BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmnc19c18
(667 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g56510.1 68416.m06284 TBP-binding protein, putative similar t... 138 2e-33
At3g19130.1 68416.m02429 RNA-binding protein, putative similar t... 31 0.69
At5g58470.2 68418.m07323 zinc finger (Ran-binding) family protei... 30 1.2
At5g58470.1 68418.m07322 zinc finger (Ran-binding) family protei... 30 1.2
At1g34140.1 68414.m04235 polyadenylate-binding protein, putative... 30 1.6
At4g00830.1 68417.m00114 RNA recognition motif (RRM)-containing ... 29 2.1
At1g16610.2 68414.m01990 arginine/serine-rich protein, putative ... 29 2.8
At1g16610.1 68414.m01989 arginine/serine-rich protein, putative ... 29 2.8
At1g53720.1 68414.m06113 cyclophilin-RNA interacting protein, pu... 28 6.4
At5g56075.1 68418.m06996 hypothetical protein 27 8.5
At5g55910.1 68418.m06972 protein kinase, putative contains prote... 27 8.5
At5g46250.2 68418.m05694 RNA recognition motif (RRM)-containing ... 27 8.5
At5g46250.1 68418.m05693 RNA recognition motif (RRM)-containing ... 27 8.5
At5g27220.1 68418.m03247 protein transport protein-related low s... 27 8.5
At5g23590.1 68418.m02768 DNAJ heat shock N-terminal domain-conta... 27 8.5
>At3g56510.1 68416.m06284 TBP-binding protein, putative similar to
TBP-binding protein ABT1 GI:6518527 from [Mus musculus]
Length = 257
Score = 138 bits (335), Expect = 2e-33
Identities = 72/163 (44%), Positives = 102/163 (62%), Gaps = 7/163 (4%)
Frame = +2
Query: 128 NQKNIDRTSKFDNKLLKQ--KIRKRGIIFLSTIPPYMNVAKIREIFSQFGEVGRIYLQPT 301
+QK + K KLLK+ K RG+ +LS IPP+M+ ++R I +Q+GE+GRIYL P
Sbjct: 25 SQKADRKKKKLKEKLLKEASKADNRGVCYLSRIPPHMDHVRLRHILAQYGELGRIYLAPE 84
Query: 302 GKPGE-KRKRVPN----QFSEGWVEFEKKKIAKQVAVKLNNTKIGTRKKSRYYDMIWNIK 466
+ RKR +FSEGWVEF KK +AK+VA LN +IG +KKS Y IWNIK
Sbjct: 85 DSEAQVHRKRAGGFRGQRFSEGWVEFAKKSVAKRVADMLNGEQIGGKKKSSVYYDIWNIK 144
Query: 467 YIPRFKWIHLSERLAYERAAMKQRLRAEIAQAKKEAHYLQSNV 595
Y+ +FKW L+E +AY+ A +Q+L ++ AK+E + S +
Sbjct: 145 YLTKFKWDDLTEEIAYKSAIREQKLNMVLSAAKREKDFYLSKI 187
>At3g19130.1 68416.m02429 RNA-binding protein, putative similar to
RNA Binding Protein 47 [Nicotiana plumbaginifolia]
GI:9663769, DNA binding protein ACBF GB:AAC49850 from
[Nicotiana tabacum]; contains InterPro entry IPR000504:
RNA-binding region RNP-1 (RNA recognition motif) (RRM)
Length = 435
Score = 31.1 bits (67), Expect = 0.69
Identities = 25/73 (34%), Positives = 35/73 (47%)
Frame = +2
Query: 203 IFLSTIPPYMNVAKIREIFSQFGEVGRIYLQPTGKPGEKRKRVPNQFSEGWVEFEKKKIA 382
IF+ I P + +R+ FSQFGEV + + P GK G+V+F +K A
Sbjct: 323 IFVGGIDPDVIDEDLRQPFSQFGEVVSVKI-PVGK------------GCGFVQFADRKSA 369
Query: 383 KQVAVKLNNTKIG 421
+ LN T IG
Sbjct: 370 EDAIESLNGTVIG 382
>At5g58470.2 68418.m07323 zinc finger (Ran-binding) family protein
weak similarity to SP|Q01844 RNA-binding protein EWS
(EWS oncogene) (Ewing sarcoma breakpoint region 1
protein) {Homo sapiens}; contains Pfam profiles PF00076:
RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain),
PF00641: Zn-finger in Ran binding protein and others
Length = 422
Score = 30.3 bits (65), Expect = 1.2
Identities = 10/28 (35%), Positives = 20/28 (71%)
Frame = +2
Query: 203 IFLSTIPPYMNVAKIREIFSQFGEVGRI 286
I++S +PP + +++++F G+VGRI
Sbjct: 282 IYISNLPPDVTTDELKDLFGGIGQVGRI 309
>At5g58470.1 68418.m07322 zinc finger (Ran-binding) family protein
