Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= bmnc24p02
         (734 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces...   304   7e-84
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar...   164   1e-41
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch...   159   4e-40
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|...   158   7e-40
SPBC19G7.16 |iws1||transcription elongation factor complex subun...    27   2.8  
SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizos...    27   3.7  
SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual        26   4.8  
SPBC15D4.03 |slm9||hira protein Slm9|Schizosaccharomyces pombe|c...    26   4.8  
SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1 |Schizos...    26   6.4  
SPBC646.12c |gap1|src1, sar1|GTPase activating protein Gap1|Schi...    25   8.5  
SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1 |Schiz...    25   8.5  

>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 448

 Score =  304 bits (747), Expect = 7e-84
 Identities = 137/194 (70%), Positives = 157/194 (80%)
 Frame = +2

Query: 116 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYV 295
           MREIVHIQAGQCGNQ+GA FW  I+DEHG+D  G YHG S+ Q ER+NVY+NEA+GGKYV
Sbjct: 1   MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNEAAGGKYV 60

Query: 296 PRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVV 475
           PRA+LVDLEPGTMD+V+SG FG +FRPDN ++GQSGAGN WAKGHYTEGAEL D+VLDVV
Sbjct: 61  PRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVV 120

Query: 476 RKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTYSVVPSPKVSDTVV 655
           R+EAE+CD LQGFQ                   KIREEYPDR+M T+SV P+PK SDTVV
Sbjct: 121 RREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDTVV 180

Query: 656 EPYNATLSVHQLVE 697
           EPYNATLS+HQLVE
Sbjct: 181 EPYNATLSMHQLVE 194



 Score = 28.3 bits (60), Expect = 1.2
 Identities = 10/12 (83%), Positives = 12/12 (100%)
 Frame = +1

Query: 697 NTDETYCIDNEA 732
           N+DET+CIDNEA
Sbjct: 195 NSDETFCIDNEA 206


>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
           2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 449

 Score =  164 bits (399), Expect = 1e-41
 Identities = 79/196 (40%), Positives = 113/196 (57%), Gaps = 2/196 (1%)
 Frame = +2

Query: 116 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLER--INVYYNEASGGK 289
           MREI+ I  GQ G QIG   WE+   EHGI P G  + ++  Q      + +++E   GK
Sbjct: 1   MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60

Query: 290 YVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLD 469
           YVPR+I VDLEP  +D VR+GP+  +F P+  + G+  A NN+A+GHYT G ELVD V D
Sbjct: 61  YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120

Query: 470 VVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTYSVVPSPKVSDT 649
            +R+ A++C  LQGF                    ++  EY  +    +SV P+P+VS +
Sbjct: 121 KIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTS 180

Query: 650 VVEPYNATLSVHQLVE 697
           VVEPYN+ L+ H  ++
Sbjct: 181 VVEPYNSVLTTHATLD 196


>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 455

 Score =  159 bits (386), Expect = 4e-40
 Identities = 76/200 (38%), Positives = 114/200 (57%), Gaps = 6/200 (3%)
 Frame = +2

Query: 116 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTG------AYHGDSDLQLERINVYYNEA 277
           MRE++ +  GQ G QIG   WE+   EHGI P G        H ++    +    +++E 
Sbjct: 1   MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENSEVHKNNSYLNDGFGTFFSET 60

Query: 278 SGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVD 457
             GK+VPR+I VDLEP  +D VR+GP+  +F P+  V G+  A NN+A+GHYT G E++D
Sbjct: 61  GQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMID 120

Query: 458 SVLDVVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTYSVVPSPK 637
           SVL+ +R+ A++C  LQGF                    ++  EY  +    +SV P+P+
Sbjct: 121 SVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAPQ 180

Query: 638 VSDTVVEPYNATLSVHQLVE 697
           VS +VVEPYN+ L+ H  ++
Sbjct: 181 VSTSVVEPYNSVLTTHATLD 200


>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 446

 Score =  158 bits (384), Expect = 7e-40
 Identities = 72/194 (37%), Positives = 120/194 (61%), Gaps = 3/194 (1%)
 Frame = +2

Query: 119 REIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYVP 298
           REI+ +QAGQCGNQIG++FW+ +  EHGI P G     +   ++R +V++ ++   +Y+P
Sbjct: 3   REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFATEGVDRKDVFFYQSDDTRYIP 62

