Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= bmnc19f04
         (627 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC19C2.09 |sre1||sterol regulatory element binding protein Sre...    31   0.10 
SPBC215.05 |gpd1||glycerol-3-phosphate dehydrogenase Gpd1|Schizo...    29   0.55 
SPAC11E3.08c |nse6||Smc5-6 complex non-SMC subunit Nse6|Schizosa...    27   2.2  
SPAC6F6.06c |rax2||cell polarity factor Rax2|Schizosaccharomyces...    27   2.2  
SPAC2G11.02 |urb2||ribosome biogenesis protein Urb2 |Schizosacch...    27   2.2  
SPAC589.02c |med13|spTrap240, srb9|mediator complex subunit Srb9...    27   2.9  
SPAC30C2.07 |||sequence orphan|Schizosaccharomyces pombe|chr 1||...    26   3.9  
SPBC4.03c |||COPII-coated vesicle component Sfb3 |Schizosaccharo...    26   5.1  
SPBC19G7.10c |||topoisomerase associated protein |Schizosaccharo...    26   5.1  
SPAC56F8.10 |met9|met5|methylenetetrahydrofolate reductase Met9|...    25   6.8  
SPAC19A8.04 |erg5||C-22 sterol desaturase Erg5 |Schizosaccharomy...    25   6.8  
SPAC821.13c ||SPAC955.01c|P-type ATPase |Schizosaccharomyces pom...    25   6.8  
SPCC162.06c |||vacuolar sorting protein Vps60|Schizosaccharomyce...    25   6.8  
SPAC227.11c |||sensor for misfolded ER glycoproteins Yos9 |Schiz...    25   6.8  
SPAC1A6.04c |plb1||phospholipase B homolog Plb1|Schizosaccharomy...    25   9.0  
SPCC1322.12c |bub1||serine/threonine protein kinase Bub1|Schizos...    25   9.0  
SPBC19F5.05c |ppp1|SPBC25D12.01c|pescadillo-family BRCT domain p...    25   9.0  

>SPBC19C2.09 |sre1||sterol regulatory element binding protein
           Sre1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 900

 Score = 31.5 bits (68), Expect = 0.10
 Identities = 23/84 (27%), Positives = 39/84 (46%)
 Frame = -3

Query: 553 LTP*SYTVPRKMLLVPTLLEPSLTMSSIDSGFSAIYIWLNLLSPCAMRKLATRALLSFTF 374
           L P + ++ R  LL   +L P  T  S++   +A   + N L  C+  K    ++L    
Sbjct: 731 LCPKNSSIIRHALLAKLVLFPENTADSLNEVLAA---YKNTLDLCSQDKRKQSSVLKINL 787

Query: 373 NRLFSSDSTDSLLCPKLGDASVKE 302
           ++LF+  S  SL   +LG   V +
Sbjct: 788 SKLFTLHSCLSLALQRLGYGDVSK 811


>SPBC215.05 |gpd1||glycerol-3-phosphate dehydrogenase
           Gpd1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 385

 Score = 29.1 bits (62), Expect = 0.55
 Identities = 14/40 (35%), Positives = 20/40 (50%), Gaps = 2/40 (5%)
 Frame = -2

Query: 614 KVCG--AARHGHITRCAGRVWRFNSIVVYGAQKNAVSAHF 501
           K+CG  A  HGH  R   R+W F   + Y  +K  ++  F
Sbjct: 39  KICGENARAHGHHFRSKVRMWVFEEEIEYKGEKRKLTEVF 78


>SPAC11E3.08c |nse6||Smc5-6 complex non-SMC subunit
           Nse6|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 522

 Score = 27.1 bits (57), Expect = 2.2
 Identities = 18/70 (25%), Positives = 32/70 (45%), Gaps = 3/70 (4%)
 Frame = -3

Query: 511 VPTLLEPSLTMSSIDSGFSAIYIWLNLLSPCAMRKLATRALLSFTF---NRLFSSDSTDS 341
           +P  L P   +S+I     +  +W+ +LS  +      R  ++FT     +  + DS  S
Sbjct: 318 LPISLNPDFLISNIHKVSESPRVWVTILSSLSRSCQKFRKKIAFTLFVGKQSKNDDSDFS 377

