BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= bmnc10k23 (426 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC12B10.15c |||ribonuclease H2 complex subunit|Schizosaccharom... 29 0.40 SPAC15A10.10 |mde6||Muskelin homolog|Schizosaccharomyces pombe|c... 28 0.70 SPAC1687.22c |puf3|SPAC222.02c|RNA-binding protein Puf3 |Schizos... 27 1.6 SPBC1289.10c |||transcription factor |Schizosaccharomyces pombe|... 25 3.7 SPBC3B8.03 |||saccharopine dehydrogenase |Schizosaccharomyces po... 25 3.7 SPBC2D10.06 |rep1|rec16|MBF transcription factor complex subunit... 25 6.5 SPAPB24D3.02c |||amino acid permease, unknown 3|Schizosaccharomy... 25 6.5 SPAC1952.16 |rga9||RhoGAp, GTPase activator towards Rho/Rac/Cdc4... 25 6.5 SPAC1F3.02c |mkh1||MEK kinase |Schizosaccharomyces pombe|chr 1||... 24 8.6 >SPAC12B10.15c |||ribonuclease H2 complex subunit|Schizosaccharomyces pombe|chr 1|||Manual Length = 147 Score = 28.7 bits (61), Expect = 0.40 Identities = 10/20 (50%), Positives = 13/20 (65%) Frame = +2 Query: 77 CHIWFDTPAPFFSRYHTQTQ 136 CHI +D PAP F +H + Q Sbjct: 26 CHISYDGPAPVFEYFHDKIQ 45 >SPAC15A10.10 |mde6||Muskelin homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 716 Score = 27.9 bits (59), Expect = 0.70 Identities = 12/29 (41%), Positives = 16/29 (55%) Frame = +2 Query: 173 HIQKIQEPFIDKPPKLQNTLLLTARHSTH 259 ++QK +P DK P N L+L A TH Sbjct: 647 YLQKSMQPQFDKSPLFWNALILDAFSGTH 675 >SPAC1687.22c |puf3|SPAC222.02c|RNA-binding protein Puf3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 732 Score = 26.6 bits (56), Expect = 1.6 Identities = 11/24 (45%), Positives = 17/24 (70%) Frame = -3 Query: 421 FLLNDKVIYLQNSNKNKLFELSGL 350 FL +V+YL ++NK + FELS + Sbjct: 374 FLQQSRVLYLFHANKQRHFELSDI 397 >SPBC1289.10c |||transcription factor |Schizosaccharomyces pombe|chr 2|||Manual Length = 743 Score = 25.4 bits (53), Expect = 3.7 Identities = 16/44 (36%), Positives = 21/44 (47%), Gaps = 1/44 (2%) Frame = +2 Query: 173 HIQKIQEPFIDKPPKLQNTLLLTARHSTHPTVAK-ERSDFVACP 301 H QK + P P++QNT H THP + SDF + P Sbjct: 274 HQQK-KTPQSGSTPQMQNTTSQPTTHDTHPPKQQGPISDFRSIP 316 >SPBC3B8.03 |||saccharopine dehydrogenase |Schizosaccharomyces pombe|chr 2|||Manual Length = 450 Score = 25.4 bits (53), Expect = 3.7 Identities = 10/19 (52%), Positives = 13/19 (68%) Frame = -3 Query: 319 TVESQTRAGDEIASFLRYC 263 T+E +AG +I SFL YC Sbjct: 136 TIEEVHKAGGKIKSFLSYC 154 >SPBC2D10.06 |rep1|rec16|MBF transcription factor complex subunit Rep1|Schizosaccharomyces pombe|chr 2|||Manual Length = 472 Score = 24.6 bits (51), Expect = 6.5 Identities = 12/32 (37%), Positives = 13/32 (40%) Frame = +2 Query: 116 RYHTQTQKLGLQTPPG*TSHIQKIQEPFIDKP 211 +Y T T K L TPP HI F P Sbjct: 382 QYDTSTSKAELSTPPSKRRHIDDADLVFHSSP 413 >SPAPB24D3.02c |||amino acid permease, unknown 3|Schizosaccharomyces pombe|chr 1|||Manual Length = 543 Score = 24.6 bits (51), Expect = 6.5 Identities = 12/33 (36%), Positives = 18/33 (54%) Frame = -1 Query: 249 CLAVNSSVFCNFGGLSMNGSWIFCMCEVYPGGV 151 CLA + +F + GSWIF ++ PGG+ Sbjct: 354 CLASSREIFAFARDKGLPGSWIF--RKLTPGGI 384 >SPAC1952.16 |rga9||RhoGAp, GTPase activator towards Rho/Rac/Cdc42-like small GTPases|Schizosaccharomyces pombe|chr 1|||Manual Length = 684 Score = 24.6 bits (51), Expect = 6.5 Identities = 13/49 (26%), Positives = 22/49 (44%) Frame = +2 Query: 122 HTQTQKLGLQTPPG*TSHIQKIQEPFIDKPPKLQNTLLLTARHSTHPTV 268 H + ++ G H+ KI P + +PP + L + THPT+ Sbjct: 578 HLSAYAISIRMKDGQIRHLAKIISPCVLRPP---DDLNIIPVEDTHPTL 623 >SPAC1F3.02c |mkh1||MEK kinase |Schizosaccharomyces pombe|chr 1|||Manual Length = 1116 Score = 24.2 bits (50), Expect = 8.6 Identities = 12/35 (34%), Positives = 18/35 (51%) Frame = -3 Query: 379 KNKLFELSGLSLKSCRHDFVTVESQTRAGDEIASF 275 KNKL + S + L SC + S TR + +S+ Sbjct: 78 KNKLSKESAIQLSSCIRKTLLAPSSTRVPSKNSSY 112 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,587,134 Number of Sequences: 5004 Number of extensions: 29677 Number of successful extensions: 90 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 86 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 90 length of database: 2,362,478 effective HSP length: 66 effective length of database: 2,032,214 effective search space used: 152416050 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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