Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmnc20o20
(626 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC11E10.07c |||translation initiation factor eIF2B alpha subun... 131 9e-32
SPAC21E11.06 |tif224||translation initiation factor eIF2B delta ... 61 1e-10
SPBC23E6.10c |||methylthioribose-1-phosphate isomerase |Schizosa... 35 0.008
SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual 35 0.011
SPCC584.01c |||sulfite reductase NADPH flavoprotein subunit |Sch... 31 0.18
SPAP11E10.02c |mam3|SPAPB1A10.01c|cell agglutination protein Mam... 28 1.3
SPAC10F6.01c ||SPAC4C5.05c|sulfite reductase beta subunit |Schiz... 27 2.9
SPBC1685.07c |||amino acid transporter |Schizosaccharomyces pomb... 26 5.1
SPBC11G11.07 ||SPBC18H10.01|karyopherin|Schizosaccharomyces pomb... 25 6.8
SPBC20F10.07 |||GRAM domain protein|Schizosaccharomyces pombe|ch... 25 6.8
SPBC27B12.08 |||AP-1 accessory protein |Schizosaccharomyces pomb... 25 9.0
SPBC16A3.01 |spn3|SPBC543.01c|septin Spn3|Schizosaccharomyces po... 25 9.0
SPAC23H3.05c |swd1||COMPASS complex subunit Swd1|Schizosaccharom... 25 9.0
>SPCC11E10.07c |||translation initiation factor eIF2B alpha
subunit|Schizosaccharomyces pombe|chr 3|||Manual
Length = 341
Score = 131 bits (316), Expect = 9e-32
Identities = 63/195 (32%), Positives = 115/195 (58%)
Frame = +3
Query: 42 EVQAIFMKILREEEDVSAGVAAIKTLLTVIENYKVSTVRELDLNLQLAVDAMRHCDQPVT 221
++ ++ K L+++ +++ VAAI+ L+ ++ + T+ E LQ + ++ Q
Sbjct: 18 DIVQVYKKFLQDDPEITMPVAAIEALVQLLSRSQAKTISEFMDILQNGSNTLKEGVQNNI 77
Query: 222 AISSGCELFMRFITFAKLDTKSFEECEKIMLQRGNIFLDKLLEARGKVAKQALSFICDGC 401
++S+GC++F RF+T + D FE+C++ +++ G +F+ + R ++A I DG
Sbjct: 78 SLSAGCDIFQRFVTRSLHDVGDFEQCKRHLVENGKLFIQRARACRQRIAHLGYPLIRDGS 137
Query: 402 KILTHSRSRVVLQAMIEAIKANKRFEVFITVSAPDNSGEMMHKQLVAAGVDATLILDSAV 581
ILTH SR V ++ A K + RF+VF+T S P SG +M + L A + ++LDSAV
Sbjct: 138 VILTHGFSRGVAAVLLAAAKRHVRFKVFVTESRPSGSGCLMTRTLKNACIPTCMVLDSAV 197
Query: 582 GYVMEQVDIVMIGAE 626
+ M +VD+V++GAE
Sbjct: 198 SFTMNRVDLVLVGAE 212
>SPAC21E11.06 |tif224||translation initiation factor eIF2B delta
subunit|Schizosaccharomyces pombe|chr 1|||Manual
Length = 467
Score = 61.3 bits (142), Expect = 1e-10
Identities = 43/175 (24%), Positives = 82/175 (46%), Gaps = 4/175 (2%)
Frame = +3
Query: 108 IKTLLTVIENYKVSTVRELDLNLQLAVD---AMRHCDQPVTAISSGCELFMRF-ITFAKL 275
+KT VI++Y+ L +L ++ A +P++ F++ I+ +
Sbjct: 168 LKTFKIVIQDYQTPYGTTLSRHLTTHINSQIAYLVSTRPLSISMGNAIRFLKLEISVLDI 227
Query: 276 DTKSFEECEKIMLQRGNIFLDKLLEARGKVAKQALSFICDGCKILTHSRSRVVLQAMIEA 455
D E E ++ + + D+++ A + + A I DG ILT+ S V +I A
Sbjct: 228 DLTDDEGKELLLEKIDSYIRDRIIIAGQVIVQAATEKIQDGDVILTYLHSSTVNDVLIHA 287
