Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= bmnc22n10
         (754 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC1773.15 |||membrane transporter|Schizosaccharomyces pombe|ch...    33   0.033
SPAC12G12.04 |hsp60|hsp60|mitochondrial heat shock protein Hsp60...    28   1.6  
SPBC16A3.04 |rsm25||mitochondrial ribosomal protein subunit Rsm2...    27   2.2  
SPCC576.15c |ksg1||serine/threonine protein kinase Ksg1|Schizosa...    27   3.8  
SPAC227.07c |pab1||protein phosphatase regulatory subunit Pab1|S...    26   5.0  
SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy...    26   5.0  
SPBC31E1.01c |atg2|mug36, SPBC660.18c|autophagy associated prote...    26   6.6  
SPAC1250.01 |snf21|SPAC29A4.21|ATP-dependent DNA helicase Snf21|...    26   6.6  
SPAC17G8.14c |pck1|SPAC22H10.01c|protein kinase C |Schizosacchar...    26   6.6  
SPBC887.10 |mcs4||two-component response regulator |Schizosaccha...    26   6.6  
SPAC1F3.06c |spo15||sporulation protein Spo15|Schizosaccharomyce...    26   6.6  
SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces ...    25   8.8  
SPBC1709.08 |cft1||cleavage factor one Cft1 |Schizosaccharomyces...    25   8.8  
SPAC30D11.04c |nup124||nucleoporin Nup124|Schizosaccharomyces po...    25   8.8  
SPAC14C4.05c |mug61||Sad1 interacting factor|Schizosaccharomyces...    25   8.8  
SPBC3D6.10 |apn2||AP-endonuclease Apn2|Schizosaccharomyces pombe...    25   8.8  

>SPBC1773.15 |||membrane transporter|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 497

 Score = 33.5 bits (73), Expect = 0.033
 Identities = 18/62 (29%), Positives = 33/62 (53%)
 Frame = -3

Query: 458 IEKSILQSMNLKSGSWILSGLCMGVKCRAASA*TPYWSRVCFGLESHLFLVGHQRAWSDQ 279
           I+KS+ + + LK+  W++  LC+    +     T  +S +  G+ + L +VG+Q  W   
Sbjct: 36  IDKSLNRKLKLKTDLWVMPLLCLISAFQYMDKSTSNYSSI-MGIRTDLNMVGNQYNWVGT 94

Query: 278 SF 273
           SF
Sbjct: 95  SF 96


>SPAC12G12.04 |hsp60|hsp60|mitochondrial heat shock protein
           Hsp60|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 582

 Score = 27.9 bits (59), Expect = 1.6
 Identities = 11/30 (36%), Positives = 18/30 (60%)
 Frame = +1

Query: 178 DLNEKRTMVVKGDMHIGGVMLKLTESFGKE 267
           ++++  T+   GD HIG ++ K  E  GKE
Sbjct: 174 EISQVATISANGDTHIGELLAKAMERVGKE 203


>SPBC16A3.04 |rsm25||mitochondrial ribosomal protein subunit
           Rsm25|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 220

 Score = 27.5 bits (58), Expect = 2.2
 Identities = 10/22 (45%), Positives = 13/22 (59%)
 Frame = +1

Query: 244 LTESFGKEFKKDWSDHALWWPT 309
           L  SF  E KK W+  +LW+ T
Sbjct: 9   LLRSFAAEMKKPWTAESLWYET 30


>SPCC576.15c |ksg1||serine/threonine protein kinase
           Ksg1|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 592

 Score = 26.6 bits (56), Expect = 3.8
 Identities = 10/27 (37%), Positives = 18/27 (66%)
 Frame = +1

Query: 130 GEIVGDGSWNLTIYVTDLNEKRTMVVK 210
           GEI+G+GS++  +  T+ + KR   +K
Sbjct: 102 GEILGEGSYSTVLTATENSTKREYAIK 128


