Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmnc19a02
(690 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g10950.1 68414.m01257 endomembrane protein 70, putative 172 2e-43
At2g01970.1 68415.m00132 endomembrane protein 70, putative 142 3e-34
At1g14670.1 68414.m01744 endomembrane protein 70, putative simil... 140 1e-33
At5g37310.1 68418.m04481 endomembrane protein 70, putative multi... 124 6e-29
At1g08350.1 68414.m00924 endomembrane protein 70 family protein ... 79 2e-15
At2g24170.1 68415.m02888 endomembrane protein 70, putative simil... 56 2e-08
At3g13772.1 68416.m01738 endomembrane protein 70, putative TM4 f... 48 6e-06
At5g10840.1 68418.m01259 endomembrane protein 70, putative TM4 f... 47 1e-05
At5g25100.1 68418.m02974 endomembrane protein 70, putative TM4 f... 45 4e-05
At4g12650.1 68417.m01990 endomembrane protein 70, putative TM4 f... 29 2.2
At1g12540.1 68414.m01453 basic helix-loop-helix (bHLH) family pr... 29 2.9
At5g35160.1 68418.m04167 endomembrane protein 70, putative p76, ... 28 5.1
At5g26830.1 68418.m03201 threonyl-tRNA synthetase / threonine--t... 28 5.1
At1g04120.1 68414.m00401 ABC transporter family protein Strong s... 28 5.1
At3g51980.1 68416.m05702 expressed protein 28 6.7
At1g55130.1 68414.m06296 endomembrane protein 70, putative simil... 28 6.7
At1g03370.1 68414.m00316 C2 domain-containing protein / GRAM dom... 27 8.9
>At1g10950.1 68414.m01257 endomembrane protein 70, putative
Length = 589
Score = 172 bits (418), Expect = 2e-43
Identities = 85/209 (40%), Positives = 120/209 (57%), Gaps = 6/209 (2%)
Frame = +3
Query: 81 ILFIMWTIVHCDEHTHSYKDGEQVVLWMNTVGPYHNRQETYAYFSLPFCVGTKVTIGHYH 260
+L + T + H Y+ EQV LW+N VGPY+N QETY Y+SLPFC + + H
Sbjct: 14 LLVSLLTPTFASDSDHKYQAEEQVTLWVNKVGPYNNPQETYNYYSLPFCRPSGNNV-HKW 72
Query: 261 ETLSEALQGVELEFSGLDITFKENVPAQQFCAIELNDQTYKAFVYAVKNHYWYQMYIDDL 440
L E L G EL S + I F +NV C +EL++ K F A+++ YW++ ++DDL
Sbjct: 73 GGLGEVLGGNELIDSEIAIKFMKNVERSVICPLELDEAKVKHFKDAIESSYWFEFFMDDL 132
Query: 441 PIWGTVGEIDGDH------FYIWTHKKFDIGYNGNRIVEVNLTAENKERLSPDAKIPFTY 602
P+WG VGE+ D ++THK + YN ++I+ VNLT +N L K+ TY
Sbjct: 133 PLWGFVGELHPDKNSENGKHVLYTHKNIVVKYNKDQIIHVNLTQDNPRPLEAGKKMDLTY 192
Query: 603 EVNWKKSNINFEDRFDKYLDPNFFQHRIH 689
V W +N+ F RFD YLD FF+H+IH
Sbjct: 193 SVQWIPTNVTFARRFDVYLDYPFFEHQIH 221
>At2g01970.1 68415.m00132 endomembrane protein 70, putative
Length = 592
Score = 142 bits (343), Expect = 3e-34
Identities = 72/206 (34%), Positives = 114/206 (55%), Gaps = 12/206 (5%)
Frame = +3
Query: 105 VHCDEHTHSYKDGEQVVLWMNTVGPYHNRQETYAYFSLPFCVGTKVTIGHYHETLSEALQ 284
