BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmnc19m03
(608 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g05590.1 68416.m00621 60S ribosomal protein L18 (RPL18B) simi... 194 5e-50
At5g27850.1 68418.m03341 60S ribosomal protein L18 (RPL18C) 60S ... 187 4e-48
At2g47570.1 68415.m05936 60S ribosomal protein L18 (RPL18A) 153 7e-38
At1g18750.1 68414.m02338 MADS-box protein (AGL65) similar to hom... 31 0.45
At2g02640.1 68415.m00203 DC1 domain-containing protein contain... 31 0.60
At3g28360.1 68416.m03544 ABC transporter family protein similar ... 29 1.8
At2g04330.1 68415.m00429 hypothetical protein contains Pfam prof... 29 1.8
At5g35794.1 68418.m04297 hypothetical protein contains Pfam prof... 29 2.4
At4g03890.1 68417.m00546 hypothetical protein contains Pfam prof... 29 2.4
At2g23480.1 68415.m02803 hypothetical protein contains Pfam prof... 29 2.4
At3g28415.1 68416.m03551 P-glycoprotein, putative contains ATP-b... 28 4.2
At3g28390.1 68416.m03547 P-glycoprotein, putative similar to P-g... 28 4.2
At3g15470.1 68416.m01962 WD-40 repeat family protein contains Pf... 28 4.2
At1g05440.1 68414.m00552 expressed protein ; expression supporte... 28 4.2
At3g59820.1 68416.m06675 calcium-binding mitochondrial protein-r... 28 5.6
At5g67280.1 68418.m08483 leucine-rich repeat transmembrane prote... 27 7.4
At4g35730.1 68417.m05071 expressed protein contains Pfam profil... 27 7.4
At1g32190.1 68414.m03959 expressed protein 27 7.4
At3g28380.1 68416.m03546 P-glycoprotein, putative similar to P-g... 27 9.7
At3g28345.1 68416.m03541 ABC transporter family protein similar ... 27 9.7
>At3g05590.1 68416.m00621 60S ribosomal protein L18 (RPL18B) similar
to GB:P42791
Length = 187
Score = 194 bits (472), Expect = 5e-50
Identities = 99/184 (53%), Positives = 129/184 (70%), Gaps = 3/184 (1%)
Frame = +1
Query: 31 MGID-INHKHDRKVRRTEVKSQDIXXXXXXXXXXXXXXXTNAKFNQIVLRRLFMSRINRP 207
MGID I +K +RT KS D+ TN+KFN ++L+RLFMS++N+
Sbjct: 1 MGIDLIAGGKSKKTKRTAPKSDDVYLKLTVKLYRFLVRRTNSKFNGVILKRLFMSKVNKA 60
Query: 208 PISVSRLARHMKKPTREGLIAVVVGTVTNDVRLYKIPKMTVAALHVTEKARARILAAGGE 387
P+S+SRL M +E IAV+VGT+T+D+R+++IP M V AL TE+ARARI AGGE
Sbjct: 61 PLSLSRLVEFMTG--KEDKIAVLVGTITDDLRVHEIPAMKVTALRFTERARARIEKAGGE 118
Query: 388 ILTFDQLALRAPTGKKTVLVQGQRNAREAVRHFGPAPGAPRSHTKPYVRTKGH--EKARP 561
LTFDQLALRAP G+ TVL++G +N+REAV+HFGPAPG P SH+KPYVR KG EKAR
Sbjct: 119 CLTFDQLALRAPLGQNTVLLRGPKNSREAVKHFGPAPGVPHSHSKPYVRAKGRKFEKARG 178
Query: 562 SRRA 573
R++
Sbjct: 179 KRKS 182
>At5g27850.1 68418.m03341 60S ribosomal protein L18 (RPL18C) 60S
ribosomal protein L18, Arabidopsis thaliana,
SWISSPROT:RL18_ARATH
Length = 187
Score = 187 bits (456), Expect = 4e-48
Identities = 96/184 (52%), Positives = 128/184 (69%), Gaps = 3/184 (1%)
Frame = +1
