Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmnc19b05
(700 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g18020.1 68415.m02094 60S ribosomal protein L8 (RPL8A) 359 e-99
At4g36130.1 68417.m05142 60S ribosomal protein L8 (RPL8C) riboso... 358 2e-99
At3g51190.1 68416.m05604 60S ribosomal protein L8 (RPL8B) riboso... 355 2e-98
At2g44065.2 68415.m05480 ribosomal protein L2 family protein sim... 78 5e-15
At2g44065.1 68415.m05479 ribosomal protein L2 family protein sim... 78 5e-15
At4g14250.1 68417.m02198 UBX domain-containing protein low simil... 48 5e-06
At3g27500.1 68416.m03438 DC1 domain-containing protein contains ... 32 0.32
At4g00450.1 68417.m00062 expressed protein 30 1.7
At2g41230.1 68415.m05091 expressed protein 29 2.2
At5g20860.1 68418.m02477 pectinesterase family protein contains ... 29 3.9
At3g12000.1 68416.m01486 S-locus related protein SLR1, putative ... 29 3.9
At5g65290.1 68418.m08212 LMBR1 integral membrane family protein ... 28 5.2
At4g33410.1 68417.m04748 signal peptide peptidase family protein... 27 9.0
At3g16950.1 68416.m02166 dihydrolipoamide dehydrogenase 1, plast... 27 9.0
At2g22730.1 68415.m02694 transporter-related low similarity to s... 27 9.0
>At2g18020.1 68415.m02094 60S ribosomal protein L8 (RPL8A)
Length = 258
Score = 359 bits (883), Expect = e-99
Identities = 160/220 (72%), Positives = 188/220 (85%)
Frame = +2
Query: 38 MGRVIRAQRKGAGSVFVSHTKKRKGAPKLRSLDYAERHGYIKGVVKDIIHDPGRGAPLAV 217
MGRVIRAQRKGAGSVF SHT RKG K RSLD+ ER+GY+KGVV +IIHDPGRGAPLA
Sbjct: 1 MGRVIRAQRKGAGSVFKSHTHHRKGPAKFRSLDFGERNGYLKGVVTEIIHDPGRGAPLAR 60
Query: 218 VHFRDPYKFKTRKELFIAPEGLYTGQFVYCGKKATLEVGNVMPVGAMPEGTIVCNLEEKM 397
V FR P++FK +KELF+A EG+YTGQF+YCGKKATL VGNV+P+ ++PEG +VCN+E +
Sbjct: 61 VTFRHPFRFKKQKELFVAAEGMYTGQFLYCGKKATLVVGNVLPLRSIPEGAVVCNVEHHV 120
Query: 398 GDRGRLARASGNFATVIGHNPDAKRTRVKLPSGAKKVLPSSNRGMVGIVAGGGRIDKPIL 577
GDRG LARASG++A VI HNPD+ TR+KLPSG+KK++PS R M+G VAGGGR +KP+L
Sbjct: 121 GDRGVLARASGDYAIVIAHNPDSDTTRIKLPSGSKKIVPSGCRAMIGQVAGGGRTEKPML 180
Query: 578 KAGRAYHKYKVKRNCWPYVRGVAMNPVXHPHGGGNHQHIG 697
KAG AYHKY+VKRN WP VRGVAMNPV HPHGGGNHQHIG
Sbjct: 181 KAGNAYHKYRVKRNSWPKVRGVAMNPVEHPHGGGNHQHIG 220
>At4g36130.1 68417.m05142 60S ribosomal protein L8 (RPL8C) ribosomal
protein L8, cytosolic, tomato, PIR1:R5TOL8
Length = 258
Score = 358 bits (881), Expect = 2e-99
Identities = 158/220 (71%), Positives = 187/220 (85%)
Frame = +2
Query: 38 MGRVIRAQRKGAGSVFVSHTKKRKGAPKLRSLDYAERHGYIKGVVKDIIHDPGRGAPLAV 217
MGRVIRAQRKGAGSVF SHT RKG K RSLD+ ER+GY+KGVV +IIHDPGRGAPLA
Sbjct: 1 MGRVIRAQRKGAGSVFKSHTHHRKGPAKFRSLDFGERNGYLKGVVTEIIHDPGRGAPLAR 60
Query: 218 VHFRDPYKFKTRKELFIAPEGLYTGQFVYCGKKATLEVGNVMPVGAMPEGTIVCNLEEKM 397
