Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmnc21h14
(534 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces po... 112 4e-26
SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces po... 79 3e-16
SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces ... 59 4e-10
SPAC959.05c |||protein disulfide isomerase |Schizosaccharomyces ... 44 1e-05
SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces ... 44 2e-05
SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyc... 39 4e-04
SPBC12D12.07c |trx2||mitochondrial thioredoxin Trx2|Schizosaccha... 39 5e-04
SPBC577.08c |txl1|trx3|thioredoxin-like I protein Txl1|Schizosac... 36 0.003
SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|c... 27 1.3
SPCC4B3.10c |ipk1||inositol 1,3,4,5,6-pentakisphosphate |Schizos... 27 2.3
SPAC29E6.08 |tbp1|tdf1, tbp, SPAC30.12|TATA-binding protein |Sch... 25 5.4
SPAC4F8.13c |rng2||IQGAP|Schizosaccharomyces pombe|chr 1|||Manual 25 5.4
SPACUNK4.07c |cta4|sev4, SPAPYUK71.01|P-type ATPase, calcium tra... 25 5.4
SPAC19E9.01c |nup40||nucleoporin Nup40|Schizosaccharomyces pombe... 25 7.1
SPBP16F5.05c |||ribosome biogenesis protein Nop8|Schizosaccharom... 25 7.1
SPBC557.03c |pim1|dcd1, ptr2|GDP/GTP exchange factor |Schizosacc... 25 7.1
SPAC664.07c |rad9||checkpoint clamp complex protein Rad9|Schizos... 25 9.4
>SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 492
Score = 112 bits (269), Expect = 4e-26
Identities = 52/123 (42%), Positives = 78/123 (63%)
Frame = +2
Query: 158 YLCKAAEEDVLDLTDSDFSAVLSQHDTALVMFYAPWCGHCKRLKPEYAVAAGLLKTDDPP 337
+ C +AE V + + +++ +V FYAPWCGHCK L PEY AA L+ D
Sbjct: 17 FFCASAE--VPKVNKEGLNELITADKVLMVKFYAPWCGHCKALAPEYESAADELEKDG-- 72
Query: 338 VALAKVDCTEGGKSTCEQFSVSGYPTLKIFRKGELSSEYNGPRESNGIVKYMRAQVGPSS 517
++L +VDCTE G C ++S+ GYPTL +F+ G+ S+Y+GPR+ + +VKYMR Q+ P+
Sbjct: 73 ISLVEVDCTEEG-DLCSEYSIRGYPTLNVFKNGKQISQYSGPRKHDALVKYMRKQLLPTV 131
Query: 518 KEL 526
K +
Sbjct: 132 KPI 134
Score = 69.3 bits (162), Expect = 3e-13
Identities = 43/122 (35%), Positives = 62/122 (50%), Gaps = 5/122 (4%)
Frame = +2
Query: 173 AEEDVLDLTDSDFS-AVLSQHDTALVMFYAPWCGHCKRLKPEYAVAAGLLKTDDPPVALA 349
++ED++ L +F V+ + LV FYAPWCGHCK L P Y A +DD V +A
Sbjct: 353 SQEDLVVLVADNFDDIVMDETKDVLVEFYAPWCGHCKNLAPTYEKLAEEY-SDDSNVVVA 411
Query: 350 KVDCTEGGKSTCEQFSVSGYPTLKIFRKGELSS--EYNGPRESNGIVKYM--RAQVGPSS 517
K+D TE S S+SG+PT+ F+ + + Y G R + ++ A P
Sbjct: 412 KIDATENDISV----SISGFPTIMFFKANDKVNPVRYEGDRTLEDLSAFIDKHASFEPIK 467
Query: 518 KE 523
KE
Sbjct: 468 KE 469
>SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 363
Score = 79.4 bits (187), Expect = 3e-16
Identities = 48/152 (31%), Positives = 78/152 (51%), Gaps = 6/152 (3%)