Query: 31 MGID-INHKHDRKVRRTEVKSQDIXXXXXXXXXXXXXXXTNAKFNQIVLRRLFMSRINRP 207
MGID I +K +RT KS D+ +N+ FN ++L+RLFMS++N+
Sbjct: 1 MGIDLIAGGKSKKTKRTAPKSDDVYLKLLVKLYRFLVRRSNSNFNAVILKRLFMSKVNKA 60
Query: 208 PISVSRLARHMKKPTREGLIAVVVGTVTNDVRLYKIPKMTVAALHVTEKARARILAAGGE 387
P+S+SRL M ++ IAV+VGT+T+D+R+++IP M V AL TE+ARARI AGGE
Sbjct: 61 PLSLSRLVEFMTG--KDDKIAVLVGTITDDLRVHEIPAMKVTALRFTERARARIEKAGGE 118
Query: 388 ILTFDQLALRAPTGKKTVLVQGQRNAREAVRHFGPAPGAPRSHTKPYVRTKGH--EKARP 561
LTFDQLALRAP G+ TVL++G +N+REAV+HFGPAPG P S+TKPYVR KG EKAR
Sbjct: 119 CLTFDQLALRAPLGQNTVLLRGPKNSREAVKHFGPAPGVPHSNTKPYVRHKGRKFEKARG 178
Query: 562 SRRA 573
R++
Sbjct: 179 KRKS 182
>At2g47570.1 68415.m05936 60S ribosomal protein L18 (RPL18A)
Length = 135
Score = 153 bits (372), Expect = 7e-38
Identities = 79/132 (59%), Positives = 98/132 (74%), Gaps = 3/132 (2%)
Frame = +1
Query: 187 MSRINRPPISVSRLARHMKKPTREGLIAVVVGTVTNDVRLYKIPKMTVAALHVTEKARAR 366
MS++N+ P+S+SRL R+M ++G IAV+VGTVT+DVR+ +P +TV AL TE ARAR
Sbjct: 1 MSKVNKAPLSLSRLVRYMDG--KDGKIAVIVGTVTDDVRIEDVPALTVTALRFTESARAR 58
Query: 367 ILAAGGEILTFDQLALRAPT-GKKTVLVQGQRNAREAVRHFGPAPGAPRSHTKPYVRTKG 543
I AGGE LTFDQLAL PT + TVL++G +N REAV+HFGPAPG P SHTKPYVR G
Sbjct: 59 IHKAGGECLTFDQLALPCPTWSENTVLLRGPKNTREAVKHFGPAPGVPHSHTKPYVRQTG 118
Query: 544 H--EKARPSRRA 573
E AR RR+
Sbjct: 119 KKIEIARGRRRS 130
>At1g18750.1 68414.m02338 MADS-box protein (AGL65) similar to
homeodomain transcription factor (AGL30) GI:3461830 from
[Arabidopsis thaliana]; contains Pfam domain PF00319:
SRF-type transcription factor (DNA-binding and
dimerisation domain); PMID: 12837945
Length = 389
Score = 31.5 bits (68), Expect = 0.45
Identities = 14/31 (45%), Positives = 16/31 (51%)
Frame = -2
Query: 490 GQSDALPHEHFADLVPVLSSCQSEHEEPADQ 398
G S LPH +PV SSC E +P DQ
Sbjct: 223 GDSSFLPHREMDGSIPVYSSCFFESTKPEDQ 253
>At2g02640.1 68415.m00203 DC1 domain-containing protein contains
Pfam profile PF03107: DC1 domain
Length = 627
Score = 31.1 bits (67), Expect = 0.60
Identities = 11/22 (50%), Positives = 15/22 (68%)
Frame = +3
Query: 333 CSSCYRKSSCTHFGCWRRNSYF 398
CS+C RKS+ + C+ RN YF
Sbjct: 423 CSACMRKSNGFGYSCYNRNCYF 444
>At3g28360.1 68416.m03544 ABC transporter family protein similar to
P-glycoprotein homologue GI:2292907 from [Hordeum
vulgare subsp. vulgare]
Length = 1158
Score = 29.5 bits (63), Expect = 1.8
Identities = 13/31 (41%), Positives = 17/31 (54%)
Frame = +1
Query: 385 EILTFDQLALRAPTGKKTVLVQGQRNAREAV 477
E L FD L L+ P+GK LV G + + V
Sbjct: 290 ETLIFDDLCLKIPSGKTVALVGGSGSGKSTV 320
>At2g04330.1 68415.m00429 hypothetical protein contains Pfam profile
PF03384: Drosophila protein of unknown function, DUF287
Length = 564
Score = 29.5 bits (63), Expect = 1.8
Identities = 16/46 (34%), Positives = 22/46 (47%)
Frame = -1
Query: 314 ILYSLTSFVTVPTTTAIKPSRVGFFMWRAKRDTEIGGRLIRLIKSR 177