V D H YKDG+ V L+ N VGP+HN ETY YF LPFC+ V E L E L
Sbjct: 22 VRSDASDHRYKDGDSVPLYANKVGPFHNPSETYRYFDLPFCIPEGVK--DKKEALGEVLN 79
Query: 285 GVELEFSGLDITFKENVPAQQFCAIELNDQTYKAFVYAVKNHYWYQMYIDDLPIWGTVGE 464
G L + + F++ ++ +C +L+ + + F AV+ Y++QMY DDLPIWG +G+
Sbjct: 80 GDRLVSAPYKLNFRDEKDSEVYCKKKLSREEVEHFRRAVEKDYYFQMYYDDLPIWGFIGK 139
Query: 465 IDGD--------HFYIWTHKKFDIGYNGNRIVEVN--LTAENKERLSPDAKI--PFTYEV 608
+D + ++++ H +F+I YN +R++E+N + + L+ D ++ F Y V
Sbjct: 140 VDKESKSDPSEFKYFLYKHIQFEILYNKDRVIEINARMDPHSLVDLTEDKEVDAEFMYTV 199
Query: 609 NWKKSNINFEDRFDKYLDPNFFQHRI 686
WK++ +FE R DKY + H +
Sbjct: 200 KWKETETSFEKRMDKYAMSSSLPHHL 225
>At1g14670.1 68414.m01744 endomembrane protein 70, putative similar
to endomembrane protein emp70 precursor isolog
GB:AAF67014 GI:7677068 (Homo sapiens)
Length = 592
Score = 140 bits (338), Expect = 1e-33
Identities = 74/214 (34%), Positives = 116/214 (54%), Gaps = 12/214 (5%)
Frame = +3
Query: 81 ILFIMWTIVHCDEHTHSYKDGEQVVLWMNTVGPYHNRQETYAYFSLPFCVGTKVTIGHYH 260
ILF V D H YK+G+ V L+ N VGP+HN ETY YF LPFC+ V
Sbjct: 14 ILFSGAGYVRSDASDHRYKEGDTVPLYANKVGPFHNPSETYRYFDLPFCIPEGVK--EKK 71
Query: 261 ETLSEALQGVELEFSGLDITFKENVPAQQFCAIELNDQTYKAFVYAVKNHYWYQMYIDDL 440
E L E L G L + + F++ ++ +C +L+ + K F AV+ Y++QMY DDL
Sbjct: 72 EALGEVLNGDRLVSAPYKLNFRDEKESEVYCNKKLSKEEVKQFRKAVEKDYYFQMYYDDL 131
Query: 441 PIWGTVGEIDGD--------HFYIWTHKKFDIGYNGNRIVEVN--LTAENKERLSPDAKI 590
PIWG +G++D D ++++ H +F+I YN +R++E++ + + L+ D ++
Sbjct: 132 PIWGFIGKVDKDIKSDPSEFKYFLYKHIQFEILYNKDRVIEISARMDPHSLVDLTEDKEV 191
Query: 591 --PFTYEVNWKKSNINFEDRFDKYLDPNFFQHRI 686
F Y V WK++ FE R +KY + H +
Sbjct: 192 DAEFMYTVKWKETETPFEKRMEKYSMSSSLPHHL 225
>At5g37310.1 68418.m04481 endomembrane protein 70, putative
multispanning membrane protein, Homo sapiens,
EMBL:HSU94831
Length = 564
Score = 124 bits (299), Expect = 6e-29
Identities = 66/203 (32%), Positives = 104/203 (51%), Gaps = 12/203 (5%)
Frame = +3
Query: 114 DEHTHSYKDGEQVVLWMNTVGPYHNRQETYAYFSLPFCVGTKVTIGHYHETLSEALQGVE 293
D H YK G+ V L+ N VGP+HN ETY YF LPFC + + E L E L G
Sbjct: 26 DGSDHRYKVGDDVPLYANKVGPFHNPSETYRYFDLPFC--SSAPVKEKKEALGEVLNGDR 83
Query: 294 LEFSGLDITFKENVPAQQFCAIELNDQTYKAFVYAVKNHYWYQMYIDDLPIWGTVGEI-- 467
L + + F ++ C L+ + F + Y++QMY DDLPIWG +G++
Sbjct: 84 LVSAPYKLEFLGEKNSEVACRKRLSREDVAKFRDVIAKDYYFQMYYDDLPIWGFLGKVVK 143
Query: 468 DGD------HFYIWTHKKFDIGYNGNRIVEVNLTAENKERL----SPDAKIPFTYEVNWK 617
+G +Y++ H +F+I YN +R++E+ + + + + ++ FTY V WK
Sbjct: 144 EGKTDPSEYKYYLFNHLQFEIFYNKDRVIEIIVRTDQNFLVDLTEDKEVQVDFTYTVRWK 203
Query: 618 KSNINFEDRFDKYLDPNFFQHRI 686
++ I FE R +KY + H +
Sbjct: 204 ETEIPFEKRMEKYSLASSMPHHL 226
