Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmnc15b09
(531 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces po... 78 9e-16
SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces ... 61 1e-10
SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces po... 50 3e-07
SPBC577.08c |txl1|trx3|thioredoxin-like I protein Txl1|Schizosac... 40 3e-04
SPBC12D12.07c |trx2||mitochondrial thioredoxin Trx2|Schizosaccha... 40 3e-04
SPAC959.05c |||protein disulfide isomerase |Schizosaccharomyces ... 36 0.005
SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyc... 35 0.009
SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|c... 32 0.046
SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces ... 30 0.19
SPAC926.09c |fas1||fatty acid synthase beta subunit Fas1|Schizos... 27 1.3
SPAC23E2.03c |ste7||meiotic suppressor protein Ste7|Schizosaccha... 27 1.7
SPCC188.13c |dcr1|SPCC584.10c|dicer|Schizosaccharomyces pombe|ch... 26 3.0
SPBC25H2.16c |||adaptin|Schizosaccharomyces pombe|chr 2|||Manual 26 4.0
SPCC338.17c |rad21||kleisin|Schizosaccharomyces pombe|chr 3|||Ma... 25 5.3
SPBC1683.13c |||transcription factor |Schizosaccharomyces pombe|... 25 5.3
>SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 492
Score = 77.8 bits (183), Expect = 9e-16
Identities = 36/116 (31%), Positives = 67/116 (57%)
Frame = +2
Query: 179 QITQSNLDMVLASNEVVFINFYAEWCKFSNMLMPIFDDAADEVTKAGYDTGKVVMGKVDC 358
++ + L+ ++ +++V+ + FYA WC L P ++ AADE+ K G + + +VDC
Sbjct: 26 KVNKEGLNELITADKVLMVKFYAPWCGHCKALAPEYESAADELEKDG-----ISLVEVDC 80
Query: 359 DKEGAIATRFHITKYPTLKLFRNGLPAKKEYRGQRSVEAFAEFIKKQLVDPIVTFS 526
+EG + + + I YPTL +F+NG +Y G R +A ++++KQL+ + S
Sbjct: 81 TEEGDLCSEYSIRGYPTLNVFKNGKQI-SQYSGPRKHDALVKYMRKQLLPTVKPIS 135
Score = 48.8 bits (111), Expect = 5e-07
Identities = 31/100 (31%), Positives = 49/100 (49%), Gaps = 1/100 (1%)
Frame = +2
Query: 200 DMVLASNEVVFINFYAEWCKFSNMLMPIFDDAADEVTKAGYDTGKVVMGKVDCDKEGAIA 379
D+V+ + V + FYA WC L P ++ A+E + D VV+ K+D E I+
Sbjct: 367 DIVMDETKDVLVEFYAPWCGHCKNLAPTYEKLAEEYS----DDSNVVVAKIDA-TENDIS 421
Query: 380 TRFHITKYPTLKLFR-NGLPAKKEYRGQRSVEAFAEFIKK 496
I+ +PT+ F+ N Y G R++E + FI K
Sbjct: 422 V--SISGFPTIMFFKANDKVNPVRYEGDRTLEDLSAFIDK 459
>SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 359
Score = 60.9 bits (141), Expect = 1e-10
Identities = 33/113 (29%), Positives = 56/113 (49%), Gaps = 2/113 (1%)
Frame = +2
Query: 167 SGAVQITQSNL--DMVLASNEVVFINFYAEWCKFSNMLMPIFDDAADEVTKAGYDTGKVV 340
SG V++ N + + AS + I FYA WC L P+++ E+ D V+
Sbjct: 20 SGVVELQSLNELENTIRASKKGALIEFYATWCGHCKSLAPVYE----ELGALFEDHNDVL 75
Query: 341 MGKVDCDKEGAIATRFHITKYPTLKLFRNGLPAKKEYRGQRSVEAFAEFIKKQ 499
+GK+D D +A ++HIT +PTL F +Y R V++ +F+ ++
Sbjct: 76 IGKIDADTHSDVADKYHITGFPTLIWFPPDGSEPVQYSNARDVDSLTQFVSEK 128
Score = 54.0 bits (124), Expect = 1e-08
Identities = 32/113 (28%), Positives = 54/113 (47%), Gaps = 2/113 (1%)
Frame = +2
Query: 167 SGAVQITQSNLDMVLASNEV-VFINFYAEWCKFSNMLMPIFDDAADEVTKAGYDTGKVVM 343
S V++ N D V+ ++ V + FYA+WC + L P + + + K + V +
Sbjct: 140 SNVVELDSLNFDKVVMDDKKDVLVEFYADWCGYCKRLAPTY----ETLGKVFKNEPNVEI 195