Frame = +2
Query: 95 KAPAKFEMFGSLKFVLLLGIIYLCKAAEEDVLDLTDSDFSAVLSQHDTALVMFYAPWCGH 274
+ P F +F + F L+ G+ + ++L +F + +LV+FYAPWCG+
Sbjct: 4 RIPTLFTLFLAC-FSLVSGVFGYSPMFGSNTIELNSKNFRKFVKAKGPSLVVFYAPWCGY 62
Query: 275 CKRLKPEYAVAAGLLKTDDPPVALAKVDC-TEGGKSTCEQFSVSGYPTLKIF---RKGE- 439
CK+L P Y A L + P A VDC + ++ C Q+ V G+PT+K+ KG
Sbjct: 63 CKKLVPTYQKLASNLHSLLPVTA---VDCDADQNRAVCSQYQVQGFPTIKLVYPSSKGSS 119
Query: 440 -LSSEYNGPRESNGIVKYMRAQVGPSSKELLT 532
S++YNG R + K++ + PS ++LT
Sbjct: 120 LSSTDYNGDRSYKSLQKFVSDSI-PSKVKILT 150
>SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 359
Score = 59.3 bits (137), Expect = 4e-10
Identities = 33/112 (29%), Positives = 55/112 (49%), Gaps = 3/112 (2%)
Frame = +2
Query: 182 DVLDLTDSDFS-AVLSQHDTALVMFYAPWCGHCKRLKPEYAVAAGLLKTDDPPVALAKVD 358
+V++L +F V+ LV FYA WCG+CKRL P Y + K ++P V + K++
Sbjct: 141 NVVELDSLNFDKVVMDDKKDVLVEFYADWCGYCKRLAPTYETLGKVFK-NEPNVEIVKIN 199
Query: 359 CTEGGKSTCEQFSVSGYPTLKIFRKGELSSE--YNGPRESNGIVKYMRAQVG 508
+ V+ +PT+K F K + Y G R +++Y+ + G
Sbjct: 200 -ADVFADIGRLHEVASFPTIKFFPKDDKDKPELYEGDRSLESLIEYINKKSG 250
Score = 58.8 bits (136), Expect = 5e-10
Identities = 29/98 (29%), Positives = 52/98 (53%), Gaps = 1/98 (1%)
Frame = +2
Query: 239 ALVMFYAPWCGHCKRLKPEYAVAAGLLKTDDPPVALAKVDCTEGGKSTCEQFSVSGYPTL 418
AL+ FYA WCGHCK L P Y G L D V + K+D + +++ ++G+PTL
Sbjct: 42 ALIEFYATWCGHCKSLAPVYE-ELGALFEDHNDVLIGKID-ADTHSDVADKYHITGFPTL 99
Query: 419 KIF-RKGELSSEYNGPRESNGIVKYMRAQVGPSSKELL 529
F G +Y+ R+ + + +++ + G ++++
Sbjct: 100 IWFPPDGSEPVQYSNARDVDSLTQFVSEKTGIKKRKIV 137
>SPAC959.05c |||protein disulfide isomerase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 632
Score = 44.4 bits (100), Expect = 1e-05
Identities = 26/84 (30%), Positives = 44/84 (52%)
Frame = +2
Query: 239 ALVMFYAPWCGHCKRLKPEYAVAAGLLKTDDPPVALAKVDCTEGGKSTCEQFSVSGYPTL 418
A VMF + HC+ AV + + + D + +A+V+C E K C F + +PT
Sbjct: 199 AFVMFVS--LKHCEDCFHWEAVWSSITRNTDERLKMAQVNCDE-EKEMCNHFHIKKFPTF 255
Query: 419 KIFRKGELSSEYNGPRESNGIVKY 490
++F +G S +YNGP + ++ Y
Sbjct: 256 RVF-QGFDSIQYNGPLKYQQLLSY 278
>SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 726
Score = 43.6 bits (98), Expect = 2e-05
Identities = 28/112 (25%), Positives = 54/112 (48%), Gaps = 4/112 (3%)
Frame = +2
Query: 194 LTDSDFSAVLSQHDTALVMFYAPWCGHCKRLKP--EYAVAAGLLKTDDPPVALAKVDCTE 367
LTD+D + +S+ T + +Y P CG CKRL P + V + + +VDC++
Sbjct: 31 LTDNDLESEVSK-GTWFIKYYLPSCGACKRLGPMWDNMVEKAKEQVEGSNFHFGEVDCSK 89
Query: 368 GGKSTCEQFSVSGYPTLKIFRKGELSSE--YNGPRESNGIVKYMRAQVGPSS 517
S+C ++ PTL +++ GE+ E + ++ ++ + P +
Sbjct: 90 -ELSSCA--NIRAVPTLYLYQNGEIVEEVPFGASTSEASLLDFVETHLNPDT 138
Score = 39.9 bits (89), Expect = 2e-04
