Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmnc21b21
(685 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC24C9.12c |||glycine hydroxymethyltransferase |Schizosaccharo... 238 5e-64
SPAC18G6.04c |shm2||serine hydroxymethyltransferase Shm2 |Schizo... 235 4e-63
SPAC167.01 |ppk4||serine/threonine protein kinase Ppk4 |Schizosa... 31 0.20
SPAC688.10 |rev3||DNA polymerase zeta catalytic subunit Rev3|Sch... 31 0.20
SPBC32H8.13c |mok12||alpha-1,3-glucan synthase Mok12|Schizosacch... 29 0.83
SPAC4A8.12c |sds22||protein phosphatase regulatory subunit Sds22... 27 1.9
SPBC577.06c |||phosphatidylinositol kinase |Schizosaccharomyces ... 27 1.9
SPAC16A10.01 |||DUF1212 family protein|Schizosaccharomyces pombe... 27 3.3
SPAC1F3.05 |||adaptin |Schizosaccharomyces pombe|chr 1|||Manual 27 3.3
SPAC30.01c |sec72|sec7b|Sec7 domain|Schizosaccharomyces pombe|ch... 26 4.4
SPAC869.08 |pcm2||protein-L-isoaspartate O-methyltransferase |Sc... 26 5.8
SPBC11G11.07 ||SPBC18H10.01|karyopherin|Schizosaccharomyces pomb... 26 5.8
SPAC20H4.10 |ufd2||ubiquitin-protein ligase E4 |Schizosaccharomy... 25 7.7
SPAC3F10.17 |||ribosome biogenesis protein Ltv1|Schizosaccharomy... 25 7.7
>SPAC24C9.12c |||glycine hydroxymethyltransferase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 467
Score = 238 bits (583), Expect = 5e-64
Identities = 109/185 (58%), Positives = 137/185 (74%)
Frame = +1
Query: 130 LWEADPELFDIIVKEKDRQRAGLEMIASENFTSVPVLQCLSSCLHNKYSEGMPNQRYYGG 309
L E DP + +I+ E DRQR+ + +IASENFTS V+ L S + NKYSEG P RYYGG
Sbjct: 12 LKEQDPTVAEIMRHEADRQRSSVVLIASENFTSRAVMDALGSVMSNKYSEGYPGARYYGG 71
Query: 310 NEYIDEIEILAQNRSLEAYRLKSEEWGVNVQPYSGSPANFAVYTGIVEPHGRIMGLDLPD 489
N++ID+IE L Q R+L A+ L +WGVNVQ SGSPAN VY I+ PHGR+MGLDLP
Sbjct: 72 NKFIDQIETLCQERALAAFNLDPAKWGVNVQCLSGSPANMQVYQAIMPPHGRLMGLDLPS 131
Query: 490 GGHLTHGFFTATKKISATSIFFESMPYKVDPKSGLIDYDKLAETAKLFKPRLIIAGMSCY 669
GGHL+HG+ T TKKISA S +FESMPY+VDP +GLIDYD L A+LF+P++++AG S Y
Sbjct: 132 GGHLSHGYQTDTKKISAVSTYFESMPYRVDPNTGLIDYDMLEHDAQLFRPKILVAGTSAY 191
Query: 670 SRCLD 684
R +D
Sbjct: 192 CRLID 196
>SPAC18G6.04c |shm2||serine hydroxymethyltransferase Shm2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 472
Score = 235 bits (575), Expect = 4e-63
Identities = 105/191 (54%), Positives = 138/191 (72%)
Frame = +1
Query: 112 KLLNSNLWEADPELFDIIVKEKDRQRAGLEMIASENFTSVPVLQCLSSCLHNKYSEGMPN 291
KLL + L E DP ++ I+ EK RQ+ + +IASENFTS V+ L S + NKYSEG P
Sbjct: 12 KLLKAPLAECDPTVYKILESEKSRQKESIALIASENFTSRAVMDALGSIMQNKYSEGYPG 71
Query: 292 QRYYGGNEYIDEIEILAQNRSLEAYRLKSEEWGVNVQPYSGSPANFAVYTGIVEPHGRIM 471
RYYGGNE+ID+ E L Q R+LEA+ L E+WGVNVQP+SGSPAN Y +++PH R+M
Sbjct: 72 ARYYGGNEFIDQAERLCQTRALEAFHLDGEKWGVNVQPHSGSPANLQAYQAVMKPHDRLM 131
