BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmnc19e05
(635 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g09630.1 68416.m01142 60S ribosomal protein L4/L1 (RPL4A) str... 208 3e-54
At5g02870.1 68418.m00230 60S ribosomal protein L4/L1 (RPL4D) 60S... 206 1e-53
At2g43680.2 68415.m05430 calmodulin-binding family protein simil... 30 1.1
At2g43680.1 68415.m05429 calmodulin-binding family protein simil... 30 1.1
At1g57750.1 68414.m06552 cytochrome P450, putative similar to cy... 29 2.0
At5g55100.2 68418.m06869 SWAP (Suppressor-of-White-APricot)/surp... 29 2.6
At5g55100.1 68418.m06868 SWAP (Suppressor-of-White-APricot)/surp... 29 2.6
At4g39480.1 68417.m05585 cytochrome P450 family protein contains... 28 4.5
At1g26400.1 68414.m03220 FAD-binding domain-containing protein s... 28 4.5
At5g17650.1 68418.m02069 glycine/proline-rich protein glycine/pr... 28 6.0
At5g01010.1 68418.m00001 expressed protein 28 6.0
At3g12430.1 68416.m01548 expressed protein ; expression supporte... 28 6.0
At5g07520.1 68418.m00861 glycine-rich protein (GRP18) Oleosin; g... 27 7.9
At1g80480.1 68414.m09427 PRLI-interacting factor L, putative sim... 27 7.9
>At3g09630.1 68416.m01142 60S ribosomal protein L4/L1 (RPL4A) strong
similarity to 60S ribosomal protein L1 GB:P49691
Length = 406
Score = 208 bits (507), Expect = 3e-54
Identities = 99/184 (53%), Positives = 122/184 (66%), Gaps = 2/184 (1%)
Frame = +3
Query: 87 MSLSVARPLVSVYSEKSE--TVQGAAKPLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCVS 260
M+ + ARPLV++ + + T Q + LP V AP+RPD+VN VH +S NSRQPY VS
Sbjct: 1 MAAAAARPLVTIQTLDGDMSTDQSSTVVLPDVMTAPVRPDIVNFVHAQISNNSRQPYAVS 60
Query: 261 KEAGHQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPTKPWRRWHXXX 440
K+AGHQTSAESWGTGRAV+RIPRV GGGTHR+GQ AFGNMCRGGRMFAPTK WRRWH
Sbjct: 61 KKAGHQTSAESWGTGRAVSRIPRVPGGGTHRAGQAAFGNMCRGGRMFAPTKIWRRWHRRV 120
Query: 441 XXXXXXXXXXXXXXXXXXXXXXQARGHIIEKIPELPLVVADKVQEINKTKQAVIFLRRLK 620
ARGH IE +PE+PLVV+D + + KT A+ L+++
Sbjct: 121 NVNMKRHAIVSAIAATAVPALVMARGHKIENVPEMPLVVSDSAEAVEKTSAAIKVLKQIG 180
Query: 621 AWSD 632
A+ D
Sbjct: 181 AYDD 184
>At5g02870.1 68418.m00230 60S ribosomal protein L4/L1 (RPL4D) 60S
roibosomal protein L4, Arabidopsis thaliana,
EMBL:CAA79104
Length = 407
Score = 206 bits (502), Expect = 1e-53
Identities = 99/181 (54%), Positives = 118/181 (65%), Gaps = 2/181 (1%)
Frame = +3
Query: 96 SVARPLVSVYSEKSE--TVQGAAKPLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCVSKEA 269
+ ARPLV+V + T Q LP V AP+RPD+VN VH +S NSRQPY VSK+A
Sbjct: 5 AAARPLVTVQGLDGDMSTDQSTTVTLPDVMTAPVRPDIVNFVHAQISNNSRQPYAVSKKA 64
Query: 270 GHQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPTKPWRRWHXXXXXX 449
GHQTSAESWGTGRAV+RIPRV GGGTHR+GQ AFGNMCRGGRMFAPTK WRRWH
Sbjct: 65 GHQTSAESWGTGRAVSRIPRVPGGGTHRAGQAAFGNMCRGGRMFAPTKIWRRWHRRVNVN 124
Query: 450 XXXXXXXXXXXXXXXXXXXQARGHIIEKIPELPLVVADKVQEINKTKQAVIFLRRLKAWS 629
ARGH IE +PE+PLVV+D + + KT A+ L+++ A+
