Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmnc25e21
(644 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 87 3e-18
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 58 1e-09
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 57 2e-09
SPBC119.13c |prp31||U4/U6 x U5 tri-snRNP complex subunit Prp31|S... 33 0.047
SPCC1840.09 |||NAD dependent epimerase/dehydratase family protei... 26 4.0
SPBP19A11.06 |lid2|SPBP4H10.01|Lid2 complex subunit Lid2 |Schizo... 26 5.3
SPCC1742.01 ||SPCC1795.13, SPCPB16A4.07c|sequence orphan|Schizos... 25 7.1
SPAC1687.05 |pli1||SUMO E3 ligase Pli1|Schizosaccharomyces pombe... 25 9.3
SPAC1002.10c |sgt1||SGT1 family transcriptional regulator Sgt1|S... 25 9.3
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 86.6 bits (205), Expect = 3e-18
Identities = 54/144 (37%), Positives = 79/144 (54%), Gaps = 6/144 (4%)
Frame = +1
Query: 94 RYFKEQDIDEFRECFYLFARS--GQITSLDELTVIMRSLGMSPT---IQELAGYLKGKG- 255
R ++ I EFRE F LF R G ITS +EL V+MRSLG SPT +Q++ + G
Sbjct: 4 RNLTDEQIAEFREAFSLFDRDQDGNITS-NELGVVMRSLGQSPTAAELQDMINEVDADGN 62
Query: 256 GKMSFADFLEVMHIHSRAENLPSEVVNAFKAGDPNKTGVISAKQLRTLLQKWGEGLSARE 435
G + F +FL +M + + EV AFK D + G I+ ++L +L GE LS E
Sbjct: 63 GTIDFTEFLTMMARKMKDTDNEEEVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQEE 122
Query: 436 VDNIFREANVSNSATVRYEDFVKI 507
V ++ REA+ + YE+F ++
Sbjct: 123 VADMIREADTDGDGVINYEEFSRV 146
>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 143
Score = 58.0 bits (134), Expect = 1e-09
Identities = 41/136 (30%), Positives = 66/136 (48%), Gaps = 2/136 (1%)
Frame = +1
Query: 103 KEQDIDEFRECFYLFA--RSGQITSLDELTVIMRSLGMSPTIQELAGYLKGKGGKMSFAD 276
KEQ DE +E F L+ + G I + + ++RSLG++ T ELA G +
Sbjct: 5 KEQT-DEMKEAFVLYDIDKDGLIPT-SHVGSVLRSLGINVTDAELAKLSNELGDAIDEKK 62
Query: 277 FLEVMHIHSRAENLPSEVVNAFKAGDPNKTGVISAKQLRTLLQKWGEGLSAREVDNIFRE 456
F+ + R E + AF+ D + +G I + ++ GE LS EV + +E
Sbjct: 63 FMSFVSNKLRETESEEEYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQLMVQE 122
Query: 457 ANVSNSATVRYEDFVK 504
A+ +NS + Y DFV+
Sbjct: 123 ADPTNSGSFDYYDFVQ 138
>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 141
Score = 57.2 bits (132), Expect = 2e-09
Identities = 42/141 (29%), Positives = 72/141 (51%), Gaps = 6/141 (4%)
Frame = +1
Query: 112 DIDEFRECFYLFAR--SGQI--TSLDELTVIMRSLGMSPTIQELAGYLKGKGGKMSFADF 279
D +++ F LF R +G+I TS+ +L +R+ G +PT+ E+ ++ F
Sbjct: 4 DDSPYKQAFSLFDRHGTGRIPKTSIGDL---LRACGQNPTLAEITEIESTLPAEVDMEQF 60
Query: 280 LEVMHIHSRAE--NLPSEVVNAFKAGDPNKTGVISAKQLRTLLQKWGEGLSAREVDNIFR 453
L+V++ + + P E V F+ D + TG+I +LR +L GE LS E+D + +
Sbjct: 61 LQVLNRPNGFDMPGDPEEFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELLK 120
Query: 454 EANVSNSATVRYEDFVKIACA 516
V + V Y DFV++ A
Sbjct: 121 GVPVKD-GMVNYHDFVQMILA 140
>SPBC119.13c |prp31||U4/U6 x U5 tri-snRNP complex subunit
