BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmnc12k15
(639 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC20H4.06c |||RNA-binding protein|Schizosaccharomyces pombe|ch... 86 4e-18
SPAC1851.04c ||SPAC27D7.01c|guanyl-nucleotide exchange factor |S... 29 0.57
SPCC1450.12 |||conserved fungal protein|Schizosaccharomyces pomb... 28 1.3
SPAC2G11.04 |||RNA-binding protein, G-patch type |Schizosaccharo... 27 1.7
SPBC8D2.12c |||mitochondrial DNA binding protein |Schizosaccharo... 27 1.7
SPBC16D10.10 |||tRNA specific adenosine deaminase subunit Tad2 |... 27 3.0
SPAC6B12.03c |||HbrB family protein|Schizosaccharomyces pombe|ch... 26 4.0
SPAC631.01c |acp2||F-actin capping protein beta subunit |Schizos... 25 7.0
SPBC30B4.02c |||R3H and G-patch domain, unknown biological role|... 25 7.0
>SPAC20H4.06c |||RNA-binding protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 534
Score = 86.2 bits (204), Expect = 4e-18
Identities = 44/103 (42%), Positives = 60/103 (58%), Gaps = 2/103 (1%)
Frame = +2
Query: 131 TNSDSEDENFIRYGTPLEPYEEDEIPSKRKYQQQP--DQYAVDDHGRRRFHGAFTGGFSA 304
+N D ++ YG+P + + R Q P Q A D+ R+RFHGAFTGGFSA
Sbjct: 9 SNVDVHRHPYVVYGSPFD------LEPSRINQGVPVWKQEARDERNRKRFHGAFTGGFSA 62
Query: 305 GYMNSVGTPEGWTPTAFKSSRVDKAQISSQRPEDFMDEEDRGE 433
GY N+VG+ EGW P ++KSSR + + +D MDEEDR +
Sbjct: 63 GYFNTVGSKEGWQPKSWKSSRNENKSVHGMTIDDIMDEEDRAD 105
Score = 26.6 bits (56), Expect = 3.0
Identities = 15/42 (35%), Positives = 22/42 (52%), Gaps = 3/42 (7%)
Frame = +2
Query: 329 PEGWTPTAFKSSR---VDKAQISSQRPEDFMDEEDRGEFGIA 445
PEGW P F +S+ KAQ S++R F ++ + G A
Sbjct: 297 PEGWEPKLFTNSQGFSQKKAQTSNERLPLFAEKSETNSNGEA 338
>SPAC1851.04c ||SPAC27D7.01c|guanyl-nucleotide exchange factor
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1052
Score = 29.1 bits (62), Expect = 0.57
Identities = 8/32 (25%), Positives = 23/32 (71%)
Frame = -1
Query: 162 MKFSSSESELVIFEITYMFKTILELKTYKFII 67
+ ++SS+ + +++E++ +FK + E T+K ++
Sbjct: 899 LTYNSSQRDKLLYEVSLLFKDLQEFTTFKIVL 930
>SPCC1450.12 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 821
Score = 27.9 bits (59), Expect = 1.3
Identities = 14/45 (31%), Positives = 22/45 (48%)
Frame = +2
Query: 83 VFNSKIVLNIYVISNMTNSDSEDENFIRYGTPLEPYEEDEIPSKR 217
+F+ I + +S TN D F+ G + PYE+ +IP R
Sbjct: 21 LFSLLIADELNFVSEPTNLDHLGSPFVEKGKSVIPYEKSQIPVLR 65
>SPAC2G11.04 |||RNA-binding protein, G-patch type
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 301
Score = 27.5 bits (58), Expect = 1.7
Identities = 10/21 (47%), Positives = 14/21 (66%)
Frame = +2
Query: 566 ETAAVRMLCAMGWRPGQGTGE 628
E A++ML GW+ GQG G+
Sbjct: 253 ENVALKMLQRCGWKEGQGLGQ 273
>SPBC8D2.12c |||mitochondrial DNA binding protein
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 293
Score = 27.5 bits (58), Expect = 1.7
Identities = 9/14 (64%), Positives = 13/14 (92%)
Frame = +2
Query: 395 RPEDFMDEEDRGEF 436
RP++++DEED GEF
Sbjct: 199 RPKEYIDEEDEGEF 212
>SPBC16D10.10 |||tRNA specific adenosine deaminase subunit Tad2
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 367
Score = 26.6 bits (56), Expect = 3.0
Identities = 12/41 (29%), Positives = 21/41 (51%)
Frame = +3
Query: 147 KTKTSSVTELH*SHMKKMKFQAKENISNNQISMPLMTMVEG 269
KT +S+ + H MK + + +MPL+TM++G
Sbjct: 201 KTSSSNTVKYDTQHETYMKLAHEMPLGKPYFNMPLLTMIKG 241
>SPAC6B12.03c |||HbrB family protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 302
Score = 26.2 bits (55), Expect = 4.0
Identities = 13/32 (40%), Positives = 20/32 (62%), Gaps = 1/32 (3%)
Frame = +3
Query: 375 KHKYLHKDQRTLWMKK-IEGSLVLLLVRCRCA 467
+H+Y +D++ W KK I L L++RC CA
Sbjct: 113 QHRYTEQDKKN-WKKKVIIEDLNFLVLRCICA 143
>SPAC631.01c |acp2||F-actin capping protein beta subunit
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 268
Score = 25.4 bits (53), Expect = 7.0
Identities = 17/42 (40%), Positives = 22/42 (52%)
Frame = -2
Query: 614 DLVSSPWHRAYGPLLSRGLPVLTAAVLVHQVLFLHETVRVWI 489
D SPW Y P L GL V T V +V L+E +RV++
Sbjct: 67 DSYRSPWSNKYDPPLEDGL-VSTDRVRKLEV-SLNEAIRVYL 106
>SPBC30B4.02c |||R3H and G-patch domain, unknown biological
role|Schizosaccharomyces pombe|chr 2|||Manual
Length = 695
Score = 25.4 bits (53), Expect = 7.0
Identities = 15/54 (27%), Positives = 25/54 (46%)
Frame = +2
Query: 464 RSNFSGQKRSRPSQFHEGTIPGEPVLQQLVQAVHETAAVRMLCAMGWRPGQGTG 625
R S + S+ ++ ++G I GE + E R+L +GW G+G G
Sbjct: 623 RMTVSSVRSSKATRVYDGQIVGEDA----PEISKENPGRRLLEKLGWYAGKGLG 672
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,868,665
Number of Sequences: 5004
Number of extensions: 63451
Number of successful extensions: 201
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 196
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 201
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 285732116
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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