Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= bmnc19g08
         (481 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC576.07 |ret3||coatomer zeta subunit |Schizosaccharomyces pom...    83   3e-17
SPBC1709.03 |||conserved fungal protein|Schizosaccharomyces pomb...    26   3.4  
SPCC1827.01c |||DUF1253 family protein|Schizosaccharomyces pombe...    25   4.5  
SPCC74.01 |sly1||SNARE binding protein Sly1|Schizosaccharomyces ...    25   5.9  
SPBC1703.14c |top1||DNA topoisomerase I|Schizosaccharomyces pomb...    25   5.9  
SPAC12B10.01c ||SPAC31F12.02c, SPAC637.15c|ubiquitin-protein lig...    25   7.8  
SPAC23H3.11c |||glucosidase |Schizosaccharomyces pombe|chr 1|||M...    25   7.8  
SPCC11E10.05c |ynd1||nucleoside diphosphatase |Schizosaccharomyc...    25   7.8  

>SPCC576.07 |ret3||coatomer zeta subunit |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 190

 Score = 82.6 bits (195), Expect = 3e-17
 Identities = 43/124 (34%), Positives = 68/124 (54%), Gaps = 8/124 (6%)
 Frame = +1

Query: 127 TLYIVKGMCILDYEGNRILAXYY--------DKDVLPTTKEQKAFEKNLFNKTHRANAEI 282
           TLY V    ILD  G RI   YY        +  V  + KE+K FEK LF KT +   +I
Sbjct: 4   TLYAVNAFLILDSSGKRIFTKYYAPPHLKEGEGGVFNSVKEEKTFEKGLFEKTWKTQNDI 63

Query: 283 IMLDGLTCVYKSNVDLFFYVMGSSHENELILQSVLNALYESVSLLLRRNMERRVLMENLD 462
           +  DG   V  + +D+ FY++G   ENE++L   L ++ +++ LL +   ++R L+EN D
Sbjct: 64  LTYDGKLVVMLTVMDVIFYIVGGMEENEVMLYECLRSIRDALELLFKYVPDKRTLLENYD 123

Query: 463 AVML 474
            +++
Sbjct: 124 QLVI 127


>SPBC1709.03 |||conserved fungal protein|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 398

 Score = 25.8 bits (54), Expect = 3.4
 Identities = 9/16 (56%), Positives = 10/16 (62%)
 Frame = +3

Query: 429 HGAESPYGELGCCYAC 476
           HG  S YG+L  CY C
Sbjct: 306 HGRCSKYGQLDSCYVC 321


>SPCC1827.01c |||DUF1253 family protein|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 652

 Score = 25.4 bits (53), Expect = 4.5
 Identities = 20/78 (25%), Positives = 34/78 (43%), Gaps = 13/78 (16%)
 Frame = +1

Query: 226 QKAFEKNLFNKTHRANAEIIMLDGLTCVYKS-------------NVDLFFYVMGSSHENE 366
           Q  F  N+  K+H+    ++ L  L  V+K+             N +L F   G +    
Sbjct: 201 QDVFFTNMSMKSHKLGTSLLALHALNHVFKTRDRVLKNSARISQNPELEFRDQGYTRPKV 260

Query: 367 LILQSVLNALYESVSLLL 420
           LIL    N+ +E ++LL+
Sbjct: 261 LILLPTRNSAFEFINLLI 278


>SPCC74.01 |sly1||SNARE binding protein Sly1|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 639

 Score = 25.0 bits (52), Expect = 5.9
 Identities = 11/44 (25%), Positives = 22/44 (50%)
 Frame = +1

Query: 316 SNVDLFFYVMGSSHENELILQSVLNALYESVSLLLRRNMERRVL 447
           ++V   ++V  +    ELI++ +   LYES  +     + R +L
Sbjct: 87  ADVPAIYFVQPTQENIELIIEDLSKGLYESAYVCFSSTISRALL 130


>SPBC1703.14c |top1||DNA topoisomerase I|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 814

 Score = 25.0 bits (52), Expect = 5.9
 Identities = 10/35 (28%), Positives = 21/35 (60%)
 Frame = +1

Query: 196 DKDVLPTTKEQKAFEKNLFNKTHRANAEIIMLDGL 300
           D+D +P  K + + +KN+ N + +  A+++   GL
Sbjct: 55  DEDDIPIRKRRASSKKNMSNSSSKKRAKVMGNGGL 89


>SPAC12B10.01c ||SPAC31F12.02c, SPAC637.15c|ubiquitin-protein ligase
            E3 |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1647

 Score = 24.6 bits (51), Expect = 7.8
 Identities = 17/38 (44%), Positives = 21/38 (55%)
 Frame = +1

Query: 367  LILQSVLNALYESVSLLLRRNMERRVLMENLDAVMLAF 480
            L+LQS    L  SVS LL RN E   L +N  + +L F
Sbjct: 1236 LLLQSTAFGLSRSVSFLLSRNPE---LSKNESSSILQF 1270


>SPAC23H3.11c |||glucosidase |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 629

 Score = 24.6 bits (51), Expect = 7.8
 Identities = 10/29 (34%), Positives = 18/29 (62%)
 Frame = -3

Query: 119 NREPSIVV*TIVWVNNFSLYYYFKELKIP 33
           + EP  ++  +   NN++ YYYF++L  P
Sbjct: 535 SEEPMSIIFNLGISNNWA-YYYFRDLSFP 562


>SPCC11E10.05c |ynd1||nucleoside diphosphatase |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 572

 Score = 24.6 bits (51), Expect = 7.8
 Identities = 19/58 (32%), Positives = 28/58 (48%), Gaps = 1/58 (1%)
 Frame = +1

Query: 103 MEGSLFEPTLYI-VKGMCILDYEGNRILAXYYDKDVLPTTKEQKAFEKNLFNKTHRAN 273
           M GS   P  Y  V   C  ++E   +L+  Y+K++ P+T E K  EK  F  +   N
Sbjct: 325 MGGSYHFPNFYKKVDEYCGTEWE--TMLSRLYNKELTPSTDENK-LEKLCFKASWALN 379


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,049,392
Number of Sequences: 5004
Number of extensions: 41406
Number of successful extensions: 84
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 80
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 83
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 184020746
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)