Query: 344 GKVDCDKEGAIATRFHITKYPTLKLFRNGLPAKKE-YRGQRSVEAFAEFIKKQ 499
K++ D I + +PT+K F K E Y G RS+E+ E+I K+
Sbjct: 196 VKINADVFADIGRLHEVASFPTIKFFPKDDKDKPELYEGDRSLESLIEYINKK 248
>SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 363
Score = 49.6 bits (113), Expect = 3e-07
Identities = 32/156 (20%), Positives = 67/156 (42%), Gaps = 5/156 (3%)
Frame = +2
Query: 77 MNWKCPNLFNFKLSSCLFILLCHSFYNPT-DSGAVQITQSNLDMVLASNEVVFINFYAEW 253
M ++ P LF L+ C ++ Y+P S +++ N + + + FYA W
Sbjct: 1 MLFRIPTLFTLFLA-CFSLVSGVFGYSPMFGSNTIELNSKNFRKFVKAKGPSLVVFYAPW 59
Query: 254 CKFSNMLMPIFDDAADEVTKAGYDTGKVVMGKVDCDKEGAIATRFHITKYPTLKLF---- 421
C + L+P + A + + V D D+ A+ +++ + +PT+KL
Sbjct: 60 CGYCKKLVPTYQKLASNL----HSLLPVTAVDCDADQNRAVCSQYQVQGFPTIKLVYPSS 115
Query: 422 RNGLPAKKEYRGQRSVEAFAEFIKKQLVDPIVTFSS 529
+ + +Y G RS ++ +F+ + + +S
Sbjct: 116 KGSSLSSTDYNGDRSYKSLQKFVSDSIPSKVKILTS 151
>SPBC577.08c |txl1|trx3|thioredoxin-like I protein
Txl1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 290
Score = 39.5 bits (88), Expect = 3e-04
Identities = 20/66 (30%), Positives = 32/66 (48%)
Frame = +2
Query: 233 INFYAEWCKFSNMLMPIFDDAADEVTKAGYDTGKVVMGKVDCDKEGAIATRFHITKYPTL 412
++ YA+WC + P+F A + Y + K V KV+ D++ IA+ + PT
Sbjct: 24 VDCYADWCGPCKAISPLFSQLASK-----YASPKFVFAKVNVDEQRQIASGLGVKAMPTF 78
Query: 413 KLFRNG 430
F NG
Sbjct: 79 VFFENG 84
>SPBC12D12.07c |trx2||mitochondrial thioredoxin
Trx2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 121
Score = 39.5 bits (88), Expect = 3e-04
Identities = 21/74 (28%), Positives = 38/74 (51%)
Frame = +2
Query: 209 LASNEVVFINFYAEWCKFSNMLMPIFDDAADEVTKAGYDTGKVVMGKVDCDKEGAIATRF 388
+++++V ++FYA+WC L P + +++ KA + V+ DK IA +
Sbjct: 32 ISADKVTVVDFYADWCGPCKYLKPFLEKLSEQNQKASFIA-------VNADKFSDIAQKN 84
Query: 389 HITKYPTLKLFRNG 430
+ PT+ LFR G
Sbjct: 85 GVYALPTMVLFRKG 98
>SPAC959.05c |||protein disulfide isomerase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 632
Score = 35.5 bits (78), Expect = 0.005
Identities = 12/31 (38%), Positives = 22/31 (70%)
Frame = +2
Query: 332 KVVMGKVDCDKEGAIATRFHITKYPTLKLFR 424
++ M +V+CD+E + FHI K+PT ++F+
Sbjct: 229 RLKMAQVNCDEEKEMCNHFHIKKFPTFRVFQ 259
>SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 103
Score = 34.7 bits (76), Expect = 0.009
Identities = 19/80 (23%), Positives = 38/80 (47%)
Frame = +2
Query: 191 SNLDMVLASNEVVFINFYAEWCKFSNMLMPIFDDAADEVTKAGYDTGKVVMGKVDCDKEG 370
S ++ +++V ++F+A WC + P F+ ++ + A + KVD D+
Sbjct: 9 SEFKSIVCQDKLVVVDFFATWCGPCKAIAPKFEQFSNTYSDATFI-------KVDVDQLS 61
Query: 371 AIATRFHITKYPTLKLFRNG 430
IA + P+ L++NG
Sbjct: 62 EIAAEAGVHAMPSFFLYKNG 81
>SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|chr
2|||Manual
Length = 244
Score = 32.3 bits (70), Expect = 0.046
Identities = 18/66 (27%), Positives = 28/66 (42%)
Frame = +2
Query: 221 EVVFINFYAEWCKFSNMLMPIFDDAADEVTKAGYDTGKVVMGKVDCDKEGAIATRFHITK 400
+++ +NFYA W + +FD A DT V K++ +K IA F +
Sbjct: 21 QIILLNFYAPWAAPCKQMNQVFDQFAK-------DTKNAVFLKIEAEKFSDIAESFDVNA 73
Query: 401 YPTLKL 418
P L
Sbjct: 74 VPLFVL 79
>SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 726
