Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmnc25f02
(611 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC26F1.09 |gyp51||GTPase activating protein Gyp51 |Schizosacch... 29 0.40
SPBC1604.20c |tea2|klp4|kinesin-like protein Tea2|Schizosaccharo... 28 0.93
SPAC8C9.15c |tif225||translation initiation factor eIF2B epsilon... 28 1.2
SPBC28E12.05 |esf2|SPBC3H7.17c|U3 snoRNP-associated protein Esf2... 28 1.2
SPAC1093.06c |dhc1|SPAC30C2.01c|dynein heavy chain |Schizosaccha... 28 1.2
SPAP8A3.14c |||mitochondrial inner membrane protein |Schizosacch... 27 2.1
SPBC16H5.05c |cyp7|cwf27|cyclophilin family peptidyl-prolyl cis-... 27 2.1
SPAC29B12.01 |ino80|SPAC3G6.12|SNF2 family helicase Ino80|Schizo... 27 2.1
SPCC1020.01c |pma2|SPCC1393.01|P-type proton ATPase Pma2 |Schizo... 27 2.8
SPBC577.15c |||NASP family histone binding protein|Schizosacchar... 27 2.8
SPAC26A3.12c |dhp1||5'-3' exoribonuclease Dhp1 |Schizosaccharomy... 26 3.7
SPAP27G11.05c |vps41||vacuolar protein sorting-associated protei... 26 4.9
SPCC1494.10 ||SPCC70.01|transcription factor |Schizosaccharomyce... 25 6.5
SPAC1071.10c |pma1||P-type proton ATPase Pma1 |Schizosaccharomyc... 25 6.5
SPAPB1E7.07 |glt1||glutamate synthase Glt1 |Schizosaccharomyces ... 25 8.6
SPAC22F3.10c |gcs1|apd1|glutamate-cysteine ligase Gcs1 |Schizosa... 25 8.6
SPBC16H5.13 |||WD repeat protein |Schizosaccharomyces pombe|chr ... 25 8.6
SPAC589.12 ||SPAC688.01|glycosylceramide biosynthesis protein |S... 25 8.6
>SPAC26F1.09 |gyp51||GTPase activating protein Gyp51
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1031
Score = 29.5 bits (63), Expect = 0.40
Identities = 15/27 (55%), Positives = 20/27 (74%)
Frame = +2
Query: 515 SFKDMEEGKCYDISLNYVKTKFSQMIQ 595
+FK+ +EG+ YD SL +VKT FS IQ
Sbjct: 844 AFKETQEGENYDTSL-FVKTAFSFEIQ 869
>SPBC1604.20c |tea2|klp4|kinesin-like protein
Tea2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 628
Score = 28.3 bits (60), Expect = 0.93
Identities = 27/99 (27%), Positives = 42/99 (42%), Gaps = 1/99 (1%)
Frame = -2
Query: 412 TINSFVIFSNLLGAISHSHAFYKRLTATFSRKKSFGRH-IAVVVNMWESIRWQILNGDEI 236
TI +F+N+ G S ++ YKR + R G + I M + + + G E
Sbjct: 174 TIQQEYLFNNVFGMESKNYDIYKRSVKSVVRNVFSGYNGIVFAYGMTGTGKTYSMQGTEN 233
Query: 235 EVSPEHRSLAWRELIINVANNTPLDNTFRTMFQKADFEN 119
E P LA +L V NN+ D+TF+ + N
Sbjct: 234 E--PGIIPLAMNDLFEMVENNSD-DDTFQIRISYLEIYN 269
>SPAC8C9.15c |tif225||translation initiation factor eIF2B epsilon
subunit|Schizosaccharomyces pombe|chr 1|||Manual
Length = 678
Score = 27.9 bits (59), Expect = 1.2
Identities = 10/22 (45%), Positives = 14/22 (63%)
Frame = +2
Query: 452 YYYTFRIMSDNKIQEYYGDSQS 517
Y+Y I +N IQE+Y D +S
Sbjct: 623 YFYQLEIAEENAIQEWYSDPRS 644
>SPBC28E12.05 |esf2|SPBC3H7.17c|U3 snoRNP-associated protein Esf2
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 334
Score = 27.9 bits (59), Expect = 1.2
Identities = 15/55 (27%), Positives = 29/55 (52%)
Frame = +2
Query: 119 IFKIGFLKHCSKRVVKRCVVGHIYNQFPPR*RTVLWRHFDFVAIQYLPSYRFPHI 283
+++ G+++ SKRV K V + N + W H D ++YLP +++ H+
