Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmnc25e17
(539 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC1B3.14 |vma3||V-type ATPase subunit c|Schizosaccharomyces po... 185 3e-48
SPAC732.01 |vma11||V-type ATPase proteolipid subunit|Schizosacch... 159 2e-40
SPAC2C4.13 |vma16||V-type ATPase subunit c''|Schizosaccharomyces... 71 1e-13
SPAC212.04c |||S. pombe specific DUF999 family protein 1|Schizos... 32 0.047
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 28 0.77
SPAC227.06 |||Rab GTPase binding |Schizosaccharomyces pombe|chr ... 27 1.3
SPBC19G7.05c |bgs1|cps1, drc1|1,3-beta-glucan synthase catalytic... 27 1.8
SPBC17G9.05 |rct1|cyp6|RRM-containing cyclophilin regulating tra... 27 2.3
SPBC25D12.03c |mcm7||MCM complex subunit Mcm7|Schizosaccharomyce... 26 3.1
SPBC691.04 |||mitochondrial ATP-dependent RNA helicase Mss116 |S... 25 5.4
SPAC27F1.08 |pdt1||Nramp family manganese ion transporter|Schizo... 25 5.4
SPBC15C4.06c ||SPBC21H7.01c|ubiquitin-protein ligase E3 |Schizos... 25 9.5
>SPAC1B3.14 |vma3||V-type ATPase subunit c|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 161
Score = 185 bits (451), Expect = 3e-48
Identities = 91/129 (70%), Positives = 109/129 (84%)
Frame = +2
Query: 152 PIYGPFFGVMGAASAIIFSALGAAYGTAKSGTGIAAMSVMRPELIMKSIIPVVMAGIIAI 331
P+Y PFFGVMG +AI+F++ GAAYGTAK+G GI+AM V+RP+LI+K+ IPVVMAGIIAI
Sbjct: 7 PVYAPFFGVMGCTAAIVFASFGAAYGTAKAGVGISAMGVLRPDLIVKNTIPVVMAGIIAI 66
Query: 332 YGLVVAVLIAGALQEPANYPLYKGFIHLGAGLAVXFSGLAAGFAIXIVGDAGVRGTAXQP 511
YGLVV+VLI+G L++ + LY GFI LGAGL+V +GLAAGFAI IVGDAGVRGTA QP
Sbjct: 67 YGLVVSVLISGNLKQILS--LYSGFIQLGAGLSVGLAGLAAGFAIGIVGDAGVRGTAQQP 124
Query: 512 XLFVGMILI 538
LFV MILI
Sbjct: 125 RLFVAMILI 133
>SPAC732.01 |vma11||V-type ATPase proteolipid
subunit|Schizosaccharomyces pombe|chr 1|||Manual
Length = 162
Score = 159 bits (386), Expect = 2e-40
Identities = 73/129 (56%), Positives = 96/129 (74%)
Frame = +2
Query: 152 PIYGPFFGVMGAASAIIFSALGAAYGTAKSGTGIAAMSVMRPELIMKSIIPVVMAGIIAI 331
PIY FFG G ++++FS LGA YGTA +G GIAA+ RPE++MKS+IPVVM+GII +
Sbjct: 7 PIYSSFFGFAGVCASMVFSCLGAGYGTALAGRGIAAVGAFRPEIVMKSLIPVVMSGIIGV 66
Query: 332 YGLVVAVLIAGALQEPANYPLYKGFIHLGAGLAVXFSGLAAGFAIXIVGDAGVRGTAXQP 511
YGLV++VLIAG + +Y L+ GFIHL AGLAV +G+AAG+AI +VGD GV+ Q
Sbjct: 67 YGLVMSVLIAGDMSPDNDYSLFSGFIHLSAGLAVGLTGVAAGYAIGVVGDRGVQSFMRQD 126
Query: 512 XLFVGMILI 538
+FV M+LI
Sbjct: 127 RIFVSMVLI 135
>SPAC2C4.13 |vma16||V-type ATPase subunit c''|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 199
Score = 70.5 bits (165), Expect = 1e-13
Identities = 36/129 (27%), Positives = 65/129 (50%), Gaps = 6/129 (4%)
Frame = +2
Query: 170 FGVMGAASAIIFSALGAAYGTAKSGTGIAAMSVMRPELIMKSIIPVVMAGIIAIYGLVVA 349
+G++G AS + F +GAA+G GT I +V P + K++I ++ ++AIY L++A
Sbjct: 45 WGLLGIASCVAFGIIGAAWGIFICGTSILGGAVKAPRIKTKNLISIIFCEVVAIYSLIIA 104
Query: 350 VLIAGALQE--PANY----PLYKGFIHLGAGLAVXFSGLAAGFAIXIVGDAGVRGTAXQP 511
++ + + + PA + Y GF G+ V L G + I G + A
Sbjct: 105 IVFSAKINDINPAGFYTKSHYYTGFALFWGGITVGLCNLICGVCVGITGSSAALADAQDA 164
Query: 512 XLFVGMILI 538
LFV ++++
Sbjct: 165 SLFVKVLVV 173
>SPAC212.04c |||S. pombe specific DUF999 family protein
1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 288
Score = 32.3 bits (70), Expect = 0.047
Identities = 21/68 (30%), Positives = 30/68 (44%)
Frame = +2
Query: 296 IIPVVMAGIIAIYGLVVAVLIAGALQEPANYPLYKGFIHLGAGLAVXFSGLAAGFAIXIV 475
II +AG+IA + +++ IAG + G ++ G L LA GF I
Sbjct: 193 IITATIAGVIAAFSVIITATIAGVIAAMV------GILYFGHWLVYKILILAFGFKIVTS 246
Query: 476 GDAGVRGT 499
