Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmnc14d11
(447 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC1604.21c |ptr3|uba1, SPBC211.09|ubiquitin activating enzyme ... 101 7e-23
SPBC16H5.03c |fub2|uba2|SUMO E1-like activator enzyme Fub2|Schiz... 46 4e-06
SPAC4C5.04 |rad31|uba4|SUMO E1-like activator enzyme Rad31|Schiz... 44 1e-05
SPAC1A6.10 ||SPAC30D11.15c|Moeb/ThiF domain|Schizosaccharomyces ... 35 0.007
SPBC6B1.05c |||ubiquitin-like conjugating enzyme|Schizosaccharom... 30 0.14
SPAC323.06c |uba5||NEDD8 activating enzyme |Schizosaccharomyces ... 28 0.57
SPAC23D3.13c |||guanyl-nucleotide exchange factor|Schizosaccharo... 27 0.99
SPAC212.11 |tlh1||RecQ type DNA helicase|Schizosaccharomyces pom... 27 1.7
SPBCPT2R1.08c |tlh2||RecQ type DNA helicase Tlh1|Schizosaccharom... 27 1.7
SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 27 1.7
SPBC4B4.04 |||translation initiation factor eIF2A |Schizosacchar... 25 5.3
SPBC1711.04 |||methylenetetrahydrofolate reductase |Schizosaccha... 25 7.0
SPAP8A3.13c |||Vid 24 family protein|Schizosaccharomyces pombe|c... 25 7.0
SPCP31B10.05 |||tyrosyl-DNA phosphodiesterase |Schizosaccharomyc... 25 7.0
SPAC23C11.04c |pnk1||DNA kinase/phosphatase Pnk1|Schizosaccharom... 25 7.0
SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces pombe... 24 9.3
SPAC23G3.08c |ubp7||ubiquitin C-terminal hydrolase Ubp7|Schizosa... 24 9.3
>SPBC1604.21c |ptr3|uba1, SPBC211.09|ubiquitin activating enzyme
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1012
Score = 101 bits (241), Expect = 7e-23
Identities = 48/74 (64%), Positives = 61/74 (82%)
Frame = +1
Query: 223 EDEIDESLYSRQLYVLGHDAMRRMASSDVLISGLGGLGVEIAKNVILGGVKSVTLHDDKN 402
++ IDE LYSRQLYVLGH+AM++M+ S+VLI G GLGVEIAKNV L GVKSVTL+D +
Sbjct: 12 QNTIDEGLYSRQLYVLGHEAMKQMSQSNVLIIGCKGLGVEIAKNVCLAGVKSVTLYDPQP 71
Query: 403 CTVADLSSQFYLSE 444
+ DLSSQ++L+E
Sbjct: 72 TRIEDLSSQYFLTE 85
>SPBC16H5.03c |fub2|uba2|SUMO E1-like activator enzyme
Fub2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 628
Score = 45.6 bits (103), Expect = 4e-06
Identities = 20/52 (38%), Positives = 34/52 (65%)
Frame = +1
Query: 277 DAMRRMASSDVLISGLGGLGVEIAKNVILGGVKSVTLHDDKNCTVADLSSQF 432
+A+R S+ VL+ G GG+G E+ KN+++ GVK V + D +++L+ QF
Sbjct: 18 EALRNFKSAKVLLVGAGGIGCELLKNLLMSGVKEVHIIDLDTIDLSNLNRQF 69
>SPAC4C5.04 |rad31|uba4|SUMO E1-like activator enzyme
Rad31|Schizosaccharomyces pombe|chr 1|||Manual
Length = 307
Score = 43.6 bits (98), Expect = 1e-05
Identities = 26/80 (32%), Positives = 42/80 (52%)
Frame = +1
Query: 199 MANNGARVEDEIDESLYSRQLYVLGHDAMRRMASSDVLISGLGGLGVEIAKNVILGGVKS 378
M N+ E EI +LY RQ+ + G +A + + S VL+ L EIAKN++L G+
Sbjct: 1 MGNHNINAE-EI--ALYDRQIRLWGFNAQQALKQSRVLLITASPLANEIAKNLVLSGIGK 57
Query: 379 VTLHDDKNCTVADLSSQFYL 438
+ + D D+ QF++
Sbjct: 58 LCVLDSMTVYEKDVEEQFFI 77
>SPAC1A6.10 ||SPAC30D11.15c|Moeb/ThiF domain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 485
Score = 34.7 bits (76), Expect = 0.007
Identities = 16/73 (21%), Positives = 35/73 (47%)
