Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmnc23n14
(756 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC29A4.15 |||serine-tRNA ligase|Schizosaccharomyces pombe|chr ... 119 4e-28
SPAC9G1.13c |||histone acetyltransferase complex subunit Swc4 |S... 30 0.31
SPBC18E5.11c |edc3|SPBC23G7.01c|enhancer of mRNA decapping Edc3 ... 25 1.4
SPBC19C7.02 |ubr1|SPBC32F12.14|N-end-recognizing protein Ubr1|Sc... 27 2.9
SPAC821.13c ||SPAC955.01c|P-type ATPase |Schizosaccharomyces pom... 27 2.9
SPBC1604.18c |||vacuolar sorting protein |Schizosaccharomyces po... 26 5.0
SPBC1773.10c |||asparagine-tRNA ligase Ded81 |Schizosaccharomyce... 26 5.0
SPCC1906.02c |||CUE domain protein Cue3 |Schizosaccharomyces pom... 26 6.7
SPAC19B12.03 |bgs3||1,3-beta-glucan synthase subunit Bgs3|Schizo... 26 6.7
SPAC12B10.14c |ppk2||serine/threonine protein kinase Ppk2 |Schiz... 26 6.7
SPCC1739.11c |cdc11||SIN component scaffold protein Cdc11|Schizo... 25 8.8
SPAC167.03c |snu66||U4/U6 x U5 tri-snRNP complex subunit Snu66 |... 25 8.8
>SPAC29A4.15 |||serine-tRNA ligase|Schizosaccharomyces pombe|chr
1|||Manual
Length = 450
Score = 119 bits (287), Expect = 4e-28
Identities = 85/219 (38%), Positives = 117/219 (53%), Gaps = 4/219 (1%)
Frame = +2
Query: 104 VLDLDLFRADKDGNPDKIRENQKKRFKDVALVDAVVEQDTLWRKLRHEADNFNKLKNVCS 283
+LD++LF+ +K GNP+ IRE+Q+KR DV +VD V+E W LR E DN NK N
Sbjct: 1 MLDINLFQVEKGGNPEIIRESQRKRGADVGVVDKVIEMYKEWVSLRFELDNTNKSINRVQ 60
Query: 284 KEIGLKMKNKEPSGSEDEPVPSEIANNLVNLTGDNLKPLTVNQIKKVRVLIDNAISKNDE 463
KEIGLKMK KE + SE+ +LT ++ + LI+ +KN E
Sbjct: 61 KEIGLKMKAKEDA--------SELLEEKNSLT------------ERKKNLIEQETAKNKE 100
Query: 464 GLLAAEKARSAALREVGNHLHESVPVDDDEDHNLVERTDGDCG--FRKK--YSHVDLICM 631
L + +GN +H+SVPV DED+N + R G KK SH +++
Sbjct: 101 ML--------NVVSSIGNIVHDSVPVSMDEDNNEIIRKWAPEGVTVEKKNCLSHHEVLTR 152
Query: 632 IDGMDGERGSAVAGGRGYYLKGPAVFLEQALVQLSLRML 748
+DG D ERG V+G RGY+L+ VF AL+Q L L
Sbjct: 153 LDGYDPERGVKVSGHRGYFLRQYGVFFNLALIQYGLDFL 191
>SPAC9G1.13c |||histone acetyltransferase complex subunit Swc4
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 411
Score = 30.3 bits (65), Expect = 0.31
Identities = 18/44 (40%), Positives = 24/44 (54%)
Frame = +2
Query: 287 EIGLKMKNKEPSGSEDEPVPSEIANNLVNLTGDNLKPLTVNQIK 418
EIG K K+K P+ E P I+ L +L G+N PL + Q K
Sbjct: 16 EIGNKQKSKTPT----ERRPEGISRELYSLLGENSAPLAIYQKK 55
>SPBC18E5.11c |edc3|SPBC23G7.01c|enhancer of mRNA decapping Edc3
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 454
Score = 25.0 bits (52), Expect(2) = 1.4
Identities = 17/53 (32%), Positives = 27/53 (50%)
Frame = +2
Query: 332 DEPVPSEIANNLVNLTGDNLKPLTVNQIKKVRVLIDNAISKNDEGLLAAEKAR 490
DE +P+E+ TGD LKP+T Q+ + L AI+K ++ A+
Sbjct: 199 DENIPAELHTT----TGDILKPITPEQLSQGIAL---AIAKTSTDIVVENAAQ 244
Score = 21.4 bits (43), Expect(2) = 1.4
Identities = 8/27 (29%), Positives = 15/27 (55%)
Frame = +2
Query: 599 KKYSHVDLICMIDGMDGERGSAVAGGR 679
+ ++ L+C++ G +AVA GR
Sbjct: 263 RNHNSQPLVCILVGSHDHASAAVAAGR 289
>SPBC19C7.02 |ubr1|SPBC32F12.14|N-end-recognizing protein
Ubr1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1958
Score = 27.1 bits (57), Expect = 2.9
Identities = 16/43 (37%), Positives = 23/43 (53%), Gaps = 4/43 (9%)
Frame = +2
Query: 488 RSAALR----EVGNHLHESVPVDDDEDHNLVERTDGDCGFRKK 604
RS+ LR + N++ E + + D DH + ER G G RKK
Sbjct: 1203 RSSVLRLFPADDANYVSEVLDIPDSLDHEIQERPFGLAGKRKK 1245
>SPAC821.13c ||SPAC955.01c|P-type ATPase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1562
Score = 27.1 bits (57), Expect = 2.9
Identities = 14/38 (36%), Positives = 22/38 (57%)
