Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmnc10k23
(426 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g29280.1 68417.m04186 expressed protein ; expression supporte... 29 1.3
At3g32030.1 68416.m04070 terpene synthase/cyclase family protein... 29 1.3
At1g10170.1 68414.m01147 NF-X1 type zinc finger family protein c... 29 1.7
At5g60760.1 68418.m07623 2-phosphoglycerate kinase-related conta... 28 3.0
At4g15200.1 68417.m02329 formin homology 2 domain-containing pro... 27 5.3
At3g10100.1 68416.m01210 filament protein-related similar to YEA... 27 5.3
At2g09910.1 68415.m01029 hypothetical protein 27 5.3
At4g32200.1 68417.m04582 DNA-binding HORMA domain-containing pro... 27 7.0
At4g20730.1 68417.m03013 filament protein-related similar to Cyt... 27 7.0
At1g49890.1 68414.m05593 expressed protein contains Pfam domain,... 27 7.0
At1g22260.1 68414.m02782 expressed protein 27 7.0
At1g10390.1 68414.m01171 nucleoporin family protein contains Pfa... 27 7.0
At5g52430.1 68418.m06506 hydroxyproline-rich glycoprotein family... 26 9.3
At4g35270.1 68417.m05012 RWP-RK domain-containing protein simila... 26 9.3
At2g18940.1 68415.m02211 pentatricopeptide (PPR) repeat-containi... 26 9.3
At2g07680.1 68415.m00992 ABC transporter family protein 26 9.3
At1g67290.1 68414.m07658 glyoxal oxidase-related contains simila... 26 9.3
>At4g29280.1 68417.m04186 expressed protein ; expression supported
by MPSS
Length = 77
Score = 29.1 bits (62), Expect = 1.3
Identities = 12/28 (42%), Positives = 15/28 (53%)
Frame = -1
Query: 180 CMCEVYPGGVCNPSFCVCV*YRLKNGAG 97
C ++PG C+PS CV Y NG G
Sbjct: 31 CTIIIHPGSPCDPSDCVQYCYAEYNGVG 58
>At3g32030.1 68416.m04070 terpene synthase/cyclase family protein
contains Pfam profile: PF01397 terpene synthase family
Length = 604
Score = 29.1 bits (62), Expect = 1.3
Identities = 15/40 (37%), Positives = 24/40 (60%)
Frame = -3
Query: 415 LNDKVIYLQNSNKNKLFELSGLSLKSCRHDFVTVESQTRA 296
L+ ++L+ S LF LSLK +HDFV V++ T++
Sbjct: 16 LHQAPLFLKTSQS--LFPRPSLSLKPMKHDFVCVKATTKS 53
>At1g10170.1 68414.m01147 NF-X1 type zinc finger family protein
contains Pfam PF01422: NF-X1 type zinc finger; similar
to transcriptional repressor NF-X1 (SP:Q12986) [Homo
sapiens]; similar to EST gb|T21002
Length = 1188
Score = 28.7 bits (61), Expect = 1.7
Identities = 12/30 (40%), Positives = 19/30 (63%)
Frame = -1
Query: 267 TVGCVECLAVNSSVFCNFGGLSMNGSWIFC 178
T+ C C + ++V C+ GG S NGS ++C
Sbjct: 784 TITC-SCGRITATVPCDAGGRSANGSNVYC 812
>At5g60760.1 68418.m07623 2-phosphoglycerate kinase-related contains
weak similarity to 2-phosphoglycerate kinase (GI:467751)
[Methanothermus fervidus]
Length = 738
Score = 27.9 bits (59), Expect = 3.0
Identities = 16/50 (32%), Positives = 25/50 (50%)
Frame = +1
Query: 94 HAGAVLQPVSHADAEARVTDAARVDLAHTEDPGAVH*QAAEVTEHATINS 243
HAG L PV+ A+++AR A ++D E A A+ T+ + S
Sbjct: 229 HAGEYLDPVAVAESKARRRRAKKMDSIEDEKAKASEGGKAKNTQQTDVGS 278