Query: 456 IKANKRFEVFITVSAPDNSGEMMHKQLVAAGVDATLILDSAVGYVMEQVDIVMIG 620
K+F V + S P+ G + K L G++ T ++ SA+ Y+M++V + +G
Sbjct: 288 KNVGKKFRVVVVDSRPEFEGRVCLKLLTEHGIECTYVMISALSYIMQEVTKIFLG 342
>SPBC23E6.10c |||methylthioribose-1-phosphate isomerase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 359
Score = 35.1 bits (77), Expect = 0.008
Identities = 16/49 (32%), Positives = 29/49 (59%), Gaps = 1/49 (2%)
Frame = +3
Query: 483 FITVSAPDNSGEMMHK-QLVAAGVDATLILDSAVGYVMEQVDIVMIGAE 626
+ T + P N G + +LV + ATL+ DS V +M ++D +++GA+
Sbjct: 187 YCTETRPYNQGSRLTAFELVHDKIPATLVTDSTVASIMHKIDAIVVGAD 235
>SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual
Length = 1236
Score = 34.7 bits (76), Expect = 0.011
Identities = 39/188 (20%), Positives = 85/188 (45%), Gaps = 7/188 (3%)
Frame = -2
Query: 580 TAESKISVASTPAATSCLCIISPLLSGADTVMKT-------SNLLFAFIASIIACKTTLD 422
++ S I + TP+ +S + S ++SG+ +++ + S+ L + +S+I +TL
Sbjct: 619 SSSSTILSSPTPSTSSLMISSSSIISGSSSILSSSISTIPISSSLSTYSSSVIPSSSTLV 678
Query: 421 RECVKILHPSQMNDKACFATFPRASNSLSRNILPLCNIIFSHSSKLFVSNLANVINLINN 242
++ S + + P +S+ +S L NI S S +S+ + + +N+
Sbjct: 679 SSSSSLIVSSSPVASSSSSPIPSSSSLVSTYSASLSNITHSSLSLTAMSSSSAIPTSVNS 738
Query: 241 SHPDEIAVTG*SQCLIASTANWRFRSNSLTVDTL*FSITVNKVLMAATPAETSSSSLRIF 62
S + S L++S + +S TV + S + ++ T+SS+L
Sbjct: 739 S---TLITASSSNTLLSSITSSSAIVSSTTVSNI--SSNLPSATASSQSQLTNSSTLATS 793
Query: 61 MNIACTSS 38
+ ++ +SS
Sbjct: 794 LYLSSSSS 801
>SPCC584.01c |||sulfite reductase NADPH flavoprotein subunit
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1006
Score = 30.7 bits (66), Expect = 0.18
Identities = 21/72 (29%), Positives = 33/72 (45%)
Frame = -2
Query: 577 AESKISVASTPAATSCLCIISPLLSGADTVMKTSNLLFAFIASIIACKTTLDRECVKILH 398
A S V S +SCL +++PLLS S L +A++ + T + V +L
Sbjct: 82 AISSPGVYSMMTPSSCLSLLNPLLSNIIPFYGASKPLVVHVAALAYLEQTYCADNVSVLD 141
Query: 397 PSQMNDKACFAT 362
S N+ FA+
Sbjct: 142 FSYANNFTVFAS 153
>SPAP11E10.02c |mam3|SPAPB1A10.01c|cell agglutination protein
Mam3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1082
Score = 27.9 bits (59), Expect = 1.3
Identities = 17/45 (37%), Positives = 27/45 (60%)
Frame = -2
Query: 610 TISTCSMT*PTAESKISVASTPAATSCLCIISPLLSGADTVMKTS 476
++ST S T PT+ S +S+ + +S L I SP++S + T TS
Sbjct: 536 SLSTPSTTIPTSNSSVSLQT----SSSLIISSPIISSSLTATSTS 576
>SPAC10F6.01c ||SPAC4C5.05c|sulfite reductase beta subunit
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1473
Score = 26.6 bits (56), Expect = 2.9
Identities = 19/88 (21%), Positives = 36/88 (40%), Gaps = 1/88 (1%)
Frame = +3
Query: 366 AKQALSFICDGCKILTHSRSRVVLQAMIEAIKANKRFEVF-ITVSAPDNSGEMMHKQLVA 542