Frame = +2
Query: 587 CGFRKKYSHVDLICMIDGMDGERGSAVAGGRGYYLKGP 700
CG K+YS V ++ +DG++G V+GG+ L P
Sbjct: 1116 CGVIKEYSTVVVMGSLDGVEGS-DETVSGGQRLSLDRP 1152
>SPBC1604.18c |||vacuolar sorting protein |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 449
Score = 26.2 bits (55), Expect = 5.0
Identities = 13/33 (39%), Positives = 16/33 (48%)
Frame = +1
Query: 640 NGWREGISCSRWSWLLSKRSCSIPGTSPRSTFS 738
N WRE I S WS L+ R + + S FS
Sbjct: 47 NTWREAIETSAWSGKLNSRIILVFDETFESAFS 79
>SPBC1773.10c |||asparagine-tRNA ligase Ded81 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 568
Score = 26.2 bits (55), Expect = 5.0
Identities = 14/44 (31%), Positives = 24/44 (54%), Gaps = 2/44 (4%)
Frame = +2
Query: 383 DNLKPLTVNQIKKVRVLIDNAISKNDEG--LLAAEKARSAALRE 508
+ +P+T N +KK + ++ A K + AA AR+AA +E
Sbjct: 62 EEFEPITTNALKKAKKGVEQAAKKKAKAAEAEAAAAARAAAAKE 105
>SPCC1906.02c |||CUE domain protein Cue3 |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 581
Score = 25.8 bits (54), Expect = 6.7
Identities = 21/75 (28%), Positives = 39/75 (52%), Gaps = 6/75 (8%)
Frame = +2
Query: 194 LVDAVVEQDTLWRKLRHEADNFNKLKNVCSKEI------GLKMKNKEPSGSEDEPVPSEI 355
L+D++ ++ ++ EA F +LK++ + EI GL+ K+ SG V S+
Sbjct: 206 LLDSLDLRNCIYVVFEDEA-LFRRLKDIGNPEIRMMLDSGLRDWRKQRSGRSSSHVASKG 264
Query: 356 ANNLVNLTGDNLKPL 400
N +VN+ ++K L
Sbjct: 265 INKVVNINPGDVKSL 279
>SPAC19B12.03 |bgs3||1,3-beta-glucan synthase subunit
Bgs3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1826
Score = 25.8 bits (54), Expect = 6.7
Identities = 11/47 (23%), Positives = 22/47 (46%)
Frame = -1
Query: 366 RLLAISEGTGSSSEPLGSLFFIFKPISLLHTFFSLLKLSASWRNFLH 226
+ L +S+G+ + S +F+ I + + S +W +FLH
Sbjct: 376 KALLLSDGSRIMDADVASRYFLLADIQWQRVCYKSFRESRTWLHFLH 422
>SPAC12B10.14c |ppk2||serine/threonine protein kinase Ppk2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 665
Score = 25.8 bits (54), Expect = 6.7
Identities = 12/17 (70%), Positives = 12/17 (70%)
Frame = -1
Query: 159 RILSGLPSLSARNKSKS 109
R L GLPSLS RN KS
Sbjct: 130 RSLHGLPSLSRRNSKKS 146
>SPCC1739.11c |cdc11||SIN component scaffold protein
Cdc11|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1045
Score = 25.4 bits (53), Expect = 8.8
Identities = 17/62 (27%), Positives = 26/62 (41%)
Frame = +3
Query: 171 RRDLKMLPLWMQSSSKTPCGGNCATKLTISIN*RMYVAKKLV*K*RTRNPVALKTNPSPR 350
R D +P W Q ++K P + +L + + K T+NP +N S R
Sbjct: 59 RNDAPDVPQWKQVNAKNPVARDIFARLDLENMFEESSKQSPPSKSPTKNPSKKSSNNSSR 118
Query: 351 RS 356
RS
Sbjct: 119 RS 120
>SPAC167.03c |snu66||U4/U6 x U5 tri-snRNP complex subunit Snu66
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 649
Score = 25.4 bits (53), Expect = 8.8
Identities = 25/111 (22%), Positives = 50/111 (45%), Gaps = 4/111 (3%)
Frame = +2
Query: 149 DKIRENQKKRFKDVALVDAVVEQDTL----WRKLRHEADNFNKLKNVCSKEIGLKMKNKE 316
D E +K D ++ + V+Q+ W+K E N K + + SK L+ KN+
Sbjct: 29 DISEEKPQKELSDASVKSSYVDQEQQAYENWKKQEQEEINRKKEEELKSKFEKLRQKNER 88
Query: 317 PSGSEDEPVPSEIANNLVNLTGDNLKPLTVNQIKKVRVLIDNAISKNDEGL 469
++ + + +A + ++ T I K+R +++++KN GL
Sbjct: 89 RRRTQGKTLAETLAEEDDQIDAND----TRAWILKMR---NSSLNKNGNGL 132
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,960,719
Number of Sequences: 5004
Number of extensions: 59411
Number of successful extensions: 199
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 188
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 198
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 361294920
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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