Prp31|Schizosaccharomyces pombe|chr 2|||Manual
Length = 518
Score = 32.7 bits (71), Expect = 0.047
Identities = 15/54 (27%), Positives = 29/54 (53%)
Frame = +1
Query: 244 KGKGGKMSFADFLEVMHIHSRAENLPSEVVNAFKAGDPNKTGVISAKQLRTLLQ 405
+G G + L++ I A+ LPSEV N F + N ++++ +LR +++
Sbjct: 41 EGNGISAELENDLDITKISDSAQKLPSEVANKFNDNNENIYQLLNSTRLRDIIE 94
>SPCC1840.09 |||NAD dependent epimerase/dehydratase family
protein|Schizosaccharomyces pombe|chr 3|||Manual
Length = 276
Score = 26.2 bits (55), Expect = 4.0
Identities = 12/28 (42%), Positives = 17/28 (60%)
Frame = +1
Query: 28 NRLSKNLFVSNQNTLAPPIKMARYFKEQ 111
N LS N+F + QN LAP + A+ K +
Sbjct: 100 NSLSSNMFKTGQNPLAPKPEEAKQSKNK 127
>SPBP19A11.06 |lid2|SPBP4H10.01|Lid2 complex subunit Lid2
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1513
Score = 25.8 bits (54), Expect = 5.3
Identities = 20/70 (28%), Positives = 31/70 (44%), Gaps = 5/70 (7%)
Frame = -1
Query: 443 LSTSLAESPSPHFCRSVRSCLADITPVLFGSPA---LNAFTTSDGRF--SARECMCITSR 279
L + L+ SP P + ++S L D + +P L F + + S EC+ + S
Sbjct: 715 LRSVLSTSPKPQL-KVLKSLLVDAEKAMLTTPETVNLRDFVQNANSWIDSVNECLKVASL 773
Query: 278 KSANDIFPPL 249
K D PPL
Sbjct: 774 KRKKDKKPPL 783
>SPCC1742.01 ||SPCC1795.13, SPCPB16A4.07c|sequence
orphan|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1563
Score = 25.4 bits (53), Expect = 7.1
Identities = 19/77 (24%), Positives = 32/77 (41%)
Frame = -1
Query: 482 TVALLETFASRKMLSTSLAESPSPHFCRSVRSCLADITPVLFGSPALNAFTTSDGRFSAR 303
++ + TF S K S ++ SPS S + + ++ + S A TTS A
Sbjct: 115 SMTAISTFTSGKEASYAIQASPSTFLPDSTTTSGSQVSNAVEASSTFVADTTSTSCNPAT 174
Query: 302 ECMCITSRKSANDIFPP 252
+ TS ++ PP
Sbjct: 175 VLIVTTSGSTSTSCPPP 191
>SPAC1687.05 |pli1||SUMO E3 ligase Pli1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 727
Score = 25.0 bits (52), Expect = 9.3
Identities = 16/51 (31%), Positives = 30/51 (58%), Gaps = 3/51 (5%)
Frame = -3
Query: 408 FLQKRPQLLSRYHSGLV--RISGLKRVYYL*RKIFGTRM-YVHNFKEIRER 265
FLQ+ P +L R +GL+ ++ + RV+ L ++ GT+ + K++ ER
Sbjct: 6 FLQELPNVLKRLETGLIIPQLKDILRVFGL--RLSGTKAELITRIKQLIER 54
>SPAC1002.10c |sgt1||SGT1 family transcriptional regulator
Sgt1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 590
Score = 25.0 bits (52), Expect = 9.3
Identities = 10/25 (40%), Positives = 16/25 (64%)
Frame = +1
Query: 283 EVMHIHSRAENLPSEVVNAFKAGDP 357
+++H+ + +NL S VNAF DP
Sbjct: 216 KIVHVLQKNKNLISSAVNAFYYRDP 240
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,519,694
Number of Sequences: 5004
Number of extensions: 50676
Number of successful extensions: 118
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 115
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 116
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 289756512
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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