Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmnc25b24
(622 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC16G5.14c |rps3||40S ribosomal protein S3|Schizosaccharomyces... 298 5e-82
SPAPB1E7.07 |glt1||glutamate synthase Glt1 |Schizosaccharomyces ... 28 1.3
SPBC146.13c |myo1||myosin type I|Schizosaccharomyces pombe|chr 2... 27 2.9
SPAC3H5.09c |||conserved fungal protein|Schizosaccharomyces pomb... 26 5.1
SPBC4F6.06 |kin1||microtubule affinity-regulating kinase Kin1 |S... 25 8.8
SPBPB2B2.12c |||UDP-glucose 4-epimerase|Schizosaccharomyces pomb... 25 8.8
SPBC646.03 |||glutamyl-tRNA amidotransferase|Schizosaccharomyces... 25 8.8
SPBC691.03c |apl3||AP-2 adaptor complex subunit Alp3 |Schizosacc... 25 8.8
SPBC25H2.15 |||programmed cell death protein homolog|Schizosacch... 25 8.8
>SPBC16G5.14c |rps3||40S ribosomal protein S3|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 249
Score = 298 bits (731), Expect = 5e-82
Identities = 143/187 (76%), Positives = 160/187 (85%)
Frame = +3
Query: 36 AVNNISKKRKFVGDGVFKAELNEFLTRELAEDGYSGVEVRVTPIRSEIIIMATRTQSVLG 215
A ISKKRKFV DGVF AELNEF TREL+E+GYSG EVRVTP RSEIII AT TQ VLG
Sbjct: 3 AAFTISKKRKFVADGVFYAELNEFFTRELSEEGYSGCEVRVTPSRSEIIIRATHTQDVLG 62
Query: 216 EKGRRIRELTSVVQKRFNIPEQSVELYAEKVATRGLCAIAQAESLRYKLIGGLAVRRACY 395
EKGRRIRELT++VQKRF E +VELYAEKV RGLCA+AQ ESLRYKL+ GLAVRRA Y
Sbjct: 63 EKGRRIRELTALVQKRFKFAENTVELYAEKVQNRGLCAVAQCESLRYKLLAGLAVRRAAY 122
Query: 396 GVLRFIMESGARGCEVVVSGKLRGQRAKSMKFVDGLMIHSGDPCNDYVNTATRHVLLRQG 575
GVLR++ME+GA+GCEVV+SGKLR RAKSMKF DG MIHSG P D++++ATRHVLLRQG
Sbjct: 123 GVLRYVMEAGAKGCEVVISGKLRAARAKSMKFADGFMIHSGQPAVDFIDSATRHVLLRQG 182
Query: 576 VLGIKVK 596
VLG+KVK
Sbjct: 183 VLGVKVK 189
>SPAPB1E7.07 |glt1||glutamate synthase Glt1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 2111
Score = 27.9 bits (59), Expect = 1.3
Identities = 15/52 (28%), Positives = 26/52 (50%)
Frame = +3
Query: 402 LRFIMESGARGCEVVVSGKLRGQRAKSMKFVDGLMIHSGDPCNDYVNTATRH 557
+ F ME + + ++ +L+ S K D ++I GD ND + T+ RH
Sbjct: 1868 IHFAMEFLHKNTKSLLDSELKDGNYISAKGKDVIVIGGGDTGNDCLGTSVRH 1919
>SPBC146.13c |myo1||myosin type I|Schizosaccharomyces pombe|chr
2|||Manual
Length = 1217
Score = 26.6 bits (56), Expect = 2.9
Identities = 19/62 (30%), Positives = 31/62 (50%), Gaps = 1/62 (1%)
Frame = +3
Query: 186 MATRTQSVLGEKGRRIRELTSVVQKRFNIPEQSVELYAEKVATRGLCAI-AQAESLRYKL 362
MATR Q RR E + +QK +N + ++EL E+V G + + + RY +
Sbjct: 726 MATRIQRAWRSYVRRRSEAAACIQKLWNRNKVNMEL--ERVRNEGTKLLQGKKQRRRYSI 783
Query: 363 IG 368
+G
Sbjct: 784 LG 785
>SPAC3H5.09c |||conserved fungal protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 2685
Score = 25.8 bits (54), Expect = 5.1
Identities = 9/31 (29%), Positives = 16/31 (51%), Gaps = 1/31 (3%)
Frame = +2
Query: 467 STCQINEVCR-WTHDPLWRPLQ*LRQHCYQT 556
+ C + CR W P W+ + + Q+C+ T
Sbjct: 1420 TVCNRKKACRLWNFKPHWQVITRIPQYCHDT 1450
>SPBC4F6.06 |kin1||microtubule affinity-regulating kinase Kin1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 891
Score = 25.0 bits (52), Expect = 8.8
Identities = 9/24 (37%), Positives = 15/24 (62%)
Frame = -1
Query: 304 FSAYNSTDCSGMLNRFCTTEVSSR 233
+ +Y S+DC G+L+R T+ R
Sbjct: 360 YPSYLSSDCKGLLSRMLVTDPLKR 383
>SPBPB2B2.12c |||UDP-glucose 4-epimerase|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 713
Score = 25.0 bits (52), Expect = 8.8
Identities = 10/26 (38%), Positives = 14/26 (53%)
Frame = +3
Query: 465 GQRAKSMKFVDGLMIHSGDPCNDYVN 542
G+R K + F D H G P DY++
Sbjct: 218 GRREKLLVFGDDYDSHDGTPIRDYIH 243
>SPBC646.03 |||glutamyl-tRNA amidotransferase|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 471
Score = 25.0 bits (52), Expect = 8.8
Identities = 22/70 (31%), Positives = 28/70 (40%), Gaps = 2/70 (2%)
Frame = -3
Query: 500 SIYKLH*FGTLTTQLARYNNFTTTGTRFHDETENTIASTTYSETSDKLVS*RFGLG--DS 327
S+Y + T+ LA+YN E I+ST S V R LG
Sbjct: 296 SVYSTMAYAEATSNLAKYNTIAFGNCLDEKFEEEIISSTARSFFLGDEVKKRLLLGAYSL 355
Query: 326 AKTTSSHLFS 297
A+ SS LFS
Sbjct: 356 ARMNSSDLFS 365
>SPBC691.03c |apl3||AP-2 adaptor complex subunit Alp3
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 878
Score = 25.0 bits (52), Expect = 8.8
Identities = 9/18 (50%), Positives = 12/18 (66%)
Frame = -3
Query: 290 FYRLLWNVESLLYYGSQL 237
FYRL W + +LY SQ+
Sbjct: 643 FYRLCWKDKGILYQDSQI 660
>SPBC25H2.15 |||programmed cell death protein
homolog|Schizosaccharomyces pombe|chr 2|||Manual
Length = 396
Score = 25.0 bits (52), Expect = 8.8
Identities = 15/53 (28%), Positives = 23/53 (43%)
Frame = -1
Query: 364 ISLYLRDSAWAIAQRPRVATFSAYNSTDCSGMLNRFCTTEVSSRILRPFSPST 206
+ L L+ A+ ++ S+ +N F T SSR L PFS +T
Sbjct: 101 VRLPLKSDIEAVKSPKAISHLEEKKSSPKEKKVNPFAITSESSRGLNPFSDAT 153
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,732,640
Number of Sequences: 5004
Number of extensions: 57172
Number of successful extensions: 144
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 136
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 144
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 273658928
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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