Query: 299 RAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQS--GAGNNWAKGHYTEGAELVDSVLDV 472
           RAIL+DLEP  ++++ S  +G ++ P+N +  ++  GAGNNWA G Y+    + + ++D+
Sbjct: 63  RAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDM 121

Query: 473 VRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTYSVVP-SPKVSDT 649
           + +EA+  D L+GF                    ++ + YP +I+ TYSV P S  VSD 
Sbjct: 122 IDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVSDV 181

Query: 650 VVEPYNATLSVHQL 691
           VV+PYN+ L++ +L
Sbjct: 182 VVQPYNSLLALKRL 195


>SPBC19G7.16 |iws1||transcription elongation factor complex subunit
           Iws1 |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 428

 Score = 27.1 bits (57), Expect = 2.8
 Identities = 10/34 (29%), Positives = 22/34 (64%)
 Frame = +2

Query: 155 NQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERI 256
           N++G    E+++++  +DPT A   + DLQ++ +
Sbjct: 133 NELGENEEEVLTEQKQLDPTLAAKKELDLQMDAV 166


>SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein
           Pof11|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 506

 Score = 26.6 bits (56), Expect = 3.7
 Identities = 14/35 (40%), Positives = 19/35 (54%), Gaps = 1/35 (2%)
 Frame = +2

Query: 350 GPFGQIFRPDNFVFGQSGAG-NNWAKGHYTEGAEL 451
           GP+G +F P  F+F  +G    NW+   Y E A L
Sbjct: 157 GPYGTMFLPQQFIFDSNGRPLLNWSY-LYKEHAHL 190


>SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 234

 Score = 26.2 bits (55), Expect = 4.8
 Identities = 12/30 (40%), Positives = 19/30 (63%)
 Frame = +2

Query: 419 AKGHYTEGAELVDSVLDVVRKEAESCDCLQ 508
           A+GH   G ELV +  D +RK++E+   L+
Sbjct: 183 AEGHPDVGVELVRAGADTLRKDSENHTALE 212


>SPBC15D4.03 |slm9||hira protein Slm9|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 807

 Score = 26.2 bits (55), Expect = 4.8
 Identities = 16/46 (34%), Positives = 23/46 (50%)
 Frame = -3

Query: 492 DSASFRTTSKTESTSSAPSV*CPLAQLLPAPDCPKTKLSGRKICPK 355
           ++A+ RTTS T+    +PS       L P P    +  S R+ CPK
Sbjct: 390 NAAADRTTSPTQGQPESPS---KSILLRPPPSIASSPESKRRKCPK 432


>SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1
            |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1063

 Score = 25.8 bits (54), Expect = 6.4
 Identities = 12/44 (27%), Positives = 20/44 (45%)
 Frame = +2

Query: 371  RPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDC 502
            RP   +F     G++   G   +  E  D ++ +  +  ESCDC
Sbjct: 955  RPSRLIF-YDNCGDSSGAGLCNKAYEHTDELITMAIERIESCDC 997


>SPBC646.12c |gap1|src1, sar1|GTPase activating protein
           Gap1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 766

 Score = 25.4 bits (53), Expect = 8.5
 Identities = 15/46 (32%), Positives = 27/46 (58%)
 Frame = -2

Query: 268 VVYIDALQLQVRVPMVSTGGVDAVLVGDDLPELSSDLVAALTSLDM 131
           ++Y+DA  L +++  +   G  A  V  DLP L +D V++L S+ +
Sbjct: 511 ILYVDAKTLFIQLLRLLPSGHPATRVPLDLP-LIADSVSSLKSMSL 555


>SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 255

 Score = 25.4 bits (53), Expect = 8.5
 Identities = 13/32 (40%), Positives = 16/32 (50%)
 Frame = -3

Query: 408 PAPDCPKTKLSGRKICPKGPERTESMVPGSKS 313
           P+   PK  L  R I P GPE  +  + GS S
Sbjct: 20  PSTPPPKEVLHTRVIVPNGPEEIKLRLVGSHS 51


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,820,394
Number of Sequences: 5004
Number of extensions: 54346
Number of successful extensions: 160
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 145
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 152
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 347244562
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)