>At1g08350.1 68414.m00924 endomembrane protein 70 family protein
KNOLLE; similar to putative endosomal protein
GB:AAD20090 GI:4406780 from [Arabidopsis thaliana]
Length = 508
Score = 79.4 bits (187), Expect = 2e-15
Identities = 41/140 (29%), Positives = 73/140 (52%), Gaps = 15/140 (10%)
Frame = +3
Query: 315 ITFKENVPAQQFCAIELNDQTYKAFVYAVKNHYWYQMYIDDLPIWGTVGEIDGDHF---- 482
+ F+E+ C L F + Y++QMY DDLP+WG VG+++GD+F
Sbjct: 7 LKFREDKTHFVLCRKRLTSSDIARFRDIIAQDYYFQMYYDDLPLWGFVGKVEGDYFGQGE 66
Query: 483 -----YIWTHKKFDIGYNGNRIVEVNLTAENKERL----SPDAKIPFTYEVNWKKSNINF 635
YI++H KF++ YN ++++E+N ++ + + + + FTY V+W ++
Sbjct: 67 KHTKYYIFSHLKFNVLYNADKVIEINSFSDPSYMVDISENTEIDVQFTYSVSWNLTSERS 126
Query: 636 EDRFDKYLDPNF--FQHRIH 689
E R +KY +F +IH
Sbjct: 127 ETRMNKYSRASFHPISQKIH 146
>At2g24170.1 68415.m02888 endomembrane protein 70, putative similar
to MURA transposase of maize Mutator transposon
Length = 637
Score = 56.4 bits (130), Expect = 2e-08
Identities = 33/111 (29%), Positives = 54/111 (48%)
Frame = +3
Query: 192 QETYAYFSLPFCVGTKVTIGHYHETLSEALQGVELEFSGLDITFKENVPAQQFCAIELND 371
Q Y+Y+SLP+C + E L E L+G +E S +E+ C ++L+
Sbjct: 53 QLPYSYYSLPYCRPEHIVDSA--ENLGEVLRGDRIENSPFVFKMRESQMCAAVCRVKLDK 110
Query: 372 QTYKAFVYAVKNHYWYQMYIDDLPIWGTVGEIDGDHFYIWTHKKFDIGYNG 524
+T KAF + + Y M +D+LP+ V D D+ ++ H F +G G
Sbjct: 111 KTAKAFKEKIADEYRVNMILDNLPLVVPVQRPDQDNVVVYQH-GFHVGLKG 160
Score = 29.9 bits (64), Expect = 1.7
Identities = 11/36 (30%), Positives = 24/36 (66%)
Frame = +3
Query: 552 AENKERLSPDAKIPFTYEVNWKKSNINFEDRFDKYL 659
+E+ + + +I FTY+V++++S + + R+D YL
Sbjct: 231 SESPQEVEEGNEIIFTYDVDFQESEVKWASRWDTYL 266
>At3g13772.1 68416.m01738 endomembrane protein 70, putative TM4
family;
Length = 641
Score = 48.0 bits (109), Expect = 6e-06
Identities = 31/112 (27%), Positives = 51/112 (45%)
Frame = +3
Query: 192 QETYAYFSLPFCVGTKVTIGHYHETLSEALQGVELEFSGLDITFKENVPAQQFCAIELND 371
Q Y Y+ L +C K+ + E L E L+G +E S E+ P + C ++LN
Sbjct: 57 QLPYDYYYLNYCKPPKIL--NNAENLGEVLRGDRIENSVYTFQMLEDQPCKVGCRVKLNA 114
Query: 372 QTYKAFVYAVKNHYWYQMYIDDLPIWGTVGEIDGDHFYIWTHKKFDIGYNGN 527
+ K F + + Y M +D+LP+ DG + H F +G+ G+
Sbjct: 115 DSTKNFKEKIDDEYRANMILDNLPVAVLRQRRDGSQSTTYEH-GFRVGFKGS 165
Score = 30.3 bits (65), Expect = 1.3
Identities = 12/25 (48%), Positives = 19/25 (76%)
Frame = +3
Query: 585 KIPFTYEVNWKKSNINFEDRFDKYL 659
+I FTY+V++K+S I + R+D YL
Sbjct: 246 EIVFTYDVSFKESEIKWASRWDTYL 270
>At5g10840.1 68418.m01259 endomembrane protein 70, putative TM4
family;
Length = 648
Score = 47.2 bits (107), Expect = 1e-05
Identities = 29/113 (25%), Positives = 55/113 (48%)
