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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= bmnc22p14
         (736 letters)

Database: nematostella 
           59,808 sequences; 16,821,457 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SB_31686| Best HMM Match : No HMM Matches (HMM E-Value=.)              40   3e-05
SB_24958| Best HMM Match : S-methyl_trans (HMM E-Value=1.6e-40)        45   6e-05
SB_1494| Best HMM Match : No HMM Matches (HMM E-Value=.)               42   7e-04
SB_53787| Best HMM Match : S-methyl_trans (HMM E-Value=0.00013)        32   0.55 
SB_10202| Best HMM Match : No HMM Matches (HMM E-Value=.)              30   1.7  
SB_976| Best HMM Match : No HMM Matches (HMM E-Value=.)                29   3.9  
SB_53869| Best HMM Match : ABC_membrane (HMM E-Value=0.34)             29   5.2  
SB_54782| Best HMM Match : No HMM Matches (HMM E-Value=.)              28   6.8  
SB_50885| Best HMM Match : UCH (HMM E-Value=4.9e-06)                   28   9.0  

>SB_31686| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 149

 Score = 40.3 bits (90), Expect(2) = 3e-05
 Identities = 18/30 (60%), Positives = 19/30 (63%)
 Frame = +3

Query: 477 KPLIAGSVGPYGAYLHDTSEYTGNYADNTT 566
           KP +AGSV PYG    D SEY GNY D  T
Sbjct: 24  KPQVAGSVCPYGTCQSDGSEYHGNYVDTMT 53



 Score = 25.0 bits (52), Expect(2) = 3e-05
 Identities = 12/23 (52%), Positives = 14/23 (60%)
 Frame = +3

Query: 291 FLRAGSDIIETNTYQASVDGFVK 359
           FL  GSDII T TYQ   +  +K
Sbjct: 2   FLTHGSDIITTATYQVCSNDRIK 24


>SB_24958| Best HMM Match : S-methyl_trans (HMM E-Value=1.6e-40)
          Length = 560

 Score = 45.2 bits (102), Expect = 6e-05
 Identities = 46/155 (29%), Positives = 73/155 (47%)
 Frame = +3

Query: 231 LWSARFLKTHPQDVINTHLDFLRAGSDIIETNTYQASVDGFVKHLNLTVEESYELIKSAV 410
           +W+   +  HP+ V   H +FLRAG+D+I+  T+ A  D   K L   V   Y      +
Sbjct: 129 VWTPECVVEHPEAVRQLHREFLRAGADVIQAFTF-AMQD---KPL---VSAGYSYKWDEI 181

Query: 411 EFARTARDLYLQECQESNLSGRKPLIAGSVGPYGAYLHDTSEYTGNYADNTTKETIKNWH 590
             A +         +E +  G   L +GS+   G      S +    A   +KE IK   
Sbjct: 182 SHAGSIL------AKEVSDEGEFALSSGSLCETG------SLFIKGLA---SKEEIKQRF 226

Query: 591 RTRIQALVEAGVDILAFETIPCQKEAEALVEILKE 695
           R +++  V+ G+D+L  E I   +EAE +VE++KE
Sbjct: 227 RDQVKIFVDTGMDLLIAEYISHVQEAEWMVEVMKE 261


>SB_1494| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 429

 Score = 41.5 bits (93), Expect = 7e-04
 Identities = 50/175 (28%), Positives = 75/175 (42%)
 Frame = +3

Query: 171 DGGFSTQLTCHAGHTADGDPLWSARFLKTHPQDVINTHLDFLRAGSDIIETNTYQASVDG 350
           DGGF   L    G+   G   W+      +P+ V   H +FLRAGSD+++T T+ AS D 
Sbjct: 51  DGGFVFALE-KRGYVKAGP--WTPEATVENPEAVRQLHREFLRAGSDVMQTFTFYASDD- 106

Query: 351 FVKHLNLTVEESYELIKSAVEFARTARDLYLQECQESNLSGRKPLIAGSVGPYGAYLHDT 530
             K  N   E S +   +A+  A  A DL  +   E +      L+AG V          
Sbjct: 107 --KLQNRGNEASKKFGCAAINEA--ACDLAREVADEGD-----ALVAGGVC--------- 148

Query: 531 SEYTGNYADNTTKETIKNWHRTRIQALVEAGVDILAFETIPCQKEAEALVEILKE 695
              T  Y     KE  +   + ++   ++  VD +  E     +EA   VE+LK+
Sbjct: 149 --QTPTYLSGKGKEACQAEFQKQVDVFIKKDVDFIIAEYYEHVEEAVWAVELLKK 201


