Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= bmnc12e13
         (737 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC1250.05 |rpl3002|rpl30-2, rpl30|60S ribosomal protein L30|Sc...    87   3e-18
SPAC9G1.03c |rpl3001|rpl30-1, rpl30|60S ribosomal protein L30|Sc...    82   7e-17
SPAC1834.01 |sup45||translation release factor eRF1|Schizosaccha...    29   0.52 
SPAC1782.10c |nhp2||RNA-binding protein Nhp2 |Schizosaccharomyce...    26   4.9  
SPAC1F7.01c |spt6|SPAC694.07c|transcription elongation factor Sp...    26   6.4  
SPAC1565.06c |spg1|sid3|GTPase Spg1|Schizosaccharomyces pombe|ch...    26   6.4  
SPAC23A1.05 |||sequence orphan|Schizosaccharomyces pombe|chr 1||...    25   8.5  

>SPAC1250.05 |rpl3002|rpl30-2, rpl30|60S ribosomal protein
           L30|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 117

 Score = 86.6 bits (205), Expect = 3e-18
 Identities = 41/59 (69%), Positives = 48/59 (81%)
 Frame = +2

Query: 44  APKMVAAKKQKKTIESINSRLALVMKSGKYCLGYKQTLKTLRQGKAKLVIIAKNAPPLR 220
           AP    +KK KK+ ++INS+LAL MKSGKY LGYK TLKTLR GKAKL++IA NAPPLR
Sbjct: 8   APVAAVSKKGKKSGDTINSKLALTMKSGKYVLGYKSTLKTLRSGKAKLILIAANAPPLR 66



 Score = 78.6 bits (185), Expect = 9e-16
 Identities = 33/47 (70%), Positives = 40/47 (85%)
 Frame = +1

Query: 544 RKSEIEYYALLAKTGVHHYSGNNIELGTACGKYYRVCTLAITDPGDS 684
           RKSE+EYYA+L++  VHHYSGNNI+LGTACGK +RV  LA+ D GDS
Sbjct: 66  RKSELEYYAMLSRCSVHHYSGNNIDLGTACGKLFRVGVLAVIDAGDS 112


>SPAC9G1.03c |rpl3001|rpl30-1, rpl30|60S ribosomal protein
           L30|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 109

 Score = 82.2 bits (194), Expect = 7e-17
 Identities = 38/55 (69%), Positives = 45/55 (81%)
 Frame = +2

Query: 56  VAAKKQKKTIESINSRLALVMKSGKYCLGYKQTLKTLRQGKAKLVIIAKNAPPLR 220
           V  KK KK+ ++IN++LAL MKSGKY LGYK TLKTLR GKAKL++IA N PPLR
Sbjct: 4   VVTKKSKKSGDTINAKLALTMKSGKYVLGYKSTLKTLRSGKAKLILIAGNCPPLR 58



 Score = 79.4 bits (187), Expect = 5e-16
 Identities = 34/47 (72%), Positives = 40/47 (85%)
 Frame = +1

Query: 544 RKSEIEYYALLAKTGVHHYSGNNIELGTACGKYYRVCTLAITDPGDS 684
           RKSE+EYYA+L+K  VHHY+G NI+LGTACGK +RV  LAITD GDS
Sbjct: 58  RKSELEYYAMLSKANVHHYAGTNIDLGTACGKLFRVGVLAITDAGDS 104


>SPAC1834.01 |sup45||translation release factor
           eRF1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 433

 Score = 29.5 bits (63), Expect = 0.52
 Identities = 13/42 (30%), Positives = 24/42 (57%)
 Frame = +2

Query: 68  KQKKTIESINSRLALVMKSGKYCLGYKQTLKTLRQGKAKLVI 193
           ++KK I+     ++L   SGKYC G   T+  L++G  + ++
Sbjct: 279 QEKKLIQRFFDEISL--DSGKYCFGVVDTMNALQEGAVETLL 318


>SPAC1782.10c |nhp2||RNA-binding protein Nhp2 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 154

 Score = 26.2 bits (55), Expect = 4.9
 Identities = 17/58 (29%), Positives = 32/58 (55%)
 Frame = +2

Query: 44  APKMVAAKKQKKTIESINSRLALVMKSGKYCLGYKQTLKTLRQGKAKLVIIAKNAPPL 217
           APK +  KK  KT++  + +  ++        G K+ +K +R+G+  LVI+A +  P+
Sbjct: 33  APKKLN-KKMMKTVKKASKQKHILR-------GVKEVVKAVRKGEKGLVILAGDISPM 82


>SPAC1F7.01c |spt6|SPAC694.07c|transcription elongation factor
           Spt6|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1365

 Score = 25.8 bits (54), Expect = 6.4
 Identities = 23/75 (30%), Positives = 33/75 (44%)
 Frame = +1

Query: 385 FWAYVSFKQIIFNLPLMINYKFD*IAFVS*YCFAGSQCGEPME*SLNSILHFGRKSEIEY 564
           FW+  S KQ I  L   +    D    V  +C A S   + ++  LN  +HF    +I  
Sbjct: 373 FWSLHSKKQDILKLYSDLGINDD---LVVPFCEAASSL-DAID-DLNDYIHFTYSEQIRD 427

Query: 565 YALLAKTGVHHYSGN 609
            ALL  TG+    G+
Sbjct: 428 RALLMGTGLRRPQGS 442


>SPAC1565.06c |spg1|sid3|GTPase Spg1|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 198

 Score = 25.8 bits (54), Expect = 6.4
 Identities = 15/59 (25%), Positives = 31/59 (52%), Gaps = 2/59 (3%)
 Frame = +3

Query: 162 LFDKAKQSWLSSLKMLLR*GEATEK*SFPILV--KGMHMESY*QRSMDSLCGLVKQFAK 332
           +FD +++S L+S+K   R      K + PIL+  K  H  ++ +   + +    +++AK
Sbjct: 89  MFDLSRKSTLNSIKEWYRQARGFNKTAVPILIGTKYDHFMTFPREDQEEITKQARRYAK 147


>SPAC23A1.05 |||sequence orphan|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 101

 Score = 25.4 bits (53), Expect = 8.5
 Identities = 11/30 (36%), Positives = 18/30 (60%)
 Frame = +3

Query: 456 NCIRFLILFCWITMWGTYGVVFKLDTSLWQ 545
           N + F+I+F  +T W    ++ KL  SLW+
Sbjct: 50  NTVVFIIVF--LTGWAAKSIIVKLLPSLWR 77


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,067,680
Number of Sequences: 5004
Number of extensions: 61577
Number of successful extensions: 120
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 118
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 120
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 349251756
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)