Score = 30.3 bits (65), Expect = 0.19
Identities = 23/95 (24%), Positives = 38/95 (40%), Gaps = 5/95 (5%)
Frame = +2
Query: 155 NPTDSGAVQITQSNLDMVLASNEVVFINFYAEWCKFSNMLMPIFDDAADEVTKAGYD--- 325
NPT + +++D L E FI FY+ C D D+V+ A Y
Sbjct: 277 NPTGTSKALALDADIDAALTDKEGWFIQFYSSEC-----------DDCDDVSTAWYAMAN 325
Query: 326 --TGKVVMGKVDCDKEGAIATRFHITKYPTLKLFR 424
GK+ + ++C ++ I +PT F+
Sbjct: 326 RMRGKLNVAHINCAVSKRACKQYSIQYFPTFLFFK 360
>SPAC926.09c |fas1||fatty acid synthase beta subunit
Fas1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2073
Score = 27.5 bits (58), Expect = 1.3
Identities = 15/25 (60%), Positives = 16/25 (64%)
Frame = -1
Query: 360 SQSTFPITTLPVS*PAFVTSSAASS 286
SQ TFP TTLP P+ V S ASS
Sbjct: 306 SQQTFPTTTLP---PSVVADSLASS 327
>SPAC23E2.03c |ste7||meiotic suppressor protein
Ste7|Schizosaccharomyces pombe|chr 1|||Manual
Length = 569
Score = 27.1 bits (57), Expect = 1.7
Identities = 30/94 (31%), Positives = 45/94 (47%), Gaps = 13/94 (13%)
Frame = -1
Query: 525 LKVTIGSTNCFLINSANASTDL*PLYSFLAGKPLRNSFNVG------YLVMWNRVAIA-- 370
LK TI FL +S +A +L + LAGKPL + + G L ++ V +
Sbjct: 124 LKATISKRYAFLGSSQSAKAELIMVPPNLAGKPLSANSSFGSSAKTFQLPEFDSVTPSAS 183
Query: 369 ----PSLSQSTFPITTLPVS*PA-FVTSSAASSN 283
PS + + PITT + + F TSS++S N
Sbjct: 184 LYKQPSFNSNPAPITTSSATHTSQFSTSSSSSVN 217
>SPCC188.13c |dcr1|SPCC584.10c|dicer|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1374
Score = 26.2 bits (55), Expect = 3.0
Identities = 19/56 (33%), Positives = 27/56 (48%)
Frame = +2
Query: 332 KVVMGKVDCDKEGAIATRFHITKYPTLKLFRNGLPAKKEYRGQRSVEAFAEFIKKQ 499
K V+ +V + + T H+TKY FR+G Y R V A A++I KQ
Sbjct: 1281 KCVIEEVKSSHKTLLNTELHLTKYYGFSFFRHGNIV--AYGKSRKV-ANAKYIMKQ 1333
>SPBC25H2.16c |||adaptin|Schizosaccharomyces pombe|chr 2|||Manual
Length = 533
Score = 25.8 bits (54), Expect = 4.0
Identities = 13/37 (35%), Positives = 19/37 (51%)
Frame = +2
Query: 158 PTDSGAVQITQSNLDMVLASNEVVFINFYAEWCKFSN 268
P + GA+ +T+S V S+ V IN + C F N
Sbjct: 391 PAEVGAISLTESFNSPVSNSSPNVPINNFTSTCAFEN 427
>SPCC338.17c |rad21||kleisin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 628
Score = 25.4 bits (53), Expect = 5.3
Identities = 13/40 (32%), Positives = 20/40 (50%)
Frame = -1
Query: 390 WNRVAIAPSLSQSTFPITTLPVS*PAFVTSSAASSNIGIN 271
W+++ PS S +T + +LP+S SS S G N
Sbjct: 141 WSQLLRTPSRSSNTLELHSLPISSSPSFPSSQLSIEAGRN 180
>SPBC1683.13c |||transcription factor |Schizosaccharomyces pombe|chr
2|||Manual
Length = 618
Score = 25.4 bits (53), Expect = 5.3
Identities = 11/23 (47%), Positives = 15/23 (65%)
Frame = -3
Query: 514 YWIYQLLFNKLCKCFHRPLTSVF 446
+W+ Q+ +LCK HR LT VF
Sbjct: 392 FWLNQV---ELCKIIHRMLTEVF 411
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,107,860
Number of Sequences: 5004
Number of extensions: 42147
Number of successful extensions: 130
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 119
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 122
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 218398248
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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