Query: 472 GLDLPDGGHLTHGFFTATKKISATSIFFESMPYKVDPKSGLIDYDKLAETAKLFKPRLII 651
GLDLP GGHL+HGF T K ISA S +F +MPY V+ ++G+IDYD L + A F+P++I+
Sbjct: 132 GLDLPHGGHLSHGFSTPQKAISAVSTYFSTMPYNVNKETGIIDYDSLEKAAIQFRPKVIV 191
Query: 652 AGMSCYSRCLD 684
AG S Y+R +D
Sbjct: 192 AGASAYARLVD 202
>SPAC167.01 |ppk4||serine/threonine protein kinase Ppk4
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1072
Score = 30.7 bits (66), Expect = 0.20
Identities = 16/62 (25%), Positives = 33/62 (53%)
Frame = +1
Query: 166 VKEKDRQRAGLEMIASENFTSVPVLQCLSSCLHNKYSEGMPNQRYYGGNEYIDEIEILAQ 345
V+E+D L+M+ EN + + + ++CLH+ + + R Y G++ +D + +L
Sbjct: 957 VEERDPPSPLLQML--ENNSKSVIGENWTTCLHSSLVDNLGKYRKYDGSKILDILRVLRN 1014
Query: 346 NR 351
R
Sbjct: 1015 KR 1016
>SPAC688.10 |rev3||DNA polymerase zeta catalytic subunit
Rev3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1480
Score = 30.7 bits (66), Expect = 0.20
Identities = 15/42 (35%), Positives = 23/42 (54%)
Frame = +1
Query: 556 ESMPYKVDPKSGLIDYDKLAETAKLFKPRLIIAGMSCYSRCL 681
E++P ++PKS L + + + P +IIA CYS CL
Sbjct: 934 EALPLVMEPKSDLYNNPVVVLDFQSLYPSIIIAYNLCYSTCL 975
>SPBC32H8.13c |mok12||alpha-1,3-glucan synthase
Mok12|Schizosaccharomyces pombe|chr 2|||Manual
Length = 2352
Score = 28.7 bits (61), Expect = 0.83
Identities = 20/74 (27%), Positives = 37/74 (50%), Gaps = 3/74 (4%)
Frame = +1
Query: 241 QCLSSCLH-NKYSEGMPNQRYYG-GNEY-IDEIEILAQNRSLEAYRLKSEEWGVNVQPYS 411
+C+S + + ++ + +Q Y G N++ I E+ + N+S +A + +G PYS
Sbjct: 1762 ECVSKDIEVDNFALNLQSQSYEGDSNDFGIREVPLSDANQSSQADSTSIDRYG----PYS 1817
Query: 412 GSPANFAVYTGIVE 453
NF+ Y VE
Sbjct: 1818 SQKVNFSKYKDFVE 1831
>SPAC4A8.12c |sds22||protein phosphatase regulatory subunit Sds22
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 332
Score = 27.5 bits (58), Expect = 1.9
Identities = 18/54 (33%), Positives = 25/54 (46%), Gaps = 2/54 (3%)
Frame = +1
Query: 184 QRAGLEMIASENFTSVPVLQCLSSCLHNKY--SEGMPNQRYYGGNEYIDEIEIL 339
Q+ L I S T L CLS CL Y G+ + + G E ++ +EIL
Sbjct: 192 QKLSLLSIQSNRITQFENLACLSHCLRELYVSHNGLTS---FSGIEVLENLEIL 242
>SPBC577.06c |||phosphatidylinositol kinase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1877
Score = 27.5 bits (58), Expect = 1.9
Identities = 18/55 (32%), Positives = 29/55 (52%), Gaps = 1/55 (1%)
Frame = +1
Query: 415 SPANFAVYTGIVEPHGRIMG-LDLPDGGHLTHGFFTATKKISATSIFFESMPYKV 576
S A ++V T +++ R G + + D GH+ H F I+ I FES P+K+
Sbjct: 1716 SMAAYSVITYLLQFKDRHNGNIMIDDQGHILHIDFGFIFDIAPGGITFESAPFKL 1770
>SPAC16A10.01 |||DUF1212 family protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 830
Score = 26.6 bits (56), Expect = 3.3
Identities = 16/43 (37%), Positives = 23/43 (53%), Gaps = 2/43 (4%)
Frame = -3