A ++F C + H + ++ A+ E++K K+FE ++ P H A
Sbjct: 13 AVSRIAFRCSDALYVVHPNNSILNGALTESLKDLKKFETLNVSGKVPHVLPLKSHADPFA 72
Query: 543 AGVDATLILDSAVGYVMEQVDIVMIGAE 626
DA L + +Q+ V+ A+
Sbjct: 73 HIADAILAEEEVASTQPKQITSVVASAD 100
>SPBC1685.07c |||amino acid transporter |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 420
Score = 25.8 bits (54), Expect = 5.1
Identities = 9/30 (30%), Positives = 20/30 (66%)
Frame = -2
Query: 328 ILPLCNIIFSHSSKLFVSNLANVINLINNS 239
++ LC ++F+HS L +S+L V+ + ++
Sbjct: 328 VITLCILLFTHSLALLLSSLEMVLAFVGST 357
>SPBC11G11.07 ||SPBC18H10.01|karyopherin|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 955
Score = 25.4 bits (53), Expect = 6.8
Identities = 12/38 (31%), Positives = 23/38 (60%), Gaps = 1/38 (2%)
Frame = -2
Query: 364 TFPRASNS-LSRNILPLCNIIFSHSSKLFVSNLANVIN 254
TFPR + S S ++PL ++F+ L + ++NV++
Sbjct: 861 TFPRDNISDASAVLIPLIRLVFAADPSLCIKYMSNVLD 898
>SPBC20F10.07 |||GRAM domain protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 764
Score = 25.4 bits (53), Expect = 6.8
Identities = 13/25 (52%), Positives = 16/25 (64%), Gaps = 1/25 (4%)
Frame = +3
Query: 78 EEDVSAGVAA-IKTLLTVIENYKVS 149
E+ G A+ +K LLT ENYKVS
Sbjct: 572 EKGAQEGQASYVKDLLTAFENYKVS 596
>SPBC27B12.08 |||AP-1 accessory protein |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1919
Score = 25.0 bits (52), Expect = 9.0
Identities = 10/38 (26%), Positives = 20/38 (52%)
Frame = -1
Query: 119 QSFDGSHTS*DILFFSQDLHEYSLYFFFFHVLNLVFTC 6
+ +G H +L+F +HE Y +VL+++ +C
Sbjct: 81 KGLEGYHQHRVLLYFVDSIHEKKDYIEIINVLSVLTSC 118
>SPBC16A3.01 |spn3|SPBC543.01c|septin Spn3|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 412
Score = 25.0 bits (52), Expect = 9.0
Identities = 11/30 (36%), Positives = 19/30 (63%)
Frame = +2
Query: 302 KNYVTKRQYISGQAVRSTRKSSKTGFVIHL 391
+ ++TK Q +R+T+KSSK G ++L
Sbjct: 26 RTHITKGQIDIELTMRTTKKSSKKGIPLNL 55
>SPAC23H3.05c |swd1||COMPASS complex subunit
Swd1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 398
Score = 25.0 bits (52), Expect = 9.0
Identities = 14/32 (43%), Positives = 17/32 (53%)
Frame = -1
Query: 227 DSCNRLITMSHCIHSQLEIQI*FSHCRYFIIF 132
D+ I SH I SQ QI S C+ F+IF
Sbjct: 193 DASTTKIRSSHRITSQSIKQIRLSFCKRFLIF 224
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,455,399
Number of Sequences: 5004
Number of extensions: 48126
Number of successful extensions: 113
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 110
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 113
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 277683324
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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