Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmnc10m12
(683 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_Q24524 Cluster: Protein singed; n=14; Endopterygota|Rep... 210 2e-53
UniRef50_Q4RZ67 Cluster: Chromosome undetermined SCAF14961, whol... 122 1e-26
UniRef50_A7RUD3 Cluster: Predicted protein; n=1; Nematostella ve... 120 3e-26
UniRef50_Q16658 Cluster: Fascin; n=49; Euteleostomi|Rep: Fascin ... 117 2e-25
UniRef50_Q6PBS5 Cluster: Fascin homolog 1-like, actin-bundling p... 113 4e-24
UniRef50_UPI0000E5925D Cluster: fascin 2 isoform 2; n=1; Homo sa... 103 4e-21
UniRef50_Q05634 Cluster: Fascin; n=1; Strongylocentrotus purpura... 86 9e-16
UniRef50_Q5C7Y0 Cluster: SJCHGC09066 protein; n=1; Schistosoma j... 70 5e-11
UniRef50_UPI00005846A8 Cluster: PREDICTED: hypothetical protein;... 62 9e-09
UniRef50_Q4RZ66 Cluster: Chromosome undetermined SCAF14961, whol... 61 2e-08
UniRef50_Q9NQT6 Cluster: Fascin-3; n=19; Mammalia|Rep: Fascin-3 ... 39 0.13
UniRef50_Q4R614 Cluster: Testis cDNA, clone: QtsA-19352, similar... 38 0.30
UniRef50_Q4PG34 Cluster: Putative uncharacterized protein; n=1; ... 34 2.8
UniRef50_Q9PCD1 Cluster: Putative uncharacterized protein; n=3; ... 33 4.9
UniRef50_P03414 Cluster: Putative uncharacterized protein PXBL-I... 33 6.5
UniRef50_Q04893 Cluster: Uncharacterized protein YMR317W; n=1; S... 33 6.5
UniRef50_Q3EBY8 Cluster: F-box protein At2g17690; n=1; Arabidops... 33 6.5
>UniRef50_Q24524 Cluster: Protein singed; n=14; Endopterygota|Rep:
Protein singed - Drosophila melanogaster (Fruit fly)
Length = 512
Score = 210 bits (513), Expect = 2e-53
Identities = 93/138 (67%), Positives = 115/138 (83%)
Frame = +2
Query: 269 MNGHSNGDINSAGDILTQNQQKGWWTIGLINSRYRYLTAETFGFKINANGTSLKKKQIWT 448
MNG +S GDI++QNQQKGWWTIGLIN +++Y+TAETFGFK+NANG SLKKKQ+WT
Sbjct: 1 MNGQGCELGHSNGDIISQNQQKGWWTIGLINGQHKYMTAETFGFKLNANGASLKKKQLWT 60
Query: 449 LEPASGNANDSMIYLRSHLDKYLAVDSFGNVTCESEEKEPGSKFHISVSDDNSGRWALRN 628
LEP+ N +S+IYLRSHL+KYL+VD FGNV CES+E++ GS+F IS+S+D SGRWAL+N
Sbjct: 61 LEPS--NTGESIIYLRSHLNKYLSVDQFGNVLCESDERDAGSRFQISISEDGSGRWALKN 118
Query: 629 VERGYFLGSSSDKLTCTA 682
RGYFLG + DKL CTA
Sbjct: 119 ESRGYFLGGTPDKLVCTA 136
>UniRef50_Q4RZ67 Cluster: Chromosome undetermined SCAF14961, whole
genome shotgun sequence; n=1; Tetraodon
nigroviridis|Rep: Chromosome undetermined SCAF14961,
whole genome shotgun sequence - Tetraodon nigroviridis
(Green puffer)
Length = 325
Score = 122 bits (293), Expect = 1e-26