Frame = +3
Query: 132 YKDGEQVVLWMNTVGPYHNRQETYAYFSLPFCVGTKVTIGHYHETLSEALQGVELEFSGL 311
++ G+++ + +N + Q Y+Y+SLPFC +K+ E L E L+G +E +
Sbjct: 44 FEKGDELKVKVNKLTSIKT-QLPYSYYSLPFCRPSKIVDST--ENLGEVLRGDRIENAPY 100
Query: 312 DITFKENVPAQQFCAIELNDQTYKAFVYAVKNHYWYQMYIDDLPIWGTVGEID 470
+E + L+ +T KAF + + Y M +D+LP+ + +D
Sbjct: 101 SFKMREAQMCNILGRVTLDAKTAKAFKEKIDDEYRVNMILDNLPLVVPIERVD 153
Score = 28.3 bits (60), Expect = 5.1
Identities = 10/38 (26%), Positives = 24/38 (63%)
Frame = +3
Query: 546 LTAENKERLSPDAKIPFTYEVNWKKSNINFEDRFDKYL 659
+++ + + +I FTY+V++++S + + R+D YL
Sbjct: 240 VSSATPQEVEQKKEIIFTYDVDFQESEVKWASRWDTYL 277
>At5g25100.1 68418.m02974 endomembrane protein 70, putative TM4
family;
Length = 644
Score = 45.2 bits (102), Expect = 4e-05
Identities = 29/113 (25%), Positives = 54/113 (47%)
Frame = +3
Query: 132 YKDGEQVVLWMNTVGPYHNRQETYAYFSLPFCVGTKVTIGHYHETLSEALQGVELEFSGL 311
++ G+++ + +N + Q Y+Y+SLPFC K+ E L E L+G +E +
Sbjct: 38 FEKGDELKVKVNKLTSIKT-QLPYSYYSLPFCRPKKIVDST--ENLGEVLRGDRIENAPY 94
Query: 312 DITFKENVPAQQFCAIELNDQTYKAFVYAVKNHYWYQMYIDDLPIWGTVGEID 470
+E + L+ ++ KAF + + Y M +D+LP+ + ID
Sbjct: 95 SFKMREAQMCNVLGRVMLDAKSAKAFKEKIDDEYRVNMILDNLPLVVPIERID 147
Score = 27.9 bits (59), Expect = 6.7
Identities = 10/25 (40%), Positives = 19/25 (76%)
Frame = +3
Query: 585 KIPFTYEVNWKKSNINFEDRFDKYL 659
+I FTY+V++++S + + R+D YL
Sbjct: 249 EIIFTYDVDFQESEVKWASRWDAYL 273
>At4g12650.1 68417.m01990 endomembrane protein 70, putative TM4
family;
Length = 527
Score = 29.5 bits (63), Expect = 2.2
Identities = 14/42 (33%), Positives = 21/42 (50%)
Frame = +3
Query: 534 VEVNLTAENKERLSPDAKIPFTYEVNWKKSNINFEDRFDKYL 659
V L + + + +I FTYEV + KS + R+D YL
Sbjct: 111 VNCPLELDKAQIIKEHERITFTYEVEFVKSETRWPSRWDAYL 152
>At1g12540.1 68414.m01453 basic helix-loop-helix (bHLH) family
protein contains Pfam profile: PF00010 helix-loop-helix
DNA-binding domain
Length = 198
Score = 29.1 bits (62), Expect = 2.9
Identities = 12/34 (35%), Positives = 20/34 (58%)
Frame = +3
Query: 210 FSLPFCVGTKVTIGHYHETLSEALQGVELEFSGL 311
F++ C+ T++ G H SE +G+E+ FS L
Sbjct: 150 FNIVSCISTRLHQGFIHTIASEVEEGIEVYFSEL 183
>At5g35160.1 68418.m04167 endomembrane protein 70, putative p76,
Homo sapiens, EMBL:HSU81006
Length = 627
Score = 28.3 bits (60), Expect = 5.1
Identities = 11/24 (45%), Positives = 19/24 (79%)
Frame = +3
Query: 588 IPFTYEVNWKKSNINFEDRFDKYL 659
I F+YEV++++S+I + R+D YL
Sbjct: 228 IVFSYEVSFEESDIKWPSRWDAYL 251
>At5g26830.1 68418.m03201 threonyl-tRNA synthetase / threonine--tRNA
ligase (THRRS) identical to SP|O04630 Threonyl-tRNA
synthetase, mitochondrial precursor (EC 6.1.1.3)