Sbjct: 174 MYEEGWIEFESKRVAKS--VAELLNTNQIGGKKSSWYHDDIWNMKYLPKFKWHHL 226
>SPAC1093.06c |dhc1|SPAC30C2.01c|dynein heavy chain
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 4196
Score = 27.9 bits (59), Expect = 1.2
Identities = 20/54 (37%), Positives = 29/54 (53%), Gaps = 1/54 (1%)
Frame = -2
Query: 445 IDTQRHFAH*FTI-NSFVIFSNLLGAISHSHAFYKRLTATFSRKKSFGRHIAVV 287
ID Q H + T+ NS V+ +NL + H+F+K + FS K GR+ VV
Sbjct: 1435 IDLQAHESFITTLLNSAVVEANLENQFNEVHSFWKN--SYFSFKSFKGRNYIVV 1486
>SPAP8A3.14c |||mitochondrial inner membrane protein
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 677
Score = 27.1 bits (57), Expect = 2.1
Identities = 11/41 (26%), Positives = 24/41 (58%)
Frame = -2
Query: 406 NSFVIFSNLLGAISHSHAFYKRLTATFSRKKSFGRHIAVVV 284
+S++ +S++ +SHSH + R+ + K+F I+ +V
Sbjct: 621 DSYITYSDIFSPLSHSHKDWFRIHTEENSSKNFYEIISEIV 661
>SPBC16H5.05c |cyp7|cwf27|cyclophilin family peptidyl-prolyl
cis-trans isomerase Cyp7|Schizosaccharomyces pombe|chr
2|||Manual
Length = 463
Score = 27.1 bits (57), Expect = 2.1
Identities = 27/100 (27%), Positives = 45/100 (45%), Gaps = 6/100 (6%)
Frame = +2
Query: 323 GESSGEPLIKRMAMANSPKKIRENYKRINGKLMGKMTLSIDNEYYYTFRIMSD------N 484
G S+ +P++ R N + E YK+ ++GK +++N+ T R +S N
Sbjct: 314 GSSNSKPVVVRPKKRNILTEELEKYKKSKKVVLGKRK-NLENDEESTLRALSSFQSKIRN 372
Query: 485 KIQEYYGDSQSFKDMEEGKCYDISLNYVKTKFSQMIQINE 604
E DSQ +E+ C SL+ V FS ++ E
Sbjct: 373 AEDEDVMDSQYGSKIEDTPC---SLHNVPGCFSCFDRLGE 409
>SPAC29B12.01 |ino80|SPAC3G6.12|SNF2 family helicase
Ino80|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1604
Score = 27.1 bits (57), Expect = 2.1
Identities = 14/57 (24%), Positives = 26/57 (45%), Gaps = 6/57 (10%)
Frame = -2
Query: 277 WESIRWQILNGDEIEVSPEHRSLAWRELIINVANN------TPLDNTFRTMFQKADF 125
++S++WQ + DE + S W+ L+ N TP+ NT + ++ F
Sbjct: 966 FQSVKWQYMILDEAQAIKSSSSSRWKSLLAFKCRNRLLLTGTPIQNTMQELWALLHF 1022
>SPCC1020.01c |pma2|SPCC1393.01|P-type proton ATPase Pma2
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1010
Score = 26.6 bits (56), Expect = 2.8
Identities = 12/47 (25%), Positives = 24/47 (51%)
Frame = -2
Query: 388 SNLLGAISHSHAFYKRLTATFSRKKSFGRHIAVVVNMWESIRWQILN 248
S ++ A+ S + R+ A + + H+ + + +W IR Q+LN
Sbjct: 758 SAIIDALKTSRQIFHRMYAYVVYRIALSLHLEIFLGLWLIIRNQLLN 804
>SPBC577.15c |||NASP family histone binding
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 396
Score = 26.6 bits (56), Expect = 2.8
Identities = 11/32 (34%), Positives = 17/32 (53%)
Frame = -2
Query: 214 SLAWRELIINVANNTPLDNTFRTMFQKADFEN 119
+L W+E + NV+NNT L + +F N
Sbjct: 225 ALEWKEKVYNVSNNTLLSEAHYKLALALEFTN 256
>SPAC26A3.12c |dhp1||5'-3' exoribonuclease Dhp1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 991
Score = 26.2 bits (55), Expect = 3.7
Identities = 12/50 (24%), Positives = 27/50 (54%), Gaps = 1/50 (2%)
Frame = -2
Query: 199 ELIIN-VANNTPLDNTFRTMFQKADFENFDYNTPIVYNLKTKTLTMYNER 53
+L+ N N PL+++ + D ++ + +TP+V+ + TK + +R
Sbjct: 520 QLVSNETLQNVPLEDSIASSSAYEDTDSIESSTPVVHPIDTKVSNVGQKR 569
>SPAP27G11.05c |vps41||vacuolar protein sorting-associated protein
Vps41|Schizosaccharomyces pombe|chr 1|||Manual