Frame = +1
Query: 205 NNGARVEDEIDESLYSRQLYVLGHDAMRRMASSDVLISGLGGLGVEIAKNVILGGVKSVT 384
+ G ++ + +R G D M R+ +S V++ G GG+G + + GV+ +
Sbjct: 95 SKGVPYDENLIREQLARNYAFFGEDGMERLRNSFVIVVGCGGVGSWVINMLARSGVQKIR 154
Query: 385 LHDDKNCTVADLS 423
+ D +++ L+
Sbjct: 155 IVDFDQVSLSSLN 167
>SPBC6B1.05c |||ubiquitin-like conjugating
enzyme|Schizosaccharomyces pombe|chr 2|||Manual
Length = 649
Score = 30.3 bits (65), Expect = 0.14
Identities = 14/35 (40%), Positives = 22/35 (62%)
Frame = +1
Query: 289 RMASSDVLISGLGGLGVEIAKNVILGGVKSVTLHD 393
R+ +S L+ G G LG +A+N++ GV+ VT D
Sbjct: 332 RIQNSKCLLLGAGTLGCGVARNLLSWGVRHVTFVD 366
>SPAC323.06c |uba5||NEDD8 activating enzyme |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 500
Score = 28.3 bits (60), Expect = 0.57
Identities = 16/64 (25%), Positives = 29/64 (45%)
Frame = +1
Query: 247 YSRQLYVLGHDAMRRMASSDVLISGLGGLGVEIAKNVILGGVKSVTLHDDKNCTVADLSS 426
Y RQ+ + + + S V + +G E KN+IL G+ S + DD + +
Sbjct: 10 YDRQVRLWKAEGQNAIEKSHVCLLYANTVGCEALKNLILPGIGSFAVVDDTSVDFSMDGM 69
Query: 427 QFYL 438
F++
Sbjct: 70 NFFI 73
>SPAC23D3.13c |||guanyl-nucleotide exchange
factor|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1616
Score = 27.5 bits (58), Expect = 0.99
Identities = 10/30 (33%), Positives = 19/30 (63%)
Frame = +3
Query: 21 CCFKQFEENFFLIVNATKILAKLCAFVPDV 110
CC K+ E + ++ +N+ ++LA A PD+
Sbjct: 64 CCAKKIERHIYISLNSIQLLAINDALSPDI 93
>SPAC212.11 |tlh1||RecQ type DNA helicase|Schizosaccharomyces
pombe|chr 1||Partial|Manual
Length = 1887
Score = 26.6 bits (56), Expect = 1.7
Identities = 31/109 (28%), Positives = 49/109 (44%), Gaps = 7/109 (6%)
Frame = +1
Query: 85 NCARSCPMSSAEVAD-----NSVDPPAKKR--KLNTGEAHSNNSAMANNGARVEDEIDES 243
N + P+S +E+++ +SV P KK NT + ++ + DE
Sbjct: 1637 NTTSTTPVSLSELSEITLFPSSVSPTWKKSFGNANTNLKYGLEDMSLSHRRGHKRTYDEH 1696
Query: 244 LYSRQLYVLGHDAMRRMASSDVLISGLGGLGVEIAKNVILGGVKSVTLH 390
L + Q V HD M R+ S +SG+ G+G+ I G V S T+H
Sbjct: 1697 LNNVQQGV-NHD-MNRVHGSVGGMSGIVGIGIGIGDGDGDGDVDSRTIH 1743
>SPBCPT2R1.08c |tlh2||RecQ type DNA helicase Tlh1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1919
Score = 26.6 bits (56), Expect = 1.7
Identities = 31/109 (28%), Positives = 49/109 (44%), Gaps = 7/109 (6%)
Frame = +1
Query: 85 NCARSCPMSSAEVAD-----NSVDPPAKKR--KLNTGEAHSNNSAMANNGARVEDEIDES 243
N + P+S +E+++ +SV P KK NT + ++ + DE
Sbjct: 1637 NTTSTTPVSLSELSEITLFPSSVSPTWKKSFGNANTNLKYGLEDMSLSHRRGHKRTYDEH 1696
Query: 244 LYSRQLYVLGHDAMRRMASSDVLISGLGGLGVEIAKNVILGGVKSVTLH 390
L + Q V HD M R+ S +SG+ G+G+ I G V S T+H
Sbjct: 1697 LNNVQQGV-NHD-MNRVHGSVGGMSGIVGIGIGIGDGDGDGDVDSRTIH 1743
>SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit
Arp9|Schizosaccharomyces pombe|chr 1|||Manual
Length = 523
Score = 26.6 bits (56), Expect = 1.7
Identities = 11/20 (55%), Positives = 13/20 (65%)
Frame = -2
Query: 425 EERSATVQFLSSCKVTDFTP 366
EE++ F S CKV DFTP
Sbjct: 87 EEKNMGSSFHSECKVDDFTP 106
>SPBC4B4.04 |||translation initiation factor eIF2A
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 576