Sbjct: 125 MKRHAIVSAIAATAVPALVMARGHKIENVPEMPLVVSDSAEAVEKTSAAIKVLKQIGAYD 184
Query: 630 D 632
D
Sbjct: 185 D 185
>At2g43680.2 68415.m05430 calmodulin-binding family protein similar
to SF16 protein [Helianthus annuus] GI:560150; contains
Pfam profile PF00612: IQ calmodulin-binding motif
Length = 669
Score = 30.3 bits (65), Expect = 1.1
Identities = 19/61 (31%), Positives = 26/61 (42%)
Frame = +2
Query: 386 WWTYVRPHEALAALAPSRQPPTAESGLGGSRCCYRRPSARSG*RTHY*KDSRASLGCSRQ 565
WW +V LA+ APS P + L SR C P ++S + H D+ R
Sbjct: 465 WWNWVDRQNPLASPAPSYSQPQRDFRLTPSRLC-PSPLSQSSKQHHIRLDNHFDTSTPRS 523
Query: 566 S 568
S
Sbjct: 524 S 524
>At2g43680.1 68415.m05429 calmodulin-binding family protein similar
to SF16 protein [Helianthus annuus] GI:560150; contains
Pfam profile PF00612: IQ calmodulin-binding motif
Length = 668
Score = 30.3 bits (65), Expect = 1.1
Identities = 19/61 (31%), Positives = 26/61 (42%)
Frame = +2
Query: 386 WWTYVRPHEALAALAPSRQPPTAESGLGGSRCCYRRPSARSG*RTHY*KDSRASLGCSRQ 565
WW +V LA+ APS P + L SR C P ++S + H D+ R
Sbjct: 464 WWNWVDRQNPLASPAPSYSQPQRDFRLTPSRLC-PSPLSQSSKQHHIRLDNHFDTSTPRS 522
Query: 566 S 568
S
Sbjct: 523 S 523
>At1g57750.1 68414.m06552 cytochrome P450, putative similar to
cytochrome P450 GI:4688670 from [Catharanthus roseus]
Length = 497
Score = 29.5 bits (63), Expect = 2.0
Identities = 16/49 (32%), Positives = 29/49 (59%)
Frame = +3
Query: 111 LVSVYSEKSETVQGAAKPLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCV 257
LV +++ SE+++ PLPF K+P +PD++ H + NS+ C+
Sbjct: 351 LVYLHAALSESMR-LYPPLPFNHKSPAKPDVLPSGH-KVDANSKIVICI 397
>At5g55100.2 68418.m06869 SWAP (Suppressor-of-White-APricot)/surp
domain-containing protein contains Pfam domain PF01805:
Surp module
Length = 844
Score = 29.1 bits (62), Expect = 2.6
Identities = 12/41 (29%), Positives = 21/41 (51%), Gaps = 1/41 (2%)
Frame = -3
Query: 402 RTYVHHDTCYRRHPDRTY-EYHHHGHAEFGRQHVQYPMIQH 283
R++ H + +H D + E+HHH H R+H ++H
Sbjct: 735 RSHHHRSRKHEKHRDSSDDEHHHHRHRSSRRKHEDSSDVEH 775
>At5g55100.1 68418.m06868 SWAP (Suppressor-of-White-APricot)/surp
domain-containing protein contains Pfam domain PF01805:
Surp module
Length = 843
Score = 29.1 bits (62), Expect = 2.6
Identities = 12/41 (29%), Positives = 21/41 (51%), Gaps = 1/41 (2%)
Frame = -3
Query: 402 RTYVHHDTCYRRHPDRTY-EYHHHGHAEFGRQHVQYPMIQH 283
R++ H + +H D + E+HHH H R+H ++H
Sbjct: 735 RSHHHRSRKHEKHRDSSDDEHHHHRHRSSRRKHEDSSDVEH 775
>At4g39480.1 68417.m05585 cytochrome P450 family protein contains
Pfam profile: PF00067 cytochrome P450
Length = 989
Score = 28.3 bits (60), Expect = 4.5
Identities = 12/32 (37%), Positives = 20/32 (62%)
Frame = +3
Query: 162 PLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCV 257
P+PF K+P +PD++ H + NSR +C+
Sbjct: 384 PVPFNHKSPAKPDVLPSGH-KVKANSRILFCL 414
Score = 27.5 bits (58), Expect = 7.9
Identities = 11/32 (34%), Positives = 20/32 (62%)
Frame = +3
