Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= bmnc12e19
         (726 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC1442.16c |zta1|SPCC285.01c|NADPH quinone oxidoreductase/ARE-...    68   2e-12
SPBC1773.06c |||alcohol dehydrogenase |Schizosaccharomyces pombe...    62   1e-10
SPCC13B11.04c ||SPCC777.01c|glutathione-dependent formaldehyde d...    41   2e-04
SPBC1198.01 |||glutathione-dependent formaldehyde dehydrogenase ...    40   3e-04
SPBC1773.05c |tms1||hexitol dehydrogenase |Schizosaccharomyces p...    40   3e-04
SPBC1539.07c |||glutathione-dependent formaldehyde dehydrogenase...    39   8e-04
SPAC19A8.08 |upf2||nonsense-mediated decay protein Upf2|Schizosa...    33   0.042
SPBC16A3.02c |||mitochondrial peptidase |Schizosaccharomyces pom...    32   0.096
SPBC15C4.05 |||ATP-dependent RNA/DNA helicase |Schizosaccharomyc...    31   0.17 
SPAC1D4.14 |tho2|SPAC22F3.14c|THO complex subunit Tho2 |Schizosa...    27   2.7  
SPAC26F1.09 |gyp51||GTPase activating protein Gyp51 |Schizosacch...    27   3.6  
SPBC1D7.03 |mug80||cyclin Clg1 |Schizosaccharomyces pombe|chr 2|...    26   6.3  
SPCC1620.12c |||GTPase activating protein |Schizosaccharomyces p...    25   8.3  

>SPCC1442.16c |zta1|SPCC285.01c|NADPH quinone
           oxidoreductase/ARE-binding protein |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 329

 Score = 67.7 bits (158), Expect = 2e-12
 Identities = 43/139 (30%), Positives = 71/139 (51%), Gaps = 1/139 (0%)
 Frame = +2

Query: 272 GGLKTVKILKKPEPTVGEGEVLIRVKACGLNFQDLIVRQGAIDSPPKTPFILGFECAGEI 451
           G    ++++ K  P      ++I+    GLN+ D  +R G   +P   P+I G E AG +
Sbjct: 12  GPSSVLQVITKEIPKPAPNGLVIKNAYAGLNYIDTYLRTGLYTAP--LPYIPGKEAAGVV 69

Query: 452 EQVGENV-TNFKVGDQVVALPEYRAWAELVSVPAQYVYALPEGMSALDAVAITTNYVVAY 628
             VG+ V  +FKVGD+VV L  + A+A+  +VP   V  + E +    A A     + AY
Sbjct: 70  AAVGDKVEADFKVGDRVVYLTPFGAYAQYTNVPTTLVSKVSEKIPLKIASAALLQGLTAY 129

Query: 629 LLLFEMANLTPGKSLLVHS 685
            L+ E   +  G +++VH+
Sbjct: 130 TLIEEAYPVKTGDTVVVHA 148


>SPBC1773.06c |||alcohol dehydrogenase |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 346

 Score = 61.7 bits (143), Expect = 1e-10
 Identities = 33/81 (40%), Positives = 52/81 (64%)
 Frame = +2

Query: 260 LTGFGGLKTVKILKKPEPTVGEGEVLIRVKACGLNFQDLIVRQGAIDSPPKTPFILGFEC 439
           ++GF  LK  +  + P+  +  GEVL+++KA  LN++DLI+ +G    P + P + G + 
Sbjct: 11  ISGFDQLKPEEY-EVPQK-LNPGEVLVKLKAASLNYRDLIITKGLYPLPLQLPVVPGSDG 68

Query: 440 AGEIEQVGENVTNFKVGDQVV 502
           AG IE+VGE+V  F+ GD VV
Sbjct: 69  AGIIEKVGEDVEGFEKGDSVV 89


>SPCC13B11.04c ||SPCC777.01c|glutathione-dependent formaldehyde
           dehydrogenase|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 380

 Score = 40.7 bits (91), Expect = 2e-04
 Identities = 25/60 (41%), Positives = 31/60 (51%)
 Frame = +2

