Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmnc13b10
(711 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC1805.12c |uep1|ubi2|ribosomal-ubiquitin fusion protein Ubi2|... 66 5e-12
SPAC11G7.04 |ubi1||ribosomal-ubiquitin fusion protein Ubi1|Schiz... 66 5e-12
SPAC6G10.11c |ubi3||ribosomal ubiquitin fusion protein Ubi3|Schi... 66 5e-12
SPBC337.08c |ubi4||ubiquitin|Schizosaccharomyces pombe|chr 2|||M... 66 5e-12
SPAC589.10c |||ribomal-ubiquitin fusion protein Ubi5|Schizosacch... 66 5e-12
SPBC12D12.08c |ned8|nedd8, ubl1, SPBC24C6.01c|ubiquitin-like pro... 48 2e-06
SPBC1703.10 |ypt1||GTPase Ypt1|Schizosaccharomyces pombe|chr 2||... 33 0.053
SPAC22A12.10 |||diacylglycerol cholinephosphotranferase/ diacylg... 27 2.0
SPAC9E9.07c |ypt2||GTPase Ypt2 |Schizosaccharomyces pombe|chr 1|... 27 2.0
SPBC29A10.04 |psm1|smc1|mitotic cohesin complex subunit Psm1 |Sc... 27 3.5
SPBC16G5.03 |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 26 4.6
SPBC409.05 |skp1|psh1, sph1|SCF ubiquitin ligase complex subunit... 26 6.1
SPAPB1A10.07c |||sphingolipid biosynthesis protein|Schizosacchar... 26 6.1
SPAC1D4.14 |tho2|SPAC22F3.14c|THO complex subunit Tho2 |Schizosa... 25 8.1
>SPAC1805.12c |uep1|ubi2|ribosomal-ubiquitin fusion protein
Ubi2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 128
Score = 66.1 bits (154), Expect = 5e-12
Identities = 29/43 (67%), Positives = 36/43 (83%)
Frame = -3
Query: 130 MQIFIKTLTGKTITAETEPAETVADLKQKIADKEGVPVDQQRL 2
MQIF+KTLTGKTIT E E ++T+ ++K KI DKEG+P DQQRL
Sbjct: 1 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRL 43
>SPAC11G7.04 |ubi1||ribosomal-ubiquitin fusion protein
Ubi1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 128
Score = 66.1 bits (154), Expect = 5e-12
Identities = 29/43 (67%), Positives = 36/43 (83%)
Frame = -3
Query: 130 MQIFIKTLTGKTITAETEPAETVADLKQKIADKEGVPVDQQRL 2
MQIF+KTLTGKTIT E E ++T+ ++K KI DKEG+P DQQRL
Sbjct: 1 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRL 43
>SPAC6G10.11c |ubi3||ribosomal ubiquitin fusion protein
Ubi3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 150
Score = 66.1 bits (154), Expect = 5e-12
Identities = 29/43 (67%), Positives = 36/43 (83%)
Frame = -3
Query: 130 MQIFIKTLTGKTITAETEPAETVADLKQKIADKEGVPVDQQRL 2
MQIF+KTLTGKTIT E E ++T+ ++K KI DKEG+P DQQRL
Sbjct: 1 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRL 43
>SPBC337.08c |ubi4||ubiquitin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 382
Score = 66.1 bits (154), Expect = 5e-12
Identities = 29/43 (67%), Positives = 36/43 (83%)
Frame = -3
Query: 130 MQIFIKTLTGKTITAETEPAETVADLKQKIADKEGVPVDQQRL 2
MQIF+KTLTGKTIT E E ++T+ ++K KI DKEG+P DQQRL
Sbjct: 1 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRL 43
Score = 66.1 bits (154), Expect = 5e-12
Identities = 29/43 (67%), Positives = 36/43 (83%)
Frame = -3
