BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmnc14f23
(495 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBP26C9.03c |||iron ion transporter |Schizosaccharomyces pombe|... 28 0.67
SPAC4D7.01c |sec71|sec7a, SPAP8A3.15c|Sec7 domain|Schizosaccharo... 28 0.89
SPCC74.06 |mak3|phk2|histidine kinase Mak3 |Schizosaccharomyces ... 27 1.6
SPAC1142.04 |||Noc2p-Noc3p complex subunit Noc2 family |Schizosa... 27 1.6
SPBC29A10.04 |psm1|smc1|mitotic cohesin complex subunit Psm1 |Sc... 25 4.7
SPAC13G6.01c |rad8|SPAC5H10.14c|ubiquitin-protein ligase E3 |Sch... 25 4.7
SPCC4G3.12c |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 25 4.7
SPAC19A8.08 |upf2||nonsense-mediated decay protein Upf2|Schizosa... 25 6.3
SPAPB1E7.05 |gde1||glycerophosphoryl diester phosphodiesterase G... 25 6.3
SPAC1565.07c |||TATA binding protein interacting protein |Schizo... 25 6.3
SPCC4G3.08 |psk1||serine/threonine protein kinase Psk1|Schizosac... 25 6.3
SPAC3A12.05c |taf2||TATA-binding protein associated factor Taf2|... 25 6.3
SPBP8B7.30c |thi5||transcription factor Thi5|Schizosaccharomyces... 25 8.3
SPAC22A12.14c |||BSD domain protein, unknown biological role|Sch... 25 8.3
SPBC29A3.13 |||PWWP domain protein|Schizosaccharomyces pombe|chr... 25 8.3
>SPBP26C9.03c |||iron ion transporter |Schizosaccharomyces pombe|chr
2|||Manual
Length = 584
Score = 28.3 bits (60), Expect = 0.67
Identities = 13/50 (26%), Positives = 26/50 (52%)
Frame = +2
Query: 254 TVKLRMIWRSIWSIIGTSRRLCKHWRVILISGSD*RKLMKKMLNLEKSQN 403
T L +I +W+I+G R +W++++ GS + + L + + QN
Sbjct: 163 TFILMLIILIVWAIVGGIYRAPDNWQIVMQDGSSIQCYVSDTLLMRQQQN 212
>SPAC4D7.01c |sec71|sec7a, SPAP8A3.15c|Sec7 domain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1811
Score = 27.9 bits (59), Expect = 0.89
Identities = 9/15 (60%), Positives = 13/15 (86%)
Frame = +2
Query: 260 KLRMIWRSIWSIIGT 304
++RM W SIWS++GT
Sbjct: 1207 RIRMEWSSIWSLLGT 1221
>SPCC74.06 |mak3|phk2|histidine kinase Mak3 |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 2344
Score = 27.1 bits (57), Expect = 1.6
Identities = 15/36 (41%), Positives = 17/36 (47%), Gaps = 1/36 (2%)
Frame = +3
Query: 105 SNENCHYLHVDLVVLAKLTHQYEALS-YNRLYDRFP 209
S E CHYLH+ K+ EAL YNR P
Sbjct: 825 SVEYCHYLHLAAEEALKIGANQEALDLYNRCIKMIP 860
>SPAC1142.04 |||Noc2p-Noc3p complex subunit Noc2 family
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 707
Score = 27.1 bits (57), Expect = 1.6
Identities = 13/45 (28%), Positives = 24/45 (53%)
Frame = +1
Query: 241 DKSEDSEIKDDLEEYMEYHRYLKEVVQALESDPDFRERLEKADEE 375
+ ++DS + EE Y K++ E DP+F + LE+ D++
Sbjct: 102 ENTQDSTSESSEEEEDGLESYQKQLEGLKEKDPEFYKFLEQNDQD 146
>SPBC29A10.04 |psm1|smc1|mitotic cohesin complex subunit Psm1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1233
Score = 25.4 bits (53), Expect = 4.7
Identities = 16/53 (30%), Positives = 26/53 (49%)
Frame = +1
Query: 244 KSEDSEIKDDLEEYMEYHRYLKEVVQALESDPDFRERLEKADEEDVKSGKIAE 402
+S S +++ L EY E + ++A+E RL K DEE + K A+
Sbjct: 1001 ESMASVLQEKLREYSEELDQMSPNLRAIERLETVETRLAKLDEEFAAARKAAK 1053
>SPAC13G6.01c |rad8|SPAC5H10.14c|ubiquitin-protein ligase E3
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1133
Score = 25.4 bits (53), Expect = 4.7
Identities = 15/47 (31%), Positives = 23/47 (48%), Gaps = 4/47 (8%)
Frame = +2
Query: 323 HW-RVILISGSD*RKLMKKMLNL---EKSQNSWTLSTTNVRTRLDEI 451
HW RV+L G + R K SQN W ++ T + +LD++
Sbjct: 649 HWFRVVLDEGHNIRNRESKTAKACHSISSQNRWVITGTPIVNKLDDL 695
>SPCC4G3.12c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 821
