 163 LKRQAMAAKRNRFRKNVRKLPN 184
>SPAC13F5.01c |msh1|SPAC23C11.18c|MutS protein homolog
1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 941
Score = 27.5 bits (58), Expect = 1.9
Identities = 14/39 (35%), Positives = 24/39 (61%), Gaps = 1/39 (2%)
Frame = +2
Query: 164 NLRVHFKNTYETAMAIRKMPLRRAVRYLKNVIEK-KECI 277
NLRV K T ++ +++ P + RYLK ++E K+C+
Sbjct: 102 NLRVSKKKTSKSDVSMAGFPFFKLDRYLKILVEDLKKCV 140
>SPBC23G7.04c |nif1||SEL1 repear protein Nif1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 681
Score = 27.1 bits (57), Expect = 2.5
Identities = 14/36 (38%), Positives = 20/36 (55%), Gaps = 3/36 (8%)
Frame = +1
Query: 454 SGKSRALPTQTYIPCSRS---HQPLHVVSLPHRSMS 552
S K RA +T + ++S HQ +H +S PH S S
Sbjct: 340 SDKERANLNKTMVSINKSINIHQSIHEISCPHHSSS 375
>SPBC19C7.02 |ubr1|SPBC32F12.14|N-end-recognizing protein
Ubr1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1958
Score = 26.6 bits (56), Expect = 3.3
Identities = 11/28 (39%), Positives = 18/28 (64%)
Frame = -1
Query: 341 VLCQTALLEHSDQRRR*SDGMEYTLSFQ 258
++C TA+L+H D RR G+ + L F+
Sbjct: 846 IVCNTAVLDHWDITRRIEYGIAHILCFR 873
>SPCC970.10c |brl2|rfp1|ubiquitin-protein ligase E3
Brl2|Schizosaccharomyces pombe|chr 3|||Manual
Length = 680
Score = 25.0 bits (52), Expect = 9.9
Identities = 17/49 (34%), Positives = 25/49 (51%), Gaps = 1/49 (2%)
Frame = +1
Query: 517 LH-VVSLPHRSMSQRTRRCCRQGSPY**CPSKEKAFQEKACASKGEDDE 660
LH ++ LP RS S T +C + P K +E +SKG+D+E
Sbjct: 182 LHSIIKLPDRS-SSFTLQCINESVR----PQSTKVKEEATTSSKGKDEE 225
>SPBC32H8.02c |nep2|mug120|nedd8 protease Nep2|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 415
Score = 25.0 bits (52), Expect = 9.9
Identities = 11/31 (35%), Positives = 16/31 (51%), Gaps = 1/31 (3%)
Frame = -2
Query: 277 NTLFLFNHVFEVTNSTTERHLSDCHCGL-IC 188
N L F V + T + ++DC CGL +C
Sbjct: 204 NLGILLKKEFRVRHMKTPQQINDCDCGLHVC 234
>SPAC17C9.11c |||zinc finger protein, zf-C2H2 type/UBA domain
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 240
Score = 25.0 bits (52), Expect = 9.9
Identities = 12/36 (33%), Positives = 23/36 (63%)
Frame = +2
Query: 161 SNLRVHFKNTYETAMAIRKMPLRRAVRYLKNVIEKK 268
+N ++ K+ ETA A+RKM + +R L+ + ++K
Sbjct: 73 TNYKILQKSNDETAQAMRKMQDQARLRDLQKIRQQK 108
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,715,559
Number of Sequences: 5004
Number of extensions: 55190
Number of successful extensions: 144
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 142
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 144
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 307866294
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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