Identities = 24/97 (24%), Positives = 44/97 (45%)
Frame = +2
Query: 200 DSDFSAVLSQHDTALVMFYAPWCGHCKRLKPEYAVAAGLLKTDDPPVALAKVDCTEGGKS 379
D+D A L+ + + FY+ C C + + A ++ + +A ++C K
Sbjct: 288 DADIDAALTDKEGWFIQFYSSECDDCDDVSTAWYAMANRMRGK---LNVAHINCAVS-KR 343
Query: 380 TCEQFSVSGYPTLKIFRKGELSSEYNGPRESNGIVKY 490
C+Q+S+ +PT +F K E EY G +V +
Sbjct: 344 ACKQYSIQYFPTF-LFFKEEAFVEYVGLPNEGDLVSF 379
>SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 103
Score = 39.1 bits (87), Expect = 4e-04
Identities = 22/86 (25%), Positives = 38/86 (44%)
Frame = +2
Query: 203 SDFSAVLSQHDTALVMFYAPWCGHCKRLKPEYAVAAGLLKTDDPPVALAKVDCTEGGKST 382
S+F +++ Q +V F+A WCG CK + P++ + KVD + +
Sbjct: 9 SEFKSIVCQDKLVVVDFFATWCGPCKAIAPKFEQFSNTYS----DATFIKVDVDQLSEIA 64
Query: 383 CEQFSVSGYPTLKIFRKGELSSEYNG 460
E V P+ +++ GE E G
Sbjct: 65 AEA-GVHAMPSFFLYKNGEKIEEIVG 89
>SPBC12D12.07c |trx2||mitochondrial thioredoxin
Trx2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 121
Score = 38.7 bits (86), Expect = 5e-04
Identities = 23/78 (29%), Positives = 36/78 (46%)
Frame = +2
Query: 206 DFSAVLSQHDTALVMFYAPWCGHCKRLKPEYAVAAGLLKTDDPPVALAKVDCTEGGKSTC 385
D++ +S +V FYA WCG CK LKP L + + V+ +
Sbjct: 27 DYNTRISADKVTVVDFYADWCGPCKYLKP----FLEKLSEQNQKASFIAVN-ADKFSDIA 81
Query: 386 EQFSVSGYPTLKIFRKGE 439
++ V PT+ +FRKG+
Sbjct: 82 QKNGVYALPTMVLFRKGQ 99
>SPBC577.08c |txl1|trx3|thioredoxin-like I protein
Txl1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 290
Score = 36.3 bits (80), Expect = 0.003
Identities = 20/62 (32%), Positives = 26/62 (41%)
Frame = +2
Query: 254 YAPWCGHCKRLKPEYAVAAGLLKTDDPPVALAKVDCTEGGKSTCEQFSVSGYPTLKIFRK 433
YA WCG CK + P ++ A K P AKV+ E + V PT F
Sbjct: 27 YADWCGPCKAISPLFSQLAS--KYASPKFVFAKVNVDE-QRQIASGLGVKAMPTFVFFEN 83
Query: 434 GE 439
G+
Sbjct: 84 GK 85
>SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|chr
2|||Manual
Length = 244
Score = 27.5 bits (58), Expect = 1.3
Identities = 17/73 (23%), Positives = 29/73 (39%)
Frame = +2
Query: 242 LVMFYAPWCGHCKRLKPEYAVAAGLLKTDDPPVALAKVDCTEGGKSTCEQFSVSGYPTLK 421
L+ FYAPW CK++ + A D K++ E E F V+ P
Sbjct: 24 LLNFYAPWAAPCKQMNQVFDQFA----KDTKNAVFLKIE-AEKFSDIAESFDVNAVPLFV 78
Query: 422 IFRKGELSSEYNG 460
+ ++ + +G
Sbjct: 79 LIHGAKVLARISG 91
>SPCC4B3.10c |ipk1||inositol 1,3,4,5,6-pentakisphosphate
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 640
Score = 26.6 bits (56), Expect = 2.3
Identities = 10/23 (43%), Positives = 15/23 (65%)
Frame = +3
Query: 186 FSILQIPTFRLFYLNMIQPWSCF 254
F LQ+ FR + N+++PW CF
Sbjct: 208 FYCLQLQMFRKMH-NIVRPWDCF 229
>SPAC29E6.08 |tbp1|tdf1, tbp, SPAC30.12|TATA-binding protein
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 231
Score = 25.4 bits (53), Expect = 5.4