weak similarity to SP|Q01844 RNA-binding protein EWS
(EWS oncogene) (Ewing sarcoma breakpoint region 1
protein) {Homo sapiens}; contains Pfam profiles PF00076:
RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain),
PF00641: Zn-finger in Ran binding protein and others
Length = 422
Score = 30.3 bits (65), Expect = 1.2
Identities = 10/28 (35%), Positives = 20/28 (71%)
Frame = +2
Query: 203 IFLSTIPPYMNVAKIREIFSQFGEVGRI 286
I++S +PP + +++++F G+VGRI
Sbjct: 282 IYISNLPPDVTTDELKDLFGGIGQVGRI 309
>At1g34140.1 68414.m04235 polyadenylate-binding protein, putative /
PABP, putative non-consensus splice donor TA at exon 1;
similar to polyadenylate-binding protein
(poly(A)-binding protein) from [Triticum aestivum]
GI:1737492, [Nicotiana tabacum] GI:7673355, {Arabidopsis
thaliana} SP|P42731; contains InterPro entry IPR000504:
RNA-binding region RNP-1 (RNA recognition motif) (RRM)
Length = 407
Score = 29.9 bits (64), Expect = 1.6
Identities = 20/97 (20%), Positives = 46/97 (47%), Gaps = 2/97 (2%)
Frame = +2
Query: 143 DRTSKFD-NKLLKQKIRKRGI-IFLSTIPPYMNVAKIREIFSQFGEVGRIYLQPTGKPGE 316
D +KF+ K+++ ++G+ +++ + ++ K+ E+FS+FG + +
Sbjct: 203 DLKAKFELEKIIRDMKTRKGMNLYVKNLDDSVDNTKLEELFSEFGTITSCKVMVHSNGIS 262
Query: 317 KRKRVPNQFSEGWVEFEKKKIAKQVAVKLNNTKIGTR 427
K G+VEF + A + +K+N +G +
Sbjct: 263 K--------GVGFVEFSTSEEASKAMLKMNGKMVGNK 291
>At4g00830.1 68417.m00114 RNA recognition motif (RRM)-containing
protein similar to nucleolin protein; contains InterPro
entry IPR000504: RNA-binding region RNP-1 (RNA
recognition motif) (RRM)
Length = 495
Score = 29.5 bits (63), Expect = 2.1
Identities = 12/39 (30%), Positives = 25/39 (64%)
Frame = +2
Query: 203 IFLSTIPPYMNVAKIREIFSQFGEVGRIYLQPTGKPGEK 319
+++ IP + +++E+F + GEV +I + P GK G++
Sbjct: 294 LYVKNIPENTSTEQLKELFQRHGEVTKI-VTPPGKGGKR 331
>At1g16610.2 68414.m01990 arginine/serine-rich protein, putative
(SR45) similar to arginine/serine-rich protein
GI:6601502 from [Arabidopsis thaliana]
Length = 407
Score = 29.1 bits (62), Expect = 2.8
Identities = 17/81 (20%), Positives = 43/81 (53%)
Frame = +2
Query: 176 KQKIRKRGIIFLSTIPPYMNVAKIREIFSQFGEVGRIYLQPTGKPGEKRKRVPNQFSEGW 355
K+ +++ ++ + ++ +N A ++EIF FGEV + + ++ +P G+
Sbjct: 91 KKAVQESLVLHVDSLSRNVNEAHLKEIFGNFGEVIHVEIAM-----DRAVNLPR--GHGY 143
Query: 356 VEFEKKKIAKQVAVKLNNTKI 418
VEF+ + A++ + ++ +I
Sbjct: 144 VEFKARADAEKAQLYMDGAQI 164
>At1g16610.1 68414.m01989 arginine/serine-rich protein, putative
(SR45) similar to arginine/serine-rich protein
GI:6601502 from [Arabidopsis thaliana]
Length = 414
Score = 29.1 bits (62), Expect = 2.8
Identities = 17/81 (20%), Positives = 43/81 (53%)
Frame = +2
Query: 176 KQKIRKRGIIFLSTIPPYMNVAKIREIFSQFGEVGRIYLQPTGKPGEKRKRVPNQFSEGW 355
K+ +++ ++ + ++ +N A ++EIF FGEV + + ++ +P G+
Sbjct: 91 KKAVQESLVLHVDSLSRNVNEAHLKEIFGNFGEVIHVEIAM-----DRAVNLPR--GHGY 143
Query: 356 VEFEKKKIAKQVAVKLNNTKI 418
VEF+ + A++ + ++ +I
Sbjct: 144 VEFKARADAEKAQLYMDGAQI 164
>At1g53720.1 68414.m06113 cyclophilin-RNA interacting protein,
putative
Length = 506
Score = 27.9 bits (59), Expect = 6.4
Identities = 19/77 (24%), Positives = 34/77 (44%)
Frame = +2
Query: 200 IIFLSTIPPYMNVAKIREIFSQFGEVGRIYLQPTGKPGEKRKRVPNQFSEGWVEFEKKKI 379
++F+ + P + IFS+FG V + K G+ ++EFE K+
Sbjct: 244 VLFVCKLNPVTEDEDLHTIFSRFGTVVSADVIRDFKTGD-------SLCYAFIEFENKES 296
Query: 380 AKQVAVKLNNTKIGTRK 430
+Q K++N I R+