V FR P++FK +KELF+A EG+YTGQF+YCGKKATL VGNV+P+ ++PEG ++CN+E +
Sbjct: 61 VAFRHPFRFKKQKELFVAAEGMYTGQFLYCGKKATLVVGNVLPLRSIPEGAVICNVEHHV 120
Query: 398 GDRGRLARASGNFATVIGHNPDAKRTRVKLPSGAKKVLPSSNRGMVGIVAGGGRIDKPIL 577
GDRG ARASG++A VI HNPD +R+KLPSG+KK++PS R M+G VAGGGR +KP+L
Sbjct: 121 GDRGVFARASGDYAIVIAHNPDNDTSRIKLPSGSKKIVPSGCRAMIGQVAGGGRTEKPML 180
Query: 578 KAGRAYHKYKVKRNCWPYVRGVAMNPVXHPHGGGNHQHIG 697
KAG AYHKY+VKRNCWP VRGVAMNPV HPHGGGNHQHIG
Sbjct: 181 KAGNAYHKYRVKRNCWPKVRGVAMNPVEHPHGGGNHQHIG 220
>At3g51190.1 68416.m05604 60S ribosomal protein L8 (RPL8B) ribosomal
protein L8, cytosolic - Arabidopsis thaliana, PIR:T04582
Length = 260
Score = 355 bits (873), Expect = 2e-98
Identities = 158/221 (71%), Positives = 187/221 (84%), Gaps = 1/221 (0%)
Frame = +2
Query: 38 MGRVIRAQRKGA-GSVFVSHTKKRKGAPKLRSLDYAERHGYIKGVVKDIIHDPGRGAPLA 214
MGRVIRAQRKGA GSVF SHT RKG K RSLDY ER+GY+KG+V +IIHDPGRGAPLA
Sbjct: 1 MGRVIRAQRKGAAGSVFKSHTHHRKGPAKFRSLDYGERNGYLKGLVTEIIHDPGRGAPLA 60
Query: 215 VVHFRDPYKFKTRKELFIAPEGLYTGQFVYCGKKATLEVGNVMPVGAMPEGTIVCNLEEK 394
V FR P+++ +KELF+A EG+YTGQ++YCGKKA L VGNV+P+G++PEG ++CN+E
Sbjct: 61 RVAFRHPFRYMKQKELFVAAEGMYTGQYLYCGKKANLMVGNVLPLGSIPEGAVICNVELH 120
Query: 395 MGDRGRLARASGNFATVIGHNPDAKRTRVKLPSGAKKVLPSSNRGMVGIVAGGGRIDKPI 574
+GDRG LARASG++A VI HNP++ TRVKLPSG+KK+LPS+ R M+G VAGGGR +KP
Sbjct: 121 VGDRGALARASGDYAIVIAHNPESNTTRVKLPSGSKKILPSACRAMIGQVAGGGRTEKPF 180
Query: 575 LKAGRAYHKYKVKRNCWPYVRGVAMNPVXHPHGGGNHQHIG 697
LKAG AYHKYK KRNCWP VRGVAMNPV HPHGGGNHQHIG
Sbjct: 181 LKAGNAYHKYKAKRNCWPVVRGVAMNPVEHPHGGGNHQHIG 221
>At2g44065.2 68415.m05480 ribosomal protein L2 family protein
similar to ribosomal protein L2 [Gossypium arboreum]
GI:17644114; contains Pfam profile PF03947: Ribosomal
Proteins L2, C-terminal domain
Length = 214
Score = 78.2 bits (184), Expect = 5e-15
Identities = 45/117 (38%), Positives = 66/117 (56%)
Frame = +2
Query: 329 VGNVMPVGAMPEGTIVCNLEEKMGDRGRLARASGNFATVIGHNPDAKRTRVKLPSGAKKV 508
+G+ MP+G M GTI+ N+E G ++ RA+G A ++ P + +KLPSG K
Sbjct: 59 IGSSMPLGMMRIGTIIHNIEMNPGQGAKMVRAAGTNAKIL-KEPAKGKCLIKLPSGDTKW 117
Query: 509 LPSSNRGMVGIVAGGGRIDKPILKAGRAYHKYKVKRNCWPYVRGVAMNPVXHPHGGG 679
+ + R +G V+ K + KAG++ ++ R P VRGVAMNP HPHGGG
Sbjct: 118 INAKCRATIGTVSNPSHGTKKLYKAGQS--RWLGIR---PKVRGVAMNPCDHPHGGG 169
>At2g44065.1 68415.m05479 ribosomal protein L2 family protein
similar to ribosomal protein L2 [Gossypium arboreum]
GI:17644114; contains Pfam profile PF03947: Ribosomal
Proteins L2, C-terminal domain
Length = 214
Score = 78.2 bits (184), Expect = 5e-15
Identities = 45/117 (38%), Positives = 66/117 (56%)
Frame = +2
Query: 329 VGNVMPVGAMPEGTIVCNLEEKMGDRGRLARASGNFATVIGHNPDAKRTRVKLPSGAKKV 508
+G+ MP+G M GTI+ N+E G ++ RA+G A ++ P + +KLPSG K