Query: 329 EVLIRVKACGLNFQDLIVRQGAIDSPPKTPFILGFECAGEIEQVGENVTNFKVGDQVVAL 508
           EV I++   G+   D     G  D     P ILG E AG +E VG  VT  +VGD V+AL
Sbjct: 39  EVRIKIVNSGVCHTDAYTLSGK-DPEGLFPVILGHEGAGIVESVGPQVTTVQVGDPVIAL 97


>SPBC1198.01 |||glutathione-dependent formaldehyde dehydrogenase
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 423

 Score = 40.3 bits (90), Expect = 3e-04
 Identities = 25/78 (32%), Positives = 42/78 (53%), Gaps = 2/78 (2%)
 Frame = +2

Query: 275 GLKTVKILKKPEPTVGEG-EVLIRVKACGL-NFQDLIVRQGAIDSPPKTPFILGFECAGE 448
           G   VKI + P+PT+    +V+++  AC + +  D  +  G +    K   ILG E  G 
Sbjct: 44  GPLNVKIAEVPKPTITHPKDVIVKTTACTICSGSDSHIFSGEMPGIEKGA-ILGHESCGI 102

Query: 449 IEQVGENVTNFKVGDQVV 502
           + + G+ V N ++GD+VV
Sbjct: 103 VAEKGDEVNNLEIGDRVV 120


>SPBC1773.05c |tms1||hexitol dehydrogenase |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 360

 Score = 40.3 bits (90), Expect = 3e-04
 Identities = 24/63 (38%), Positives = 36/63 (57%), Gaps = 2/63 (3%)
 Frame = +2

Query: 329 EVLIRVKACGLNFQDLIV-RQGAI-DSPPKTPFILGFECAGEIEQVGENVTNFKVGDQVV 502
           +V + +KA G+   D+   ++G I D   K P ILG E AG + +VG+ V++ K GD V 
Sbjct: 31  QVKVAIKATGICGSDVHYWKEGGIGDFILKKPMILGHESAGVVVEVGKGVSSLKPGDPVA 90

Query: 503 ALP 511
             P
Sbjct: 91  VEP 93


>SPBC1539.07c |||glutathione-dependent formaldehyde dehydrogenase
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 378

 Score = 38.7 bits (86), Expect = 8e-04
 Identities = 21/60 (35%), Positives = 30/60 (50%)
 Frame = +2

Query: 329 EVLIRVKACGLNFQDLIVRQGAIDSPPKTPFILGFECAGEIEQVGENVTNFKVGDQVVAL 508
           EV ++V    +   D     G +D     P +LG E AG +E +GE V N + GD V+ L
Sbjct: 36  EVRVKVDWSAVCHTDAYTLSG-VDPEGAFPIVLGHEGAGIVESIGEGVINVRPGDHVILL 94


>SPAC19A8.08 |upf2||nonsense-mediated decay protein
           Upf2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1049

 Score = 33.1 bits (72), Expect = 0.042
 Identities = 16/48 (33%), Positives = 25/48 (52%)
 Frame = +3

Query: 447 KSSKLAKMSPILRWATKWWLSPSTALGPSWCLYRPSTCTRCPKECLPW 590
           +SS L K+ P+LR+  ++WL+        +  Y PST +   K   PW
Sbjct: 154 RSSHLLKVRPLLRFLIEFWLNGVVGTPEDFVSYLPSTDSNDKKFRKPW 201


>SPBC16A3.02c |||mitochondrial peptidase |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 347

 Score = 31.9 bits (69), Expect = 0.096
 Identities = 36/141 (25%), Positives = 58/141 (41%), Gaps = 11/141 (7%)
 Frame = +2

Query: 290 KILKKPEPT-VGEGEVLIRVKACGLNFQD---LIVRQGAIDSPPKTPFILGFECAGEIEQ 457
           K L  P P  +G  +VL+ V A  +N  D   +   Q    +  K P I G++ AG +  
Sbjct: 31  KGLHIPNPAELGPYDVLVEVVATSINPLDYKLMNTYQMIAKALFKLPNIPGYDFAGRVLA 90

