Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmnc17p12
(541 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC17G8.10c |dma1||mitotic spindle checkpoint protein Dma1|Schi... 27 1.3
SPCC550.14 |||vigilin |Schizosaccharomyces pombe|chr 3|||Manual 27 1.3
SPBP16F5.03c |||phosphatidylinositol kinase |Schizosaccharomyces... 27 1.8
SPAC23D3.08 |usp108||U1 snRNP-associated protein Usp108|Schizosa... 27 2.3
SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 27 2.3
SPBC1685.15c |klp6|sot2, SPBC649.01c|kinesin-like protein Klp6|S... 27 2.3
SPBC211.03c |||guanyl-nucleotide exchange factor|Schizosaccharom... 26 4.1
SPBP23A10.14c |ell1||RNA polymerase II transcription elongation ... 25 7.2
SPBC16C6.02c |vps1302|vps13b|chorein homolog|Schizosaccharomyces... 25 7.2
SPAC18G6.04c |shm2||serine hydroxymethyltransferase Shm2 |Schizo... 25 9.5
SPBC27B12.04c |||conserved eukaryotic protein|Schizosaccharomyce... 25 9.5
>SPAC17G8.10c |dma1||mitotic spindle checkpoint protein
Dma1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 267
Score = 27.5 bits (58), Expect = 1.3
Identities = 17/48 (35%), Positives = 23/48 (47%)
Frame = +3
Query: 240 QCVICLR*PISSIAAFPAPCAT*YTTGYKQTSSWLFTAHSSFPCAI*R 383
+C ICL + A F APC+ ++ YK L +H F C I R
Sbjct: 191 ECCICLMPVLPCQALFVAPCS--HSYHYKCIRPTLNESHPYFSCFICR 236
>SPCC550.14 |||vigilin |Schizosaccharomyces pombe|chr 3|||Manual
Length = 1279
Score = 27.5 bits (58), Expect = 1.3
Identities = 15/32 (46%), Positives = 19/32 (59%), Gaps = 1/32 (3%)
Frame = +1
Query: 442 SITKDTGAHIEIST-SKDGSLTFLITGKQSAV 534
++ T I +ST SK + TFLI GK SAV
Sbjct: 179 TVMHQTSTRINVSTASKTKNTTFLIQGKTSAV 210
>SPBP16F5.03c |||phosphatidylinositol kinase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 3699
Score = 27.1 bits (57), Expect = 1.8
Identities = 15/52 (28%), Positives = 23/52 (44%)
Frame = +1
Query: 238 NNVSFAYDDLFPALPHSQPLVQRNIQQATNKLRVGSSLHTQVFHVPYEERKL 393
NN+ A+ F L H V+ +QQA + S L +P ++R L
Sbjct: 1245 NNIINAFSPFFLELLHPNDHVRNTVQQAIENISNNSKLSVVDLLLPIKDRLL 1296
>SPAC23D3.08 |usp108||U1 snRNP-associated protein
Usp108|Schizosaccharomyces pombe|chr 1|||Manual
Length = 382
Score = 26.6 bits (56), Expect = 2.3
Identities = 21/57 (36%), Positives = 32/57 (56%), Gaps = 8/57 (14%)
Frame = +1
Query: 280 PHSQPLVQRNI---QQATNKLRVGSSLHTQVFH-VPYEERKLD---NAN-TFGEGES 426
PHS P + NI Q + K+ V SSL Q++H +P++ ++ NAN T E +S
Sbjct: 248 PHSGPSIPLNIAATQVVSQKMSVPSSLLDQLYHAMPHKLMEISQNTNANITVEESDS 304
>SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit
Spp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2363
Score = 26.6 bits (56), Expect = 2.3
Identities = 11/33 (33%), Positives = 17/33 (51%)
Frame = -1
Query: 211 QHAWVHQDERVSHLQPSWIADASFLSPPAVLVR 113
Q+ W D R HL PSW+ + PP ++ +
Sbjct: 947 QYLWFEADRR--HLFPSWVKPSDSEPPPLLVYK 977
>SPBC1685.15c |klp6|sot2, SPBC649.01c|kinesin-like protein