Sbjct: 59 IGSSMPLGMMRIGTIIHNIEMNPGQGAKMVRAAGTNAKIL-KEPAKGKCLIKLPSGDTKW 117
Query: 509 LPSSNRGMVGIVAGGGRIDKPILKAGRAYHKYKVKRNCWPYVRGVAMNPVXHPHGGG 679
+ + R +G V+ K + KAG++ ++ R P VRGVAMNP HPHGGG
Sbjct: 118 INAKCRATIGTVSNPSHGTKKLYKAGQS--RWLGIR---PKVRGVAMNPCDHPHGGG 169
>At4g14250.1 68417.m02198 UBX domain-containing protein low
similarity to 60S ribosomal protein L2 [Nicotiana
tabacum] GI:9230281; contains Pfam profile PF00789: UBX
domain
Length = 724
Score = 48.4 bits (110), Expect = 5e-06
Identities = 30/67 (44%), Positives = 37/67 (55%), Gaps = 1/67 (1%)
Frame = +2
Query: 479 VKLPSGAKKVLPSSNRGMVGIVAGGGRIDKPILKAGRAYHKYKVKRNCWPYVRGVA-MNP 655
+ LP +KK + S R M+G +A G + K K +KRN W VRGVA MNP
Sbjct: 382 INLPLDSKKTVLSGCRVMIGQIASSG-----LTK------KLMIKRNMWAKVRGVAMMNP 430
Query: 656 VXHPHGG 676
V HPHGG
Sbjct: 431 VEHPHGG 437
>At3g27500.1 68416.m03438 DC1 domain-containing protein contains
Pfam profile PF03107: DC1 domain
Length = 609
Score = 32.3 bits (70), Expect = 0.32
Identities = 21/54 (38%), Positives = 28/54 (51%), Gaps = 1/54 (1%)
Frame = -1
Query: 388 FKIAHNGTLRHSSNRH-HISNFKSCFLSTINKLACVEPFGSNEELLPCLELVWI 230
FK + T+ H S+RH H+S K S+ K AC P GS+ L C E +I
Sbjct: 363 FKRNDDNTIDHFSHRHNHMSLDKGGEESSFCK-ACAHPIGSSSSLYKCSECSFI 415
>At4g00450.1 68417.m00062 expressed protein
Length = 2124
Score = 29.9 bits (64), Expect = 1.7
Identities = 18/79 (22%), Positives = 37/79 (46%), Gaps = 1/79 (1%)
Frame = -1
Query: 400 THFLFKIAHNGTLRHSSNRHHISNFKSCFLSTINKLACV-EPFGSNEELLPCLELVWIAE 224
TH+L K+ GT++ S + ++ + + C+ + G+ +E P L ++
Sbjct: 1149 THYLKKLIGTGTMKASLAEKNDDGYQVAQQIVVGLMDCIRQTGGAAQEGDPSLVSSAVSA 1208
Query: 223 VYNSQRCTSTRVMDYILNN 167
+ NS + R+ D+ L N
Sbjct: 1209 IINSVGLSVARITDFSLGN 1227
>At2g41230.1 68415.m05091 expressed protein
Length = 88
Score = 29.5 bits (63), Expect = 2.2
Identities = 22/54 (40%), Positives = 27/54 (50%)
Frame = -2
Query: 678 PPP*GCXTGFMATPRTYGQQLRLTLYLWYALPAFKIGLSIRPPPATIPTMPLLL 517
P P G + T R+ L L+L L LP F L PPPAT+ +PLLL
Sbjct: 14 PKPMGLNGSSLITARSVALLLFLSLLL-LILPPFLPPLP--PPPATLLLLPLLL 64
>At5g20860.1 68418.m02477 pectinesterase family protein contains
Pfam profile: PF01095 pectinesterase
Length = 512
Score = 28.7 bits (61), Expect = 3.9
Identities = 20/92 (21%), Positives = 37/92 (40%), Gaps = 4/92 (4%)
Frame = +2
Query: 335 NVMPVGAMPEGTIVCNLEEKMGDRGRLARASGNFATVIGHNPDAKRTRVKLP----SGAK 502
+++ G + + ++ +KM RL S I NP K LP +G +
Sbjct: 160 SILDSGGSSSASAISHISQKMDHLSRLVSNSLTLVDTIMKNPKPKTKSTALPRWVTAGER 219
Query: 503 KVLPSSNRGMVGIVAGGGRIDKPILKAGRAYH 598
++L R V + G + +++A A H
Sbjct: 220 RLLVGRARAHVVVAKDGSGDYRTVMEAVTAAH 251
>At3g12000.1 68416.m01486 S-locus related protein SLR1, putative
(S1) identical to S-locus related protein SLR1 homolog
(AtS1) GI:246209 Arabidopsis thaliana]; contains Pfam