>SPAC227.07c |pab1||protein phosphatase regulatory subunit
           Pab1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 463

 Score = 26.2 bits (55), Expect = 5.0
 Identities = 20/51 (39%), Positives = 26/51 (50%), Gaps = 1/51 (1%)
 Frame = +1

Query: 322 LSRPKHTLDQYGVHADAA-LHFTPMHKPLRIQLPDLRFIDCKIDFSIDTFS 471
           LSR   TL  + V+ + A +   P+H  LR +L DL   DC  D    TFS
Sbjct: 300 LSRDYLTLKIWDVNMEKAPVKTIPLHDVLRSKLCDLYENDCIFDKFECTFS 350


>SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 433

 Score = 26.2 bits (55), Expect = 5.0
 Identities = 10/38 (26%), Positives = 22/38 (57%)
 Frame = -3

Query: 503 IPRFLHSCITALNVSIEKSILQSMNLKSGSWILSGLCM 390
           IP+   +CI+  +V I  S+L ++ +  G+ ++ G  +
Sbjct: 327 IPQLFQNCISECDVDIRASLLNNVIVCGGTSLMQGFSL 364


>SPBC31E1.01c |atg2|mug36, SPBC660.18c|autophagy associated protein
            Mug36|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1646

 Score = 25.8 bits (54), Expect = 6.6
 Identities = 10/34 (29%), Positives = 19/34 (55%)
 Frame = +1

Query: 433  IDCKIDFSIDTFSAVIQLCKNLGIRHSEELSLCY 534
            +D ++    D+   +I++  +LG    EE+S CY
Sbjct: 984  LDAQVQSCADSTELLIKVLSDLGSTEDEEISDCY 1017


>SPAC1250.01 |snf21|SPAC29A4.21|ATP-dependent DNA helicase
           Snf21|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1199

 Score = 25.8 bits (54), Expect = 6.6
 Identities = 12/26 (46%), Positives = 16/26 (61%)
 Frame = +1

Query: 547 SHLKQNYQNLKEAKRIKNTQAPDTNT 624
           S +K  Y  + E  R+KNTQ+  TNT
Sbjct: 534 SRIKWIYMIIDEGHRMKNTQSKLTNT 559


>SPAC17G8.14c |pck1|SPAC22H10.01c|protein kinase C
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 988

 Score = 25.8 bits (54), Expect = 6.6
 Identities = 14/35 (40%), Positives = 21/35 (60%)
 Frame = +1

Query: 628 IAATRGSSNSLDRLNGPCPATPPPPRSLSATPVAS 732
           +AAT+  ++ L+    P P TP   RSL+ TP +S
Sbjct: 589 LAATQPVTSVLN--TSPLPKTPEKDRSLNVTPSSS 621


>SPBC887.10 |mcs4||two-component response regulator
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 522

 Score = 25.8 bits (54), Expect = 6.6
 Identities = 13/43 (30%), Positives = 25/43 (58%)
 Frame = +1

Query: 619 NTFIAATRGSSNSLDRLNGPCPATPPPPRSLSATPVASQQNGT 747
           N+   +TR   ++ ++L     ATP   RS+S + + ++Q+GT
Sbjct: 237 NSIGMSTRSDESTAEKLAKAEVATPTNSRSISHSSLYTKQSGT 279


>SPAC1F3.06c |spo15||sporulation protein Spo15|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1957

 Score = 25.8 bits (54), Expect = 6.6
 Identities = 17/65 (26%), Positives = 30/65 (46%)
 Frame = +1

Query: 457 IDTFSAVIQLCKNLGIRHSEELSLCYPLEASHLKQNYQNLKEAKRIKNTQAPDTNTFIAA 636
           +D+    IQ  K   +R SEE      LEA +L++   NLK        Q  D ++ ++ 
Sbjct: 684 VDSLEKNIQTLKE-DLRKSEEALRFSKLEAKNLREVIDNLKGKHETLEAQRNDLHSSLSD 742