>SB_53787| Best HMM Match : S-methyl_trans (HMM E-Value=0.00013)
          Length = 167

 Score = 31.9 bits (69), Expect = 0.55
 Identities = 17/66 (25%), Positives = 34/66 (51%)
 Frame = +3

Query: 453 QESNLSGRKPLIAGSVGPYGAYLHDTSEYTGNYADNTTKETIKNWHRTRIQALVEAGVDI 632
           +E++  G +  +AG++GP    L  +         N T + + + +  + + L++ GVD+
Sbjct: 50  EEADFRGVEKFVAGAMGPTNRTLSISPTVECPGFRNVTFDELVDAYTEQARGLLDGGVDV 109

Query: 633 LAFETI 650
           L  ETI
Sbjct: 110 LLVETI 115


>SB_10202| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 1165

 Score = 30.3 bits (65), Expect = 1.7
 Identities = 18/49 (36%), Positives = 26/49 (53%), Gaps = 2/49 (4%)
 Frame = -1

Query: 184 ENPPSNTKTVFEDTSKFDIIS*KNKFIVRKLLQHTY--RIFYNNLIRCN 44
           E+P  +   +F D+ + DI    NKF   K ++ T   RI YNN  +CN
Sbjct: 408 ESPDEDIPQMFVDSLESDIKDIYNKFKFSKKVKMTLRDRIDYNNATQCN 456


>SB_976| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 447

 Score = 29.1 bits (62), Expect = 3.9
 Identities = 15/42 (35%), Positives = 27/42 (64%), Gaps = 2/42 (4%)
 Frame = +3

Query: 591 RTRIQALVEAGVDILAFETIPCQKEAE-ALVEILKE-YPNMK 710
           + R+ ALVEAGVD++  ++       + +L+  +KE YPN++
Sbjct: 242 KARLHALVEAGVDVVVIDSSQGNSIYQLSLISHIKENYPNLQ 283


>SB_53869| Best HMM Match : ABC_membrane (HMM E-Value=0.34)
          Length = 403

 Score = 28.7 bits (61), Expect = 5.2
 Identities = 28/128 (21%), Positives = 55/128 (42%)
 Frame = +3

Query: 237 SARFLKTHPQDVINTHLDFLRAGSDIIETNTYQASVDGFVKHLNLTVEESYELIKSAVEF 416
           S+RF  T+    I+    F+++    ++      S     KH+N   + +Y  I++    
Sbjct: 55  SSRFTSTNIISEIDVEGHFIKSTQAALDLGGIIDSSLSMTKHVNSICKSAYHSIRNIGRI 114

Query: 417 ARTARDLYLQECQESNLSGRKPLIAGSVGPYGAYLHDTSEYTGNYADNTTKETIKNWHRT 596
            +     YL   + ++L+ R+P+ A  +  +     D  EY     +N T++    WHR 
Sbjct: 115 RK-----YLD--RNNSLNKRRPVEASDLPCF-----DNHEYD---CENLTEKLDSEWHRE 159

Query: 597 RIQALVEA 620
           +  A+  A
Sbjct: 160 KANAVRNA 167


>SB_54782| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 435

 Score = 28.3 bits (60), Expect = 6.8
 Identities = 15/41 (36%), Positives = 22/41 (53%)
 Frame = -2

Query: 531 MCHVNMHHKDLPNQL*VVSDLKGLILGILASISRVQFLQIQ 409
           M HV +HHK LP    +  D+  ++ G +A +  V F  IQ
Sbjct: 378 MFHVKLHHKTLPWYY-IALDVAIIVFGAVACLCGVVFSGIQ 417


>SB_50885| Best HMM Match : UCH (HMM E-Value=4.9e-06)
          Length = 286

 Score = 27.9 bits (59), Expect = 9.0
 Identities = 16/45 (35%), Positives = 23/45 (51%)
 Frame = +3

Query: 135 NFEVSSKTVFVLDGGFSTQLTCHAGHTADGDPLWSARFLKTHPQD 269
           N E  S+TV+ L     T +T H G   DG  +   RF+ +HP +
Sbjct: 194 NSESRSRTVYQL-----TAVTSHMGDVDDGHYVTYRRFVPSHPDE 233


  Database: nematostella
    Posted date:  Oct 22, 2007  1:22 PM
  Number of letters in database: 16,821,457
  Number of sequences in database:  59,808
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 22,382,690
Number of Sequences: 59808
Number of extensions: 483366
Number of successful extensions: 1292
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 1168
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1290
length of database: 16,821,457
effective HSP length: 80
effective length of database: 12,036,817
effective search space used: 1974037988
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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