Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmnc14n15
(531 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC1F7.13c |rpl801|rpl8-1, rpl18, rpk5a, rpl2-1, SPAC21E11.02c|... 242 2e-65
SPBC2F12.07c |rpl802|rpl8-2, rpk37, rpk5b|60S ribosomal protein ... 242 2e-65
SPBC839.04 |rpl803|rpl8-3, rpk5-b, rpkD4|60S ribosomal protein L... 242 2e-65
SPAC23D3.10c |eng2||endo-1,3-beta-glucanase Eng2|Schizosaccharom... 26 4.0
SPCC645.13 |||transcription elongation regulator|Schizosaccharom... 25 7.0
SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subuni... 25 9.3
SPAC15E1.04 |||thymidylate synthase |Schizosaccharomyces pombe|c... 25 9.3
>SPAC1F7.13c |rpl801|rpl8-1, rpl18, rpk5a, rpl2-1, SPAC21E11.02c|60S
ribosomal protein L8|Schizosaccharomyces pombe|chr
1|||Manual
Length = 253
Score = 242 bits (593), Expect = 2e-65
Identities = 110/160 (68%), Positives = 134/160 (83%)
Frame = +3
Query: 51 MGRVIRAQRKGAGSVFVSHTKKRKGAPKLRSLDYAERHGYIKGVVKDIIHDPGRGAPLAV 230
MGRVIRAQRK +G +F +HT+ RKGA +LR+LD+AERHGYI+GVV+ IIHDPGRGAPLA
Sbjct: 1 MGRVIRAQRK-SGGIFQAHTRLRKGAAQLRTLDFAERHGYIRGVVQKIIHDPGRGAPLAK 59
Query: 231 VHFRDPYKFKTRKELFIAPEGLYTGQFVYCGKKATLEVGNVMPVGAMPEGTIVCNLEEKM 410
V FR+PY ++T E F+A EG+YTGQFVYCGK A L VGNV+PVG MPEGTI+ N+EEK
Sbjct: 60 VAFRNPYHYRTDVETFVATEGMYTGQFVYCGKNAALTVGNVLPVGEMPEGTIISNVEEKA 119
Query: 411 GDRGRLARASGNFATVIGHNPDAKRTRVKLPSGAKKVLPS 530
GDRG L R+SGN+ ++GH+ D +TRVKLPSGAKKV+PS
Sbjct: 120 GDRGALGRSSGNYVIIVGHDVDTGKTRVKLPSGAKKVVPS 159
>SPBC2F12.07c |rpl802|rpl8-2, rpk37, rpk5b|60S ribosomal protein
L8|Schizosaccharomyces pombe|chr 2|||Manual
Length = 253
Score = 242 bits (593), Expect = 2e-65
Identities = 110/160 (68%), Positives = 134/160 (83%)
Frame = +3
Query: 51 MGRVIRAQRKGAGSVFVSHTKKRKGAPKLRSLDYAERHGYIKGVVKDIIHDPGRGAPLAV 230
MGRVIRAQRK +G +F +HT+ RKGA +LR+LD+AERHGYI+GVV+ IIHDPGRGAPLA
Sbjct: 1 MGRVIRAQRK-SGGIFQAHTRLRKGAAQLRTLDFAERHGYIRGVVQKIIHDPGRGAPLAK 59
Query: 231 VHFRDPYKFKTRKELFIAPEGLYTGQFVYCGKKATLEVGNVMPVGAMPEGTIVCNLEEKM 410
V FR+PY ++T E F+A EG+YTGQFVYCGK A L VGNV+PVG MPEGTI+ N+EEK
Sbjct: 60 VAFRNPYHYRTDVETFVATEGMYTGQFVYCGKNAALTVGNVLPVGEMPEGTIISNVEEKA 119
Query: 411 GDRGRLARASGNFATVIGHNPDAKRTRVKLPSGAKKVLPS 530
GDRG L R+SGN+ ++GH+ D +TRVKLPSGAKKV+PS
Sbjct: 120 GDRGALGRSSGNYVIIVGHDVDTGKTRVKLPSGAKKVVPS 159
>SPBC839.04 |rpl803|rpl8-3, rpk5-b, rpkD4|60S ribosomal protein
L8|Schizosaccharomyces pombe|chr 2|||Manual
Length = 253
Score = 242 bits (593), Expect = 2e-65
Identities = 110/160 (68%), Positives = 134/160 (83%)
Frame = +3
Query: 51 MGRVIRAQRKGAGSVFVSHTKKRKGAPKLRSLDYAERHGYIKGVVKDIIHDPGRGAPLAV 230
MGRVIRAQRK +G +F +HT+ RKGA +LR+LD+AERHGYI+GVV+ IIHDPGRGAPLA
Sbjct: 1 MGRVIRAQRK-SGGIFQAHTRLRKGAAQLRTLDFAERHGYIRGVVQKIIHDPGRGAPLAK 59
Query: 231 VHFRDPYKFKTRKELFIAPEGLYTGQFVYCGKKATLEVGNVMPVGAMPEGTIVCNLEEKM 410
V FR+PY ++T E F+A EG+YTGQFVYCGK A L VGNV+PVG MPEGTI+ N+EEK
Sbjct: 60 VAFRNPYHYRTDVETFVATEGMYTGQFVYCGKNAALTVGNVLPVGEMPEGTIISNVEEKA 119
Query: 411 GDRGRLARASGNFATVIGHNPDAKRTRVKLPSGAKKVLPS 530
GDRG L R+SGN+ ++GH+ D +TRVKLPSGAKKV+PS
Sbjct: 120 GDRGALGRSSGNYVIIVGHDVDTGKTRVKLPSGAKKVVPS 159
>SPAC23D3.10c |eng2||endo-1,3-beta-glucanase
Eng2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 706
Score = 25.8 bits (54), Expect = 4.0
Identities = 12/33 (36%), Positives = 17/33 (51%)
Frame = -3
Query: 421 PLSPIFSSRLHTMVPSGIAPTGITFPTSRVAFF 323
P++P+F SR H P G+ T FP F+
Sbjct: 11 PINPVFPSRAHPSPPLGL--TSNLFPIQTNKFY 41
>SPCC645.13 |||transcription elongation
regulator|Schizosaccharomyces pombe|chr 3|||Manual
Length = 721
Score = 25.0 bits (52), Expect = 7.0
Identities = 10/26 (38%), Positives = 15/26 (57%)
Frame = +3
Query: 72 QRKGAGSVFVSHTKKRKGAPKLRSLD 149
+R+G+ +HTK+ K APK D
Sbjct: 167 KRRGSVGTTATHTKRSKNAPKTSPKD 192
>SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subunit a
Pol2 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 2199
Score = 24.6 bits (51), Expect = 9.3
Identities = 9/19 (47%), Positives = 15/19 (78%)
Frame = +1
Query: 268 RSSSLLPKGSTQANLFIVE 324
+S+++LP GST LF++E
Sbjct: 1353 KSNAILPHGSTSDKLFLLE 1371
>SPAC15E1.04 |||thymidylate synthase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 625
Score = 24.6 bits (51), Expect = 9.3
Identities = 13/36 (36%), Positives = 20/36 (55%)
Frame = -3
Query: 514 LAPDGSFTLVRLASGLCPITVAKFPEARARRPLSPI 407
+AP + T+ ++A+GLC + RA PL PI
Sbjct: 116 IAPLSANTMAKMANGLCDNLLTSL--IRAWAPLKPI 149
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,274,115
Number of Sequences: 5004
Number of extensions: 46966
Number of successful extensions: 131
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 126
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 128
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 218398248
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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