(Threonine--tRNA ligase) (ThrRS) {Arabidopsis thaliana}
Length = 709
Score = 28.3 bits (60), Expect = 5.1
Identities = 15/77 (19%), Positives = 34/77 (44%), Gaps = 9/77 (11%)
Frame = +3
Query: 312 DITFKENVPAQQFCAIELNDQTYKAFVYAVKNHYWYQMYID-------DLPIWGTVGEID 470
++ F + + + L + Y + +KN YW++ Y + ++ +W T G D
Sbjct: 325 ELFFSHQLSPGSYFFLPLGTRVYNRLMDFIKNQYWHRGYTEVITPNMYNMELWQTSGHAD 384
Query: 471 G--DHFYIWTHKKFDIG 515
D+ + + +K + G
Sbjct: 385 NYKDNMFTFNIEKQEFG 401
>At1g04120.1 68414.m00401 ABC transporter family protein Strong
similarity to MRP-like ABC transporter gb|U92650 from A.
thaliana and canalicular multi-drug resistance protein
gb|L49379 from Rattus norvegicus
Length = 1514
Score = 28.3 bits (60), Expect = 5.1
Identities = 9/36 (25%), Positives = 23/36 (63%)
Frame = +3
Query: 294 LEFSGLDITFKENVPAQQFCAIELNDQTYKAFVYAV 401
++F + + F+E++P + C++ +N + F++AV
Sbjct: 1 MDFIEISLIFREHLPLLELCSVIINLLLFLVFLFAV 36
>At3g51980.1 68416.m05702 expressed protein
Length = 382
Score = 27.9 bits (59), Expect = 6.7
Identities = 15/31 (48%), Positives = 22/31 (70%)
Frame = +1
Query: 187 TDKRHMPIFRSRFALALKSLLDIIMKLYLKL 279
T+K +PIF+ R L LKS++D+I+ L L L
Sbjct: 281 TEKDELPIFKDR--LFLKSVVDLIVVLDLDL 309
>At1g55130.1 68414.m06296 endomembrane protein 70, putative similar
to multispanning membrane protein GI:2276460 from [Homo
sapiens]
Length = 637
Score = 27.9 bits (59), Expect = 6.7
Identities = 12/25 (48%), Positives = 18/25 (72%)
Frame = +3
Query: 585 KIPFTYEVNWKKSNINFEDRFDKYL 659
+I FTY+V +K+S I + R+D YL
Sbjct: 242 EIVFTYDVAFKESVIKWASRWDTYL 266
>At1g03370.1 68414.m00316 C2 domain-containing protein / GRAM
domain-containing protein contains Pfam profiles
PF00168: C2 domain; contains PF02893: GRAM domain;
similar to Chain A, Crystal Structure Of Synaptotagmin
Iii C2aC2B Length(GI:6980525); similar to Synaptotagmin
III (SytIII) (Swiss-Prot:P40748) [Rattus norvegicus]
Length = 1859
Score = 27.5 bits (58), Expect = 8.9
Identities = 13/34 (38%), Positives = 21/34 (61%)
Frame = +1
Query: 280 YKASNWSSAVLILRLRKMCRRSNFVPSNSTIKLT 381
YKA+ ++S L +++ S FVP NS+ K+T
Sbjct: 344 YKAAKFASVGSFLLEKRVKSSSEFVPRNSSSKIT 377
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,138,920
Number of Sequences: 28952
Number of extensions: 329231
Number of successful extensions: 930
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 880
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 915
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1467502800
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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