Sbjct: 297 CEQAYFKMDNALIDDRR 313
>At5g56075.1 68418.m06996 hypothetical protein
Length = 256
Score = 27.5 bits (58), Expect = 8.5
Identities = 11/23 (47%), Positives = 14/23 (60%)
Frame = -1
Query: 475 RDIFNVPNHVIVAGFLSSANFCI 407
R IFN P H +V GF+ SA +
Sbjct: 155 RVIFNFPTHELVRGFMKSARVLV 177
>At5g55910.1 68418.m06972 protein kinase, putative contains protein
kinase domain, Pfam:PF00069
Length = 498
Score = 27.5 bits (58), Expect = 8.5
Identities = 22/87 (25%), Positives = 39/87 (44%), Gaps = 7/87 (8%)
Frame = +2
Query: 80 DVETERVPE-NGTDCEINQKNIDRTSKFDNKLLKQKIRKRGII------FLSTIPPYMNV 238
D+ T + E NGT C K +D+T+ K L + +R I+ FL T+ +
Sbjct: 119 DIGTVHLAELNGTRCYFAMKVMDKTALASRKKLLRAQTEREILQCLDHPFLPTLYSHFET 178
Query: 239 AKIREIFSQFGEVGRIYLQPTGKPGEK 319
K + +F G ++ +PG++
Sbjct: 179 EKFSCLVMEFCPGGDLHTLRQRQPGKR 205
>At5g46250.2 68418.m05694 RNA recognition motif (RRM)-containing
protein contains similarity to RNA-binding protein;
contains InterPro entry IPR000504: RNA-binding region
RNP-1 (RNA recognition motif) (RRM)
Length = 420
Score = 27.5 bits (58), Expect = 8.5
Identities = 18/75 (24%), Positives = 36/75 (48%), Gaps = 6/75 (8%)
Frame = +2
Query: 203 IFLSTIPPYMNVAKIREIFSQFGEVGRIYL------QPTGKPGEKRKRVPNQFSEGWVEF 364
+ + +P + IREIF + G + + + + + K G+K + + +VE+
Sbjct: 195 VLVENLPEDHSNENIREIFGKAGSIKSVSICDPNAVEESEKGGKKENFIRTRL-HAFVEY 253
Query: 365 EKKKIAKQVAVKLNN 409
E + A++ A LNN
Sbjct: 254 ETVEAAEKAAATLNN 268
>At5g46250.1 68418.m05693 RNA recognition motif (RRM)-containing
protein contains similarity to RNA-binding protein;
contains InterPro entry IPR000504: RNA-binding region
RNP-1 (RNA recognition motif) (RRM)
Length = 422
Score = 27.5 bits (58), Expect = 8.5
Identities = 18/75 (24%), Positives = 36/75 (48%), Gaps = 6/75 (8%)
Frame = +2
Query: 203 IFLSTIPPYMNVAKIREIFSQFGEVGRIYL------QPTGKPGEKRKRVPNQFSEGWVEF 364
+ + +P + IREIF + G + + + + + K G+K + + +VE+
Sbjct: 195 VLVENLPEDHSNENIREIFGKAGSIKSVSICDPNAVEESEKGGKKENFIRTRL-HAFVEY 253
Query: 365 EKKKIAKQVAVKLNN 409
E + A++ A LNN
Sbjct: 254 ETVEAAEKAAATLNN 268
>At5g27220.1 68418.m03247 protein transport protein-related low
similarity to SP|P25386 Intracellular protein transport
protein USO1 {Saccharomyces cerevisiae}
Length = 1181
Score = 27.5 bits (58), Expect = 8.5
Identities = 17/50 (34%), Positives = 23/50 (46%)
Frame = -1
Query: 577 VCFLLCLCNFSPQPLFHCSPLVC*SFTEMNPFKSRDIFNVPNHVIVAGFL 428
+C L CL + SP+P S TE +K+ + N V V GFL
Sbjct: 713 ICLLECLMDMSPEPKTEVQVEAIKSVTE---WKNTTLVKAENPVEVLGFL 759
>At5g23590.1 68418.m02768 DNAJ heat shock N-terminal
domain-containing protein low similarity to SP|P39101
CAJ1 protein Saccharomyces cerevisiae; contains Pfam
profile PF00226 DnaJ domain
Length = 296
Score = 27.5 bits (58), Expect = 8.5
Identities = 10/22 (45%), Positives = 17/22 (77%)
Frame = +2
Query: 242 KIREIFSQFGEVGRIYLQPTGK 307
++RE+FS+FGEV + ++ T K
Sbjct: 190 RLREVFSEFGEVEDVVIRSTKK 211
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,132,691
Number of Sequences: 28952
Number of extensions: 241595
Number of successful extensions: 759
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 736
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 757
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1403159472
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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