Klp6|Schizosaccharomyces pombe|chr 2|||Manual
Length = 784
Score = 26.6 bits (56), Expect = 2.3
Identities = 11/24 (45%), Positives = 13/24 (54%)
Frame = +1
Query: 367 HVPYEERKLDNANTFGEGESLRTC 438
HVPY + KL F G + RTC
Sbjct: 338 HVPYRDSKLTRLLKFSLGGNCRTC 361
>SPBC211.03c |||guanyl-nucleotide exchange factor|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1462
Score = 25.8 bits (54), Expect = 4.1
Identities = 12/42 (28%), Positives = 22/42 (52%)
Frame = +1
Query: 142 MMHQQSMMVGDVIPVHPDVPMHVEEMNNVGYENNVSFAYDDL 267
++H + G I V P++P H+ + N + NN+ Y +L
Sbjct: 1396 ILHGAGALAGPTIEVDPEIPDHLIKTWNTTW-NNLFIYYPEL 1436
>SPBP23A10.14c |ell1||RNA polymerase II transcription elongation
factor SpELL|Schizosaccharomyces pombe|chr 2|||Manual
Length = 533
Score = 25.0 bits (52), Expect = 7.2
Identities = 22/73 (30%), Positives = 31/73 (42%), Gaps = 8/73 (10%)
Frame = +1
Query: 181 PVHPDV------PMHVEEMNNVGYENNVSFAYDDLFPALPHSQPLV--QRNIQQATNKLR 336
PV+ DV P + + N V + +F L P P LV Q+NI + N+ R
Sbjct: 248 PVYKDVRVFDWRPYSIADRNAVLSRMSNAFDNMQLPPDAPERSLLVSKQKNITKLNNEKR 307
Query: 337 VGSSLHTQVFHVP 375
+ L HVP
Sbjct: 308 IPPQLAQPTSHVP 320
>SPBC16C6.02c |vps1302|vps13b|chorein homolog|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 3131
Score = 25.0 bits (52), Expect = 7.2
Identities = 11/39 (28%), Positives = 19/39 (48%)
Frame = +2
Query: 326 TNFELALHCTLKFSMCHMKNENLTMLILLVRENP*ELAI 442
TN+ + LHC + + H+ +L+ L P L+I
Sbjct: 1212 TNYAIDLHCGISELLIHLSQAQYLILLKLASNLPEMLSI 1250
>SPAC18G6.04c |shm2||serine hydroxymethyltransferase Shm2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 472
Score = 24.6 bits (51), Expect = 9.5
Identities = 15/53 (28%), Positives = 29/53 (54%), Gaps = 5/53 (9%)
Frame = -2
Query: 165 HHGLLMHHFCLLRQSLSAPSRYSTKVLYQIK----LKDY-AVDARIVQFFPQL 22
H G L H F ++++SA S Y + + Y + + DY +++ +QF P++
Sbjct: 137 HGGHLSHGFSTPQKAISAVSTYFSTMPYNVNKETGIIDYDSLEKAAIQFRPKV 189
>SPBC27B12.04c |||conserved eukaryotic protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 817
Score = 24.6 bits (51), Expect = 9.5
Identities = 24/97 (24%), Positives = 46/97 (47%), Gaps = 2/97 (2%)
Frame = +1
Query: 244 VSFAYDDLFPALPHSQPLVQRNIQQATNKLRVGSSLHTQVFHV--PYEERKLDNANTFGE 417
VS +YDDLFP + + +Q +Q + + + L+ + F +R DNA
Sbjct: 445 VSQSYDDLFPYMDN---FIQLAVQLFYHISKKVNCLYVEAFSSSDAVLQRIQDNAVVDSP 501
Query: 418 GESLRTCHSITKDTGAHIEISTSKDGSLTFLITGKQS 528
E L ++ ++++I ++D + F I+G+ S
Sbjct: 502 TEEL--SEQAARNRKSNVKIEYTEDFATGFAISGEIS 536
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,364,719
Number of Sequences: 5004
Number of extensions: 50640
Number of successful extensions: 148
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 143
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 148
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 221892220
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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