BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmnc1o08
(406 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g03850.1 68418.m00356 40S ribosomal protein S28 (RPS28B) ribo... 66 6e-12
At3g10090.1 68416.m01209 40S ribosomal protein S28 (RPS28A) simi... 66 6e-12
At5g64140.1 68418.m08054 40S ribosomal protein S28 (RPS28C) 65 1e-11
At2g43890.1 68415.m05456 polygalacturonase, putative / pectinase... 31 0.39
At4g35500.2 68417.m05045 protein kinase family protein contains ... 29 1.6
At4g35500.1 68417.m05044 protein kinase family protein contains ... 29 1.6
At4g34140.1 68417.m04845 D111/G-patch domain-containing protein ... 27 3.6
At1g29590.1 68414.m03618 eukaryotic translation initiation facto... 27 4.8
At1g29550.1 68414.m03614 eukaryotic translation initiation facto... 27 4.8
At1g29820.1 68414.m03645 expressed protein 27 6.3
At5g19460.1 68418.m02319 MutT/nudix family protein similar to SP... 26 8.3
At1g20260.2 68414.m02530 vacuolar ATP synthase subunit B, putati... 26 8.3
>At5g03850.1 68418.m00356 40S ribosomal protein S28 (RPS28B)
ribosomal protein S28, Arabidopsis thaliana,
EMBL:ATRP28A
Length = 64
Score = 66.5 bits (155), Expect = 6e-12
Identities = 34/53 (64%), Positives = 42/53 (79%)
Frame = +3
Query: 69 MDKPNVLARVVKVLGRTGSQGQCTQVKVEFIGETSRQIIRNVKGPVRDGDILT 227
MD A VVKV+GRTGS+GQ TQV+V+F ++ R I+RNVKGPVR+GDILT
Sbjct: 1 MDSQIKHAVVVKVMGRTGSRGQVTQVRVKFT-DSDRYIMRNVKGPVREGDILT 52
>At3g10090.1 68416.m01209 40S ribosomal protein S28 (RPS28A) similar
to ribosomal protein S28 GB:P34789 [Arabidopsis
thaliana]
Length = 64
Score = 66.5 bits (155), Expect = 6e-12
Identities = 34/53 (64%), Positives = 42/53 (79%)
Frame = +3
Query: 69 MDKPNVLARVVKVLGRTGSQGQCTQVKVEFIGETSRQIIRNVKGPVRDGDILT 227
MD A VVKV+GRTGS+GQ TQV+V+F ++ R I+RNVKGPVR+GDILT
Sbjct: 1 MDSQIKHAVVVKVMGRTGSRGQVTQVRVKFT-DSDRYIMRNVKGPVREGDILT 52
>At5g64140.1 68418.m08054 40S ribosomal protein S28 (RPS28C)
Length = 64
Score = 65.3 bits (152), Expect = 1e-11
Identities = 33/53 (62%), Positives = 42/53 (79%)
Frame = +3
Query: 69 MDKPNVLARVVKVLGRTGSQGQCTQVKVEFIGETSRQIIRNVKGPVRDGDILT 227
MD A VVKV+GRTGS+GQ TQV+V+F ++ R I+RNVKGPVR+GD+LT
Sbjct: 1 MDSQIKHAVVVKVMGRTGSRGQVTQVRVKFT-DSDRFIMRNVKGPVREGDVLT 52
>At2g43890.1 68415.m05456 polygalacturonase, putative / pectinase,
putative similar to SP|P48979 Polygalacturonase
precursor (EC 3.2.1.15) (PG) (Pectinase) {Prunus
persica}; contains Pfam profile PF00295: Glycosyl
hydrolases family 28 (polygalacturonases)
Length = 392
Score = 30.7 bits (66), Expect = 0.39
Identities = 14/32 (43%), Positives = 20/32 (62%)
Frame = -3
Query: 227 SEDVSVTDGSFHVSDDLTAGLPNELDLHLSTL 132
S V+VTDG+FH DD + P +L++S L
Sbjct: 204 SAGVTVTDGTFHTGDDCISIGPGTRNLYMSKL 235
>At4g35500.2 68417.m05045 protein kinase family protein contains
eukaryotic protein kinase domain, INTERPRO:IPR000719
Length = 439
Score = 28.7 bits (61), Expect = 1.6
Identities = 13/48 (27%), Positives = 26/48 (54%)
Frame = +3
Query: 45 LSSILQAKMDKPNVLARVVKVLGRTGSQGQCTQVKVEFIGETSRQIIR 188
LS+ +DK + R++ +G GQ + +EF+G++ ++IR
Sbjct: 87 LSAAADGDLDKTKCVVRLIDHFKHSGPNGQHLCMVLEFLGDSLLRLIR 134
>At4g35500.1 68417.m05044 protein kinase family protein contains
eukaryotic protein kinase domain, INTERPRO:IPR000719
Length = 438
Score = 28.7 bits (61), Expect = 1.6
Identities = 13/48 (27%), Positives = 26/48 (54%)
Frame = +3
Query: 45 LSSILQAKMDKPNVLARVVKVLGRTGSQGQCTQVKVEFIGETSRQIIR 188
LS+ +DK + R++ +G GQ + +EF+G++ ++IR
Sbjct: 86 LSAAADGDLDKTKCVVRLIDHFKHSGPNGQHLCMVLEFLGDSLLRLIR 133
>At4g34140.1 68417.m04845 D111/G-patch domain-containing protein
