Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmnc18e03
(554 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC1604.05 |pgi1||glucose-6-phosphate isomerase |Schizosaccharo... 165 5e-42
SPAC1D4.12 |rad15|rhp3|transcription factor TFIIH complex subuni... 30 0.20
SPAC3G6.11 |||ATP-dependent DNA helicase Chl1|Schizosaccharomyce... 28 1.1
SPBC3B8.11 |rrn6||RNA polymerase I transcription factor subunit ... 27 1.9
SPCC1259.14c |meu27||S. pombe specific UPF0300 family protein 5|... 25 5.7
SPBC902.02c |ctf18|chl12|DNA replication factor C complex subuni... 25 7.5
SPBC651.08c |rpc1||DNA-directed RNA polymerase III complex large... 25 9.9
SPBC646.09c |int6|yin6|translation initiation factor eIF3e|Schiz... 25 9.9
SPBC1683.11c |||isocitrate lyase|Schizosaccharomyces pombe|chr 2... 25 9.9
>SPBC1604.05 |pgi1||glucose-6-phosphate isomerase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 550
Score = 165 bits (400), Expect = 5e-42
Identities = 82/154 (53%), Positives = 101/154 (65%), Gaps = 2/154 (1%)
Frame = +1
Query: 97 AYQKLQEYYNVNNTKINMLQLFQQDRERFEKFSLCIP--TPNDGDILLDYSKNRINSDVF 270
A+Q +Q +Y + + +LF +D RFEKFS DG IL D+SKN I +
Sbjct: 11 AWQAVQTHYESVGKHLVLKELFAKDSSRFEKFSFTFNGLKEEDGPILFDFSKNLITEETV 70
Query: 271 KLLLDLAKSRNVEQARDAMFSGQKINFTEDRAVLHIALRNRQNKPILVNGKDVSTDVNAV 450
+LL+ LAK NVE RDA+F+G+ INFTEDRAV H ALRN KP+ VNG+DV V V
Sbjct: 71 ELLVKLAKEANVEGLRDALFAGEHINFTEDRAVFHPALRNVSEKPMKVNGQDVMPGVRKV 130
Query: 451 LEHMKEFSDQVVSGQWKGYTGKAITDVINIGIGG 552
L HMKEFSD V SG WKGYTGK I ++N+GIGG
Sbjct: 131 LRHMKEFSDAVRSGAWKGYTGKPIKSIVNVGIGG 164
>SPAC1D4.12 |rad15|rhp3|transcription factor TFIIH complex subunit
Rad15|Schizosaccharomyces pombe|chr 1|||Manual
Length = 772
Score = 30.3 bits (65), Expect = 0.20
Identities = 15/41 (36%), Positives = 26/41 (63%)
Frame = +1
Query: 1 DSLSIGLRHSICKAPVQSIVTMEPKINLKQDAAYQKLQEYY 123
+SLSI L S + +SI+++E K+N + + +KLQ+ Y
Sbjct: 244 ESLSIDLTESSLRKASKSILSLEQKVNEVKQSDSKKLQDEY 284
>SPAC3G6.11 |||ATP-dependent DNA helicase Chl1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 844
Score = 27.9 bits (59), Expect = 1.1
Identities = 16/54 (29%), Positives = 25/54 (46%), Gaps = 2/54 (3%)
Frame = +1
Query: 382 LRNRQN--KPILVNGKDVSTDVNAVLEHMKEFSDQVVSGQWKGYTGKAITDVIN 537
+ NR N KP+ + KD + EH K+ D +SG G +++ IN
Sbjct: 668 ITNRLNAKKPLFIESKDFGDNPLDTFEHYKQSVDAGLSGMLFSVIGGRLSEGIN 721
>SPBC3B8.11 |rrn6||RNA polymerase I transcription factor subunit
Rrn6 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 868
Score = 27.1 bits (57), Expect = 1.9
Identities = 13/40 (32%), Positives = 19/40 (47%)
Frame = +1
Query: 4 SLSIGLRHSICKAPVQSIVTMEPKINLKQDAAYQKLQEYY 123
S L+ ++C AP SI E +IN K +EY+
Sbjct: 316 SFKESLQQALCSAPTSSIAYHEEEINPDNTIYRAKWEEYF 355
>SPCC1259.14c |meu27||S. pombe specific UPF0300 family protein
5|Schizosaccharomyces pombe|chr 3|||Manual
Length = 736
Score = 25.4 bits (53), Expect = 5.7
Identities = 13/41 (31%), Positives = 18/41 (43%)
Frame = +1
Query: 352 TEDRAVLHIALRNRQNKPILVNGKDVSTDVNAVLEHMKEFS 474
T LH+A R Q KP N + V + ++ K FS
Sbjct: 173 TNSNIKLHLAARKYQTKPEKFNENSSISQVTSAIKATKAFS 213
>SPBC902.02c |ctf18|chl12|DNA replication factor C complex subunit
Ctf18|Schizosaccharomyces pombe|chr 2|||Manual
Length = 960
Score = 25.0 bits (52), Expect = 7.5
Identities = 15/64 (23%), Positives = 35/64 (54%), Gaps = 2/64 (3%)
Frame = +1
Query: 304 VEQARDAMFSGQKINF-TEDRAVLHIALR-NRQNKPILVNGKDVSTDVNAVLEHMKEFSD 477
+E + FSG KI+ T +++ + NR + +L+ K ++ +++ V E K+F+
Sbjct: 247 IELDEGSSFSGPKISARTSSGKIIYFPKKKNRHSDGLLLQPKRLADEISQVNEIGKDFNQ 306
Query: 478 QVVS 489
+++
Sbjct: 307 DLLN 310
>SPBC651.08c |rpc1||DNA-directed RNA polymerase III complex large
subunit Rpc1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1405
Score = 24.6 bits (51), Expect = 9.9
Identities = 12/40 (30%), Positives = 18/40 (45%)
Frame = -3
Query: 441 DISADILTVHQDRLVLTVTQSYMQYCPVFCEVYLLT*EHG 322
++ + H D L V + Q PV CE+ + EHG
Sbjct: 202 ELKMHLSKAHDDLNPLKVLNLFKQITPVDCELLGMDPEHG 241
>SPBC646.09c |int6|yin6|translation initiation factor
eIF3e|Schizosaccharomyces pombe|chr 2|||Manual
Length = 501
Score = 24.6 bits (51), Expect = 9.9
Identities = 13/31 (41%), Positives = 18/31 (58%), Gaps = 2/31 (6%)
Frame = +1
Query: 82 LKQDAAY--QKLQEYYNVNNTKINMLQLFQQ 168
L+QD Q LQE+YN+ +I +L F Q
Sbjct: 119 LRQDKGQNLQHLQEHYNITPERIAVLYKFAQ 149
>SPBC1683.11c |||isocitrate lyase|Schizosaccharomyces pombe|chr
2|||Manual
Length = 518
Score = 24.6 bits (51), Expect = 9.9
Identities = 12/30 (40%), Positives = 18/30 (60%), Gaps = 1/30 (3%)
Frame = -3
Query: 324 GISGLFNISALSEIEEQLKNITVDTV-LGI 238
G +G ++ A EEQL+N+T D LG+
Sbjct: 415 GFTGNYDFKAAGFSEEQLRNLTSDMAKLGV 444
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,267,221
Number of Sequences: 5004
Number of extensions: 45659
Number of successful extensions: 134
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 132
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 133
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 231978230
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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