Identities = 64/134 (47%), Positives = 88/134 (65%)
Frame = +2
Query: 281 SNGDINSAGDILTQNQQKGWWTIGLINSRYRYLTAETFGFKINANGTSLKKKQIWTLEPA 460
+NG N D+L Q Q GLIN +YLTAETFGFKINA+ +S+KKKQIWTLE +
Sbjct: 3 TNGASNGTSDML-QIQ------FGLINCGNKYLTAETFGFKINASASSMKKKQIWTLEQS 55
Query: 461 SGNANDSMIYLRSHLDKYLAVDSFGNVTCESEEKEPGSKFHISVSDDNSGRWALRNVERG 640
+A+ ++ LRSHL +Y+A D GNVT ++E P S+F I+ DD GRW+L++ G
Sbjct: 56 GDDASGNLFCLRSHLGRYIAADKDGNVTGDAESPGPESRFLITAHDD--GRWSLQSEAHG 113
Query: 641 YFLGSSSDKLTCTA 682
+LG + D++ C A
Sbjct: 114 RYLGGTEDRIICFA 127
>UniRef50_A7RUD3 Cluster: Predicted protein; n=1; Nematostella
vectensis|Rep: Predicted protein - Nematostella
vectensis
Length = 488
Score = 120 bits (289), Expect = 3e-26
Identities = 58/115 (50%), Positives = 79/115 (68%), Gaps = 1/115 (0%)
Frame = +2
Query: 341 WTIGLINSRY-RYLTAETFGFKINANGTSLKKKQIWTLEPASGNANDSMIYLRSHLDKYL 517
W IGL+N +YLTAETFGFK+N++G SL+KKQ W+LE G A IYL+SHL +YL
Sbjct: 7 WNIGLMNDNSSKYLTAETFGFKLNSDGVSLRKKQTWSLEQVDGEA----IYLKSHLGRYL 62
Query: 518 AVDSFGNVTCESEEKEPGSKFHISVSDDNSGRWALRNVERGYFLGSSSDKLTCTA 682
D GN+ CE+EE+E +KF + + D G+WAL++V YF G++ D+L C A
Sbjct: 63 TADPKGNLCCEAEERESNAKFTVEIKD---GKWALKSVHGAYF-GANGDQLKCVA 113
>UniRef50_Q16658 Cluster: Fascin; n=49; Euteleostomi|Rep: Fascin -
Homo sapiens (Human)
Length = 493
Score = 117 bits (282), Expect = 2e-25
Identities = 57/111 (51%), Positives = 72/111 (64%)
Frame = +2
Query: 350 GLINSRYRYLTAETFGFKINANGTSLKKKQIWTLEPASGNANDSMIYLRSHLDKYLAVDS 529
GLIN +YLTAE FGFK+NA+ +SLKKKQIWTLE A + + LRSHL +YLA D
Sbjct: 15 GLINCGNKYLTAEAFGFKVNASASSLKKKQIWTLEQPPDEAGSAAVCLRSHLGRYLAADK 74
Query: 530 FGNVTCESEEKEPGSKFHISVSDDNSGRWALRNVERGYFLGSSSDKLTCTA 682
GNVTCE E P +F I DD GRW+L++ + G + D+L+C A
Sbjct: 75 DGNVTCEREVPGPDCRFLIVAHDD--GRWSLQSEAHRRYFGGTEDRLSCFA 123
>UniRef50_Q6PBS5 Cluster: Fascin homolog 1-like, actin-bundling
protein; n=10; Gnathostomata|Rep: Fascin homolog 1-like,
actin-bundling protein - Danio rerio (Zebrafish)
(Brachydanio rerio)
Length = 290
Score = 113 bits (272), Expect = 4e-24
Identities = 59/111 (53%), Positives = 72/111 (64%)
Frame = +2
Query: 350 GLINSRYRYLTAETFGFKINANGTSLKKKQIWTLEPASGNANDSMIYLRSHLDKYLAVDS 529
GLIN RYLTAE FGFK+NA+ SLKKKQIWTLE + + ++YLRSHL +YLA D
Sbjct: 14 GLINHENRYLTAEAFGFKVNASAPSLKKKQIWTLE--QDDQDGQVVYLRSHLGRYLASDK 71
Query: 530 FGNVTCESEEKEPGSKFHISVSDDNSGRWALRNVERGYFLGSSSDKLTCTA 682
G V+CE+E KE +F I D GRWAL++ F G S D L+C A
Sbjct: 72 DGKVSCEAETKENDCRFLIVAQSD--GRWALQSETYLRFFGGSEDYLSCFA 120