contains Pfam PF01585: G-patch domain
Length = 418
Score = 27.5 bits (58), Expect = 3.6
Identities = 11/34 (32%), Positives = 18/34 (52%)
Frame = -2
Query: 150 PSLEYTGPENQYGQALSRHEQERWVYPFWLVISS 49
P ++ E +G+A E+ERW+ + VI S
Sbjct: 134 PEEDFDPQEENFGEAAPSSEEERWLAQYGQVIES 167
>At1g29590.1 68414.m03618 eukaryotic translation initiation factor
4E, putative / eIF-4E, putative / eIF4E, putative / mRNA
cap-binding protein, putative similar to SP|O23252
Eukaryotic translation initiation factor 4E (eIF-4E)
(eIF4E) (mRNA cap-binding protein) (eIF-4F 25 kDa
subunit) (eIF-4F P26 subunit) {Arabidopsis thaliana};
contains Pfam profile PF01652: Eukaryotic initiation
factor 4E
Length = 285
Score = 27.1 bits (57), Expect = 4.8
Identities = 9/24 (37%), Positives = 14/24 (58%)
Frame = +2
Query: 95 CRESAWPYWFSGPVYSSEGRVHWG 166
C +++W +WF P S +V WG
Sbjct: 111 CFQNSWTFWFDNP-SSKSNQVIWG 133
>At1g29550.1 68414.m03614 eukaryotic translation initiation factor
4E, putative / eIF-4E, putative / eIF4E, putative / mRNA
cap-binding protein, putative similar to SP|O23252
Eukaryotic translation initiation factor 4E (eIF-4E)
(eIF4E) (mRNA cap-binding protein) (eIF-4F 25 kDa
subunit) (eIF-4F P26 subunit) {Arabidopsis thaliana};
contains Pfam profile PF01652: Eukaryotic initiation
factor 4E
Length = 240
Score = 27.1 bits (57), Expect = 4.8
Identities = 9/24 (37%), Positives = 14/24 (58%)
Frame = +2
Query: 95 CRESAWPYWFSGPVYSSEGRVHWG 166
C +++W +WF P S +V WG
Sbjct: 66 CFQNSWTFWFDNP-SSKSNQVIWG 88
>At1g29820.1 68414.m03645 expressed protein
Length = 540
Score = 26.6 bits (56), Expect = 6.3
Identities = 10/30 (33%), Positives = 16/30 (53%)
Frame = -2
Query: 105 LSRHEQERWVYPFWLVISSLMQLTHVHQSW 16
+ R+E WV W I+ L+Q+ V+ W
Sbjct: 108 VERYEGSHWVPIGWARITELVQMVQVNAEW 137
>At5g19460.1 68418.m02319 MutT/nudix family protein similar to
SP|P41888 Thiamine pyrophosphokinase (EC 2.7.6.2) (TPK)
(Thiamine kinase) {Schizosaccharomyces pombe}; contains
Pfam profile PF00293: NUDIX domain
Length = 374
Score = 26.2 bits (55), Expect = 8.3
Identities = 15/42 (35%), Positives = 24/42 (57%), Gaps = 2/42 (4%)
Frame = +3
Query: 3 FSKHASFGARV--LIALSSILQAKMDKPNVLARVVKVLGRTG 122
FS++ S RV + L+ +LQ D+ +A V+K+LG G
Sbjct: 128 FSQNGSCPDRVDGYVTLNLMLQKPEDRTRAVADVIKILGDKG 169
>At1g20260.2 68414.m02530 vacuolar ATP synthase subunit B, putative
/ V-ATPase B subunit, putative / vacuolar proton pump B
subunit, putative / V-ATPase 57 kDa subunit, putative
strong similarity to SP|P11574 Vacuolar ATP synthase
subunit B (EC 3.6.3.14) (V-ATPase B subunit) (Vacuolar
proton pump B subunit) (V-ATPase 57 kDa subunit)
{Arabidopsis thaliana}; contains Pfam profiles PF00006:
ATP synthase alpha/beta family nucleotide-binding
domain, PF02874: ATP synthase alpha/beta family
beta-barrel domain
Length = 485
Score = 26.2 bits (55), Expect = 8.3
Identities = 9/26 (34%), Positives = 13/26 (50%)
Frame = +2
Query: 83 RSCSCRESAWPYWFSGPVYSSEGRVH 160
R C R S+W W SG Y+ ++
Sbjct: 285 RFCCSRRSSWKTWISGVYYTDLATIY 310
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 8,471,105
Number of Sequences: 28952
Number of extensions: 152574
Number of successful extensions: 388
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 382
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 385
length of database: 12,070,560
effective HSP length: 74
effective length of database: 9,928,112
effective search space used: 595686720
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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