Score = 25.0 bits (52), Expect = 5.3
Identities = 15/47 (31%), Positives = 23/47 (48%), Gaps = 2/47 (4%)
Frame = +1
Query: 307 VLISGLGGLGVEIAKNVILGGVKSVTLHDDKNCTVADLS--SQFYLS 441
+L++G G L I +K +T + NCT + S SQF L+
Sbjct: 324 ILLAGFGNLQGSIDIFDAANNMKKITTVEAANCTYCEFSPDSQFLLT 370
>SPBC1711.04 |||methylenetetrahydrofolate reductase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 320
Score = 24.6 bits (51), Expect = 7.0
Identities = 11/40 (27%), Positives = 18/40 (45%)
Frame = +1
Query: 301 SDVLISGLGGLGVEIAKNVILGGVKSVTLHDDKNCTVADL 420
SDV+I G+ + N++ G + +KN A L
Sbjct: 244 SDVIICGVPSANYKFPSNLVRDGAVCICFSSEKNFDAASL 283
>SPAP8A3.13c |||Vid 24 family protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 547
Score = 24.6 bits (51), Expect = 7.0
Identities = 19/66 (28%), Positives = 29/66 (43%)
Frame = -2
Query: 332 KPPRPDIRTSELAIRRIASWPST*SWREYRLSSISSSTRAPLFAIAELFECASPVFSFRF 153
+PP P R+ ++ + S+PS+ Y + S+ PL I L SP+
Sbjct: 201 QPPLPPSRSISISNPQSLSFPSSFDQSNYNFQAASTPQFNPL--IEHLRRSNSPLNPSHD 258
Query: 152 FAGGST 135
AG ST
Sbjct: 259 SAGAST 264
>SPCP31B10.05 |||tyrosyl-DNA phosphodiesterase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 536
Score = 24.6 bits (51), Expect = 7.0
Identities = 8/19 (42%), Positives = 13/19 (68%)
Frame = +1
Query: 376 SVTLHDDKNCTVADLSSQF 432
S+ + DD+NC + D+ S F
Sbjct: 190 SLDIKDDRNCDIIDVDSAF 208
>SPAC23C11.04c |pnk1||DNA kinase/phosphatase
Pnk1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 421
Score = 24.6 bits (51), Expect = 7.0
Identities = 9/23 (39%), Positives = 15/23 (65%)
Frame = +2
Query: 254 ASSTCWATTLCVEWRVLMSLYLA 322
+SSTC + ++W + SLY+A
Sbjct: 37 SSSTCLQQKIEIQWSITDSLYIA 59
>SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 848
Score = 24.2 bits (50), Expect = 9.3
Identities = 9/42 (21%), Positives = 20/42 (47%)
Frame = +1
Query: 118 EVADNSVDPPAKKRKLNTGEAHSNNSAMANNGARVEDEIDES 243
E N ++P +T E + +++ + ++ EID+S
Sbjct: 804 ETFTNDIEPTTALNSTSTQETEKSRASVTQRPSSLQQEIDDS 845
>SPAC23G3.08c |ubp7||ubiquitin C-terminal hydrolase
Ubp7|Schizosaccharomyces pombe|chr 1|||Manual
Length = 875
Score = 24.2 bits (50), Expect = 9.3
Identities = 18/76 (23%), Positives = 30/76 (39%)
Frame = +1
Query: 34 NLKKISS*LSTQPKFWQNCARSCPMSSAEVADNSVDPPAKKRKLNTGEAHSNNSAMANNG 213
NL +S L P + S ++S + N D NT + NN +
Sbjct: 326 NLNSVSRSLQLSPCLTDDEQLSKSLTSFKQV-NVTDASLSPNSHNTSDNEQNNEDYVSVS 384
Query: 214 ARVEDEIDESLYSRQL 261
+ V E ++ YS++L
Sbjct: 385 SLVGSETEDITYSKEL 400
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,902,026
Number of Sequences: 5004
Number of extensions: 37615
Number of successful extensions: 137
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 135
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 137
length of database: 2,362,478
effective HSP length: 67
effective length of database: 2,027,210
effective search space used: 164204010
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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