Score = 25.4 bits (53), Expect = 4.7
Identities = 10/22 (45%), Positives = 15/22 (68%)
Frame = +3
Query: 51 YIITGQQNSTYSKIIQSISNEN 116
Y + NSTY+ +QSI+NE+
Sbjct: 184 YSLPSNSNSTYTTPLQSINNEH 205
>SPAC19A8.08 |upf2||nonsense-mediated decay protein
Upf2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1049
Score = 25.0 bits (52), Expect = 6.3
Identities = 10/19 (52%), Positives = 14/19 (73%)
Frame = -1
Query: 132 HVSNDSFRLILIVLFLNRC 76
+++ND FRL LIV+ L C
Sbjct: 789 NINNDMFRLRLIVVVLQTC 807
>SPAPB1E7.05 |gde1||glycerophosphoryl diester phosphodiesterase
Gde1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1076
Score = 25.0 bits (52), Expect = 6.3
Identities = 10/28 (35%), Positives = 18/28 (64%)
Frame = +1
Query: 253 DSEIKDDLEEYMEYHRYLKEVVQALESD 336
DSE+KD LE+ +H K+++ ++ D
Sbjct: 126 DSEVKDKLEKLSIHHPLKKDLLAFIQLD 153
>SPAC1565.07c |||TATA binding protein interacting protein
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1220
Score = 25.0 bits (52), Expect = 6.3
Identities = 10/26 (38%), Positives = 16/26 (61%)
Frame = +1
Query: 217 SYAPPVTPDKSEDSEIKDDLEEYMEY 294
SY P + D +++ +I D LEE +Y
Sbjct: 313 SYDPNLISDTNDEDDIADFLEEMSDY 338
>SPCC4G3.08 |psk1||serine/threonine protein kinase
Psk1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 436
Score = 25.0 bits (52), Expect = 6.3
Identities = 16/65 (24%), Positives = 30/65 (46%)
Frame = +1
Query: 247 SEDSEIKDDLEEYMEYHRYLKEVVQALESDPDFRERLEKADEEDVKSGKIAEQLDFVNHK 426
S D EI ++ ++ E L+ + S D ++ DE+ K+A DF++ K
Sbjct: 21 SSDDEIAEEGYDFEE----LEASASTITSSSDLKDGKNAKDEKGTVEFKVAAHGDFISSK 76
Query: 427 CEDKV 441
++ V
Sbjct: 77 GDNYV 81
>SPAC3A12.05c |taf2||TATA-binding protein associated factor
Taf2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1174
Score = 25.0 bits (52), Expect = 6.3
Identities = 16/59 (27%), Positives = 26/59 (44%), Gaps = 2/59 (3%)
Frame = +2
Query: 275 WRSIWSIIGTSRRLCKHWRVILISGSD*RKLMKKMLN--LEKSQNSWTLSTTNVRTRLD 445
W +W I G S +C + L+ +D R +KK + LE +S N+ +D
Sbjct: 403 WSDLWLIYGLSYYICGLFLKKLMGNNDYRFRLKKQVYRLLELDIGKPPISQRNINIPID 461
>SPBP8B7.30c |thi5||transcription factor Thi5|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 857
Score = 24.6 bits (51), Expect = 8.3
Identities = 11/20 (55%), Positives = 13/20 (65%)
Frame = +2
Query: 311 RLCKHWRVILISGSD*RKLM 370
+LCKH L SGSD R L+
Sbjct: 110 KLCKHVESSLASGSDVRSLL 129
>SPAC22A12.14c |||BSD domain protein, unknown biological
role|Schizosaccharomyces pombe|chr 1|||Manual
Length = 347
Score = 24.6 bits (51), Expect = 8.3
Identities = 11/50 (22%), Positives = 24/50 (48%)
Frame = +1
Query: 229 PVTPDKSEDSEIKDDLEEYMEYHRYLKEVVQALESDPDFRERLEKADEED 378
P + D D E+K++ E + ++ +LE + +++ DE+D
Sbjct: 294 PASHDGEVDGEVKEEEENKVSSSSNIEASQSSLEVKDEANRKVDDDDEDD 343
>SPBC29A3.13 |||PWWP domain protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 359
Score = 24.6 bits (51), Expect = 8.3
Identities = 16/51 (31%), Positives = 26/51 (50%)
Frame = +1
Query: 253 DSEIKDDLEEYMEYHRYLKEVVQALESDPDFRERLEKADEEDVKSGKIAEQ 405
DSEI L + L E +A ++ PD +E E + +E++ S AE+
Sbjct: 113 DSEIAKFLGSSKRKSKELIEAYEASKTPPDLKE--ESSTDEEMDSLSAAEE 161
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,776,331
Number of Sequences: 5004
Number of extensions: 32468
Number of successful extensions: 149
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 144
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 149
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 194131776
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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