Query: 130 MQIFIKTLTGKTITAETEPAETVADLKQKIADKEGVPVDQQRL 2
MQIF+KTLTGKTIT E E ++T+ ++K KI DKEG+P DQQRL
Sbjct: 77 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRL 119
Score = 66.1 bits (154), Expect = 5e-12
Identities = 29/43 (67%), Positives = 36/43 (83%)
Frame = -3
Query: 130 MQIFIKTLTGKTITAETEPAETVADLKQKIADKEGVPVDQQRL 2
MQIF+KTLTGKTIT E E ++T+ ++K KI DKEG+P DQQRL
Sbjct: 153 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRL 195
Score = 66.1 bits (154), Expect = 5e-12
Identities = 29/43 (67%), Positives = 36/43 (83%)
Frame = -3
Query: 130 MQIFIKTLTGKTITAETEPAETVADLKQKIADKEGVPVDQQRL 2
MQIF+KTLTGKTIT E E ++T+ ++K KI DKEG+P DQQRL
Sbjct: 229 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRL 271
Score = 66.1 bits (154), Expect = 5e-12
Identities = 29/43 (67%), Positives = 36/43 (83%)
Frame = -3
Query: 130 MQIFIKTLTGKTITAETEPAETVADLKQKIADKEGVPVDQQRL 2
MQIF+KTLTGKTIT E E ++T+ ++K KI DKEG+P DQQRL
Sbjct: 305 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRL 347
>SPAC589.10c |||ribomal-ubiquitin fusion protein
Ubi5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 150
Score = 66.1 bits (154), Expect = 5e-12
Identities = 29/43 (67%), Positives = 36/43 (83%)
Frame = -3
Query: 130 MQIFIKTLTGKTITAETEPAETVADLKQKIADKEGVPVDQQRL 2
MQIF+KTLTGKTIT E E ++T+ ++K KI DKEG+P DQQRL
Sbjct: 1 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRL 43
>SPBC12D12.08c |ned8|nedd8, ubl1, SPBC24C6.01c|ubiquitin-like
protein modifier Ned8|Schizosaccharomyces pombe|chr
2|||Manual
Length = 78
Score = 47.6 bits (108), Expect = 2e-06
Identities = 20/43 (46%), Positives = 31/43 (72%)
Frame = -3
Query: 130 MQIFIKTLTGKTITAETEPAETVADLKQKIADKEGVPVDQQRL 2
M I +KTLTGK I + +P + V+ +K+++ +KEG+P QQRL
Sbjct: 1 MLIKVKTLTGKEIELDIDPNDKVSRIKERVEEKEGIPPSQQRL 43
>SPBC1703.10 |ypt1||GTPase Ypt1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 203
Score = 32.7 bits (71), Expect = 0.053
Identities = 18/57 (31%), Positives = 31/57 (54%)
Frame = +3
Query: 129 IFTLLLINDNGCCECALASTDGGIVQQAADTHTTNYFPSTAIDFHARNQKLSGRRAR 299
+F LLLI D+G + L +++ A DT+T +Y + +DF R +L G+ +
Sbjct: 8 LFKLLLIGDSGVGKSCL------LLRFADDTYTESYISTIGVDFKIRTFELEGKTVK 58
>SPAC22A12.10 |||diacylglycerol cholinephosphotranferase/
diacylglycerol ethanolaminesphotranferase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 386
Score = 27.5 bits (58), Expect = 2.0
Identities = 17/62 (27%), Positives = 30/62 (48%)
Frame = -1
Query: 450 FAAEIARLIHFKIVVSFYNFWQQSALLPHCIDHLAQVARCYIKSFESIYRFLHVGLIIFD 271
FA + +I I S + +W +LP +D + +K+ ++ Y F +VG+ I
Sbjct: 296 FAYSVGVVIVSHITESPFPYWNV-LILPFLVDAVDAYTFGVLKNVQTEYFFCYVGICIGV 354
Query: 270 YG 265
YG
Sbjct: 355 YG 356
>SPAC9E9.07c |ypt2||GTPase Ypt2 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 200
Score = 27.5 bits (58), Expect = 2.0
Identities = 16/57 (28%), Positives = 30/57 (52%)