GD V T
Sbjct: 247 GDVCVSNT 254
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 28.3 bits (60), Expect = 0.77
Identities = 13/34 (38%), Positives = 17/34 (50%)
Frame = -3
Query: 501 AVPRTPASPTMXMAKPAARPENXTAKPAPKWMNP 400
+VP P++P M P+A P A AP NP
Sbjct: 1713 SVPPPPSAPPMPAGPPSAPPPPLPASSAPSVPNP 1746
>SPAC227.06 |||Rab GTPase binding |Schizosaccharomyces pombe|chr
1|||Manual
Length = 249
Score = 27.5 bits (58), Expect = 1.3
Identities = 22/72 (30%), Positives = 32/72 (44%)
Frame = +2
Query: 155 IYGPFFGVMGAASAIIFSALGAAYGTAKSGTGIAAMSVMRPELIMKSIIPVVMAGIIAIY 334
+YGPF+ A+ FS Y +G G + S+ K +I A II Y
Sbjct: 87 LYGPFWITTTVIQALFFSNSITEYARYATGHGTSGYSI-------KKLISA--ASIIYGY 137
Query: 335 GLVVAVLIAGAL 370
++AVL+ G L
Sbjct: 138 TTIIAVLLWGIL 149
>SPBC19G7.05c |bgs1|cps1, drc1|1,3-beta-glucan synthase catalytic
subunit Bgs1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1729
Score = 27.1 bits (57), Expect = 1.8
Identities = 21/95 (22%), Positives = 47/95 (49%), Gaps = 6/95 (6%)
Frame = +2
Query: 131 NKMAENNP---IYGPFFG-VMGAASAIIFSALGAAYGTAKSGTGIAAMSVMR-PELIMKS 295
+K+A ++P I F+G ++G + F+ + + ++ G S LI+ S
Sbjct: 1424 DKIAMDSPRARITTMFYGEILGPLGTLFFTCIPFLFINSQPGNDDETQSTNAFIRLIIMS 1483
Query: 296 IIPVVMAGIIAIYGLVVAVLIAGALQEPA-NYPLY 397
+ P+V++ IIA + + +++ L + + Y +Y
Sbjct: 1484 VAPLVLSAIIAFFFFCLGIMLRPILGDRSKTYGVY 1518
>SPBC17G9.05 |rct1|cyp6|RRM-containing cyclophilin regulating
transcription Rct1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 432
Score = 26.6 bits (56), Expect = 2.3
Identities = 10/23 (43%), Positives = 14/23 (60%)
Frame = -1
Query: 110 TGPKNCDDYLHTLTPRNYFCTDK 42
TG KNCDD+L +P + D+
Sbjct: 402 TGNKNCDDHLRDKSPERRYRYDR 424
>SPBC25D12.03c |mcm7||MCM complex subunit Mcm7|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 760
Score = 26.2 bits (55), Expect = 3.1
Identities = 14/38 (36%), Positives = 22/38 (57%)
Frame = -2
Query: 409 DEPFVKGVVGWLLEGTSNQDSHDQTVDGNNTRHDDRND 296
DEPF+K +L+ SN++S+ VD N+ D +D
Sbjct: 48 DEPFLKSKYMDILQKISNRESNVINVDLNDLYEFDPSD 85
>SPBC691.04 |||mitochondrial ATP-dependent RNA helicase Mss116
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 535
Score = 25.4 bits (53), Expect = 5.4
Identities = 8/13 (61%), Positives = 10/13 (76%)
Frame = -1
Query: 110 TGPKNCDDYLHTL 72
TGP N DDY+H +
Sbjct: 369 TGPSNTDDYIHRI 381
>SPAC27F1.08 |pdt1||Nramp family manganese ion
transporter|Schizosaccharomyces pombe|chr 1|||Manual
Length = 521
Score = 25.4 bits (53), Expect = 5.4
Identities = 12/35 (34%), Positives = 20/35 (57%), Gaps = 4/35 (11%)
Frame = +2
Query: 386 YPLYKGFIHLGAG----LAVXFSGLAAGFAIXIVG 478
Y L K ++ + G +A+ FSG++AG+ I G
Sbjct: 359 YDLLKEYVSISCGRLFAVALLFSGMSAGYVCTIAG 393
>SPBC15C4.06c ||SPBC21H7.01c|ubiquitin-protein ligase E3
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 556
Score = 24.6 bits (51), Expect = 9.5
Identities = 13/54 (24%), Positives = 26/54 (48%)
Frame = +2
Query: 209 ALGAAYGTAKSGTGIAAMSVMRPELIMKSIIPVVMAGIIAIYGLVVAVLIAGAL 370
AL +G + + + P+L+ I V+ G+I + GL + + +GA+
Sbjct: 354 ALVEYFGNIDATARLGVIISKEPKLLGLYIFIGVLLGLIGVIGLFICLHFSGAM 407
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,365,138
Number of Sequences: 5004
Number of extensions: 48943
Number of successful extensions: 167
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 163
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 165
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 221892220
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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