Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= bmnc14l20
         (465 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar...   201   5e-53
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch...   192   2e-50
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces...   105   4e-24
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|...    91   6e-20
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||...    26   3.3  
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr...    26   3.3  
SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces pom...    25   4.3  
SPBC13G1.05 |||DUF747 family protein|Schizosaccharomyces pombe|c...    25   7.5  
SPAC16C9.07 |ppk5|SPAC2G11.01, mug189|serine/threonine protein k...    25   7.5  

>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
           2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 449

 Score =  201 bits (490), Expect = 5e-53
 Identities = 87/121 (71%), Positives = 101/121 (83%)
 Frame = +2

Query: 101 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 280
           MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M  +      D  F+TFFSETG GK
Sbjct: 1   MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60

Query: 281 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPXQLITGKEDAANNYARGHYTIGKEIVDLVLD 460
           +VPR+++VDLEP V+D+VRTG YR LFHP QLITGKEDA+NNYARGHYT+GKE+VD V D
Sbjct: 61  YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120

Query: 461 R 463
           +
Sbjct: 121 K 121


>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 455

 Score =  192 bits (469), Expect = 2e-50
 Identities = 88/126 (69%), Positives = 103/126 (81%), Gaps = 5/126 (3%)
 Frame = +2

Query: 101 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSE 265
           MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG  PT+     K     +D F TFFSE
Sbjct: 1   MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59

Query: 266 TGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPXQLITGKEDAANNYARGHYTIGKEIV 445
           TG GK VPR+++VDLEP V+D+VRTG Y+ LFHP Q++TGKEDA+NNYARGHYT+GKE++
Sbjct: 60  TGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMI 119

Query: 446 DLVLDR 463
           D VL+R
Sbjct: 120 DSVLER 125


>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 448

 Score =  105 bits (251), Expect = 4e-24
 Identities = 51/120 (42%), Positives = 68/120 (56%)
 Frame = +2

Query: 101 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 280
           MRE + +  GQ G Q+G A W     EHG+   G      T     +  N +F+E   GK
Sbjct: 1   MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58

Query: 281 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPXQLITGKEDAANNYARGHYTIGKEIVDLVLD 460
           +VPRAV VDLEP  +D V++G +  LF P  +I G+  A N +A+GHYT G E+ D VLD
Sbjct: 59  YVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLD 118


>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 446

 Score = 91.5 bits (217), Expect = 6e-20
 Identities = 46/121 (38%), Positives = 73/121 (60%), Gaps = 2/121 (1%)
 Frame = +2

Query: 104 RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKH 283
           RE I++  GQ G QIG+  W+  CLEHGI PDG + +  T   G D  + FF ++   ++
Sbjct: 3   REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRY 60

Query: 284 VPRAVFVDLEPTVVDEVRTGTYRQLFHPXQLITGKE--DAANNYARGHYTIGKEIVDLVL 457
           +PRA+ +DLEP VV+ + + TY  L++P  ++  K    A NN+A G Y+  + I + ++
Sbjct: 61  IPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIM 119

Query: 458 D 460
           D
Sbjct: 120 D 120


>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 534

 Score = 25.8 bits (54), Expect = 3.3
 Identities = 15/35 (42%), Positives = 19/35 (54%)
 Frame = +3

Query: 180 STASSLMARCPQTRPSGVETILSTLSSARPELAST 284
           ST SSL +    ++PS   T  ST SSA P   S+
Sbjct: 173 STFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSS 207


>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 1275

 Score = 25.8 bits (54), Expect = 3.3
 Identities = 13/22 (59%), Positives = 13/22 (59%)
 Frame = +1

Query: 199 WPDAHRQDHRGWRRFFQHFLQR 264
           W  A R D R  R  FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611


>SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 534

 Score = 25.4 bits (53), Expect = 4.3
 Identities = 15/41 (36%), Positives = 21/41 (51%)
 Frame = +3

Query: 90  LKSKCVSAYLYTLAKPESRSVMPAGSFTAWSTASSLMARCP 212
           L+S        +L+ P SR++ P  S +  STASSL    P
Sbjct: 170 LRSSMPLVMANSLSPPSSRALKPIHSLSNPSTASSLEPSSP 210


>SPBC13G1.05 |||DUF747 family protein|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 649

 Score = 24.6 bits (51), Expect = 7.5
 Identities = 10/23 (43%), Positives = 14/23 (60%)
 Frame = +2

Query: 386 KEDAANNYARGHYTIGKEIVDLV 454
           +E  ANN+ RGH  I K+  D +
Sbjct: 599 RERKANNFFRGHIEIDKKTKDFL 621


>SPAC16C9.07 |ppk5|SPAC2G11.01, mug189|serine/threonine protein
           kinase Ppk5 |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 836

 Score = 24.6 bits (51), Expect = 7.5
 Identities = 12/22 (54%), Positives = 14/22 (63%)
 Frame = -3

Query: 127 NVYRYALTHFDLSFFLCLNVEL 62
           NV RY L+HFD    LC+  EL
Sbjct: 579 NVIRY-LSHFDFRGHLCIVTEL 599


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,979,387
Number of Sequences: 5004
Number of extensions: 39719
Number of successful extensions: 111
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 106
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 108
length of database: 2,362,478
effective HSP length: 67
effective length of database: 2,027,210
effective search space used: 176367270
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)