BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmnc20m05
(670 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC3H1.11 |hsr1||transcription factor Hsr1|Schizosaccharomyces ... 28 1.1
SPBC25H2.06c |hrf1||COPII-coated vesicle component Hrf1 |Schizos... 28 1.4
SPAC4F8.08 |mug114||sequence orphan|Schizosaccharomyces pombe|ch... 28 1.4
SPAC22F3.12c |rgs1||regulator of G-protein signaling Rgs1|Schizo... 27 2.4
SPAC8E11.01c ||SPAC959.01|beta-fructofuranosidase|Schizosaccharo... 26 4.3
SPAC2F7.10 |||palmitoyltransferase |Schizosaccharomyces pombe|ch... 25 7.5
SPBC17D11.07c |rpn2||19S proteasome regulatory subunit Rpn2|Schi... 25 7.5
SPBC14F5.07 |||ER-localized ubiquitin ligase |Schizosaccharomyce... 25 7.5
SPAC1782.08c |rex3||exonuclease Rex3 |Schizosaccharomyces pombe|... 25 7.5
>SPAC3H1.11 |hsr1||transcription factor Hsr1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 582
Score = 28.3 bits (60), Expect = 1.1
Identities = 13/33 (39%), Positives = 17/33 (51%)
Frame = -3
Query: 524 RDCIYRRVSLHKNWLPRPWCYFQPSPWLPQRCP 426
RD + + LH N PR C+FQPS + P
Sbjct: 463 RDNLRQHERLHVNASPRLACFFQPSGYYSSGAP 495
>SPBC25H2.06c |hrf1||COPII-coated vesicle component Hrf1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 293
Score = 27.9 bits (59), Expect = 1.4
Identities = 19/72 (26%), Positives = 35/72 (48%), Gaps = 2/72 (2%)
Frame = -2
Query: 651 YSLVISLDTMF*TTVPFTSMKLANDLVTDASRV--LPFVSRITITRLYLSSCFFT*ELAS 478
Y L IS + + F+ K ++T S++ +P+V+R +YL++ FF L S
Sbjct: 192 YLLNISSQSQVLDLLAFSGYKFVGLILTSLSKLFEMPWVTRFVFLYMYLATAFFL--LRS 249
Query: 477 QTLVLFPTFSLA 442
+ P ++A
Sbjct: 250 LKYAVLPESTMA 261
>SPAC4F8.08 |mug114||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 151
Score = 27.9 bits (59), Expect = 1.4
Identities = 12/27 (44%), Positives = 18/27 (66%)
Frame = +2
Query: 419 KEKGIFVVAKEKVGNNTRVWEASSYVK 499
K+ G+F +AK K+ N T+VW + Y K
Sbjct: 4 KKGGLFKIAK-KLRNGTKVWARAGYFK 29
>SPAC22F3.12c |rgs1||regulator of G-protein signaling
Rgs1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 481
Score = 27.1 bits (57), Expect = 2.4
Identities = 19/64 (29%), Positives = 28/64 (43%), Gaps = 2/64 (3%)
Frame = +1
Query: 268 TVPLCSG-CWCGPLRTAMGLSL-SIPSIKTGSNHLCVIIFHTDGYFDPLHYIQREGHLCG 441
T+P + C C A L + + PS + SN C++ GY ++Q GH
Sbjct: 115 TIPKTAAKCLCNTFLNARLLQIVNNPSARKFSNEKCLLQLTRKGYSVVSEFLQHNGH--N 172
Query: 442 SQGE 453
SQ E
Sbjct: 173 SQAE 176
>SPAC8E11.01c
||SPAC959.01|beta-fructofuranosidase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 508
Score = 26.2 bits (55), Expect = 4.3
Identities = 10/24 (41%), Positives = 15/24 (62%), Gaps = 1/24 (4%)
Frame = +3
Query: 285 WLLVWPFTHCYGIIFIHSLN-QDW 353
W++V Y ++F HSLN +DW
Sbjct: 162 WIMVVVLAQKYKVLFYHSLNLRDW 185
>SPAC2F7.10 |||palmitoyltransferase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 642
Score = 25.4 bits (53), Expect = 7.5
Identities = 9/23 (39%), Positives = 14/23 (60%)
Frame = -1
Query: 520 IVFIVVFLYIRTGFPDPGVISNL 452
I F + LY+RT F +PG + +
Sbjct: 355 ISFTCIGLYVRTAFQNPGYVDKI 377
>SPBC17D11.07c |rpn2||19S proteasome regulatory subunit
Rpn2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 965
Score = 25.4 bits (53), Expect = 7.5
Identities = 8/15 (53%), Positives = 12/15 (80%)
Frame = +1
Query: 523 RNCYARHEWQHS*GI 567
+ CYA++EW+H GI
Sbjct: 147 QKCYAKNEWRHVLGI 161
>SPBC14F5.07 |||ER-localized ubiquitin ligase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1242
Score = 25.4 bits (53), Expect = 7.5
Identities = 17/64 (26%), Positives = 26/64 (40%)
Frame = +2
Query: 401 TLYTTFKEKGIFVVAKEKVGNNTRVWEASSYVKKHDDKYNLVIVMRDTNGNTREASVTKS 580
T++ K +GIF + ++V NN W D Y +I T V +S
Sbjct: 527 TVFVKLKLQGIFSSSFQQVSNNMYSWIYDHVFSSSDHAYESLIYYMKTG----HKQVVQS 582
Query: 581 FANF 592
F+ F
Sbjct: 583 FSIF 586
>SPAC1782.08c |rex3||exonuclease Rex3 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 540
Score = 25.4 bits (53), Expect = 7.5
Identities = 10/19 (52%), Positives = 14/19 (73%)
Frame = +1
Query: 502 TRR*IQSRNCYARHEWQHS 558
T R QSRNC+ H++Q+S
Sbjct: 18 TGRKCQSRNCFFSHDFQNS 36
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,647,799
Number of Sequences: 5004
Number of extensions: 53237
Number of successful extensions: 141
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 136
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 141
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 305854096
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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