BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmnc19b22
(628 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g26870.1 68417.m03867 aspartyl-tRNA synthetase, putative / as... 53 2e-07
At4g31180.2 68417.m04427 aspartyl-tRNA synthetase, putative / as... 51 7e-07
At4g31180.1 68417.m04426 aspartyl-tRNA synthetase, putative / as... 51 7e-07
At4g22430.1 68417.m03241 hypothetical protein 27 7.7
>At4g26870.1 68417.m03867 aspartyl-tRNA synthetase, putative /
aspartate--tRNA ligase, putative simialr to
aspartate-tRNA ligase (EC 6.1.1.12) from Drosophila
melanogaster GI:4512034, Homo sapiens SP|P14868, Rattus
norvegicus SP|P15178; contains Pfam profile PF00152 tRNA
synthetases class II (D, K and N)
Length = 532
Score = 52.8 bits (121), Expect = 2e-07
Identities = 29/97 (29%), Positives = 50/97 (51%)
Frame = +1
Query: 337 SMESEPDFSEGLYGNMKMIQSSGEHRDRVYTDVENLSIKMDGQTVWVRARLQTSRAKGKQ 516
S+E E + YG++ + + TDV NL ++ G V +R RL +R G +
Sbjct: 44 SLEEEDESCSSNYGDVTTNELQSAVEGKELTDVSNLVEEIVGSEVSIRGRLHKNRLVGTK 103
Query: 517 CFAVLRQTSSTVQLLVSVNEQRKISKQMVKFTGNITR 627
F +LR++ TVQ +V E+ ++ M+KF ++R
Sbjct: 104 LFVILRESGFTVQCVV---EETRVGANMIKFVKQLSR 137
>At4g31180.2 68417.m04427 aspartyl-tRNA synthetase, putative /
aspartate--tRNA ligase, putative similar to
Aspartyl-tRNA synthetase (Aspartate--tRNA ligase)
(AspRS) [Homo sapiens] GI:20178330
Length = 558
Score = 50.8 bits (116), Expect = 7e-07
Identities = 36/109 (33%), Positives = 60/109 (55%), Gaps = 12/109 (11%)
Frame = +1
Query: 337 SMESEPDFSEGLYGNMKM--IQSSGEHR---------DRVYTDVENLSIKMDGQTVWVRA 483
S+E +FS YG++ + +QSS + + + +TDV +L +M V +R
Sbjct: 56 SLEENDEFSNN-YGDVTLTELQSSADPKAGKWIEAVEGKEWTDVSDLVEEMLESEVLIRG 114
Query: 484 RLQTSR-AKGKQCFAVLRQTSSTVQLLVSVNEQRKISKQMVKFTGNITR 627
R+ T+R K F VLR++ STVQ +VS +E+ K+ MVK+ ++R
Sbjct: 115 RVHTNRPTSNKLGFVVLRESGSTVQCVVSQSEKTKVGANMVKYLKQLSR 163
>At4g31180.1 68417.m04426 aspartyl-tRNA synthetase, putative /
aspartate--tRNA ligase, putative similar to
Aspartyl-tRNA synthetase (Aspartate--tRNA ligase)
(AspRS) [Homo sapiens] GI:20178330
Length = 558
Score = 50.8 bits (116), Expect = 7e-07
Identities = 36/109 (33%), Positives = 60/109 (55%), Gaps = 12/109 (11%)
Frame = +1
Query: 337 SMESEPDFSEGLYGNMKM--IQSSGEHR---------DRVYTDVENLSIKMDGQTVWVRA 483
S+E +FS YG++ + +QSS + + + +TDV +L +M V +R
Sbjct: 56 SLEENDEFSNN-YGDVTLTELQSSADPKAGKWIEAVEGKEWTDVSDLVEEMLESEVLIRG 114
Query: 484 RLQTSR-AKGKQCFAVLRQTSSTVQLLVSVNEQRKISKQMVKFTGNITR 627
R+ T+R K F VLR++ STVQ +VS +E+ K+ MVK+ ++R
Sbjct: 115 RVHTNRPTSNKLGFVVLRESGSTVQCVVSQSEKTKVGANMVKYLKQLSR 163
>At4g22430.1 68417.m03241 hypothetical protein
Length = 348
Score = 27.5 bits (58), Expect = 7.7
Identities = 16/62 (25%), Positives = 26/62 (41%), Gaps = 3/62 (4%)
Frame = -1
Query: 442 NFQHQCKPCHDVPLTIGSFSYSHKVLLRSQALIPLMAELMKPYARLSFV---VSQRFWQL 272
+F + K CH +PL + + + L S + + L +P L V + WQL
Sbjct: 168 DFYGKPKDCHFIPLPDHALNRNKTCLATSSGFVMYIKTLAQPGGNLLKVWRLIDDSAWQL 227
Query: 271 FW 266
W
Sbjct: 228 MW 229
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,379,714
Number of Sequences: 28952
Number of extensions: 200307
Number of successful extensions: 484
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 480
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 484
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1275599520
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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