+LY LTS + VPT T + S + F RA D I ++ R
Sbjct: 347 VLYFLTSSIVVPTKTGERASPIDDFCVRAASDLTFSFEAIPSLRER 392
>At5g35794.1 68418.m04297 hypothetical protein contains Pfam profile
PF03384: Drosophila protein of unknown function, DUF287
Length = 662
Score = 29.1 bits (62), Expect = 2.4
Identities = 14/32 (43%), Positives = 18/32 (56%)
Frame = -1
Query: 314 ILYSLTSFVTVPTTTAIKPSRVGFFMWRAKRD 219
+LY LTS + VPT T + S + F RA D
Sbjct: 377 VLYFLTSIIAVPTKTGERASPIDDFCVRAASD 408
>At4g03890.1 68417.m00546 hypothetical protein contains Pfam profile
PF03384: Drosophila protein of unknown function, DUF287
Length = 301
Score = 29.1 bits (62), Expect = 2.4
Identities = 16/33 (48%), Positives = 18/33 (54%)
Frame = -1
Query: 317 GILYSLTSFVTVPTTTAIKPSRVGFFMWRAKRD 219
G+LY LTS + V T T K S V F RA D
Sbjct: 101 GVLYFLTSIIVVLTKTGEKGSPVDNFCLRAASD 133
>At2g23480.1 68415.m02803 hypothetical protein contains Pfam profile
PF03384: Drosophila protein of unknown function, DUF287
Length = 705
Score = 29.1 bits (62), Expect = 2.4
Identities = 14/32 (43%), Positives = 18/32 (56%)
Frame = -1
Query: 314 ILYSLTSFVTVPTTTAIKPSRVGFFMWRAKRD 219
+LY LTS + VPT T + S + F RA D
Sbjct: 440 VLYFLTSIIAVPTKTGERASPIDDFCVRAASD 471
>At3g28415.1 68416.m03551 P-glycoprotein, putative contains
ATP-binding cassette; related to multi drug resistance
proteins
Length = 1221
Score = 28.3 bits (60), Expect = 4.2
Identities = 12/27 (44%), Positives = 15/27 (55%)
Frame = +1
Query: 397 FDQLALRAPTGKKTVLVQGQRNAREAV 477
FD L LR P+GK LV G + + V
Sbjct: 356 FDDLCLRIPSGKSVALVGGSGSGKSTV 382
>At3g28390.1 68416.m03547 P-glycoprotein, putative similar to
P-glycoprotein homologue GI:2292907 from [Hordeum
vulgare subsp. vulgare]
Length = 1225
Score = 28.3 bits (60), Expect = 4.2
Identities = 12/27 (44%), Positives = 15/27 (55%)
Frame = +1
Query: 397 FDQLALRAPTGKKTVLVQGQRNAREAV 477
FD L LR P+GK LV G + + V
Sbjct: 365 FDDLCLRVPSGKTVALVGGSGSGKSTV 391
>At3g15470.1 68416.m01962 WD-40 repeat family protein contains Pfam
PF00400: WD domain, G-beta repeat; similar to WD-repeat
protein 5 (WD repeat protein BIG-3) (SP: Q9UGP9) [Homo
sapiens]
Length = 883
Score = 28.3 bits (60), Expect = 4.2
Identities = 16/44 (36%), Positives = 23/44 (52%), Gaps = 2/44 (4%)
Frame = +3
Query: 297 RETVQDTEDDGGCSSCY--RKSSCTHFGCWRRNSYF*SAGSSCS 422
R+ + +E GGC+SC KS T C R+ + S G+ CS
Sbjct: 145 RDHGECSESVGGCASCIVRSKSDITTSQCGDRDRRYTSPGNPCS 188
>At1g05440.1 68414.m00552 expressed protein ; expression supported
by MPSS
Length = 393
Score = 28.3 bits (60), Expect = 4.2
Identities = 14/41 (34%), Positives = 24/41 (58%)
Frame = -3
Query: 474 CLTSISLTLYQYCLLASRSTKSQLIKSKNFSSSSQNACTSF 352
CL S++ TL L +S+ ++L + NF S+ ++ TSF
Sbjct: 71 CLDSLNATLDLMPLSVQKSSLTKLSSASNFKSTVESTPTSF 111