Identities = 12/44 (27%), Positives = 21/44 (47%)
Frame = -1
Query: 135 NFSDPNISNLAGAFQQNRTNYSAFQPNLTNESIKERTRTGKLKI 4
+F+ P ++ A AF N + PN NE+ E +G ++
Sbjct: 2 DFALPTTASQASAFMNNSSLTFPVLPNANNEATNETADSGDAEV 45
>SPAC4F8.13c |rng2||IQGAP|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1489
Score = 25.4 bits (53), Expect = 5.4
Identities = 10/31 (32%), Positives = 18/31 (58%)
Frame = -1
Query: 507 PTWARMYLTMPLDSLGPLYSEESSPFLKIFS 415
P ++Y+T+ D +G E +SP +K+ S
Sbjct: 1414 PQKEKLYITISADEVGKFILEATSPTVKVSS 1444
>SPACUNK4.07c |cta4|sev4, SPAPYUK71.01|P-type ATPase, calcium
transporting Cta4 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1211
Score = 25.4 bits (53), Expect = 5.4
Identities = 17/50 (34%), Positives = 27/50 (54%)
Frame = +2
Query: 176 EEDVLDLTDSDFSAVLSQHDTALVMFYAPWCGHCKRLKPEYAVAAGLLKT 325
EE V+D+ + D +AVL L + +P+ CK P+ V A +L+T
Sbjct: 324 EEAVIDVDELDKNAVLFGGTRVLQVTQSPF---CKLKTPDNGVPAIVLRT 370
>SPAC19E9.01c |nup40||nucleoporin Nup40|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 371
Score = 25.0 bits (52), Expect = 7.1
Identities = 15/41 (36%), Positives = 24/41 (58%), Gaps = 2/41 (4%)
Frame = -1
Query: 126 DPNISNLAGAFQQNRTNYSAF--QPNLTNESIKERTRTGKL 10
DP+ + ++GA Q + T+ F P LTN+ I E +R G +
Sbjct: 156 DPSFT-MSGAPQDSNTSVIVFGFPPELTNQVIAEFSRFGTI 195
>SPBP16F5.05c |||ribosome biogenesis protein
Nop8|Schizosaccharomyces pombe|chr 2|||Manual
Length = 146
Score = 25.0 bits (52), Expect = 7.1
Identities = 11/28 (39%), Positives = 17/28 (60%)
Frame = +2
Query: 152 IIYLCKAAEEDVLDLTDSDFSAVLSQHD 235
+IY C+AA+E++LD LS+ D
Sbjct: 6 LIYACRAADEELLDEIIEKCPQELSRRD 33
>SPBC557.03c |pim1|dcd1, ptr2|GDP/GTP exchange factor
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 539
Score = 25.0 bits (52), Expect = 7.1
Identities = 11/19 (57%), Positives = 12/19 (63%)
Frame = +2
Query: 320 KTDDPPVALAKVDCTEGGK 376
KTDD PV AK D T G+
Sbjct: 501 KTDDSPVTDAKPDVTSNGE 519
>SPAC664.07c |rad9||checkpoint clamp complex protein
Rad9|Schizosaccharomyces pombe|chr 1|||Manual
Length = 426
Score = 24.6 bits (51), Expect = 9.4
Identities = 11/25 (44%), Positives = 13/25 (52%)
Frame = +2
Query: 371 GKSTCEQFSVSGYPTLKIFRKGELS 445
G S C + V G P L F KG+ S
Sbjct: 265 GSSICAYYGVPGKPILLTFAKGKNS 289
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,261,219
Number of Sequences: 5004
Number of extensions: 45061
Number of successful extensions: 129
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 119
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 124
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 220420454
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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