Query: 637 TRGSS 651
            + ++
Sbjct: 743 AKNTN 747


>SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 644

 Score = 25.4 bits (53), Expect = 8.8
 Identities = 12/48 (25%), Positives = 24/48 (50%), Gaps = 2/48 (4%)
 Frame = +1

Query: 139 VGDGSWNLTIYVTDLNEKRTMVVKGDMHIGGVML--KLTESFGKEFKK 276
           +G G++++++   +          GD H+GG     +L   F +EFK+
Sbjct: 198 LGGGTFDVSLLTIEEGIFEVKATAGDTHLGGEDFDSRLVNHFAQEFKR 245


>SPBC1709.08 |cft1||cleavage factor one Cft1 |Schizosaccharomyces
            pombe|chr 2|||Manual
          Length = 1441

 Score = 25.4 bits (53), Expect = 8.8
 Identities = 14/43 (32%), Positives = 21/43 (48%), Gaps = 5/43 (11%)
 Frame = +1

Query: 136  IVGDGSWNLTIYVTDLNEKRT-----MVVKGDMHIGGVMLKLT 249
            +V D S NL +   D     +     +V +GD HIG V+  +T
Sbjct: 1270 VVADTSGNLRLLAYDPENPESHSGERLVTRGDFHIGNVITAMT 1312


>SPAC30D11.04c |nup124||nucleoporin Nup124|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1159

 Score = 25.4 bits (53), Expect = 8.8
 Identities = 16/63 (25%), Positives = 30/63 (47%)
 Frame = -1

Query: 199 SSVFRSDPSHKS*GSTTHHQLFHRPPTFSLVRLIKLINYKCHYRARIASLNFFEITSTTA 20
           +S+  S    K  GST+ HQ+     + SL+ +++ ++ +      I+S+    IT    
Sbjct: 140 NSLHPSSALSKKLGSTSQHQIATPKSSASLLNILRSLHDEQKNTLNISSVKQDRITEANP 199

Query: 19  TIE 11
           T E
Sbjct: 200 TCE 202


>SPAC14C4.05c |mug61||Sad1 interacting factor|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 844

 Score = 25.4 bits (53), Expect = 8.8
 Identities = 14/65 (21%), Positives = 24/65 (36%)
 Frame = +1

Query: 529 CYPLEASHLKQNYQNLKEAKRIKNTQAPDTNTFIAATRGSSNSLDRLNGPCPATPPPPRS 708
           C P     +++N+Q   ++    N      N  +  + G+S +    N     T P   S
Sbjct: 287 CNPSNLLSVRKNFQQSSDSYLKSNKSFDQLNNLVGLSTGNSENFTPENNSFSWTHPKKNS 346

Query: 709 LSATP 723
            S  P
Sbjct: 347 SSPLP 351


>SPBC3D6.10 |apn2||AP-endonuclease Apn2|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 523

 Score = 25.4 bits (53), Expect = 8.8
 Identities = 21/92 (22%), Positives = 40/92 (43%), Gaps = 1/92 (1%)
 Frame = +1

Query: 430 FIDCKIDFSIDTFSAVIQLCKNLGIRHSEELSLCYPLEASH-LKQNYQNLKEAKRIKNTQ 606
           ++D K ++     S  +   K   +  +   S   P +  H + Q++ ++K+ K    TQ
Sbjct: 299 YLDLKEEYEGKKLSNFLSHSKEPPLLSTAHHSAYRPSKNIHSMFQHFNSMKKNKNNSPTQ 358

Query: 607 APDTNTFIAATRGSSNSLDRLNGPCPATPPPP 702
           +   N   +A+ GSS ++ R N        PP
Sbjct: 359 SE--NVSASASSGSSPTVSRANSVIDVDAYPP 388


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,848,942
Number of Sequences: 5004
Number of extensions: 56590
Number of successful extensions: 196
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 183
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 196
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 359287726
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)