Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= bmnc20d22
         (334 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC13B11.04c ||SPCC777.01c|glutathione-dependent formaldehyde d...    94   6e-21
SPBC1539.07c |||glutathione-dependent formaldehyde dehydrogenase...    84   5e-18
SPCC162.04c |wtf13||wtf element Wtf13|Schizosaccharomyces pombe|...    27   0.57 
SPBC27B12.08 |||AP-1 accessory protein |Schizosaccharomyces pomb...    25   2.3  
SPAC1952.17c ||SPAC890.01c|GTPase activating protein|Schizosacch...    25   2.3  
SPAC1F5.11c |||phosphatidylinositol kinase |Schizosaccharomyces ...    25   2.3  
SPBC16D10.05 |mok13||alpha-1,3-glucan synthase Mok13|Schizosacch...    25   4.0  
SPBC14F5.08 |med7||mediator complex subunit Med7|Schizosaccharom...    24   5.3  
SPBC17A3.10 |pas4||peroxisomal ubiquitin-protein ligase E3 |Schi...    24   7.0  
SPCC1183.04c |pet127||mitochondrial membrane protein Pet127|Schi...    24   7.0  
SPBC19C7.08c |||leucine carboxyl methyltransferase|Schizosacchar...    24   7.0  
SPAC1805.05 |cki3||serine/threonine protein kinase Cki3|Schizosa...    23   9.2  
SPAC30.01c |sec72|sec7b|Sec7 domain|Schizosaccharomyces pombe|ch...    23   9.2  

>SPCC13B11.04c ||SPCC777.01c|glutathione-dependent formaldehyde
           dehydrogenase|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 380

 Score = 93.9 bits (223), Expect = 6e-21
 Identities = 41/70 (58%), Positives = 51/70 (72%)
 Frame = +2

Query: 2   PFQLVTGRTWKGTAFGGYKSRESVPKLVDEYLEKKLPLDEFVTHNVPLKEINEAFHLMHA 181
           PFQLVTGR W+G AFGG K R  +P LV EYL+ KL +D+++TH  PLKEINEAF  MH 
Sbjct: 310 PFQLVTGRVWRGCAFGGVKGRSQLPDLVKEYLDHKLEIDKYITHRRPLKEINEAFTDMHN 369

Query: 182 GKSIRAVVDM 211
           G  I+ V+ +
Sbjct: 370 GNCIKTVLSI 379


>SPBC1539.07c |||glutathione-dependent formaldehyde dehydrogenase
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 378

 Score = 84.2 bits (199), Expect = 5e-18
 Identities = 38/70 (54%), Positives = 48/70 (68%)
 Frame = +2

Query: 2   PFQLVTGRTWKGTAFGGYKSRESVPKLVDEYLEKKLPLDEFVTHNVPLKEINEAFHLMHA 181
           PF +VTGR   G+AFGG K R  +P  VDEY++    +DE++T+  PLK IN+AF  MH 
Sbjct: 306 PFLVVTGRQVLGSAFGGVKGRSELPNFVDEYMQGHFKVDEYITNEEPLKNINKAFDHMHE 365

Query: 182 GKSIRAVVDM 211
           GK IR VVDM
Sbjct: 366 GKCIRCVVDM 375


>SPCC162.04c |wtf13||wtf element Wtf13|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 418

 Score = 27.5 bits (58), Expect = 0.57
 Identities = 16/52 (30%), Positives = 29/52 (55%), Gaps = 3/52 (5%)
 Frame = -1

Query: 331 FFYCVNKMH--VVYLNYT-CSKC*RKKV*QVYKNSINIFP*KLHVNYCTDRF 185
           FFY +  ++  V+Y N+T  S+C +K   Q+Y+   + FP  ++   C + F
Sbjct: 315 FFYEMGSLYIPVIYSNHTRTSRCSQKDA-QIYREWYSFFPRYVYSTNCMNDF 365


>SPBC27B12.08 |||AP-1 accessory protein |Schizosaccharomyces pombe|chr
            2|||Manual
          Length = 1919

 Score = 25.4 bits (53), Expect = 2.3
 Identities = 11/25 (44%), Positives = 14/25 (56%)
 Frame = +2

Query: 230  IYTIFVHLLNLFSSAFTTSIIKIHN 304
            IYT+F HLLN++      S  K  N
Sbjct: 1720 IYTLFEHLLNIYKETINDSDKKEKN 1744


>SPAC1952.17c ||SPAC890.01c|GTPase activating
           protein|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 619

 Score = 25.4 bits (53), Expect = 2.3
 Identities = 12/30 (40%), Positives = 17/30 (56%)
 Frame = +2