>UniRef50_UPI0000E5925D Cluster: fascin 2 isoform 2; n=1; Homo
sapiens|Rep: fascin 2 isoform 2 - Homo sapiens
Length = 516
Score = 103 bits (247), Expect = 4e-21
Identities = 50/111 (45%), Positives = 67/111 (60%)
Frame = +2
Query: 350 GLINSRYRYLTAETFGFKINANGTSLKKKQIWTLEPASGNANDSMIYLRSHLDKYLAVDS 529
GL+N RYLTAE+FGFK+NA+ SLK+KQ W LEP G +++ SHL +YL+ +
Sbjct: 15 GLVNDTDRYLTAESFGFKVNASAPSLKRKQTWVLEPDPGQGT-AVLLRSSHLGRYLSAEE 73
Query: 530 FGNVTCESEEKEPGSKFHISVSDDNSGRWALRNVERGYFLGSSSDKLTCTA 682
G V CE+E +PG V GRW LR+ G F G + D+L+C A
Sbjct: 74 DGRVACEAE--QPGRDCRFLVLPQPDGRWVLRSEPHGRFFGGTEDQLSCFA 122
>UniRef50_Q05634 Cluster: Fascin; n=1; Strongylocentrotus
purpuratus|Rep: Fascin - Strongylocentrotus purpuratus
(Purple sea urchin)
Length = 496
Score = 85.8 bits (203), Expect = 9e-16
Identities = 40/113 (35%), Positives = 65/113 (57%)
Frame = +2
Query: 341 WTIGLINSRYRYLTAETFGFKINANGTSLKKKQIWTLEPASGNANDSMIYLRSHLDKYLA 520
+ GL+NS RYLTAE FG K+NA+G +LK +Q+W LE + ++ YL++ +L+
Sbjct: 8 YKFGLVNSAGRYLTAEKFGGKVNASGATLKARQVWILEQ---EESSTISYLKAPSGNFLS 64
Query: 521 VDSFGNVTCESEEKEPGSKFHISVSDDNSGRWALRNVERGYFLGSSSDKLTCT 679
D GNV C E++ + + G+WAL+NV +L + ++L C+
Sbjct: 65 ADKNGNVYCSVEDRTEDADTGFEIELQPDGKWALKNVSHQRYLACNGEELICS 117
>UniRef50_Q5C7Y0 Cluster: SJCHGC09066 protein; n=1; Schistosoma
japonicum|Rep: SJCHGC09066 protein - Schistosoma
japonicum (Blood fluke)
Length = 248
Score = 70.1 bits (164), Expect = 5e-11
Identities = 30/102 (29%), Positives = 63/102 (61%)
Frame = +2
Query: 371 RYLTAETFGFKINANGTSLKKKQIWTLEPASGNANDSMIYLRSHLDKYLAVDSFGNVTCE 550
R+LTAE F +IN +GT+L+++Q+WTL + + ++L++ L ++L+V+ G ++
Sbjct: 25 RFLTAEVFNNEINISGTALRRRQVWTL--ITDRKHPDTLFLQNSLGRFLSVNKDGKISA- 81
Query: 551 SEEKEPGSKFHISVSDDNSGRWALRNVERGYFLGSSSDKLTC 676
S + + +F + + D +G WA ++ G++L S +++C
Sbjct: 82 SLKSDSEERFRLEFAPDGTGEWAFKSDTYGFYLSGSDTQVSC 123
>UniRef50_UPI00005846A8 Cluster: PREDICTED: hypothetical protein;
n=1; Strongylocentrotus purpuratus|Rep: PREDICTED:
hypothetical protein - Strongylocentrotus purpuratus
Length = 635
Score = 62.5 bits (145), Expect = 9e-09
Identities = 39/119 (32%), Positives = 62/119 (52%), Gaps = 10/119 (8%)
Frame = +2
Query: 350 GLINSRYRYLTAETFG----FKINANGTSLKKKQIWTLEPASGNA-----NDSMIY-LRS 499
GL++S +YLT++ G ++A+ L K Q+W+LE S + +DS IY L S
Sbjct: 6 GLLSSSGKYLTSDFDGKISVSPLDASVNKLIKGQLWSLESDSNDQQVQSEDDSQIYALYS 65
Query: 500 HLDKYLAVDSFGNVTCESEEKEPGSKFHISVSDDNSGRWALRNVERGYFLGSSSDKLTC 676
YL+VD G VT ++EE +F + +G W +R+V ++G S K+ C
Sbjct: 66 TNGHYLSVDKSGKVTADAEEIGEEQRFEVDAGSGGNGAWVIRSVAHDTYMGIKSKKVLC 124
>UniRef50_Q4RZ66 Cluster: Chromosome undetermined SCAF14961, whole
genome shotgun sequence; n=1; Tetraodon
nigroviridis|Rep: Chromosome undetermined SCAF14961,
whole genome shotgun sequence - Tetraodon nigroviridis
(Green puffer)
Length = 353
Score = 61.3 bits (142), Expect = 2e-08
Identities = 34/58 (58%), Positives = 40/58 (68%)
Frame = +2
Query: 281 SNGDINSAGDILTQNQQKGWWTIGLINSRYRYLTAETFGFKINANGTSLKKKQIWTLE 454
+NG N D+L Q Q GLIN +YLTAETFGFKINA+ +S+KKKQIWTLE
Sbjct: 3 TNGASNGTSDML-QIQ------FGLINCGNKYLTAETFGFKINASASSMKKKQIWTLE 53
>UniRef50_Q9NQT6 Cluster: Fascin-3; n=19; Mammalia|Rep: Fascin-3 -
Homo sapiens (Human)
Length = 498
Score = 38.7 bits (86), Expect = 0.13
Identities = 28/112 (25%), Positives = 51/112 (45%)
Frame = +2
Query: 347 IGLINSRYRYLTAETFGFKINANGTSLKKKQIWTLEPASGNANDSMIYLRSHLDKYLAVD 526
+GLI+ YLT E + A SL ++Q W + ++ + +++ L+S YL +
Sbjct: 18 VGLISWAGTYLTFEACKNTVTATAKSLGRRQTWEILVSNEHETQAVVRLKSVQGLYLLCE 77
Query: 527 SFGNVTCESEEKEPGSKFHISVSDDNSGRWALRNVERGYFLGSSSDKLTCTA 682
G V C + + NS +W L+ + G +L S+ + CT+
Sbjct: 78 CDGTV-CYGRPRTSHHGCFLLRFHRNS-KWTLQCLISGRYLESNGKDVFCTS 127
>UniRef50_Q4R614 Cluster: Testis cDNA, clone: QtsA-19352, similar to
human fascin homolog 3, actin-bundling protein,
testicular(Strongylocentrotus purpuratus) (FSCN3),; n=1;
Macaca fascicularis|Rep: Testis cDNA, clone: QtsA-19352,
similar to human fascin homolog 3, actin-bundling
protein, testicular(Strongylocentrotus purpuratus)
(FSCN3), - Macaca fascicularis (Crab eating macaque)
(Cynomolgus monkey)
Length = 457
Score = 37.5 bits (83), Expect = 0.30
Identities = 27/112 (24%), Positives = 51/112 (45%)
Frame = +2
Query: 347 IGLINSRYRYLTAETFGFKINANGTSLKKKQIWTLEPASGNANDSMIYLRSHLDKYLAVD 526
+GLI+ YLT E + A SL ++Q W + ++ + +++ L+S YL +
Sbjct: 18 VGLISWAGTYLTFEACKNTVTATAKSLGRRQTWEILVSNEHETQAVVRLKSVQGLYLLCE 77
Query: 527 SFGNVTCESEEKEPGSKFHISVSDDNSGRWALRNVERGYFLGSSSDKLTCTA 682
G + C + + NS +W L+ + G +L S+ + CT+
Sbjct: 78 CDGTM-CYGRPRTSHHGCFLLRFHRNS-KWTLQCLISGRYLESNGKDVFCTS 127
>UniRef50_Q4PG34 Cluster: Putative uncharacterized protein; n=1;
Ustilago maydis|Rep: Putative uncharacterized protein -
Ustilago maydis (Smut fungus)
Length = 342
Score = 34.3 bits (75), Expect = 2.8
Identities = 20/73 (27%), Positives = 33/73 (45%)
Frame = +2
Query: 437 QIWTLEPASGNANDSMIYLRSHLDKYLAVDSFGNVTCESEEKEPGSKFHISVSDDNSGRW 616
Q+W G++ + +S K+L D G + E + P ++ + S +G W
Sbjct: 151 QVWIATKVPGSSEPARFTFKSAEGKFLGADKSGLLRARIEARGPQEEWTLEAS--GNGLW 208
Query: 617 ALRNVERGYFLGS 655
LR+V Y LGS
Sbjct: 209 KLRSVHNRY-LGS 220
>UniRef50_Q9PCD1 Cluster: Putative uncharacterized protein; n=3;
Xylella fastidiosa|Rep: Putative uncharacterized protein
- Xylella fastidiosa
Length = 162
Score = 33.5 bits (73), Expect = 4.9
Identities = 15/32 (46%), Positives = 22/32 (68%), Gaps = 1/32 (3%)
Frame = -1
Query: 560 FPLIRKSRYRSCR-LLGTCPSVTSSRSCYHWH 468
FP +K RYR+ R +LGT +VT ++SC W+
Sbjct: 59 FPRFKKIRYRNSRNVLGTVKNVTVNQSCGKWY 90
>UniRef50_P03414 Cluster: Putative uncharacterized protein PXBL-III;
n=32; Bovine leukemia virus|Rep: Putative
uncharacterized protein PXBL-III - Bovine leukemia virus
(isolate Japanese BLV-1) (BLV)
Length = 119
Score = 33.1 bits (72), Expect = 6.5
Identities = 17/46 (36%), Positives = 24/46 (52%), Gaps = 2/46 (4%)
Frame = -3
Query: 342 HHPFCWFWVRMSPAL--LMSPLLCPFIVIFSHSSWFTPQKKCNREI 211
HH C FWVR+ P L +P PF+ I + S P++ C E+
Sbjct: 1 HHSLCAFWVRIFPTQLPLFTPCCTPFLEIPENLS-SHPEEGCGSEV 45
>UniRef50_Q04893 Cluster: Uncharacterized protein YMR317W; n=1;
Saccharomyces cerevisiae|Rep: Uncharacterized protein
YMR317W - Saccharomyces cerevisiae (Baker's yeast)
Length = 1140
Score = 33.1 bits (72), Expect = 6.5
Identities = 18/46 (39%), Positives = 31/46 (67%)
Frame = +3
Query: 231 SAV*TSSSVKRSQ*TDTAMATSTALETSSPRTSRKDGGPSA**TLG 368
SA+ ++++ S T+T +S+A++TSSP+TS +GG S+ LG
Sbjct: 120 SALSSTNAQLSSSTTETDSISSSAIQTSSPQTSSSNGGGSSSEPLG 165
>UniRef50_Q3EBY8 Cluster: F-box protein At2g17690; n=1; Arabidopsis
thaliana|Rep: F-box protein At2g17690 - Arabidopsis
thaliana (Mouse-ear cress)
Length = 421
Score = 33.1 bits (72), Expect = 6.5
Identities = 15/53 (28%), Positives = 28/53 (52%), Gaps = 2/53 (3%)
Frame = +2
Query: 464 GNANDSMIYLRSHLDKYLAVDSFGNVTCESEEKEPGSK--FHISVSDDNSGRW 616
GN ND ++H ++ D+ GNV+C+ E++P F + +D+ +W
Sbjct: 296 GNGNDDFFAAQAHRFIHVLHDNLGNVSCKPFERDPPKTIGFKVYKNDEELLKW 348
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 636,804,681
Number of Sequences: 1657284
Number of extensions: 12013604
Number of successful extensions: 34645
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 33170
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 34607
length of database: 575,637,011
effective HSP length: 98
effective length of database: 413,223,179
effective search space used: 53305790091
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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