Length = 886
Score = 25.8 bits (54), Expect = 4.9
Identities = 18/66 (27%), Positives = 29/66 (43%)
Frame = -2
Query: 274 ESIRWQILNGDEIEVSPEHRSLAWRELIINVANNTPLDNTFRTMFQKADFENFDYNTPIV 95
ESI I ++ + E R I + N LDN F + + N+ P++
Sbjct: 771 ESISMAIKYREQEQSGLEFLVKGKRSKNIFLTNLVKLDNPFNSFGDEQLDYNYRKRIPMI 830
Query: 94 YNLKTK 77
Y+L+TK
Sbjct: 831 YDLRTK 836
>SPCC1494.10 ||SPCC70.01|transcription factor |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 964
Score = 25.4 bits (53), Expect = 6.5
Identities = 14/39 (35%), Positives = 19/39 (48%)
Frame = +2
Query: 275 PHIDNNSNMATKRFFSGESSGEPLIKRMAMANSPKKIRE 391
P NS++A+ S SG+ L M+ A SP K E
Sbjct: 85 PFPPKNSSLASPSKISESISGDRLYNHMSSAPSPNKKEE 123
>SPAC1071.10c |pma1||P-type proton ATPase Pma1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 919
Score = 25.4 bits (53), Expect = 6.5
Identities = 11/47 (23%), Positives = 24/47 (51%)
Frame = -2
Query: 388 SNLLGAISHSHAFYKRLTATFSRKKSFGRHIAVVVNMWESIRWQILN 248
S ++ A+ S + R+ + + + H+ + + +W IR Q+LN
Sbjct: 670 SAIIDALKTSRQIFHRMYSYVVYRIALSLHLEIFLGLWLIIRNQLLN 716
>SPAPB1E7.07 |glt1||glutamate synthase Glt1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 2111
Score = 25.0 bits (52), Expect = 8.6
Identities = 16/66 (24%), Positives = 29/66 (43%)
Frame = -2
Query: 229 SPEHRSLAWRELIINVANNTPLDNTFRTMFQKADFENFDYNTPIVYNLKTKTLTMYNERI 50
+P R+ W+EL + + + T R M F DY PI + T ++ ++
Sbjct: 1629 NPLKRTNDWKELSVRLREDELRVQTARCMDCGTPFCQSDYGCPISNKIFTWNDLVFKQQW 1688
Query: 49 RAALNR 32
+ AL +
Sbjct: 1689 KEALTQ 1694
>SPAC22F3.10c |gcs1|apd1|glutamate-cysteine ligase Gcs1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 669
Score = 25.0 bits (52), Expect = 8.6
Identities = 14/36 (38%), Positives = 23/36 (63%), Gaps = 1/36 (2%)
Frame = -2
Query: 433 RHFAH*FTINSFVIFS-NLLGAISHSHAFYKRLTAT 329
+H AH F+ + VIFS ++L S S+A ++ L +T
Sbjct: 426 KHMAHIFSRDPLVIFSDSILQDNSVSNAHFENLNST 461
>SPBC16H5.13 |||WD repeat protein |Schizosaccharomyces pombe|chr
2|||Manual
Length = 1026
Score = 25.0 bits (52), Expect = 8.6
Identities = 13/40 (32%), Positives = 21/40 (52%)
Frame = -2
Query: 487 LVVGHYSKSVIVFIIDTQRHFAH*FTINSFVIFSNLLGAI 368
L+VG S V+VF + T HF I +F+ + + +I
Sbjct: 31 LIVGQCSGQVMVFDVSTDNHFT---LIQTFIAHKHSVSSI 67
>SPAC589.12 ||SPAC688.01|glycosylceramide biosynthesis protein
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 971
Score = 25.0 bits (52), Expect = 8.6
Identities = 15/39 (38%), Positives = 20/39 (51%), Gaps = 1/39 (2%)
Frame = +1
Query: 76 FLFLNCTQ-SVYCSRNFQNRLFETLF*TCCQAVCCWPHL 189
FLFLN + Y + +RLF T F CC+ + W L
Sbjct: 345 FLFLNVIGIAAYKLEDPVHRLFVTAFSVCCECL-AWTSL 382
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,467,160
Number of Sequences: 5004
Number of extensions: 53836
Number of successful extensions: 185
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 175
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 185
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 267622334
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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