BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmnc20d20
(565 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC887.01 |||acireductone dioxygenase family|Schizosaccharomyce... 125 4e-30
SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 27 1.9
SPAC25G10.07c |cut7||kinesin-like protein Cut7|Schizosaccharomyc... 27 2.5
SPBC582.07c |rpn7||19S proteasome regulatory subunit Rpn7|Schizo... 26 3.3
SPAC19G12.16c |adg2|SPAC23A1.01c, mug46|conserved fungal protein... 26 4.4
SPBC20F10.02c |||DUF1741 family protein|Schizosaccharomyces pomb... 25 7.7
SPAC12B10.01c ||SPAC31F12.02c, SPAC637.15c|ubiquitin-protein lig... 25 7.7
SPBC12D12.04c |pck2|sts6, pkc1|protein kinase C |Schizosaccharom... 25 7.7
SPCP1E11.11 |||Puf family RNA-binding protein|Schizosaccharomyce... 25 7.7
>SPBC887.01 |||acireductone dioxygenase family|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 178
Score = 125 bits (302), Expect = 4e-30
Identities = 61/138 (44%), Positives = 91/138 (65%), Gaps = 2/138 (1%)
Frame = +1
Query: 145 ISLDDL-FSKTGVEYFKINIDTYKTDGVLEKLKNERGYTYEDEMVCSKECLPN-YEEKIK 318
+S +DL +K + ++ T+ DG+++ E G+ DE+V S++ L + Y+ +K
Sbjct: 22 VSAEDLEAAKVSYRHHDGDLHTF-ADGLMK----EYGFKNRDEVVVSRKGLGDRYDNMVK 76
Query: 319 SFYTEHLHTDEEIRFILDGSGYFDVRDGEDQWIRIAVSKGDMLVIPSGIYHRFTLDTNNY 498
F+ EHLH DEEIR ILDG+GYFDVR +D+W+RI V KGD++++P GIYHRFT T++Y
Sbjct: 77 KFFEEHLHEDEEIRLILDGNGYFDVRSVDDRWVRIFVEKGDLIILPPGIYHRFTTTTDDY 136
Query: 499 IKAKRFFIGEPVWLPYNR 552
I A R F P W+ +R
Sbjct: 137 IHAMRLFHENPKWIALSR 154
>SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 800
Score = 27.1 bits (57), Expect = 1.9
Identities = 11/29 (37%), Positives = 17/29 (58%)
Frame = +2
Query: 410 NGSVLPCPRETCWSSRAVSTTGSLWTPIT 496
N S LP +C +S ++ TG+ TP+T
Sbjct: 274 NSSPLPTTSTSCTTSTSIPPTGNSTTPVT 302
Score = 25.0 bits (52), Expect = 7.7
Identities = 10/29 (34%), Positives = 17/29 (58%)
Frame = +2
Query: 410 NGSVLPCPRETCWSSRAVSTTGSLWTPIT 496
+ S LP +C +S ++ TG+ TP+T
Sbjct: 328 SSSPLPSTSTSCTTSTSIPPTGNSTTPVT 356
>SPAC25G10.07c |cut7||kinesin-like protein Cut7|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1085
Score = 26.6 bits (56), Expect = 2.5
Identities = 19/69 (27%), Positives = 30/69 (43%)
Frame = +1
Query: 118 QHHKNPPEYISLDDLFSKTGVEYFKINIDTYKTDGVLEKLKNERGYTYEDEMVCSKECLP 297
Q HK E + L + S T +E K + K + VLE K ++ T E ++ L
Sbjct: 510 QEHKKEVEALQLQLVNSSTELESVKSENEKLKNELVLEIEKRKKYETNEAKITTVATDLS 569
Query: 298 NYEEKIKSF 324
Y + K +
Sbjct: 570 QYYRESKEY 578
>SPBC582.07c |rpn7||19S proteasome regulatory subunit
Rpn7|Schizosaccharomyces pombe|chr 2|||Manual
Length = 409
Score = 26.2 bits (55), Expect = 3.3
