Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmnc18e22
(636 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC1450.04 |tef5||translation elongation factor EF-1 beta subun... 73 5e-14
SPBC887.02 |||ClC chloride channel|Schizosaccharomyces pombe|chr... 27 2.3
SPAC23G3.02c |sib1||ferrichrome synthetase Sib1|Schizosaccharomy... 27 3.0
SPBC19C7.02 |ubr1|SPBC32F12.14|N-end-recognizing protein Ubr1|Sc... 26 5.2
SPBC1347.02 |fkbp39||FKBP-type peptidyl-prolyl cis-trans isomera... 25 6.9
SPBC23G7.08c |rga7||GTPase activating protein Rga7|Schizosacchar... 25 6.9
SPAC6G9.14 |||RNA-binding protein|Schizosaccharomyces pombe|chr ... 25 6.9
SPBC646.14c |orc5||origin recognition complex subunit Orc5|Schiz... 25 9.1
SPAC1527.03 |||RNA-binding protein|Schizosaccharomyces pombe|chr... 25 9.1
>SPCC1450.04 |tef5||translation elongation factor EF-1 beta subunit
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 214
Score = 72.5 bits (170), Expect = 5e-14
Identities = 29/47 (61%), Positives = 38/47 (80%)
Frame = +1
Query: 496 QPWDDETDMKEMENQVRTIEMEGLLWGAXKLVPVGYGINKLQIMCVI 636
+PWDDET M E+E VR+I+M+GL+WG KLVPVG+G+NK QI V+
Sbjct: 136 KPWDDETPMDELEKAVRSIQMDGLVWGLSKLVPVGFGVNKFQINLVV 182
Score = 71.3 bits (167), Expect = 1e-13
Identities = 49/144 (34%), Positives = 63/144 (43%), Gaps = 1/144 (0%)
Frame = +2
Query: 68 MAVGDVKTAQGLNDLNQYLAEKSYVSGYTPSQADVQVFEQVGKAP-AANLPHVLRWYNQI 244
M D+ + GL LN +L +KS++ GY PSQAD VF+ VG AP A P+ RWY QI
Sbjct: 1 MGFSDLTSDAGLKQLNDFLLDKSFIEGYEPSQADAVVFKAVGVAPDTAKYPNGARWYKQI 60
Query: 245 ASYTPAERKTWSQGTSPLXXXXXXXXXXXXXXXXXXXXVDLFGSGXXXXXXXXXXXXXXX 424
A+Y A T P +DLFGS
Sbjct: 61 ATYDLA--------TLPGTAKEVSAYGPEGAAAAEEDEIDLFGS-DEEEDPEAERIKAER 111
Query: 425 LKAYADKKSKKPALIAKSSILLDV 496
+ Y KK+ KP + KS + LDV
Sbjct: 112 VAEYNKKKAAKPKAVHKSLVTLDV 135
>SPBC887.02 |||ClC chloride channel|Schizosaccharomyces pombe|chr
2|||Manual
Length = 667
Score = 27.1 bits (57), Expect = 2.3
Identities = 13/37 (35%), Positives = 20/37 (54%)
Frame = -3
Query: 289 GALRPSFAFSRSV*SNLIIPS*YVGKVSGRRLANLLK 179
G L S F ++ + +I+PS +G GR + LLK
Sbjct: 394 GLLLTSATFGAAIPTGIIVPSLAIGACIGRAVGTLLK 430
>SPAC23G3.02c |sib1||ferrichrome synthetase Sib1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 4924
Score = 26.6 bits (56), Expect = 3.0
Identities = 15/53 (28%), Positives = 23/53 (43%)
Frame = +2
Query: 101 LNDLNQYLAEKSYVSGYTPSQADVQVFEQVGKAPAANLPHVLRWYNQIASYTP 259
L+ + YL++ Y T Q + Q+F PA L W N + +Y P
Sbjct: 4648 LDIMINYLSKMYYDDSLTIVQQNSQLFLSTVLDPAVGLSRKKFWNNYLTNYKP 4700
>SPBC19C7.02 |ubr1|SPBC32F12.14|N-end-recognizing protein
Ubr1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1958
Score = 25.8 bits (54), Expect = 5.2
Identities = 13/55 (23%), Positives = 22/55 (40%)
Frame = -1
Query: 597 NRHKFXGSPEKAFHFNSAYLVFHFLHIGFIIPWLTSRRIEDLAMRAGFLDFLSAY 433
++H + AFH +FH + IP + EDL + DF + +
Sbjct: 550 DQHAMIRTDSAAFHSRRYIHIFHHIQFMLSIPCVAEIVREDLKFLKQYADFFNLF 604
>SPBC1347.02 |fkbp39||FKBP-type peptidyl-prolyl cis-trans
isomerase|Schizosaccharomyces pombe|chr 2|||Manual
Length = 361
Score = 25.4 bits (53), Expect = 6.9
Identities = 13/33 (39%), Positives = 16/33 (48%)
Frame = +2
Query: 44 KVATLKETMAVGDVKTAQGLNDLNQYLAEKSYV 142
K TLK + V DVKT G + N E Y+
Sbjct: 251 KTRTLKGGVVVTDVKTGSGASATNGKKVEMRYI 283
>SPBC23G7.08c |rga7||GTPase activating protein
Rga7|Schizosaccharomyces pombe|chr 2|||Manual
Length = 695
Score = 25.4 bits (53), Expect = 6.9
Identities = 11/19 (57%), Positives = 12/19 (63%)
Frame = +3
Query: 300 PPVLNPRLPPQQRKTTMTT 356
PPVL P LPP Q T T+
Sbjct: 449 PPVLLPTLPPIQTTTIQTS 467
>SPAC6G9.14 |||RNA-binding protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 681
Score = 25.4 bits (53), Expect = 6.9
Identities = 18/73 (24%), Positives = 30/73 (41%)
Frame = -3
Query: 604 HTQQAQVXRLPREGLPFQ*CVLGFPFPSYRFHHPMADIKENRGLGNESWFLRLLVSICFQ 425
H AQ+ +L +P ++ F +Y + + N S+FL + ++ Q
Sbjct: 512 HASPAQIEQLVEHIVPHALTLVQDAFGNYVLQYVLELNNPNHTEAIISYFLYKVRALSTQ 571
Query: 424 TFFSNSFSFCILF 386
F SN CI F
Sbjct: 572 KFSSNVMEKCIFF 584
>SPBC646.14c |orc5||origin recognition complex subunit
Orc5|Schizosaccharomyces pombe|chr 2|||Manual
Length = 455
Score = 25.0 bits (52), Expect = 9.1
Identities = 9/27 (33%), Positives = 18/27 (66%)
Frame = +2
Query: 101 LNDLNQYLAEKSYVSGYTPSQADVQVF 181
L+ +++YL ++++ Y PS+ D Q F
Sbjct: 300 LSLVSKYLLVSAFLASYNPSRLDAQFF 326
>SPAC1527.03 |||RNA-binding protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 475
Score = 25.0 bits (52), Expect = 9.1
Identities = 11/27 (40%), Positives = 14/27 (51%)
Frame = -2
Query: 239 DYTIVVRGEG*RPAPCQLAQILEHQLE 159
+Y +VV G G P C + L QLE
Sbjct: 308 NYPVVVNGNGVNPYLCDVQAFLTSQLE 334
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,463,978
Number of Sequences: 5004
Number of extensions: 46426
Number of successful extensions: 132
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 125
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 131
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 283719918
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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