BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmnc1m07
(751 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC144.03 |ade2|min10, min3|adenylosuccinate synthetase Ade2|Sc... 27 3.8
SPCPJ732.02c |||xylulose kinase |Schizosaccharomyces pombe|chr 3... 27 3.8
SPAC3C7.13c |||glucose-6-phosphate 1-dehydrogenase |Schizosaccha... 26 5.0
SPBC365.08c |||Der1-like |Schizosaccharomyces pombe|chr 2|||Manual 26 6.6
SPBC17A3.04c |||methionine-tRNA ligase |Schizosaccharomyces pomb... 26 6.6
SPAC3F10.11c |abc2||glutathione S-conjugate-exporting ATPase Abc... 25 8.7
SPAC17A2.12 |||ATP-dependent DNA helicase|Schizosaccharomyces po... 25 8.7
>SPAC144.03 |ade2|min10, min3|adenylosuccinate synthetase
Ade2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 434
Score = 26.6 bits (56), Expect = 3.8
Identities = 17/43 (39%), Positives = 21/43 (48%), Gaps = 2/43 (4%)
Frame = -1
Query: 712 NSACISLRVKQSELHPPKCVQFLGGIVVVYL--F*SGVEKVLQ 590
N + S L PKC +G VVVYL F S +EK+ Q
Sbjct: 59 NGVTYDFHILPSGLVNPKCQNLIGSGVVVYLPAFFSELEKLEQ 101
>SPCPJ732.02c |||xylulose kinase |Schizosaccharomyces pombe|chr
3|||Manual
Length = 555
Score = 26.6 bits (56), Expect = 3.8
Identities = 18/62 (29%), Positives = 29/62 (46%)
Frame = -2
Query: 750 YSLCA*AC*WLP*TALVFRFGSSSPNYTRLSACNFWGASLLSIFFRVA*KKSCNCLLSKR 571
Y + A C WL L+F +S + +++ A + G S+F KK+ N L +K
Sbjct: 49 YEVFAPVCMWLDAIDLLFERLKASVDVSKIQAISGAGQQHASVFLLKGSKKALNSLDAKS 108
Query: 570 FL 565
L
Sbjct: 109 SL 110
>SPAC3C7.13c |||glucose-6-phosphate 1-dehydrogenase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 473
Score = 26.2 bits (55), Expect = 5.0
Identities = 12/34 (35%), Positives = 20/34 (58%), Gaps = 1/34 (2%)
Frame = +2
Query: 518 YDYSN-YIEFFSFVRSIKKRFDNRQLQDFFYATL 616
YD S+ Y+EF + S++K+ D+ + FY L
Sbjct: 83 YDQSSSYVEFRKHLESVEKKADSSKALRIFYIAL 116
>SPBC365.08c |||Der1-like |Schizosaccharomyces pombe|chr 2|||Manual
Length = 224
Score = 25.8 bits (54), Expect = 6.6
Identities = 10/18 (55%), Positives = 12/18 (66%)
Frame = +2
Query: 518 YDYSNYIEFFSFVRSIKK 571
Y YS Y+E F F R+ KK
Sbjct: 81 YQYSTYLENFVFARNAKK 98
>SPBC17A3.04c |||methionine-tRNA ligase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 782
Score = 25.8 bits (54), Expect = 6.6
Identities = 19/47 (40%), Positives = 26/47 (55%)
Frame = +2
Query: 92 YRSANAPAADINDTFLEKISIPRGHRDCCDAKVYETAVREFVEETGR 232
Y+ NAPAA I DT+ E +P GHR + + T + E +EE R
Sbjct: 730 YKQLNAPAAAIPDTW-ELCLLP-GHR-IGEPEYLFTRIDESMEEEWR 773
>SPAC3F10.11c |abc2||glutathione S-conjugate-exporting ATPase
Abc2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1463
Score = 25.4 bits (53), Expect = 8.7
Identities = 19/52 (36%), Positives = 26/52 (50%), Gaps = 2/52 (3%)
Frame = +2
Query: 554 VRSIKKRFDNRQL--QDFFYATLKKIDNNDAPQKLHALRRV*FGLLDPKRNT 703
+ SI KRF NRQ+ +D + +I NN KL+A + L RNT
Sbjct: 415 IASIFKRFQNRQMKNKDARSQFMTEIINNIRSIKLYAWENIFLQKLLQLRNT 466
>SPAC17A2.12 |||ATP-dependent DNA helicase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 897
Score = 25.4 bits (53), Expect = 8.7
Identities = 17/52 (32%), Positives = 25/52 (48%)
Frame = +2
Query: 545 FSFVRSIKKRFDNRQLQDFFYATLKKIDNNDAPQKLHALRRV*FGLLDPKRN 700
FS SIK R++NR Q F K+ + KL+ R+ ++ KRN
Sbjct: 690 FSEQESIKLRWENRIDQMFTKKFGKRASEWKSSSKLNQARQTILDIIGSKRN 741
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,968,347
Number of Sequences: 5004
Number of extensions: 60251
Number of successful extensions: 161
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 159
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 161
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 357280532
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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