Query: 626 FAVSASLS*SINPLLGSTLYGMLSKNIDVAD--IFLVAVKKPW 504
+AV SL S +GS LYG + K+ AD + ++AV W
Sbjct: 624 YAVIFSLFLSFGITIGSALYGWMDKDATDADTCMSIIAVSPYW 666
>SPAC1F3.05 |||adaptin |Schizosaccharomyces pombe|chr 1|||Manual
Length = 510
Score = 26.6 bits (56), Expect = 3.3
Identities = 15/35 (42%), Positives = 21/35 (60%)
Frame = -3
Query: 209 AIISSPAR*RSFSLTIISKSSGSASQRLLFSNLAL 105
+I+ S + S LT + KSSG AS + FSN +L
Sbjct: 397 SILQSDSLMISTQLTSVQKSSGFASYSVQFSNCSL 431
>SPAC30.01c |sec72|sec7b|Sec7 domain|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1822
Score = 26.2 bits (55), Expect = 4.4
Identities = 14/55 (25%), Positives = 32/55 (58%)
Frame = -1
Query: 379 PILICMLPETCSVPVSLFHQYIHSPHSTVDWACLQNICYEDMNSGTEVQELM*NF 215
P++ +L ++P+ LF + IH T+ ++C I E+++ +++EL+ N+
Sbjct: 1758 PVIATILDSILNLPLELFSENIH----TLYFSCCSMIAKENLDD--QLRELLKNY 1806
>SPAC869.08 |pcm2||protein-L-isoaspartate O-methyltransferase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 230
Score = 25.8 bits (54), Expect = 5.8
Identities = 12/49 (24%), Positives = 22/49 (44%)
Frame = +1
Query: 313 EYIDEIEILAQNRSLEAYRLKSEEWGVNVQPYSGSPANFAVYTGIVEPH 459
+++ E + L R+++A S + + PY SP + I PH
Sbjct: 15 QHLVESKFLTNQRAIKAMNATSRSFYCPLSPYMDSPQSIGYGVTISAPH 63
>SPBC11G11.07 ||SPBC18H10.01|karyopherin|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 955
Score = 25.8 bits (54), Expect = 5.8
Identities = 14/41 (34%), Positives = 22/41 (53%)
Frame = -3
Query: 560 LSKNIDVADIFLVAVKKPWVRCPPSGKSNPIILPWGSTMPV 438
L+ NID+ IFL V P P+G+ +PI+ + P+
Sbjct: 620 LADNIDIVTIFLSLVHPP----SPAGELHPIVRLFQDIWPI 656
>SPAC20H4.10 |ufd2||ubiquitin-protein ligase E4 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1010
Score = 25.4 bits (53), Expect = 7.7
Identities = 15/58 (25%), Positives = 29/58 (50%)
Frame = +1
Query: 103 MSAKLLNSNLWEADPELFDIIVKEKDRQRAGLEMIASENFTSVPVLQCLSSCLHNKYS 276
++ + LN L E D + + +KD + G ++ +EN +L LS+ +N +S
Sbjct: 84 ITCQALNITLSETDSSKYYLEGFKKDLEEEGSPLLFNENNVDSALLSRLSTTGNNTFS 141
>SPAC3F10.17 |||ribosome biogenesis protein Ltv1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 386
Score = 25.4 bits (53), Expect = 7.7
Identities = 8/20 (40%), Positives = 13/20 (65%)
Frame = +1
Query: 379 EEWGVNVQPYSGSPANFAVY 438
EE+G ++P G AN+ +Y
Sbjct: 57 EEYGSTIRPNEGEAANYGIY 76
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,920,815
Number of Sequences: 5004
Number of extensions: 63865
Number of successful extensions: 198
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 195
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 198
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 315915086
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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