Query: 162 PLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCV 257
P+PF K+P +PD++ H + NS+ +C+
Sbjct: 857 PVPFQHKSPTKPDVLPSGH-KVDANSKILFCL 887
>At1g26400.1 68414.m03220 FAD-binding domain-containing protein
similar to SP|P30986 reticuline oxidase precursor
(Berberine-bridge-forming enzyme) (BBE)
(Tetrahydroprotoberberine synthase) [Eschscholzia
californica]; contains PF01565 FAD binding domain
Length = 527
Score = 28.3 bits (60), Expect = 4.5
Identities = 13/22 (59%), Positives = 16/22 (72%)
Frame = -2
Query: 592 CLVLLISWTLSATTKGSSGIFS 527
CLVLL+S +A TK SGIF+
Sbjct: 9 CLVLLVSILRAAVTKPDSGIFT 30
>At5g17650.1 68418.m02069 glycine/proline-rich protein
glycine/proline-rich protein GPRP - Arabidopsis
thaliana, EMBL:X84315
Length = 173
Score = 27.9 bits (59), Expect = 6.0
Identities = 10/24 (41%), Positives = 12/24 (50%)
Frame = -3
Query: 390 HHDTCYRRHPDRTYEYHHHGHAEF 319
HH Y H Y Y +HGH +F
Sbjct: 122 HHHGHYGHHHGHGYGYGYHGHGKF 145
>At5g01010.1 68418.m00001 expressed protein
Length = 409
Score = 27.9 bits (59), Expect = 6.0
Identities = 16/45 (35%), Positives = 28/45 (62%), Gaps = 1/45 (2%)
Frame = -1
Query: 287 STGLVTSLLAHAVGLPRVLGHRNVNIIDQVR-TDGRLEHEREGLG 156
+TG+ +L+ + VG+P+VL ++ I Q+ DG +E +RE G
Sbjct: 203 ATGVYKTLVKYLVGVPQVL----LDFIRQINDDDGPMEEQRERYG 243
>At3g12430.1 68416.m01548 expressed protein ; expression supported
by MPSS
Length = 238
Score = 27.9 bits (59), Expect = 6.0
Identities = 12/40 (30%), Positives = 22/40 (55%), Gaps = 2/40 (5%)
Frame = -3
Query: 165 GAWLHPAPSHSSLNTPTLKVGLPID--SFRYFSEAIPLKY 52
G W+H SH+ ++ L VG+ + Y+S + P++Y
Sbjct: 38 GQWIHDVLSHNRFSSHPLVVGVGVQWTPSSYYSASSPVRY 77
>At5g07520.1 68418.m00861 glycine-rich protein (GRP18) Oleosin;
glycine-rich protein 18 (GRP18) PMID:11431566;
Length = 228
Score = 27.5 bits (58), Expect = 7.9
Identities = 15/58 (25%), Positives = 25/58 (43%), Gaps = 3/58 (5%)
Frame = +3
Query: 255 VSKEAGHQTSAESWGTGRAVARIPRVRGGGTHRSGQ---GAFGNMCRGGRMFAPTKPW 419
+ + G + +AE + + + ++P G G G+ G FGN GG F W
Sbjct: 81 IRRRMGAKPTAEGTSSAQPLLKLPVYGGYGGFWGGKKFSGTFGNKPGGGNPFGDISKW 138
>At1g80480.1 68414.m09427 PRLI-interacting factor L, putative
similar to PRLI-interacting factor L [Arabidopsis
thaliana] GI:11139268; contains Pfam profile PF02492:
Cobalamin synthesis protein/P47K
Length = 444
Score = 27.5 bits (58), Expect = 7.9
Identities = 9/28 (32%), Positives = 13/28 (46%)
Frame = -3
Query: 390 HHDTCYRRHPDRTYEYHHHGHAEFGRQH 307
HHD + H D +++ HH H H
Sbjct: 311 HHDHDHDHHHDHNHDHDHHHHDGHDHHH 338
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,771,842
Number of Sequences: 28952
Number of extensions: 283195
Number of successful extensions: 1012
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 941
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 998
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1305036432
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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