Frame = +3
Query: 129 IFTLLLINDNGCCECALASTDGGIVQQAADTHTTNYFPSTAIDFHARNQKLSGRRAR 299
+ LLLI D+G + L +++ + D+ T ++ + IDF R +L G+R +
Sbjct: 9 LIKLLLIGDSGVGKSCL------LLRFSEDSFTPSFITTIGIDFKIRTIELDGKRIK 59
>SPBC29A10.04 |psm1|smc1|mitotic cohesin complex subunit Psm1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1233
Score = 26.6 bits (56), Expect = 3.5
Identities = 17/66 (25%), Positives = 35/66 (53%)
Frame = -3
Query: 202 TIPPSVEARAHSQQPLSFISNSVKMQIFIKTLTGKTITAETEPAETVADLKQKIADKEGV 23
T+ +++ + S+ L+ I ++ +I K+L + +TE A+ +A + +KI E
Sbjct: 403 TLNRNIKVTSQSKDSLTSIVGDLESKI--KSLHESVSSLDTERADLLAKINEKIESLELE 460
Query: 22 PVDQQR 5
DQQ+
Sbjct: 461 KHDQQK 466
>SPBC16G5.03 |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 268
Score = 26.2 bits (55), Expect = 4.6
Identities = 11/39 (28%), Positives = 19/39 (48%), Gaps = 2/39 (5%)
Frame = +1
Query: 481 DKLFRCRLCYTHADWLWCDFHRNHAYRG--TRDIACNNY 591
D+ C +C H D ++ FH + Y+ T+ + N Y
Sbjct: 52 DQSLTCPICKRHVDCVFYGFHGSSLYKKWYTQSLGSNQY 90
>SPBC409.05 |skp1|psh1, sph1|SCF ubiquitin ligase complex subunit
Skp1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 161
Score = 25.8 bits (54), Expect = 6.1
Identities = 7/15 (46%), Positives = 10/15 (66%)
Frame = +1
Query: 529 WCDFHRNHAYRGTRD 573
WC+ H+N Y GT +
Sbjct: 57 WCEHHKNDLYSGTEE 71
>SPAPB1A10.07c |||sphingolipid biosynthesis
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 441
Score = 25.8 bits (54), Expect = 6.1
Identities = 10/22 (45%), Positives = 16/22 (72%), Gaps = 1/22 (4%)
Frame = +1
Query: 613 MGVVMLVEEY-FYCLSSCNFKQ 675
+G+V+ + Y F+C SSC+F Q
Sbjct: 212 VGLVLTILTYVFFCASSCSFNQ 233
Score = 25.4 bits (53), Expect = 8.1
Identities = 27/88 (30%), Positives = 36/88 (40%), Gaps = 7/88 (7%)
Frame = -3
Query: 451 FCS*DCAFDSL*NRRQLL*FLATVCL-VAPLHRSPRP------SGPVLY*VV*KHLSLLA 293
FC+ C+F+ N LL +A CL V P + P S V+ LS LA
Sbjct: 224 FCASSCSFNQAINTINLLLCIAVSCLSVHPTIQEYNPRSGLAQSSMVMCYTCYLILSALA 283
Query: 292 RRPDNF*LRAWKSIAVEGK*FVVWVSAA 209
RPD W + A + F + AA
Sbjct: 284 NRPDEGQCNPWGNSASGTREFSKVIGAA 311
>SPAC1D4.14 |tho2|SPAC22F3.14c|THO complex subunit Tho2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1628
Score = 25.4 bits (53), Expect = 8.1
Identities = 11/31 (35%), Positives = 17/31 (54%)
Frame = -2
Query: 134 KNANIHQNIDGQNHYRRNGTRRDGGRSQAKN 42
K+ N Q+I G+++ R+N GRS N
Sbjct: 1518 KDKNKRQDISGESNSRQNNAISRAGRSNGSN 1548
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,074,741
Number of Sequences: 5004
Number of extensions: 66137
Number of successful extensions: 204
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 193
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 204
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 331187010
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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