Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmnc18b22
(676 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC1223.06 |tea1|alp8|cell end marker Tea1|Schizosaccharomyces ... 28 1.4
SPBC21.01 |mis17|SPBC776.19|kinetochore protein Mis17|Schizosacc... 27 1.9
SPAC22H12.05c |||fasciclin domain protein |Schizosaccharomyces p... 27 1.9
SPBC23G7.11 |||DNA-3-methyladenine glycosidase Mag2 |Schizosacch... 27 3.3
SPBC725.01 |||aspartate aminotransferase|Schizosaccharomyces pom... 26 4.3
SPCC1322.10 |||conserved fungal protein|Schizosaccharomyces pomb... 26 5.7
SPCC1682.06 |||sequence orphan|Schizosaccharomyces pombe|chr 3||... 26 5.7
SPAC1F3.02c |mkh1||MEK kinase |Schizosaccharomyces pombe|chr 1||... 25 7.6
SPAC22F3.10c |gcs1|apd1|glutamate-cysteine ligase Gcs1 |Schizosa... 25 7.6
SPAC17A5.15c |||glutamate-tRNA ligase |Schizosaccharomyces pombe... 25 7.6
SPAC22H10.09 |||sequence orphan|Schizosaccharomyces pombe|chr 1|... 25 10.0
SPCC1322.12c |bub1||serine/threonine protein kinase Bub1|Schizos... 25 10.0
>SPCC1223.06 |tea1|alp8|cell end marker Tea1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1147
Score = 27.9 bits (59), Expect = 1.4
Identities = 20/60 (33%), Positives = 28/60 (46%)
Frame = +2
Query: 479 DVIAKIDDLTQKLTVANADLAEANRSLILFANEMIVARRDAETARQDCENARRETAQLAN 658
D +KID LT+KL VAN AE N +L A E + + + + +E Q N
Sbjct: 618 DSASKIDSLTEKLKVAN---AEKNAALCEAALEKVPLAKHNKLSDGTFSTPDKENVQSTN 674
>SPBC21.01 |mis17|SPBC776.19|kinetochore protein
Mis17|Schizosaccharomyces pombe|chr 2|||Manual
Length = 441
Score = 27.5 bits (58), Expect = 1.9
Identities = 17/46 (36%), Positives = 24/46 (52%), Gaps = 4/46 (8%)
Frame = +2
Query: 533 DLAEANRSLILF----ANEMIVARRDAETARQDCENARRETAQLAN 658
DL +ANR L ANE + +RD R+D + +R+ QL N
Sbjct: 318 DLLDANRILSTACKKAANEKLAVQRDLSKLREDRLSVQRKKIQLRN 363
>SPAC22H12.05c |||fasciclin domain protein |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 728
Score = 27.5 bits (58), Expect = 1.9
Identities = 9/27 (33%), Positives = 19/27 (70%)
Frame = +2
Query: 332 LITKSGVIQLIMKSKLPYAIELQEWLL 412
L+ K GV+ L+ K KLP+++ ++ ++
Sbjct: 542 LLVKDGVVHLVDKVKLPFSVSQKDMII 568
>SPBC23G7.11 |||DNA-3-methyladenine glycosidase Mag2
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 213
Score = 26.6 bits (56), Expect = 3.3
Identities = 17/52 (32%), Positives = 21/52 (40%)
Frame = +2
Query: 224 DGKYKSTFEHADQIQHHAPDSVAKQGDPLYLHPHTVLITKSGVIQLIMKSKL 379
D YK +H I + V K G P L PH G+I+ I KL
Sbjct: 4 DSDYKRAEKHLSSIDNKWSSLVKKVG-PCTLTPHPEHAPYEGIIRAITSQKL 54
>SPBC725.01 |||aspartate aminotransferase|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 437
Score = 26.2 bits (55), Expect = 4.3
Identities = 19/53 (35%), Positives = 27/53 (50%), Gaps = 5/53 (9%)
Frame = -2
Query: 561 MSDRLASAKSALATVNFCVRSSILAI-----TSFVSISTAGAYLPVHST*GMT 418