Query: 340 LLCPKLGDAS 311
            LC +L + S
Sbjct: 378 SLCQRLDEIS 387


>SPAC6F6.06c |rax2||cell polarity factor Rax2|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1155

 Score = 27.1 bits (57), Expect = 2.2
 Identities = 13/51 (25%), Positives = 22/51 (43%)
 Frame = +1

Query: 37  SAFGSNVINYCEQFITAIFEIFTLSSKIVVAVPVNWENDNLSVLLKHLHNL 189
           S FG+    +C+      +  FT S K        W   N +V ++H +N+
Sbjct: 120 SFFGNISAIFCDDKFPYAYATFTFSDKPSFTSIYRWNVTNSNVTIEHFYNV 170


>SPAC2G11.02 |urb2||ribosome biogenesis protein Urb2
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1318

 Score = 27.1 bits (57), Expect = 2.2
 Identities = 15/39 (38%), Positives = 20/39 (51%)
 Frame = +2

Query: 8   LRHM*IWSTSLRSAATLLTIANSLSRPFSKFLRSAVKSL 124
           LR    WSTS    ++LL I   +  P SK L   +KS+
Sbjct: 353 LRQNTCWSTSWEMLSSLLAIDFDVLLPHSKILWECIKSV 391


>SPAC589.02c |med13|spTrap240, srb9|mediator complex subunit
            Srb9|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1223

 Score = 26.6 bits (56), Expect = 2.9
 Identities = 14/42 (33%), Positives = 23/42 (54%)
 Frame = +3

Query: 474  DDIVKEGSNKVGTNSIFLGTVYDYGVKSPNAASTSSNVTMTR 599
            D I + G NK    S+ + T+ +    SPN+ +T+SN   T+
Sbjct: 891  DSIKRLGDNKFEDKSLRICTIPNSIFDSPNSHTTNSNSFFTK 932


>SPAC30C2.07 |||sequence orphan|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 842

 Score = 26.2 bits (55), Expect = 3.9
 Identities = 12/39 (30%), Positives = 18/39 (46%)
 Frame = +1

Query: 73  QFITAIFEIFTLSSKIVVAVPVNWENDNLSVLLKHLHNL 189
           Q + A  + ++L    +   P  WEND    LLK  H +
Sbjct: 750 QLMVANLDTYSLDCYEIHEFPSEWENDYAPFLLKEHHKV 788


>SPBC4.03c |||COPII-coated vesicle component Sfb3
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 891

 Score = 25.8 bits (54), Expect = 5.1
 Identities = 12/31 (38%), Positives = 19/31 (61%)
 Frame = -3

Query: 553 LTP*SYTVPRKMLLVPTLLEPSLTMSSIDSG 461
           LTP    +P+ ++L+P L+   L  S+I SG
Sbjct: 673 LTPGQLVLPKNLVLLPILILAVLKSSAIRSG 703


>SPBC19G7.10c |||topoisomerase associated protein
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 744

 Score = 25.8 bits (54), Expect = 5.1
 Identities = 18/53 (33%), Positives = 27/53 (50%), Gaps = 2/53 (3%)
 Frame = -3

Query: 607 AVPRVMVTLLDVLAAFGDLTP*SYTVPRKMLLVPTLLEPSLTMSSID--SGFS 455
           A  R   T L +  A G +   +   PR++L V    EP+ + SS++  SGFS
Sbjct: 399 AKERPKATQLSLEGALGKIAVNTVRTPRQLLNVKRPTEPASSNSSLNNFSGFS 451


>SPAC56F8.10 |met9|met5|methylenetetrahydrofolate reductase
           Met9|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 603

 Score = 25.4 bits (53), Expect = 6.8
 Identities = 13/43 (30%), Positives = 24/43 (55%), Gaps = 1/43 (2%)
 Frame = -3

Query: 529 PRKMLLVPTLLEPSLTMSSIDSGFSAIYIWLNLLSPCAM-RKL 404
           P + ++ PT++E +  ++  D  +S    W N  SP ++ RKL
Sbjct: 514 PNREIIQPTIVESTSFLAWKDEAYSLGMEWANAYSPDSISRKL 556


>SPAC19A8.04 |erg5||C-22 sterol desaturase Erg5 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 541

 Score = 25.4 bits (53), Expect = 6.8
 Identities = 14/34 (41%), Positives = 21/34 (61%)
 Frame = -3

Query: 601 PRVMVTLLDVLAAFGDLTP*SYTVPRKMLLVPTL 500
           P V++    V  AF  +TP  YTVP+  +++PTL
Sbjct: 384 PPVLMVPYRVKKAF-PITP-DYTVPKDAMVIPTL 415


>SPAC821.13c ||SPAC955.01c|P-type ATPase |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1562

 Score = 25.4 bits (53), Expect = 6.8
 Identities = 17/58 (29%), Positives = 30/58 (51%)
 Frame = +3

Query: 420 HGDNKLSQMYIAEKPLSIDDIVKEGSNKVGTNSIFLGTVYDYGVKSPNAASTSSNVTM 593
           +G  KL  +   E PLS +D++  GSN   T+ +F   ++  G +S    +   NV++
Sbjct: 508 NGSMKLDSIQ-GEIPLSNNDVLYRGSNLRNTSELFALVIFT-GEESKIRMNAVRNVSV 563


>SPCC162.06c |||vacuolar sorting protein Vps60|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 210

 Score = 25.4 bits (53), Expect = 6.8
 Identities = 18/49 (36%), Positives = 28/49 (57%), Gaps = 2/49 (4%)
 Frame = +3

Query: 303 SLTEASPSLGQSSESVESDENKRLNVKLN--NARVANLRIAHGDNKLSQ 443
           SLT+A  SL + S+SVE  +  +L+ +L+    ++AN R   G   L Q
Sbjct: 16  SLTDAIDSLDKRSDSVEV-KIAKLDAQLSVFQQKIANTRPGPGQTALKQ 63


>SPAC227.11c |||sensor for misfolded ER glycoproteins Yos9
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 322

 Score = 25.4 bits (53), Expect = 6.8
 Identities = 12/38 (31%), Positives = 19/38 (50%)
 Frame = +3

Query: 477 DIVKEGSNKVGTNSIFLGTVYDYGVKSPNAASTSSNVT 590
           D +KE + K   +S+F    ++Y    PNA+    N T
Sbjct: 64  DSIKEKTEKTKLSSLFYAGKHEYFCVYPNASLIKQNST 101


>SPAC1A6.04c |plb1||phospholipase B homolog Plb1|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 613

 Score = 25.0 bits (52), Expect = 9.0
 Identities = 13/55 (23%), Positives = 25/55 (45%)
 Frame = -2

Query: 566 RVWRFNSIVVYGAQKNAVSAHFVGALFDYVVYR*RFFGNIHLAQFIIAVCDAQIG 402
           ++W FN  ++Y       +AHF  ++ + V  +     NI L+ +   V    +G
Sbjct: 182 KLWAFNHPLMYPKSAIVFNAHFYSSIMNEVAEKANAGFNISLSDYWGRVISRTLG 236


>SPCC1322.12c |bub1||serine/threonine protein kinase
           Bub1|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 1044

 Score = 25.0 bits (52), Expect = 9.0
 Identities = 12/43 (27%), Positives = 20/43 (46%)
 Frame = +3

Query: 450 IAEKPLSIDDIVKEGSNKVGTNSIFLGTVYDYGVKSPNAASTS 578
           I  +PL  + + K   + +   S +LGT       S N+ +TS
Sbjct: 396 IFNQPLRSESLEKSSKSPISAQSSYLGTPLKNDENSSNSGATS 438


>SPBC19F5.05c |ppp1|SPBC25D12.01c|pescadillo-family BRCT domain
           protein|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 607

 Score = 25.0 bits (52), Expect = 9.0
 Identities = 13/31 (41%), Positives = 17/31 (54%)
 Frame = +3

Query: 414 IAHGDNKLSQMYIAEKPLSIDDIVKEGSNKV 506
           I HG+NK ++  IA     I DIV   +N V
Sbjct: 274 IVHGNNKNARKNIATLKSKIRDIVNSDANVV 304


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,517,455
Number of Sequences: 5004
Number of extensions: 49203
Number of successful extensions: 162
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 157
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 162
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 277683324
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)