Identities = 13/35 (37%), Positives = 20/35 (57%)
Frame = -3
Query: 248 RSFLSFSSTPSVLYVSMFILKYSTPVLENKSSREI 144
R+ + T S L +S I + S+P LENKS + +
Sbjct: 11 RTMTEKARTVSDLTISQAIFELSSPFLENKSQKAL 45
>SPAC19G12.16c |adg2|SPAC23A1.01c, mug46|conserved fungal
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 670
Score = 25.8 bits (54), Expect = 4.4
Identities = 23/90 (25%), Positives = 42/90 (46%)
Frame = -3
Query: 275 TISSSYV*PRSFLSFSSTPSVLYVSMFILKYSTPVLENKSSREIYSGGFLWCCSRWSVVS 96
++SSS V P S ++ +STP + VS L TP S + + G +V S
Sbjct: 238 SLSSSSVLPTSIITSTSTP--VTVSSSSLSSFTP----SYSTNLTTTGSTTTTGSATVSS 291
Query: 95 LSMYHACTILKTKIIN*ICTYVNNLSHYPT 6
Y +++ T + + + ++ ++ S Y T
Sbjct: 292 SPFYSNSSVIPTSVPSSVSSFTSSSSSYTT 321
>SPBC20F10.02c |||DUF1741 family protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 600
Score = 25.0 bits (52), Expect = 7.7
Identities = 13/31 (41%), Positives = 17/31 (54%)
Frame = -2
Query: 204 VYVYFKIFNPRFRE*IIQRNIFWRIFMVLQP 112
V+ Y + N + E I NIFW F +LQP
Sbjct: 14 VFYYKCLLNKNWNEPI--NNIFWGEFFLLQP 42
>SPAC12B10.01c ||SPAC31F12.02c, SPAC637.15c|ubiquitin-protein ligase
E3 |Schizosaccharomyces pombe|chr 1|||Manual
Length = 1647
Score = 25.0 bits (52), Expect = 7.7
Identities = 15/62 (24%), Positives = 29/62 (46%)
Frame = +1
Query: 142 YISLDDLFSKTGVEYFKINIDTYKTDGVLEKLKNERGYTYEDEMVCSKECLPNYEEKIKS 321
Y + D+ + ++F+ NI+ TD KL + E++ + CLP++ + S
Sbjct: 1164 YYGIRDVHTLFPDKHFRGNIENILTDFSNWKLSAKLNRQLEEQQLVVHGCLPSWCISLTS 1223
Query: 322 FY 327
Y
Sbjct: 1224 AY 1225
>SPBC12D12.04c |pck2|sts6, pkc1|protein kinase C
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1016
Score = 25.0 bits (52), Expect = 7.7
Identities = 13/32 (40%), Positives = 20/32 (62%)
Frame = -3
Query: 248 RSFLSFSSTPSVLYVSMFILKYSTPVLENKSS 153
+S SFSST S + ++ +L Y TP+ +K S
Sbjct: 91 KSAKSFSSTSSNIDSNLDLLNYDTPLTISKIS 122
>SPCP1E11.11 |||Puf family RNA-binding protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 642
Score = 25.0 bits (52), Expect = 7.7
Identities = 14/53 (26%), Positives = 25/53 (47%)
Frame = -3
Query: 299 FGRHSLEQTISSSYV*PRSFLSFSSTPSVLYVSMFILKYSTPVLENKSSREIY 141
FG +TI + S++ +P Y+++ I KY TP ++ E+Y
Sbjct: 185 FGSKQQRETICAELA--GSYVDLCKSPYGKYLAIKIFKYGTPKMKEVILGEMY 235
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,582,945
Number of Sequences: 5004
Number of extensions: 59179
Number of successful extensions: 172
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 167
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 172
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 238029836
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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