Query: 146 KEINEAFHLMHAGKSIRAVVDM*FLRKNIY 235
           K +N  F L H  K+ +   DM ++RKN Y
Sbjct: 444 KLLNLTFELQHLRKTSKNDEDMSYVRKNSY 473


>SPAC1F5.11c |||phosphatidylinositol kinase |Schizosaccharomyces
            pombe|chr 1|||Manual
          Length = 3655

 Score = 25.4 bits (53), Expect = 2.3
 Identities = 12/29 (41%), Positives = 17/29 (58%), Gaps = 1/29 (3%)
 Frame = -3

Query: 104  SSPSTHLQALVH-FLYFCNLQKLFPSRCD 21
            SSPS+ L+     F Y CN+ KL+  + D
Sbjct: 914  SSPSSDLEVKQQAFTYVCNISKLYVYKSD 942


>SPBC16D10.05 |mok13||alpha-1,3-glucan synthase
            Mok13|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 2358

 Score = 24.6 bits (51), Expect = 4.0
 Identities = 8/35 (22%), Positives = 19/35 (54%)
 Frame = +2

Query: 101  KKLPLDEFVTHNVPLKEINEAFHLMHAGKSIRAVV 205
            K+ P   F+T  + + ++   +HL+  G S  +++
Sbjct: 1026 KEAPPSNFLTWEIIISDLTRTYHLIPRGSSTVSII 1060


>SPBC14F5.08 |med7||mediator complex subunit
           Med7|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 376

 Score = 24.2 bits (50), Expect = 5.3
 Identities = 21/86 (24%), Positives = 35/86 (40%), Gaps = 9/86 (10%)
 Frame = +2

Query: 77  KLVDEYLEKKLPLDEFVTHNVPLKEINEAFHLMHAGKS---------IRAVVDM*FLRKN 229
           K  +E ++ K PLD   T  V    +NE F      K           R   ++ FL ++
Sbjct: 175 KKEEEDIQMKEPLDSQDTGAVSASSVNEGFRADQKSKDGETSDLIKIPRRAYELRFLSRS 234

Query: 230 IYTIFVHLLNLFSSAFTTSIIKIHNM 307
           +   F+ LL + + A      K+ N+
Sbjct: 235 LMLNFLELLGIMAKAPEQFPSKVENI 260


>SPBC17A3.10 |pas4||peroxisomal ubiquitin-protein ligase E3
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 306

 Score = 23.8 bits (49), Expect = 7.0
 Identities = 10/22 (45%), Positives = 12/22 (54%)
 Frame = +1

Query: 211 VISKEKYLYYFCTLVKPFFFSI 276
           VISKEK+L Y     KP    +
Sbjct: 141 VISKEKHLVYILNSFKPILLKL 162


>SPCC1183.04c |pet127||mitochondrial membrane protein
           Pet127|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 524

 Score = 23.8 bits (49), Expect = 7.0
 Identities = 9/13 (69%), Positives = 11/13 (84%)
 Frame = +2

Query: 86  DEYLEKKLPLDEF 124
           DEYL K LP+D+F
Sbjct: 87  DEYLSKILPVDKF 99


>SPBC19C7.08c |||leucine carboxyl
           methyltransferase|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 681

 Score = 23.8 bits (49), Expect = 7.0
 Identities = 11/23 (47%), Positives = 14/23 (60%)
 Frame = -2

Query: 222 LRNYMSTTARIDFPACIKWNASL 154
           LRN + +  R D PAC+K   SL
Sbjct: 355 LRNSIYSLKRTDLPACLKELPSL 377


>SPAC1805.05 |cki3||serine/threonine protein kinase
           Cki3|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 439

 Score = 23.4 bits (48), Expect = 9.2
 Identities = 12/20 (60%), Positives = 16/20 (80%), Gaps = 1/20 (5%)
 Frame = +2

Query: 53  YKSRES-VPKLVDEYLEKKL 109
           ++SR+S VP+L DEYL  KL
Sbjct: 45  FESRKSEVPQLRDEYLTYKL 64


>SPAC30.01c |sec72|sec7b|Sec7 domain|Schizosaccharomyces pombe|chr
            1|||Manual
          Length = 1822

 Score = 23.4 bits (48), Expect = 9.2
 Identities = 8/21 (38%), Positives = 14/21 (66%)
 Frame = -1

Query: 208  VNYCTDRFSSMHQMECLIDLL 146
            VN  ++ + ++ Q  CLID+L
Sbjct: 1330 VNLFSEHYDTLMQQNCLIDML 1350


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,454,791
Number of Sequences: 5004
Number of extensions: 28096
Number of successful extensions: 66
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 64
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 66
length of database: 2,362,478
effective HSP length: 64
effective length of database: 2,042,222
effective search space used: 93942212
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)