>At3g59820.1 68416.m06675 calcium-binding mitochondrial
protein-related contains weak similarity to
Calcium-binding mitochondrial protein Anon-60Da
(Swiss-Prot:P91927) [Drosophila melanogaster]
Length = 755
Score = 27.9 bits (59), Expect = 5.6
Identities = 12/36 (33%), Positives = 18/36 (50%)
Frame = +1
Query: 160 NQIVLRRLFMSRINRPPISVSRLARHMKKPTREGLI 267
+Q V + S INRPP+ + H R+GL+
Sbjct: 34 SQTVHSHAYHSGINRPPVETKPVTEHKSFTRRDGLL 69
>At5g67280.1 68418.m08483 leucine-rich repeat transmembrane protein
kinase, putative
Length = 751
Score = 27.5 bits (58), Expect = 7.4
Identities = 20/61 (32%), Positives = 30/61 (49%), Gaps = 2/61 (3%)
Frame = -2
Query: 385 LLQQPKCVHELFR*HEEQPPS-SSVSCTVS-RHL*LSPLPRQSNPHELASSCGAPNETQR 212
++ P V +R +E P S V+C S RH+ + LP + L S+ G+ N QR
Sbjct: 45 IVDDPLYVFRSWRFDDETPCSWRGVTCDASSRHVTVLSLPSSNLTGTLPSNLGSLNSLQR 104
Query: 211 L 209
L
Sbjct: 105 L 105
>At4g35730.1 68417.m05071 expressed protein contains Pfam profile:
PF03398 eukaryotic protein of unknown function, DUF292
Length = 430
Score = 27.5 bits (58), Expect = 7.4
Identities = 16/44 (36%), Positives = 24/44 (54%)
Frame = +3
Query: 324 DGGCSSCYRKSSCTHFGCWRRNSYF*SAGSSCSDWQEDSTGTRS 455
D SS Y K + G RR+SY + G + SD++E+ T T +
Sbjct: 289 DSETSSYYAKPGAENRGMGRRHSYN-NPGINESDYEEEYTNTEA 331
>At1g32190.1 68414.m03959 expressed protein
Length = 422
Score = 27.5 bits (58), Expect = 7.4
Identities = 8/17 (47%), Positives = 10/17 (58%)
Frame = +3
Query: 333 CSSCYRKSSCTHFGCWR 383
CSSC+ K C CW+
Sbjct: 359 CSSCFGKPKCPKCSCWK 375
>At3g28380.1 68416.m03546 P-glycoprotein, putative similar to
P-glycoprotein homologue GI:2292907 from [Hordeum
vulgare subsp. vulgare]
Length = 1240
Score = 27.1 bits (57), Expect = 9.7
Identities = 12/31 (38%), Positives = 15/31 (48%)
Frame = +1
Query: 385 EILTFDQLALRAPTGKKTVLVQGQRNAREAV 477
E FD L L+ P GK LV G + + V
Sbjct: 373 ETTIFDDLCLKIPAGKTVALVGGSGSGKSTV 403
>At3g28345.1 68416.m03541 ABC transporter family protein similar to
P-glycoprotein [Arabidopsis thaliana] GI:3849833;
contains Pfam profiles PF00005: ABC transporter,
PF00664: ABC transporter transmembrane region
Length = 1240
Score = 27.1 bits (57), Expect = 9.7
Identities = 12/31 (38%), Positives = 15/31 (48%)
Frame = +1
Query: 385 EILTFDQLALRAPTGKKTVLVQGQRNAREAV 477
E FD LR P+GK LV G + + V
Sbjct: 373 ETSIFDDFCLRVPSGKTVALVGGSGSGKSTV 403
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,135,409
Number of Sequences: 28952
Number of extensions: 310562
Number of successful extensions: 857
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 824
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 852
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1216725696
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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