BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmnc19a01
(685 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g37060.1 68417.m05248 patatin, putative similar to patatin-li... 33 0.18
At5g35695.1 68418.m04268 hypothetical protein 29 3.8
At5g19390.2 68418.m02311 pleckstrin homology (PH) domain-contain... 29 3.8
At5g19390.1 68418.m02310 pleckstrin homology (PH) domain-contain... 29 3.8
At5g64070.1 68418.m08046 phosphatidylinositol 4-kinase (PI4K) ne... 27 8.8
At2g26340.1 68415.m03160 expressed protein 27 8.8
>At4g37060.1 68417.m05248 patatin, putative similar to patatin-like
latex allergen [Hevea brasiliensis][PMID:10589016];
contains patatin domain PF01734
Length = 414
Score = 33.1 bits (72), Expect = 0.18
Identities = 28/84 (33%), Positives = 38/84 (45%), Gaps = 4/84 (4%)
Frame = +3
Query: 243 TFEHADQIQHHAPDSVAK--QGDPLYLH-PHTVLITKSGVIQLIMKSKLPYAIELQEWLL 413
TFE + I H+ V K Q + YL L + + L KS L I+L E +L
Sbjct: 295 TFESSRDIVHYHSSVVFKALQSEDKYLRIDDDTLEGDASTLDLSTKSNLENLIKLGEKML 354
Query: 414 EEVIPQV-LCTGKYAPAVEMDTND 482
+ Q+ + TG Y PA E ND
Sbjct: 355 TNRVMQMNIDTGTYEPAAENINND 378
>At5g35695.1 68418.m04268 hypothetical protein
Length = 211
Score = 28.7 bits (61), Expect = 3.8
Identities = 20/59 (33%), Positives = 26/59 (44%)
Frame = -3
Query: 680 LRNVRHAVGQLRRFAARVFAILPSRFSVASSHNHFVGKQNERSVGFRQICVGHRQFLRQ 504
L N+RH LR R+F I SRF++ S F K + G C FLR+
Sbjct: 89 LFNLRHV--SLRNVIERIFGIFKSRFAIFKSAPPFSYK---KQAGLVLTCAALHNFLRK 142
>At5g19390.2 68418.m02311 pleckstrin homology (PH) domain-containing
protein / RhoGAP domain-containing protein weak
similarity to rho-GTPase activating protein [Homo
sapiens] GI:14245732; contains Pfam profiles PF00169: PH
domain, PF00620: RhoGAP domain
Length = 870
Score = 28.7 bits (61), Expect = 3.8
Identities = 17/47 (36%), Positives = 22/47 (46%)
Frame = +3
Query: 75 QVKIGEFKFGEDTFTLRYVLGDEQPVRFVAKDIASSLKYVNCERAIR 215
Q G F DT +Y+ RF+ +D S+L YVN ER R
Sbjct: 691 QTHFGSFSDARDTH--QYLQNHNPQKRFLQQDFDSTLAYVNHERKQR 735
>At5g19390.1 68418.m02310 pleckstrin homology (PH) domain-containing
protein / RhoGAP domain-containing protein weak
similarity to rho-GTPase activating protein [Homo
sapiens] GI:14245732; contains Pfam profiles PF00169: PH
domain, PF00620: RhoGAP domain
Length = 822
Score = 28.7 bits (61), Expect = 3.8
Identities = 17/47 (36%), Positives = 22/47 (46%)
Frame = +3
Query: 75 QVKIGEFKFGEDTFTLRYVLGDEQPVRFVAKDIASSLKYVNCERAIR 215
Q G F DT +Y+ RF+ +D S+L YVN ER R
Sbjct: 691 QTHFGSFSDARDTH--QYLQNHNPQKRFLQQDFDSTLAYVNHERKQR 735
>At5g64070.1 68418.m08046 phosphatidylinositol 4-kinase (PI4K)
nearly identical to gi:4467359
Length = 1121
Score = 27.5 bits (58), Expect = 8.8
Identities = 15/34 (44%), Positives = 20/34 (58%), Gaps = 1/34 (2%)
Frame = +3
Query: 249 EH-ADQIQHHAPDSVAKQGDPLYLHPHTVLITKS 347
EH A Q+ H D + G PL+L P+ VL+T S
Sbjct: 872 EHLAVQLISHFYDIFQEAGLPLWLRPYEVLVTSS 905
>At2g26340.1 68415.m03160 expressed protein
Length = 230
Score = 27.5 bits (58), Expect = 8.8
Identities = 19/54 (35%), Positives = 27/54 (50%)
Frame = +3
Query: 345 SGVIQLIMKSKLPYAIELQEWLLEEVIPQVLCTGKYAPAVEMDTNDVIAKIDDL 506
S ++MKS+L A+ + LL+ V QVL GK V ++ A DDL
Sbjct: 165 SNATVVLMKSQLGTALTALDSLLQTVPSQVLDKGKAMVEVYRSASEEDAGSDDL 218
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,749,481
Number of Sequences: 28952
Number of extensions: 351977
Number of successful extensions: 902
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 882
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 902
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1447936096
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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