Query: 458 VGENVTNFKVGDQVVALPEY-RAWAELVSVPAQYV------YALPEGMSALDAVAITTNY 616
           VG  V  F    +V     + RA  +  S     V      + LP+G+S  +        
Sbjct: 91  VGSEVKEFSATQRVWGCQSFPRAGRQGGSCATHIVTGDKDVWHLPDGVSFNEGAGFGIAG 150

Query: 617 VVAYLLLFEMANLTPGKSLLV 679
           + A+ +L     + PG  L++
Sbjct: 151 LTAWEVLVRQMKVKPGTKLVI 171


>SPBC15C4.05 |||ATP-dependent RNA/DNA helicase |Schizosaccharomyces
            pombe|chr 2|||Manual
          Length = 1428

 Score = 31.1 bits (67), Expect = 0.17
 Identities = 20/62 (32%), Positives = 33/62 (53%), Gaps = 1/62 (1%)
 Frame = +2

Query: 329  EVLIRVKACGL-NFQDLIVRQGAIDSPPKTPFILGFECAGEIEQVGENVTNFKVGDQVVA 505
            EV++RVK C + + QD++ +  A+D P  T  I   E   ++  + EN    K+G  +  
Sbjct: 1055 EVVLRVKMCQMGDVQDVLGK--ALDPPSSTNIIRALEKLHQVGALSENEKLTKLGKFLSQ 1112

Query: 506  LP 511
            LP
Sbjct: 1113 LP 1114


>SPAC1D4.14 |tho2|SPAC22F3.14c|THO complex subunit Tho2
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1628

 Score = 27.1 bits (57), Expect = 2.7
 Identities = 12/33 (36%), Positives = 17/33 (51%)
 Frame = -3

Query: 556 VLGRYRHQLGPSAVLGESHHLVAHLKIGDIFAN 458
           +LGRY   L     L   +H + H+ I  I+AN
Sbjct: 425 ILGRYPFLLRAYPELSNLYHKLLHISISSIYAN 457


>SPAC26F1.09 |gyp51||GTPase activating protein Gyp51
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1031

 Score = 26.6 bits (56), Expect = 3.6
 Identities = 19/69 (27%), Positives = 28/69 (40%)
 Frame = -1

Query: 480 KLVTFSPTCSISPAHSKPKMKGVFGGESMAP*RTIKSWKFRPQAFTRIRTSPSPTVGSGF 301
           K   FSP  S+S  H K K +      +      + SW   P    R  +  SP V S +
Sbjct: 491 KNTAFSPGTSLSTNHVKTKSRSAHNNSTSPFSTAVSSW-LNP---LRYPSDKSPRVISSY 546

Query: 300 LSILTVLRP 274
           L  + + +P
Sbjct: 547 LESVFISKP 555


>SPBC1D7.03 |mug80||cyclin Clg1 |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 461

 Score = 25.8 bits (54), Expect = 6.3
 Identities = 15/45 (33%), Positives = 24/45 (53%), Gaps = 3/45 (6%)
 Frame = -1

Query: 576 PSGS-AYTYWAGTDT--SSAQARYSGRATTWSPTLKLVTFSPTCS 451
           P G  A+ +W+ + +  +S+ A YSG   T+SPT       P+ S
Sbjct: 388 PRGQKAWEWWSASYSLFTSSNATYSGEQKTYSPTTLSTNAPPSPS 432


>SPCC1620.12c |||GTPase activating protein |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 595

 Score = 25.4 bits (53), Expect = 8.3
 Identities = 13/28 (46%), Positives = 18/28 (64%), Gaps = 1/28 (3%)
 Frame = +1

Query: 346 ESLRPKLPRFD-SSSGRHRLSTEDSFHL 426
           +S R  L  F  SS+ RH++STE  FH+
Sbjct: 216 QSFRGPLEPFKYSSTTRHKVSTESIFHV 243


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,755,219
Number of Sequences: 5004
Number of extensions: 55634
Number of successful extensions: 146
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 140
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 145
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 341222980
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)