profiles PF01453: Lectin (probable mannose binding),
PF00954: S-locus glycoprotein family
Length = 439
Score = 28.7 bits (61), Expect = 3.9
Identities = 25/86 (29%), Positives = 42/86 (48%), Gaps = 2/86 (2%)
Frame = -1
Query: 598 VVCPSS-FQNRFVNTSTSSNNTDHASVA*WQNLLGSRR*LYSCTLSIRIVSNHSGEVSRG 422
+V P + F+ F T+TSS N DH + W + R ++ V+N +S+
Sbjct: 49 IVSPGNIFELGFFKTTTSSRNGDHWYLGIWYKSISERTYVW--------VANRDNPLSK- 99
Query: 421 TCQTTSITHF-LFKIAHNGTLRHSSN 347
+ T I++ L + H+GTL S+N
Sbjct: 100 SIGTLKISYANLVLLDHSGTLVWSTN 125
>At5g65290.1 68418.m08212 LMBR1 integral membrane family protein
contains Pfam PF04791: LMBR1-like conserved region
Length = 733
Score = 28.3 bits (60), Expect = 5.2
Identities = 8/21 (38%), Positives = 13/21 (61%)
Frame = +1
Query: 547 WRWTY*QTYFESWKGIPQVQG 609
W W+Y T+ +W +P +QG
Sbjct: 74 WSWSYWSTFLLTWAVVPLIQG 94
>At4g33410.1 68417.m04748 signal peptide peptidase family protein
contains Pfam domain PF04258: Membrane protein of
unknown function (DUF435)
Length = 372
Score = 27.5 bits (58), Expect = 9.0
Identities = 9/13 (69%), Positives = 11/13 (84%)
Frame = -2
Query: 603 YLWYALPAFKIGL 565
Y+WYALP + IGL
Sbjct: 301 YIWYALPGYAIGL 313
>At3g16950.1 68416.m02166 dihydrolipoamide dehydrogenase 1,
plastidic / lipoamide dehydrogenase 1 (PTLPD1) identical
to plastidic lipoamide dehydrogenase from Arabidopsis
thaliana [gi:7159282]
Length = 570
Score = 27.5 bits (58), Expect = 9.0
Identities = 12/38 (31%), Positives = 18/38 (47%)
Frame = -1
Query: 439 GEVSRGTCQTTSITHFLFKIAHNGTLRHSSNRHHISNF 326
G+V GTC +A +G +R N HH+ +F
Sbjct: 117 GDVVGGTCVNRGCVPSKALLAVSGRMRELQNEHHMKSF 154
>At2g22730.1 68415.m02694 transporter-related low similarity to
spinster membrane proteins from [Drosophila
melanogaster] GI:12003974, GI:12003976, GI:12003972,
GI:12003970; contains Pfam profile PF00083: major
facilitator superfamily protein
Length = 510
Score = 27.5 bits (58), Expect = 9.0
Identities = 24/79 (30%), Positives = 31/79 (39%)
Frame = -2
Query: 546 ATIPTMPLLLDGRTFLAPDGSFTLVRLASGLCPITVAKFPEARARRPLSPIFSSRLHTMV 367
ATIP LL G TFL FT L S I + E +P+ LH +
Sbjct: 361 ATIPNAFKLLSGATFLGAVFCFTAFTLKSLYGFIALFALGELLVFATQAPVNYVCLHCVK 420
Query: 366 PSGIAPTGITFPTSRVAFF 310
PS + P + T + F
Sbjct: 421 PS-LRPLSMAISTVAIHIF 438
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,614,583
Number of Sequences: 28952
Number of extensions: 375211
Number of successful extensions: 1056
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 1021
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1050
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1496852856
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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