>At4g15200.1 68417.m02329 formin homology 2 domain-containing
protein / FH2 domain-containing protein contains formin
homology 2 domain, Pfam:PF02181
Length = 600
Score = 27.1 bits (57), Expect = 5.3
Identities = 15/31 (48%), Positives = 20/31 (64%)
Frame = -3
Query: 385 SNKNKLFELSGLSLKSCRHDFVTVESQTRAG 293
S+K + F LS +SLK H+F T ES + AG
Sbjct: 223 SSKKRSF-LSRVSLKRNGHEFSTAESSSAAG 252
>At3g10100.1 68416.m01210 filament protein-related similar to YEAST
NUF1 protein (Spindle poly body spacer protein SPC110)
(SP:P32380) {Saccharomyces cerevisiae}; similar to
Myosin heavy chain, smooth muscle isoform (SMMHC)
(SP:P35749) {Homo sapiens}
Length = 1004
Score = 27.1 bits (57), Expect = 5.3
Identities = 9/32 (28%), Positives = 16/32 (50%)
Frame = +2
Query: 62 SFFNRCHIWFDTPAPFFSRYHTQTQKLGLQTP 157
++F +CH+WF P+ S + + TP
Sbjct: 168 AYFRQCHLWFPIPSLIISFLNRRRMAFSQLTP 199
>At2g09910.1 68415.m01029 hypothetical protein
Length = 985
Score = 27.1 bits (57), Expect = 5.3
Identities = 9/32 (28%), Positives = 16/32 (50%)
Frame = +2
Query: 62 SFFNRCHIWFDTPAPFFSRYHTQTQKLGLQTP 157
++F +CH+WF P+ S + + TP
Sbjct: 169 AYFRQCHLWFPIPSLIISFLNRRRMAFSQLTP 200
>At4g32200.1 68417.m04582 DNA-binding HORMA domain-containing
protein similar to meiotic asynaptic mutant 1
[Arabidopsis thaliana] GI:7939627, aysnaptic 1 [Brassica
oleracea var. alboglabra] GI:23506946; contains Pfam
profile PF02301: HORMA domain
Length = 1399
Score = 26.6 bits (56), Expect = 7.0
Identities = 10/30 (33%), Positives = 17/30 (56%), Gaps = 3/30 (10%)
Frame = +2
Query: 62 SFFNRCHIWFDTPA---PFFSRYHTQTQKL 142
++F +CH+WF P+ F +R H +L
Sbjct: 760 AYFRQCHLWFPIPSLIISFLNRRHMAFSQL 789
>At4g20730.1 68417.m03013 filament protein-related similar to
Cytadherence high molecular weight protein 2 (SP:P47460)
[Mycoplasma genitalium]; similar to YEAST NUF1
protein (Spindle poly body spacer protein SPC110)
(SP:P32380) {Saccharomyces cerevisiae}; also
SP|Q9UKX2|MYH2_HUMAN Myosin heavy chain, skeletal
muscle, SP|P31732|OV71_ONCVO Muscle cell intermediate
filament protein SP|P12882|MYH1_HUMAN Myosin heavy
chain, skeletal muscle,. SP|Q17107|AV71_ACAVI Muscle
cell intermediate filament protein
Length = 800
Score = 26.6 bits (56), Expect = 7.0
Identities = 10/30 (33%), Positives = 17/30 (56%), Gaps = 3/30 (10%)
Frame = +2
Query: 62 SFFNRCHIWFDTPA---PFFSRYHTQTQKL 142
++F +CH+WF P+ F +R H +L
Sbjct: 169 AYFRQCHLWFPIPSLIISFLNRRHMAFSQL 198
>At1g49890.1 68414.m05593 expressed protein contains Pfam domain,
PF04484: Family of unknown function (DUF566)
Length = 659
Score = 26.6 bits (56), Expect = 7.0
Identities = 16/49 (32%), Positives = 22/49 (44%)
Frame = +2
Query: 11 TTAT*ITQFKHGLSSPSSFFNRCHIWFDTPAPFFSRYHTQTQKLGLQTP 157
TT T T SS SS R + +P+P SR T + ++TP
Sbjct: 55 TTTTTTTTTSSSSSSSSSAILRTSKRYPSPSPLLSRSTTNSASNSIKTP 103
>At1g22260.1 68414.m02782 expressed protein
Length = 857
Score = 26.6 bits (56), Expect = 7.0
Identities = 10/22 (45%), Positives = 17/22 (77%)
Frame = -3
Query: 415 LNDKVIYLQNSNKNKLFELSGL 350
LN+K++ LQN ++ + +LSGL
Sbjct: 364 LNEKIVELQNDKESLISQLSGL 385
>At1g10390.1 68414.m01171 nucleoporin family protein contains Pfam
profiles: PF04096 nucleoporin autopeptidase, PF03093
nucleoporin FG repeat family
Length = 1041
Score = 26.6 bits (56), Expect = 7.0
Identities = 11/25 (44%), Positives = 16/25 (64%)
Frame = +2
Query: 194 PFIDKPPKLQNTLLLTARHSTHPTV 268
P +DKP ++ + LLT+RH H V
Sbjct: 704 PVVDKPAPVRISSLLTSRHLLHRRV 728
>At5g52430.1 68418.m06506 hydroxyproline-rich glycoprotein family
protein Common family member At4g25620 [Arabidopsis
thaliana]
Length = 438
Score = 26.2 bits (55), Expect = 9.3
Identities = 18/54 (33%), Positives = 24/54 (44%)
Frame = +2
Query: 95 TPAPFFSRYHTQTQKLGLQTPPG*TSHIQKIQEPFIDKPPKLQNTLLLTARHST 256
TP P FS + T+ PP + HI P + L ++L LT R ST
Sbjct: 142 TP-PVFSAFITEPSTAPYTPPPESSVHITTPSSPEVPFAQLLTSSLELTRRDST 194
>At4g35270.1 68417.m05012 RWP-RK domain-containing protein similar
to nodule inception protein GI:6448579 from (Lotus
japonicus); contains Pfam profile: PF02042 RWP-RK domain
Length = 974
Score = 26.2 bits (55), Expect = 9.3
Identities = 11/26 (42%), Positives = 16/26 (61%)
Frame = +1
Query: 271 EGTKRFRRLPSFGFRLLQNRVCKILN 348
E RF LP++GFR LQ+ + + N
Sbjct: 882 EAKVRFTLLPTWGFRELQHEIARRFN 907
>At2g18940.1 68415.m02211 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile: PF01535 PPR repeat
Length = 822
Score = 26.2 bits (55), Expect = 9.3
Identities = 15/56 (26%), Positives = 24/56 (42%)
Frame = -1
Query: 306 KRGQATKSLRSFATVGCVECLAVNSSVFCNFGGLSMNGSWIFCMCEVYPGGVCNPS 139
K +A K S GCV ++V G S + I +C++ G C+P+
Sbjct: 401 KEDEALKLFYSMKEAGCVPNTCTYNAVLSLLGKKSRSNEMIKMLCDMKSNG-CSPN 455
>At2g07680.1 68415.m00992 ABC transporter family protein
Length = 1194
Score = 26.2 bits (55), Expect = 9.3
Identities = 10/31 (32%), Positives = 17/31 (54%)
Frame = -2
Query: 302 EGRRRNRFVPSLLSDVWNVWLLIVACSVTSA 210
+G R+ P LS+ W +W ++ C V +A
Sbjct: 1046 QGSLRDNLDPLGLSEDWRIWEILDKCKVKAA 1076
>At1g67290.1 68414.m07658 glyoxal oxidase-related contains
similarity to glyoxal oxidase precursor [Phanerochaete
chrysosporium] gi|1050302|gb|AAA87594
Length = 615
Score = 26.2 bits (55), Expect = 9.3
Identities = 9/26 (34%), Positives = 19/26 (73%)
Frame = -1
Query: 261 GCVECLAVNSSVFCNFGGLSMNGSWI 184
G ++ LA+ + +C+ GGL++NG+ +
Sbjct: 166 GDIKPLALTTDTWCSSGGLTVNGTLV 191
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 8,310,030
Number of Sequences: 28952
Number of extensions: 162054
Number of successful extensions: 403
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 398
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 403
length of database: 12,070,560
effective HSP length: 74
effective length of database: 9,928,112
effective search space used: 665183504
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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