+ DRL SA+S T C+ ++ LA T +VS T G + V S G+T
Sbjct: 128 IKDRLVSAQSISGTGALCIAANFLASFYPSKTIYVSDPTWGNHKNVFSRAGLT 180
>SPCC1322.10 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 262
Score = 25.8 bits (54), Expect = 5.7
Identities = 14/37 (37%), Positives = 20/37 (54%)
Frame = +3
Query: 195 IVNGQYVSTSTASTNRRLSMPTKSSTMLQIAWQSRAT 305
I G Y STS +ST+ + P+ SST + S +T
Sbjct: 129 ISGGIYSSTSASSTSSSTATPSSSSTTSSSSSSSSST 165
>SPCC1682.06 |||sequence orphan|Schizosaccharomyces pombe|chr
3|||Manual
Length = 238
Score = 25.8 bits (54), Expect = 5.7
Identities = 18/62 (29%), Positives = 27/62 (43%)
Frame = +2
Query: 449 YAPAVEMDTNDVIAKIDDLTQKLTVANADLAEANRSLILFANEMIVARRDAETARQDCEN 628
YAP+V + I L + + A LA SL+LF + V +D ET + +
Sbjct: 146 YAPSVAKANEGLAKAIAGLAKYVAKAIQGLAHIILSLLLFILGLEVIEQDEETGDVEMSS 205
Query: 629 AR 634
R
Sbjct: 206 MR 207
>SPAC1F3.02c |mkh1||MEK kinase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1116
Score = 25.4 bits (53), Expect = 7.6
Identities = 16/59 (27%), Positives = 29/59 (49%)
Frame = +2
Query: 137 DEQPVRFVAKDIASSLKYVNCERAIRVHVDGKYKSTFEHADQIQHHAPDSVAKQGDPLY 313
+EQ VRFV++ + L Y++ + I H D K + D + + ++K D +Y
Sbjct: 928 EEQLVRFVSRQVLYGLSYLHSKGII--HRDLKADNLLIDFDGVCKISDFGISKHSDNVY 984
>SPAC22F3.10c |gcs1|apd1|glutamate-cysteine ligase Gcs1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 669
Score = 25.4 bits (53), Expect = 7.6
Identities = 14/42 (33%), Positives = 20/42 (47%)
Frame = +2
Query: 134 GDEQPVRFVAKDIASSLKYVNCERAIRVHVDGKYKSTFEHAD 259
GDE V+ D S V+ + ++ GKY+ TF H D
Sbjct: 48 GDEIECIVVSMDDKSKKARVSLRQEDILNALGKYEETFRHVD 89
>SPAC17A5.15c |||glutamate-tRNA ligase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 716
Score = 25.4 bits (53), Expect = 7.6
Identities = 9/21 (42%), Positives = 17/21 (80%)
Frame = +2
Query: 563 LFANEMIVARRDAETARQDCE 625
+FANE+++ + DA++ +QD E
Sbjct: 556 IFANEILIEQADAQSFKQDEE 576
>SPAC22H10.09 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 646
Score = 25.0 bits (52), Expect = 10.0
Identities = 8/14 (57%), Positives = 11/14 (78%)
Frame = -3
Query: 215 HVLPVHNLHILNCW 174
HVLP+ L +LNC+
Sbjct: 405 HVLPIRQLFLLNCY 418
>SPCC1322.12c |bub1||serine/threonine protein kinase
Bub1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1044
Score = 25.0 bits (52), Expect = 10.0
Identities = 10/26 (38%), Positives = 14/26 (53%)
Frame = -3
Query: 257 RHAQTSICTCRRRGHVLPVHNLHILN 180
R A T + R +LPVH LH+ +
Sbjct: 776 RQAMTRLKGLRETNSILPVHQLHMFH 801
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,885,773
Number of Sequences: 5004
Number of extensions: 61386
Number of successful extensions: 182
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 177
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 182
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 309878492
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -